Analysis of human IgE epitope of Der f 2 with anti-Der f 2 mouse monoclonal antibodies.

abstract：:We have recently isolated and characterized human and mouse genes of a putative natural killer (NK) cell tumour-recognition protein (NK-TR) that is specifically expressed in NK cells. This gene codes for a 150 kD protein with a cyclophilin-related amino terminus followed by several positively charged domains. We repor...

journal_title：Molecular immunology

authors： Rinfret A,Anderson SK

更新日期：1993-10-01 00:00:00

abstract：:Type III interferons (IFN-λs) comprise a group of newly identified antiviral cytokines that are functionally similar to type I IFNs and elicit first-line antiviral responses. Recently, type III IFNs were identified in several species; however, little information is available about type III IFNs in ducks. We compared t...

journal_title：Molecular immunology

authors： Zhang Z,Zou T,Hu X,Jin H

更新日期：2015-12-01 00:00:00

abstract：:The membrane anchored ligand ephrinB2 belongs to the broad Eph/ephrin system and is able to activate different Eph receptors. The Eph receptors belong to the huge group of receptor-tyrosine kinases. Eph receptors as well as their corresponding ephrin ligands are cell-membrane attached proteins. Therefore, direct cell-...

journal_title：Molecular immunology

authors： Poitz DM,Ende G,Stütz B,Augstein A,Friedrichs J,Brunssen C,Werner C,Strasser RH,Jellinghaus S

更新日期：2015-12-01 00:00:00

abstract：:Activation-induced cytidine deaminase (AID) is critical for immunoglobulin (Ig) diversification in B cells. The majority of evidence supports the model that AID modifies Ig genes at the DNA level by deaminating cytosines into uracils. The mutagenic activity is largely restricted to Ig genes to avoid genomic damage in ...

journal_title：Molecular immunology

authors： Kim Y,Tian M

更新日期：2009-10-01 00:00:00

abstract：:The recovery of VDJ rearrangement is most often accomplished by PCR amplification of DNA extracted from mixtures of B-cells. Using this procedure in swine, VDJs containing chimeric V(H) genes that resemble gene-conversion products, are frequently encountered. To examine whether these chimeras could be the result of PC...

journal_title：Molecular immunology

authors： Sinkora M,Sun J,Butler JE

更新日期：2000-12-01 00:00:00

abstract：:Two anti-phenyloxazolone (phOx3) and one anti-GAT MAbs from C57BL mice are shown to be coded by VH gene 186.2. This gene has been found earlier to code for several anti-NP (NNP) antibodies (Bothwell et al., 1981) and anti-GT antibodies (Rocca-Serra et al., 1983; Carmack and Pincus, 1986). The L chain partner of the VH...

journal_title：Molecular immunology

authors： Kaartinen M,Rocca-Serra J,Mäkelä O

更新日期：1988-09-01 00:00:00

abstract：:An efficient method is described for the isolation of highly purified, IgG-free and stable guinea-pig serum C1q. The procedure includes the chromatography of EDTA-treated serum (25 mM EDTA) on CM- and DEAE-cellulose followed by gel filtration on ACA 34-Ultrogel whereby ammonium sulfate precipitation was used for conce...

journal_title：Molecular immunology

authors： Hitschold T,Golan MD,Rabs U,Loos M

更新日期：1983-02-01 00:00:00

abstract：:Dynamic interactions between major histocompatibility complex (MHC) class I heavy chains and beta2-microglobulin (beta2m) play a critical role in their stability on the cell surface, and their ability to present peptide antigens to CD8+ T-cells. A cursory review of protein sequence homologies and three-dimensional cry...

journal_title：Molecular immunology

authors： Shields MJ,Hodgson W,Ribaudo RK

更新日期：1999-06-01 00:00:00

abstract：:The molecular structure of protein and epitope mapping strategies are required to engineer the epitopes of a protein. In the present study, IgE binding regions of osmotin were identified and mutated to obtain hypoallergenic variant. The three dimensional (3-D) model of osmotin obtained by homology modeling comprised o...

journal_title：Molecular immunology

authors： Sharma P,Gaur SN,Goel N,Arora N

更新日期：2015-03-01 00:00:00

abstract：:Pulmonary exposure to innocuous aeroallergens is a common event leading to inhalation tolerance. Distinct subsets of pulmonary dendritic cells (DC) and regulatory T cells (T(Reg)) play critical roles in mediating and maintaining such tolerance. In asthmatics, the same aeroallergens drive a maladaptive, Th2-biased immu...

