Kinetics of inter-heavy chain disulfide bond formation of liganded and unliganded human immunoglobulin G by radioimmunoassay.

Abstract:

:Monoclonal antibodies (mAbs) to the hinge region of human IgG1 immunoglobulins were prepared by immunization with a proteolytic fragment of hinge segment coupled to keyhole limpet hemocyanin. A mAb, 4G3, was obtained capable of binding to intact IgG but not to partially reduced IgG. Using this mAb, inter-heavy (H) chain disulfide bond formation from partially reduced anti-tetanus antibodies (Abs) in the presence or absence of antigens (Ags) was studied by solid phase radioimmunoassay. When the Abs were partially reduced in solution and then coated to plastic plates, only 10% regeneration of inter-H chain disulfide bonds occurred after reoxidation, although 100% formation occurred in solution [Kishida et al., J. Biochem. (Tokyo) 79, 91-105 (1976)]. This difference in the extent of disulfide bond formation can be explained by the fact that there are two convertible isomers in solution, Conformer I and II, one of which (Conformer I) can form disulfide bonds but is present as a minor component. Since the motion of IgG molecules on plates is restricted by hydrophobic interactions, the two conformers are not convertible as in solution. Therefore only Conformer I which existed before coating formed inter-H chain disulfide bonds. Similar kinetic measurements were performed using plates coated with tetanus toxoid. Abs, partially reduced in solution and then allowed to react with Ags on the plate were able to completely regenerate their inter-H chain disulfide bonds, although the rate of reaction was slow. These results can also be explained by the fact that the two isomers are convertible since Ag-Ab complexes are dissociable. Ag-binding therefore did not significantly perturb conformation of the hinge segments. In addition, no difference between liganded and unliganded Abs was observed in the binding of anti-hinge mAbs. These results imply little or no contribution of the hinge region to transmission of the signal produced by Ag-binding to Fc.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Azuma T,Takeda J,Motoyama N,Okada H

doi

10.1016/0161-5890(92)90154-p

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

37-44

issue

1

eissn

0161-5890

issn

1872-9142

journal_volume

29

pub_type

杂志文章
  • IgM polymerization inhibits the Golgi-mediated processing of the mu-chain carboxy-terminal glycans.

    abstract::Secreted glycoproteins generally contain oligosaccharides of the complex type. However, several molecules have been described in which individual glycans are processed differently from one another. Folding, assembly and oligomerization could affect the maturation of certain glycans by hindering them to the Golgi proce...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(95)00132-8

    authors: Cals MM,Guenzi S,Carelli S,Simmen T,Sparvoli A,Sitia R

    更新日期:1996-01-01 00:00:00

  • Mouse IgG antibodies have subclass associated affinity differences.

    abstract::Subclasses of IgG were separated from pools of mouse sera by letting immunoglobulins absorb on protein A-Sepharose and by eluting with buffers of decreasing pH. Most donor mice were immunized with a conjugate of a hapten (NIP) and chicken gamma globulin 20 days previously. The results indicate that concentrations of I...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(83)90062-7

    authors: Sarvas HO,Seppälä IJ,Tähtinen T,Péterfy F,Mäkelä O

    更新日期:1983-03-01 00:00:00

  • Influence of gut microbiota on mouse B2 B cell ontogeny and function.

    abstract::A complex interplay between the microbiota and the host immune system is evidenced to shape the immune system throughout life, but little is known about the microbial effect on key players of the adaptive immune system, the B2 B cells. In the presented study, we have evaluated the effect of commensal bacteria on B cel...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2011.02.002

    authors: Hansson J,Bosco N,Favre L,Raymond F,Oliveira M,Metairon S,Mansourian R,Blum S,Kussmann M,Benyacoub J

    更新日期:2011-05-01 00:00:00

  • CD4 regulation in human lymphoid non-T-cells: a role for the silencer element.

