Molecular characterization of woodchuck CD4 (wCD4) and production of a depletion monoclonal antibody against wCD4.


:CD4 T cells play an important role in the immune response against hepatitis B virus (HBV) infection. Woodchucks represent an excellent animal model to study HBV infection. In this study, we characterized the cDNA sequence of woodchuck CD4 (wCD4). The deduced wCD4 protein has four extracellular immunoglobulin-like domains comparable to the other mammalian CD4 molecules. The important extracellular cysteine residues and the intracellular tyrosine protein kinase-binding site of wCD4 are also conserved. The deduced wCD4 protein shows 53-63% identity with the counterparts of other mammalians. Phylogenetic analysis indicates that wCD4 is closely related with the counterparts of primates. Two polyclonal antibodies (pAbs) and four monoclonal Abs (mAbs) against wCD4 were produced. Two pAbs and one mAbs (G2) were found to effectively suppress ConA induced proliferation in vitro. Anti-wCD4 mAb G2 depleted 60% of CD4 cells from healthy woodchucks, while the remaining CD4 cells responded well to ConA stimulation. This work provides a basis for studying CD4 T cell mediated immune responses against HBV infection in the woodchuck model.


Mol Immunol


Molecular immunology


Yang Y,Zhang X,Zhang C,Tao Y,Fan W,Wang Z,Wang H,Lu M,Yang D,Fiedler M,Wang B




Has Abstract


2013-11-01 00:00:00














  • Crystal structure of Drosophila PGRP-SD suggests binding to DAP-type but not lysine-type peptidoglycan.

    abstract::In Drosophila the synthesis of antimicrobial peptides in response to microbial infections is under the control of the Toll and immune deficiency (Imd) signaling pathways. The Toll signaling pathway responds mainly to Gram-positive bacterial and fungal infection while the Imd pathway mediates the response to Gram-negat...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Leone P,Bischoff V,Kellenberger C,Hetru C,Royet J,Roussel A

    更新日期:2008-05-01 00:00:00

  • Quasi-elastic light scattering of antigen-antibody complexes.

    abstract::Many biological properties of immune complexes (IC) depend upon their size. Quasi-elastic light scattering (QLS) was used to measure a mean equivalent hydrodynamic radius (Rh) and variance of the distribution of model IC composed of bovine serum albumin (BSA) as antigen (Ag) and combinations of two or three well-chara...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Yarmush DM,Murphy RM,Colton CK,Fisch M,Yarmush ML

    更新日期:1988-01-01 00:00:00

  • Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.

    abstract::Mouse monoclonal antibodies (MAbs) have been prepared against rat T cell blasts. One MAb called MRC OX-40 recognized an antigen that differed from any previously described in that its expression was detected only on T blasts that also expressed the CD4 antigen. The OX-40 MAb did not detect an activation determinant of...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Paterson DJ,Jefferies WA,Green JR,Brandon MR,Corthesy P,Puklavec M,Williams AF

    更新日期:1987-12-01 00:00:00

  • Complement control protein factor H: the good, the bad, and the inadequate.

    abstract::The complement system is an essential component of the innate immune system that participates in elimination of pathogens and altered host cells and comprises an essential link between the innate and adaptive immune system. Soluble and membrane-bound complement regulators protect cells and tissues from unintended comp...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Ferreira VP,Pangburn MK,Cortés C

    更新日期:2010-08-01 00:00:00

  • Two Litopenaeus vannamei HMGB proteins interact with transcription factors LvSTAT and LvDorsal to activate the promoter of white spot syndrome virus immediate-early gene ie1.

    abstract::White spot syndrome virus (WSSV) has caused great economic damage to shrimp aquaculture. Previous studies have shown that WSSV successfully usurps the immunity system of the host for its own gene regulation. To investigate the role of shrimp high mobility group box (HMGB) proteins in WSSV gene regulation, two Litopena...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Chen YH,Jia XT,Huang XD,Zhang S,Li M,Xie JF,Weng SP,He JG

    更新日期:2011-02-01 00:00:00

  • New insights into disease-specific absence of complement factor H related protein C in mouse models of spontaneous autoimmune diseases.