journal_title：Molecular immunology

authors： Köhl J,Wills-Karp M

更新日期：2007-01-01 00:00:00

abstract：:We have investigated the chemical structure of the antigenic determinant of Candida krusei cell-wall mannan. Acetolysis of the mannan, obtained by extraction with alkali and purified as copper complex, gave five oligosaccharides (from mono- to pentasaccharide) and a minor amount of an octasaccharide. Partial acetolysi...

journal_title：Molecular immunology

authors： Nishikawa A,Shinoda T,Fukazawa Y

更新日期：1982-03-01 00:00:00

abstract：:Peptides representing the amino-terminal sequence of protamines HP1 (sequence 1-12) and HP2 (sequence 1-11), the two major nuclear proteins of human sperm, have been synthesized. Rabbits were immunized either with peptide conjugated with a carrier or with free peptide. The resulting antisera were examined for their ca...

journal_title：Molecular immunology

authors： Rousseaux-Prevost R,Hublau P,Sautiere P,Rousseaux J

更新日期：1991-09-01 00:00:00

abstract：:Prolactin has several immune functions in fish however, the effects on innate and specific components of rainbow trout immunity are currently unknown. Therefore in this study, prolactin peptide (pPRL) injection in rainbow trout generated anti-PRL antibodies that were confirmed through Western blot assays of fish brain...

journal_title：Molecular immunology

authors： Villalba M,Gómez G,Torres L,Maldonado N,Espiñeira C,Payne G,Vargas-Chacoff L,Figueroa J,Yáñez A,Olavarría VH

更新日期：2020-11-01 00:00:00

abstract：:We examined the expression of T15 idiotopes (Id) on phosphorylcholine (PC)-binding monoclonal immunoglobulins and defined the structural correlates of these Id. The seven monoclonal anti-Id antibodies used as probes recognize distinct determinants that range from the antigen binding site to the CH1 domain on TEPC15. C...

journal_title：Molecular immunology

authors： Strickland FM,Gleason JT,Cerny J

更新日期：1987-06-01 00:00:00

abstract：:Tumor-associated macrophages (TAMs) are preferentially M2 skewed and promote tumor growth, angiogenesis, invasion, and/or metastasis. In this study, we have analyzed the in vitro immunomodulatory potential of a non-toxic neem leaf glycoprotein (NLGP) in reprogramming Stage III supraglottic laryngeal tumor cell lysate ...

journal_title：Molecular immunology

authors： Goswami KK,Barik S,Sarkar M,Bhowmick A,Biswas J,Bose A,Baral R

更新日期：2014-06-01 00:00:00

abstract：:Protein kinase C-associated kinase (PKK; DIK/RIP4) is an ankyrin-repeat containing serine/threonine receptor-interacting protein (RIP)-family kinase that can activate NFkappaB, and is required for keratinocyte development. In earlier studies, the expression of a catalytically inactive mutant of PKK in the B cell linea...

journal_title：Molecular immunology

authors： Moran ST,Cariappa A,Liu H,Boboila C,Shi HN,Holland PM,Peschon JJ,Pillai S

更新日期：2006-04-01 00:00:00

abstract：:The A/J murine hybridoma cell line 40-150 secretes antidigoxin antibodies with high affinity for digoxin. A first-order spontaneous mutant (40-150 A2.4) produces antibodies containing a mutation at heavy chain position 94 resulting in reduced affinity for digoxin. A second-order mutant (40-150 A2.4 P.10) derived from ...

journal_title：Molecular immunology

authors： Shaw SY,Margolies MN

更新日期：1992-04-01 00:00:00

abstract：:Forty different PCR clones encoding a llama variable heavy chain domain were analysed. The majority of these clones are derived from heavy-chain antibody cDNA in which the entire CH1 exon is absent. It appears from the amino acid within the VHH framework 1 and 3 that all the llama clones belong to the VH III family. H...

journal_title：Molecular immunology

authors： Vu KB,Ghahroudi MA,Wyns L,Muyldermans S

更新日期：1997-11-01 00:00:00

abstract：:Excessive production of pro-inflammatory cytokines such as interleukin-1β (IL-1β) plays a key role in the pathophysiological development of osteoarthritis (OA). Orexin A is an important peptide of hypothalamic origin, which has displayed its multiple biological functions in several chronic diseases via activation of i...

journal_title：Molecular immunology

authors： Wang D,Ren H,Li C,Liu F,Wang G,Shi F,Sun Y

更新日期：2018-09-01 00:00:00

abstract：:Idiotypic-like interactions between mAbs directed against cytidine (Cyd) or guanosine (Guo) nucleosides were characterized. These mAbs, Cyd-1 (IgG2b, kappa), Guo-1 (IgG1, kappa) and Guo-2 (IgG1, kappa) were derived from splenocytes of A/J mice immunized with Cyd-KLH or Guo-KLH and recognized the nucleoside base moieti...