    abstract::In humans, the CD4 molecule is expressed on a subset of T-cells and at various levels on myeloid and lymphoid cells. The mechanisms regulating human CD4 gene expression are yet poorly understood. We speculated that the CD4 silencer, which operates in CD8+ T-cells to repress CD4 expression, could be responsible for CD4...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2006.03.002

    authors: Mouly E,Dorival C,Pflumio F,Baillou C,Coulombel L,Levy Y,Lemoine FM,Klatzmann D,Marodon G

    更新日期:2007-01-01 00:00:00

  • Helminth-derived peptide GK-1 induces Myd88-dependent pro-inflammatory signaling events in bone marrow-derived antigen-presenting cells.

    abstract::GK-1 is an immunomodulatory, 18-aa-long peptide that has been proved to promote the activation of mouse peritoneal macrophages and LPS-pulsed mouse bone marrow-derived dendritic cells (BM-DCs). This study is aimed to explore the mechanisms underlying the activation of these antigen-presenting cells (APCs) by GK-1. In ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2020.09.015

    authors: Montero L,Cervantes-Torres J,Sciutto E,Fragoso G

    更新日期:2020-12-01 00:00:00

  • Inactivation of the KSRP gene modifies collagen antibody induced arthritis.

    abstract::The KH type splicing regulatory protein (KSRP) is a nucleic acid binding protein, which negatively regulates the stability and/or translatability of many mRNA species encoding immune-relevant proteins. As KSRP is expressed in immune cells including T and B cells, neutrophils, macrophages and dendritic cells, we wanted...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2017.05.003

    authors: Käfer R,Schrick K,Schmidtke L,Montermann E,Hobernik D,Bros M,Chen CY,Kleinert H,Pautz A

    更新日期:2017-07-01 00:00:00

  • A simple procedure for the isolation of human secretory IgA of IgA1 and IgA2 subclass by a jackfruit lectin, jacalin, affinity chromatography.

    abstract::Jackfruit lectin, jacalin, prepared from two batches of jackfruit seeds showed a different specificity in precipitating reaction in Agarose gel with various purified immunoglobulins and secretory components. Jacalin-P, extracted from jackfruit seeds from the Philippines, reacts only with serum IgA and secretory IgA of...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(87)90169-6

    authors: Kondoh H,Kobayashi K,Hagiwara K

    更新日期:1987-11-01 00:00:00

  • Comparative analysis of invertebrate Tc6 sequences that resemble the vertebrate V(D)J recombination signal sequences (RSS).

    abstract::Invertebrate cells lack the p53 recombination checkpoint but contain mobile DNA sequences that transpose by a mechanism in part shared with excision of the V(D)J recombination signal sequences (RSS). In this work, inversion, deletion, and duplication of sequences associated with an invertebrate C. elegans Tc6 element ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/s0161-5890(99)00034-6

    authors: Dreyfus DH,Gelfand EW

    更新日期:1999-05-01 00:00:00

  • Prolonged survival effects induced by immature dendritic cells and regulatory T cells in a rat liver transplantation model.

    abstract:BACKGROUND:Dendritic cells (DCs) and regulatory T (Treg) cells are crucial for inducing immune tolerance. However, the suppressive function of infused Treg cells and immature DCs (imDCs) following solid organ transplantation remains unclear. METHODS:ImDCs derived from DA-donor rats and Treg cells isolated from spleens...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2016.10.004

    authors: He W,Chen L,Zheng L,Luo L,Gao L

    更新日期:2016-11-01 00:00:00

  • NF-kappaB family of transcription factor facilitates gene conversion in chicken B cells.

    abstract::Activation-induced cytidine deaminase (AID) is critical for immunoglobulin (Ig) diversification in B cells. The majority of evidence supports the model that AID modifies Ig genes at the DNA level by deaminating cytosines into uracils. The mutagenic activity is largely restricted to Ig genes to avoid genomic damage in ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2009.07.027

    authors: Kim Y,Tian M

    更新日期:2009-10-01 00:00:00

  • Protein 4.1G binds to a unique motif within the Fc gamma RI cytoplasmic tail.

