Abstract：
:CD20 is an important target for monoclonal antibody therapy of B-cell malignancies and for some autoimmune disorders. Though there is interest in evaluating the induction of active immunity to CD20 in the mouse model, the CD20 extracellular domain (ECD) has significant secondary and tertiary structure which make it difficult to target using peptide immunogens. We constructed, expressed, and purified a recombinant immunogen, CD20ECD-6, which displays six tandemly repeated copies of the C-terminus of the murine CD20 ECD covalently linked to maltose-binding protein. Analysis of the purified protein suggested a complex conformation as the protein migrated in significantly retarded fashion by SDS-PAGE analysis. Immunization of mice and rabbits with the CD20ECD-6 led to the induction of antibodies reactive with the C-terminal ECD peptide sequence by ELISA and more importantly, with native cell surface CD20 on the murine B-cell lymphomas, 38C13 and A20. Immunoprecipitation using the rabbit antisera and non-denaturing detergent confirmed the identity of the bound cell surface protein as murine CD20 and suggested that the cell-binding antibodies were specific for the native, folded conformation. Finally, immunization of mice with the CD20ECD-6 using Freund's or QS-21 adjuvants was shown to exert significant biological effects in vivo with the pronounced depletion of splenic B cells. The tandem-epitope immunogen represents a promising tool for eliciting and studying active autoimmunity to CD20, as a basis for potential development of new immunotherapeutics for cancer and autoimmune diseases.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Oscherwitz J,Gribbin TE,Cease KBdoi
10.1016/j.molimm.2010.01.026subject
Has Abstractpub_date
2010-04-01 00:00:00pages
1484-91issue
7-8eissn
0161-5890issn
1872-9142pii
S0161-5890(10)00038-6journal_volume
47pub_type
杂志文章abstract：:A high affinity C5 convertase is generated when a C3 convertase deposits additional C3b molecules on and around itself thereby switching the substrate specificity of C3 convertase from C3 to C5. In the present study the role of the additional C3b molecules in influencing the regulation of classical pathway C5 converta...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.07.282
更新日期：2007-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90014-b
更新日期：1991-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90115-5
更新日期：1994-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.06.010
更新日期：2020-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.11.009
更新日期：2011-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.04.010
更新日期：2017-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(86)90099-4
更新日期：1986-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(02)00070-6
更新日期：2002-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2004-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 历史文章,杂志文章,评审
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更新日期：2015-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.01.028
更新日期：2017-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90170-5
更新日期：1990-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90145-1
更新日期：1983-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00039-4
更新日期：1997-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.06.217
更新日期：2011-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2012.11.008
更新日期：2013-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 信件
doi：10.1016/j.molimm.2007.07.005
更新日期：2008-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90076-7
更新日期：1983-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.08.002
更新日期：2008-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1999-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2009-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.01.011
更新日期：2016-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2019.06.018
更新日期：2019-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.05.027
更新日期：2011-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90126-0
更新日期：1989-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.04.009
更新日期：2016-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.022
更新日期：2007-03-01 00:00:00
abstract：:The migration of dendritic cells (DCs) from the site of antigen-encounter to regional lymphoid organs is crucial for DCs to function as potent antigen-presenting cells. Matrix metalloproteinase-9 (MMP-9) is critically for DCs migration across extracellular matrix (ECM). We verified in previous studies that hypoxia dim...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.12.002
更新日期：2008-04-01 00:00:00