journal_title：Molecular immunology

authors： Cottet MH,Denoyelle C,Bordenave G

更新日期：1994-01-01 00:00:00

abstract：:Whether bovine myelin basic protein (BP) and bovine spinal cord protein (SCP) cross-react at the humoral immune level was assessed with a sensitive solid-phase enzyme immunoassay. We found that a hyperimmune anti-SCP serum reacted strongly with SCP and cross-reacted nearly as well with BP. A hyperimmune anti-BP serum ...

journal_title：Molecular immunology

authors： Prokopchuk P,Ziola B,Ramshaw IA,MacPherson CF

更新日期：1984-10-01 00:00:00

abstract：:The site of the murine IgG1 molecule that regulates catabolism has recently been shown to encompass amino acids that are located at the CH2-CH3 domain interface. The CH2 and CH3 domains are connected to each other by a relatively flexible "mini-hinge" region, and flexibility in this region could clearly affect the ori...

journal_title：Molecular immunology

authors： Kim JK,Tsen MF,Ghetie V,Ward ES

更新日期：1995-05-01 00:00:00

abstract：:A sheep cDNA library was screened with a human C mu probe, and the complete nucleotide sequence of a 1923 nt cDNA was determined. It contains sequences corresponding to all the exons (VH, DH, JH, CH1, CH2, CH3 and CH4) characteristic of the immunoglobulin mu heavy chain regions. The deduced amino acid sequence shows a...

journal_title：Molecular immunology

authors： Patri S,Nau F

更新日期：1992-07-01 00:00:00

abstract：:Polymorphonuclear neutrophils (PMNs) produce and release copious amounts of reactive oxygen species (ROS) which target potential bacterial invaders but also contribute to the inflammation-associated organ injuries seen in sepsis. Calprotectin is an immune regulatory protein complex made of S100A8 and S100A9 that inhib...

journal_title：Molecular immunology

authors： Sun Y,Lu Y,Engeland CG,Gordon SC,Sroussi HY

更新日期：2013-04-01 00:00:00

abstract：:A turbidimetric method for measuring the diameters of insoluble immune complexes, based on the wavelength dependence of their ability to scatter light, was developed. The method was validated by demonstrating that it gave experimental values for the diameters of polystyrene microspheres which were in good agreement wi...

journal_title：Molecular immunology

authors： Easterbrook-Smith SB

更新日期：1993-05-01 00:00:00

abstract：:This study analyzes the gene repertoire coding for antibodies to an evolutionary novel immunogenic carbohydrate antigen in mice. The alpha-gal epitope (Gal alpha 1-3Gal beta 1-4GlcNAc-R) is an autoantigen, abundantly expressed in wild type mice, but absent in alpha 1,3galactosyltransferase knock-out (KO) mice, where i...

journal_title：Molecular immunology

authors： Chen ZC,Radic MZ,Galili U

更新日期：2000-06-01 00:00:00

abstract：:The complement system is a vital component of the immune-priveliged human eye that is always active at a low-grade level, preventing harmful intraocular injuries caused by accumulation of turnover products and controlling pathogens to preserve eye homeostasis and vision. The complement system is a double-edged sword t...

journal_title：Molecular immunology

authors： Mohlin C,Sandholm K,Ekdahl KN,Nilsson B

更新日期：2017-09-01 00:00:00

abstract：:A Brucella abortus protein with a molecular weight of 50 kDa has been shown to bind bovine immunoglobulin G from healthy, brucellosis-free animals. The Brucella immunoglobulin G binding molecule appears to be a protein, since it is susceptible to proteolysis. The protein is presumed to be located on the cell surface, ...

journal_title：Molecular immunology

authors： Bricker BJ,Tabatabai LB,Mayfield JE

更新日期：1991-01-01 00:00:00

abstract：:The NLRP3 inflammasome constitutes a major antiviral host defense mechanism during influenza virus infection. Inflammasome assembly in virus-infected cells facilitates autocatalytic processing of pro-caspase-1 and subsequent cleavage and secretion of proinflammatory cytokines IL-1β and IL-18. The NLRP3 inflammasome is...

journal_title：Molecular immunology

authors： Kuriakose T,Kanneganti TD

更新日期：2017-06-01 00:00:00

abstract：:Mannose-binding lectin (MBL) is a recognition molecule of the complement (C) system and binds to carbohydrate ligands present on a wide range of pathogenic bacteria, viruses, fungi, and parasites. MBL has been detected in the cervico-vaginal cavity where it can provide a first-line defence against infectious agents co...

journal_title：Molecular immunology

authors： Bulla R,De Seta F,Radillo O,Agostinis C,Durigutto P,Pellis V,De Santo D,Crovella S,Tedesco F

更新日期：2010-11-01 00:00:00