    abstract::The C-terminal domain of protein 4.1G was identified to interact with the cytosolic tail of the high affinity IgG receptor, Fc gamma RI, in yeast two-hybrid screens. Proteins of the 4.1 family have previously been found to mediate receptor/cytoskeleton interactions. In the study presented here, we show an alternativel...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2007.10.024

    authors: Beekman JM,Bakema JE,van der Poel CE,van der Linden JA,van de Winkel JG,Leusen JH

    更新日期:2008-04-01 00:00:00

  • Helper role of NK cells during the induction of anticancer responses by dendritic cells.

    abstract::Recent reports demonstrate that natural killer (NK) cells and dendritic cells (DC) support each other's activity in a positive feedback. We observed that activated NK cells induce the maturation of DCs into stable type-1 polarized DCs (DC1), characterized by up to 100-fold enhanced ability to produce IL-12p70 in respo...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.molimm.2004.07.038

    authors: Kalinski P,Giermasz A,Nakamura Y,Basse P,Storkus WJ,Kirkwood JM,Mailliard RB

    更新日期:2005-02-01 00:00:00

  • AID- and Ung-dependent generation of staggered double-strand DNA breaks in immunoglobulin class switch DNA recombination: a post-cleavage role for AID.

    abstract::Class switch DNA recombination (CSR) substitutes an immunoglobulin (Ig) constant heavy chain (C(H)) region with a different C(H) region, thereby endowing an antibody with different biological effector functions. CSR requires activation-induced cytidine deaminase (AID) and occurrence of double-strand DNA breaks (DSBs) ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2008.07.003

    authors: Zan H,Casali P

    更新日期:2008-11-01 00:00:00

  • A monoclonal antibody (RH1-38) which inhibits multiple systems of cell-mediated cytotoxicity--I. Identification of a novel epitope on a killer cell surface bimolecular complex important in the cytotoxic response.

    abstract::This paper presents the initial characterization of a mouse monoclonal antibody (RH1-38) which blocks, in the absence of complement, three different systems of cell-mediated cytotoxicity. This monoclonal antibody markedly inhibits cytotoxicity mediated by human natural killer cells, a monocyte-like cell [phorbol myris...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(85)90140-3

    authors: Hall RE

    更新日期:1985-07-01 00:00:00

  • 2-Methoxyestradiol protects against IgG immune complex-induced acute lung injury by blocking NF-κB and CCAAT/enhancer-binding protein β activities.

    abstract::Increasing evidences indicate that 2-Methoxyestradiol (2ME2) plays an essential role in protecting against inflammatory responses. However, its effect on IgG immune complex (IC)-induced acute lung injury (ALI) remains enigmatic. In the study, by using i.p. administration of 2ME2, we evaluated its influence on IgG IC-i...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2017.02.007

    authors: Yan C,Shen Y,Sun Q,Yuan D,Tang H,Gao H

    更新日期:2017-05-01 00:00:00

  • Germline transcripts of immunoglobulin light chain variable regions are structurally diverse and differentially expressed.

    abstract::The murine pre-B cell line R2-bfl, which can be induced to differentiate in vitro, was used to study germline transcription of variable regions of the light chain loci. RNA from these cells was subjected to a 3'-RACE and germline transcripts from 17 individual Vkappa gene segments belonging to 12 Vkappa families were ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/s0161-5890(03)00226-8

    authors: Düber S,Engel H,Rolink A,Kretschmer K,Weiss S

    更新日期:2003-12-01 00:00:00

  • Do Escherichia coli express Fc-receptors for immunoglobulin?