    abstract::Complement factor H (CFH) protein is an inhibitor of the alternative pathway of complement (AP) both in the fluid phase and on the surface of host cells. Mouse and human complement factor H-related (CFHR) proteins also belong to the fH family of plasma glycoproteins. The main goal of the current study was to compare t...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Mehta G,Ferreira VP,Skerka C,Zipfel PF,Banda NK

    更新日期:2014-11-01 00:00:00

  • Autoimmune VH gene family: PCR-generated murine germline VH10 genes.

    abstract::A relatively large number of variable region genes (V) contribute, via gene rearrangements with smaller numbers of additional gene elements (D and J), to generate diversity in the immune response. While some VH gene families are thought to contain 100- 1000 members, the VH10 family has only two known functioning membe...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Chao M,Voss EW Jr

    更新日期:1992-03-01 00:00:00

  • Cloning and expression analysis of two pro-inflammatory cytokines, IL-1 beta and IL-8, in haddock (Melanogrammus aeglefinus).

    abstract::This paper reports the cloning and sequencing of two pro-inflammatory cytokines, interleukin (IL)-1beta and IL-8, in haddock (Melanogrammus aeglefinus) by homology cloning. The complete transcript of the haddock IL-1beta was sequenced and contained 1043 bp, including a 762 bp open reading frame. The 3' end of the gene...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Corripio-Miyar Y,Bird S,Tsamopoulos K,Secombes CJ

    更新日期:2007-02-01 00:00:00

  • Immunochemical data suggesting a pattern for the evolution of human placental alkaline phosphatase.

    abstract::Hyperimmune absorbed rabbit antisera which were reactive with epitopes specific for individual variants of human placental alkaline phosphatase were tested for their reactivity with primate placental alkaline phosphatases. Using the three epitope-specific reactivities defined previously, we found that: epitope I is pr...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Doellgast GJ,Wei SC

    更新日期:1984-03-01 00:00:00

  • Humanized porcine VCAM-specific monoclonal antibodies with chimeric IgG2/G4 constant regions block human leukocyte binding to porcine endothelial cells.

    abstract::Discordant xenografts surviving the initial hyperacute rejection phase may be subject to cellular rejection processes mediated by infiltrating leukocytes including T cells, NK cells and monocytes. The stable adhesion of these cell types to endothelial cells is due to the molecular interaction of the integrins VLA-4 an...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Mueller JP,Giannoni MA,Hartman SL,Elliott EA,Squinto SP,Matis LA,Evans MJ

    更新日期:1997-04-01 00:00:00

  • Engineered hypoallergenic variants of osmotin demonstrate hypoallergenicity with in vitro and in vivo methods.

    abstract::The molecular structure of protein and epitope mapping strategies are required to engineer the epitopes of a protein. In the present study, IgE binding regions of osmotin were identified and mutated to obtain hypoallergenic variant. The three dimensional (3-D) model of osmotin obtained by homology modeling comprised o...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Sharma P,Gaur SN,Goel N,Arora N

    更新日期:2015-03-01 00:00:00

  • Genetic analysis of the complement factor H related 5 gene in haemolytic uraemic syndrome.

    abstract::Several mutations in the CFH gene have been described in non-Shiga-toxin-associated haemolytic uraemic syndrome (non-Stx-HUS), a rare syndrome characterized by haemolytic anaemia, thrombocytopenia and acute renal failure. Mutations in genes encoding other complement regulatory proteins, membrane cofactor protein (CD46...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Monteferrante G,Brioschi S,Caprioli J,Pianetti G,Bettinaglio P,Bresin E,Remuzzi G,Noris M

    更新日期:2007-03-01 00:00:00

  • Localisation of the monocyte-binding region on human immunoglobulin G.

    abstract::Earlier studies, which provided indirect evidence for the involvement of the C gamma 2 domain of human immunoglobulin G (IgG) in human immunoglobulin G (IgG) in human monocyte binding, have been extended to further localise the site of interaction on human IgG. A number of IgGs from several different species and fragm...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Woof JM,Partridge LJ,Jefferis R,Burton DR

    更新日期:1986-03-01 00:00:00

  • A unique Vgamma5-T-cell population in the murine mammary gland.

    abstract::Mammary epithelia and the epidermis share a common embryologic origin. Like resident epidermal T cells (ETC), a significant number of murine mammary gland T cells (MTC) express Vgamma5-T-cell receptors (TCR). MTC were assessed to see if they express the same invariant Vgamma5/Vdelta1-TCRs as those of ETC. MTC-derived ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Reardon C,Born WK,O'Brien RL

