Abstract：
:CD4 T cells play an important role in the immune response against hepatitis B virus (HBV) infection. Woodchucks represent an excellent animal model to study HBV infection. In this study, we characterized the cDNA sequence of woodchuck CD4 (wCD4). The deduced wCD4 protein has four extracellular immunoglobulin-like domains comparable to the other mammalian CD4 molecules. The important extracellular cysteine residues and the intracellular tyrosine protein kinase-binding site of wCD4 are also conserved. The deduced wCD4 protein shows 53-63% identity with the counterparts of other mammalians. Phylogenetic analysis indicates that wCD4 is closely related with the counterparts of primates. Two polyclonal antibodies (pAbs) and four monoclonal Abs (mAbs) against wCD4 were produced. Two pAbs and one mAbs (G2) were found to effectively suppress ConA induced proliferation in vitro. Anti-wCD4 mAb G2 depleted 60% of CD4 cells from healthy woodchucks, while the remaining CD4 cells responded well to ConA stimulation. This work provides a basis for studying CD4 T cell mediated immune responses against HBV infection in the woodchuck model.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Yang Y,Zhang X,Zhang C,Tao Y,Fan W,Wang Z,Wang H,Lu M,Yang D,Fiedler M,Wang Bdoi
10.1016/j.molimm.2013.04.006subject
Has Abstractpub_date
2013-11-01 00:00:00pages
64-71issue
1-2eissn
0161-5890issn
1872-9142pii
S0161-5890(13)00149-1journal_volume
56pub_type
杂志文章abstract：:Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00032-4
更新日期：2003-06-01 00:00:00
abstract：:This study aimed to co-culture Jurkat T lymphocytes with inactivated Mycobacterium tuberculosis (Mtb H37Ra), explore whether T lymphocytes could phagocytose H37Ra cells, and determine the underlying mechanism. Jurkat T lymphocytes were co-cultured with H37Ra cells, and confocal laser scanning microscopy, electron micr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.04.018
更新日期：2015-08-01 00:00:00
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更新日期：1994-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2010-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2020.10.011
更新日期：2020-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：1993-04-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2017-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.01.009
更新日期：2007-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：1995-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(82)90195-x
更新日期：1982-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2018.07.013
更新日期：2018-09-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(02)00090-1
更新日期：2002-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.01.003
更新日期：2012-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90122-8
更新日期：1988-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2018.04.011
更新日期：2018-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2010.09.009
更新日期：2010-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2010-02-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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更新日期：1983-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2015-12-01 00:00:00
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journal_title：Molecular immunology
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更新日期：2021-02-01 00:00:00
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journal_title：Molecular immunology
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更新日期：2017-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00148-4
更新日期：1996-03-01 00:00:00
abstract：:Despite the fact that receptors for complement on T-cells have been described many years ago the function remains unclear as is the role of complement in the T-cell response. In this review we will evaluate how the accumulated wisdom concur with the current concepts of the adaptive T-cell response. ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2005.06.027
更新日期：2006-01-01 00:00:00
abstract：:Evidence has been accumulating for a role of inflammation in the development of Alzheimer's disease (AD), a progressive neurodegenerative disorder causing a common form of dementia in the elderly. C1q, part of the initiation component of the classical complement pathway (CCP), is associated with beta-sheet, fibrillar ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.02.022
更新日期：2008-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90035-7
更新日期：1989-08-01 00:00:00
abstract：:Regulatory T cells (Tregs) are immune suppressive cells, but their roles in tumor growth have been elusive, depending on tumor type or site. Our prior study demonstrated a role of cathepsin S (CatS) in reducing Treg immunosuppressive activity. Therefore, CatS inhibition in Tregs may exacerbate tumor growth. Using mous...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.12.018
更新日期：2017-02-01 00:00:00
abstract：:Dense deposit disease (DDD) is strongly associated with the uncontrolled activation of the complement alternative pathway. Factor H (CFH)-deficient (Cfh(-/-)) mice spontaneously develop C3 deposition along the glomerular basement membrane (GBM) with subsequent development of glomerulonephritis with features of DDD, a ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.03.030
更新日期：2009-06-01 00:00:00