    abstract::A recent publication in Molecular Immunology by Leo and Goldman doi:10.1016/j.molimm.2009.02.024 has shown that the Escherichia coli immunoglobulin-binding (Eib) proteins are receptors for IgG and IgA. We would like to highlight important issues that were not addressed in their recent manuscript whilst providing possi...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2009.04.023

    authors: Pleass RJ

    更新日期:2009-07-01 00:00:00

  • The monoclonal antibody HCA2 recognises a broadly shared epitope on selected classical as well as several non-classical HLA class I molecules.

    abstract::HCA2 is a widely used monoclonal antibody, thought to be highly selective for HLA-A and -G heavy chains. We demonstrate here that it also shows affinity to HLA-B73 and HLA-E molecules on intact cells. By comparing the differences in the amino acid (AA) sequences of several HLA class I alleles that are either recognise...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/s0161-5890(98)00077-7

    authors: Seitz C,Uchanska-Ziegler B,Zank A,Ziegler A

    更新日期:1998-09-01 00:00:00

  • Role of MAIT cells in metabolic diseases.

    abstract::MAIT cells are innate-like T cells that are enriched in mucosal sites and tissues including adipose tissue and liver. They play an important role in immunity against microbial pathogens. Recently, it has been reported that MAIT cells could also be important in metabolic diseases and can be involved in setting up and m...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2020.12.014

    authors: Toubal A,Lehuen A

    更新日期:2021-02-01 00:00:00

  • Homology modelling and conformational analysis of IgE-binding epitopes of Ara h 3 and other legumin allergens with a cupin fold from tree nuts.

    abstract::Linear IgE-binding epitopes identified in legumin allergens of peanut (Ara h 3) and other allergenic tree nuts (Jug r 4 of walnut, Cor a 9 of hazelnut, Ana o 2 cashew nut) were mapped on three-dimensional models of the proteins built up by homology modelling. A conformational analysis revealed that consensual surface-...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2007.01.023

    authors: Barre A,Jacquet G,Sordet C,Culerrier R,Rougé P

    更新日期:2007-05-01 00:00:00

  • Fine molecular analysis of the antigenicity of the Androctonus australis hector scorpion neurotoxin II: a new antigenic epitope disclosed by the Pepscan method.

    abstract::A set of 58 overlapping rod-bound peptides was used to map the antigenic reactivity pattern of a 64-residue neurotoxin (AaH II) from the venom of the scorpion Androctonus australis hector. Five anti-toxin rabbit antisera were assayed serially for their capacity to bind to each peptide in the set. Six regions of antige...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(93)90152-2

    authors: Devaux C,Juin M,Mansuelle P,Granier C

    更新日期:1993-08-01 00:00:00

  • Role of pH-regulated antigen 1 of Candida albicans in the fungal recognition and antifungal response of human neutrophils.

    abstract::Candida albicans is an opportunistic human-pathogenic fungus, which can cause superficial but also life-threatening invasive infections. The pH-regulated antigen 1 (Pra1) of C. albicans is a surface-associated and secreted protein highly expressed in the hyphal form. Pra1 can bind to complement receptor 3 (CD11b/CD18)...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2011.07.007

    authors: Losse J,Svobodová E,Heyken A,Hube B,Zipfel PF,Józsi M

    更新日期:2011-09-01 00:00:00

  • CD3/CD28 dynabeads induce expression of tn antigen in human t cells accompanied by hypermethylation of the cosmc promoter.

    abstract::Glycosylation is an important protein post-translational modification. In this process, the intermediate product, Tn antigen, arises from somatic mutations in core1β3-galactosyltransferase-specific molecular chaperone (Cosmc), which is required for the formation of active core1β3-galactosyltransferase (T-synthase). As...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2017.06.250

    authors: Shi C,Xu X,Yu X,Du Z,Luan X,Liu D,Hu T

    更新日期:2017-10-01 00:00:00

  • Interaction of C3 and C3b with immunoglobulin G.