    更新日期:2006-03-01 00:00:00

  • Endogenous ligands of natural killer T cells are alpha-linked glycosylceramides.

    abstract::The nature of the endogenous ligands for natural killer T (NKT) cells has been debated for more than a decade. Because the mammalian glycosylceramide synthases are invertases, it is believed that in mammals all glycosylceramides are β anomers. However, the possibility that an alternative enzymatic pathway, an unfaithf...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Kain L,Costanzo A,Webb B,Holt M,Bendelac A,Savage PB,Teyton L

    更新日期:2015-12-01 00:00:00

  • Leucine-rich repeat-containing G-protein-coupled receptor 2 (LGR2) can regulate PO activity and AMP genes expression in Macrobrachium nipponense.

    abstract::Leucine-rich repeat-containing G-protein-coupled receptors (LGRs) form a subfamily of the large superfamily of G-protein-coupled receptors. LGRs can be divided into three groups. LGR2 from Drosophila melanogaster is involved in cuticle tanning (melanization and sclerotization). In this study, one LGR2 (MnLGR2) was ide...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Cao X,Zhang C,Zhang R,Wang K,Dai X,Huang X,Ren Q

    更新日期:2020-10-01 00:00:00

  • 14-3-3-zeta participates in TLR3-mediated TICAM-1 signal-platform formation.

    abstract::Recognition of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) is important in innate immune signaling. Toll-like receptors (TLRs) are well-characterized PRRs and are pivotal in antiviral and antitumor host defense. TIR domain-containing adaptor molecule 1 (TICAM-1, also called T...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Funami K,Matsumoto M,Obuse C,Seya T

    更新日期:2016-05-01 00:00:00

  • IRF-1 and p65 mediate upregulation of constitutive HLA-A antigen expression by hepatocellular carcinoma cells.

    abstract::Malignant transformation of hepatocytes is frequently associated with upregulation of HLA-A expression. Currently there is no information available regarding the mechanisms underlying this phenotypic change. We investigated HLA-A expression in 165 paraffin embedded tissues and 21 fresh tissues from liver cancer patien...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Shen Y,Xia M,Zhang J,Xu L,Yang J,Chen A,Miao F,Ferrone S,Xie W

    更新日期:2009-06-01 00:00:00

  • Xenotransplantation: how to overcome the complement obstacle?

    abstract::The xenotransplantation research is driven by the shortage of allografts for transplantation of patients with end-stage organ failure and by lack of success in developing suitable artificial organs. Pigs are now generally accepted to be the donor species of choice, although a possible risk for transmission of xenozoon...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Mollnes TE,Fiane AE

    更新日期:1999-03-01 00:00:00

  • Comprehensive gene expression profiling following DNA vaccination of rainbow trout against infectious hematopoietic necrosis virus.

    abstract::The DNA vaccine based on the glycoprotein gene of Infectious hematopoietic necrosis virus induces a non-specific anti-viral immune response and long-term specific immunity against IHNV. This study characterized gene expression responses associated with the early anti-viral response. Homozygous rainbow trout were injec...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Purcell MK,Nichols KM,Winton JR,Kurath G,Thorgaard GH,Wheeler P,Hansen JD,Herwig RP,Park LK

    更新日期:2006-05-01 00:00:00

  • Identification of MHC class IIbeta resistance/susceptibility alleles to Aeromonas salmonicida in brook charr (Salvelinus fontinalis).

    abstract::Pathogen-driven selection is believed to be important in the evolution and maintenance of the polymorphism of the major histocompatibility complex (MHC) genes but have been tested for very few vertebrates. In this study, we first investigate by SSCP (single strand conformational polymorphism) the diversity found at th...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Croisetière S,Tarte PD,Bernatchez L,Belhumeur P

    更新日期:2008-06-01 00:00:00

  • T cell dependent differentiation of human B cells: direct switch from IgM to IgE, and sequential switch from IgM via IgG to IgA production.

    abstract::Ig production by splenic human B cells that express different surface Ig isotypes were analysed in limiting dilution cultures. Therefore, FACS sorted IgM+, IgG+ and IgA1+ B cells were stimulated with PMA-activated EL4 thymoma cells as helper cells in the presence of IL-2 and IL-4. We found that at least every second B...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Brinkmann V,Müller S,Heusser CH