    abstract::Human C3b as well as native C3 were found to bind to solid phase human and rabbit IgG. Haemolytically active C3 had significantly higher binding capacity to IgG than the C3b fragment. Inhibition experiments proved that C3 and C3b have common binding sites on the Fab and Fc part of the IgG molecule but the character of...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(83)90076-7

    authors: Kulics J,Rajnavölgyi E,Füst G,Gergely J

    更新日期:1983-08-01 00:00:00

  • Protective KIR-HLA interactions for HCV infection in intravenous drug users.

    abstract::Intravenous drug use has become the principal route of hepatitis C virus (HCV) transmission due to the sharing of infected needles. In this study, we analyzed the distribution of HLA-KIR genotypes among 160 Puerto Rican intravenous drug users (IDUs) with HCV infection and 92 HCV-negative Puerto Rican IDUs. We found a ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2009.05.014

    authors: Zúñiga J,Romero V,Azocar J,Terreros D,Vargas-Rojas MI,Torres-García D,Jiménez-Alvarez L,Vargas-Alarcón G,Granados-Montiel J,Husain Z,Chung RT,Alper CA,Yunis EJ

    更新日期:2009-08-01 00:00:00

  • Potent inhibition of the classical pathway of complement by a novel C1q-binding peptide derived from the human astrovirus coat protein.

    abstract::Previous work from our laboratories has demonstrated that purified, recombinant human astrovirus coat protein (HAstV CP) binds C1q and mannose-binding lectin (MBL) inhibiting activation of the classical and lectin pathways of complement, respectively. Analysis of the 787 amino acid CP molecule revealed that residues 7...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2010.07.012

    authors: Gronemus JQ,Hair PS,Crawford KB,Nyalwidhe JO,Cunnion KM,Krishna NK

    更新日期:2010-11-01 00:00:00

  • Autologous anti-metatype immune response in rabbits.

    abstract::Rabbits hyperimmunized with fluorescyl-conjugated KLH exhibited bound ligand associated with a high affinity circulating IgG anti-fluorescein population. After cessation of immunogen administration the liganded complexes were eventually spontaneously cleared from the circulation. Individual rabbits synthesized autolog...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(92)90105-7

    authors: Voss EW Jr,Moore JK,Weidner-McGufficke KM,Denzin LK,Bedzyk WD,Voss VH

    更新日期:1992-02-01 00:00:00

  • The dual role of complement in the progression of renal disease in NZB/W F(1) mice and alternative pathway inhibition.

    abstract::Complement plays a dual role in the progression of systemic lupus erythematosus since it has important protective functions, such as the clearance of immune complexes and apoptotic cells, but is also a mediator of renal inflammation. To investigate this balance in a clinically relevant setting, we investigated how tar...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2011.09.015

    authors: Sekine H,Ruiz P,Gilkeson GS,Tomlinson S

    更新日期:2011-10-01 00:00:00

  • Selective loss of H-2Ds antigen on a murine B lymphoma due to a post-transcriptional block in expression.

    abstract::The major histocompatibility (MHC) class I antigens are coordinately expressed in most cells. However, some tumors or virus-infected cells lack expression of one MHC class I antigen, while expression of the other MHC class I antigens is unaffected. We previously described the selective expression of MHC class I antige...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(95)00092-5

    authors: Luo H,Sopchak L,Lerman SP,King SR

    更新日期:1995-10-01 00:00:00

  • Regulation of the murine inducible nitric oxide synthase gene by dexamethasone involves a heterogeneous nuclear ribonucleoprotein I (hnRNPI) dependent pathway.

    abstract::Glucocorticoids down regulate the inducible nitric oxide synthase (iNOS) gene both transcriptionally and post-transcriptionally. The post-transcriptional events are suggested to involve destabilization of the iNOS transcript although the molecular mechanisms for this effect are not known. Recently, our laboratory demo...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2007.01.029

    authors: Söderberg M,Raffalli-Mathieu F,Lang MA

    更新日期:2007-05-01 00:00:00