    更新日期:1992-10-01 00:00:00

  • SPI-C, a PU-box binding ETS protein expressed temporarily during B-cell development and in macrophages, contains an acidic transactivation domain located to the N-terminus.

    abstract::Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Carlsson R,Persson C,Leanderson T

    更新日期:2003-06-01 00:00:00

  • The human antibody repertoire: heavy and light chain variable region gene usage in six alloantibodies specific for human HLA class I and class II alloantigens.

    abstract::Peripheral blood B lymphocytes have been isolated from healthy individuals who were immunized with lymphocytes from HLA-incompatible donors and transformed with Epstein-Barr virus to produce human monoclonal cell lines specific for human HLA molecules. The cell lines have been previously characterized and are known to...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Andris JS,Abraham SR,Pascual V,Pistillo MP,Mantero S,Ferrara GB,Capra JD

    更新日期:1995-10-01 00:00:00

  • Interferon-gamma-induced factor binding to the interleukin-4-responsive element of CD23b promoter in human tonsillar mononuclear cells: role in transient up-regulation of the interleukin-4-induced CD23b mRNA.

    abstract::Stimulation of human tonsillar mononuclear cells with interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) rapidly induced the activation of distinct nuclear factors with different mobilities, both of which bind the IL-4 response element (IL-4RE) of CD23b promoter as examined by electrophoretic mobility shift assays ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Park HJ,So EY,Lee CE

    更新日期:1998-03-01 00:00:00

  • Immunotoxins of Pseudomonas exotoxin A (PE): effect of linkage on conjugate yield, potency, selectivity and toxicity.

    abstract::Conjugates of monoclonal antibodies and Pseudomonas exotoxin A (PE) were formed with disulfide or thioether bonds. Thioether conjugates which formed with succinimidyl 4-(N-maleimidomethyl)-cyclohexane-1-carboxylate (SMCC) modified PE and reduced antibody formed with an 80% yield of equimolar conjugate within 30 min wi...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Morgan AC Jr,Sivam G,Beaumier P,McIntyre R,Bjorn M,Abrams PG

    更新日期:1990-03-01 00:00:00

  • A novel ML protein from Manduca sexta may function as a key accessory protein for lipopolysaccharide signaling.

    abstract::Lipopolysaccharide (LPS) present on the outer membrane of Gram-negative bacteria is one of the most important pathogen-associated molecular patterns and a potent elicitor in innate immunity. In human, TLR4 (Toll-like receptor 4) and MD-2 (myeloid differiation-2) form a receptor complex to transduce the LPS signal into...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Ao JQ,Ling E,Rao XJ,Yu XQ

    更新日期:2008-05-01 00:00:00

  • Complement in BuB/BnJ mice revisited: serum C3 levels and complement opsonic activity are not elevated.

    abstract::With the exception of a few strains such as BuB/BnJ, complement activity in most inbred strains of mice is remarkably similar. The BuB/BnJ strain reportedly has high levels of complement activity and elevated serum levels of C3 and other complement proteins. However, we observed that BuB/BnJ mice have serum C3 levels ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Osmers I,Szalai AJ,Tenner AJ,Barnum SR

    更新日期:2006-04-01 00:00:00

  • The monoclonal antibody HCA2 recognises a broadly shared epitope on selected classical as well as several non-classical HLA class I molecules.

    abstract::HCA2 is a widely used monoclonal antibody, thought to be highly selective for HLA-A and -G heavy chains. We demonstrate here that it also shows affinity to HLA-B73 and HLA-E molecules on intact cells. By comparing the differences in the amino acid (AA) sequences of several HLA class I alleles that are either recognise...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Seitz C,Uchanska-Ziegler B,Zank A,Ziegler A

    更新日期:1998-09-01 00:00:00

  • HLA class II allele frequencies in the Lebanese population.

    abstract::Human leukocyte antigens (HLA) allele determination is becoming an increasingly important aspect in the field of transplantation as well as in the area of HLA association with a number of diseases. Through Lebanon's history, this country, situated at a crossroads between Europe, Asia and Africa, has been a host for va...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Samaha H,Rahal EA,Abou-Jaoude M,Younes M,Dacchache J,Hakime N

    更新日期:2003-07-01 00:00:00