Abstract:
:Peach non-specific lipid transfer protein (Pru p 3; nsLTP) has been characterized as the major food allergen in the adult Mediterranean population. Its wheat homologous protein, Tri a 14 has a relevant inhalant allergen in occupational baker's asthma. Different sensitization patterns to these allergens have been found in patients with this latter disorder. The objective of the present study was to characterize IgE epitopes of Tri a 14 and to compare them with those of Pru p 3 using three complementary strategies: the analysis of IgE-binding capacity of decapeptides bound to membrane, the identification of mimotopes using a phage display random peptide library, and the analysis of the surface electrostatic potential of both allergens. Thus, synthetic overlapping decapeptides, covering the Pru p 3 and Tri a 14 amino acid sequences, were used to identify sequential regions involved in recognition of IgE from baker's asthma patients sensitized to both nsLTPs. A phage display library was screened with total IgE from the same patients, and positive clones sequentially selected using the purified allergens, allowed to identify mimotopes (conformational epitopes) of Tri a 14 and Pru p 3. Both sequential regions and mimotopes were localized in the corresponding 3D molecular surface and their electrostatic properties were analyzed. Common sequential regions with strong IgE-binding capacity (residues 31-40 and 71-80) were identified in Tri a 14 and Pru p 3, whereas regions Tri a 14(51-60) and Pru p 3(11-20) were found specific of each allergen. A major conformational epitope (mimotope), L34H35N36R39S40S42D43G74V75L77P78Y79T80, which comprised the two common sequential epitopes, was located in Tri a 14, and a very similar one in Pru p 3. However, differences were detected on the surface electrostatic potential of both mimotopes: a first part (around residues 31-45) showed similar positive features in both allergens, whereas a second part (around residues 74-80) was markedly negative in Tri a 14 but neutral-positive in Pru p 3. Tri a 14 and Pru p 3 have a similar conformational region involved in IgE-binding, although their electrostatic features are different. Additionally, common and specific sequential IgE-binding regions were mapped in both allergens. These findings could be instrumental in understanding the cross-reactivity and specificity of sensitization to both homologous allergens.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Tordesillas L,Pacios LF,Palacin A,Quirce S,Armentia A,Barber D,Salcedo G,Diaz-Perales Adoi
10.1016/j.molimm.2009.07.028subject
Has Abstractpub_date
2009-12-01 00:00:00pages
534-40issue
2-3eissn
0161-5890issn
1872-9142pii
S0161-5890(09)00627-0journal_volume
47pub_type
杂志文章abstract::Tumor-associated macrophages (TAMs) are preferentially M2 skewed and promote tumor growth, angiogenesis, invasion, and/or metastasis. In this study, we have analyzed the in vitro immunomodulatory potential of a non-toxic neem leaf glycoprotein (NLGP) in reprogramming Stage III supraglottic laryngeal tumor cell lysate ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.01.015
更新日期:2014-06-01 00:00:00
abstract::Leishmania infantum pyridoxal kinase (PK) protein was characterized after an immunoproteomics screening performed with the sera from patients suffering visceral leishmaniasis (VL). Since it was recognized by sera of mammalian hosts infected by a viscerotropic Leishmania species, PK could emerge as a new vaccine candid...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.06.010
更新日期:2020-08-01 00:00:00
abstract::The testicular cystic structure and the abrupt morphological changes that the fish testis undergoes during the reproductive cycle (RC) make it an interesting model for studying the regulation of spermatogenesis, in particular the role of matrix metalloproteinases (Mmps). The gilthead seabream is a seasonal breeding te...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.01.031
更新日期:2008-05-01 00:00:00
abstract::REV1 plays a key role in vertebrate translesion synthesis. Although its deoxycytidyl transferase activity is dispensable for tolerance of DNA damage caused by a number of mutagens, its extreme C terminus, which interacts with other translesion polymerases and PCNA, is essential. By examining immunoglobulin diversifica...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.09.017
更新日期:2006-04-01 00:00:00
abstract::Calcium signals are crucial for diverse cellular functions including adhesion, differentiation, proliferation, effector functions and gene expression. After engagement of the B cell receptor, the intracellular calcium ion (Ca(2+)) concentration is increased promoting the activation of various signaling cascades. While...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2013.10.006
更新日期:2014-12-01 00:00:00
abstract::During T-cell regulation, T-cell receptors and CD28 lead to signaling activation, while T-lymphocyte antigen 4 (CTLA-4) is known to lead to downregulation, similar to programmed cell death-1 (PD-1). In the cytoplasmic tails of CD28 and CTLA-4, phosphoinositide 3-kinase (PI3K) binds to the consensus sequence including ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.12.002
更新日期:2020-12-29 00:00:00
abstract::A common feature of human IgG1 antibodies used for cancer treatment is that their anti-tumour efficacy requires high serum trough levels and continued therapy for several months. Treatment cycles, thereby, consume several grams of IgG1 translating into significant drug needs and costs. The basis for the low in vivo ef...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.07.010
更新日期:2006-03-01 00:00:00
abstract::The capacity of α-galactosylceramide (α-GalCer) to act as an anti-cancer agent in mice through the specific stimulation of type I NKT (iNKT) cells has prompted extensive investigation to translate this finding to the clinic. However, low frequencies of iNKT cells in cancer patients and their hypo-responsiveness to rep...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.12.010
更新日期:2021-02-01 00:00:00
abstract::Electron microscopy of specimens of C9 tilted through 90 degrees visualized this protein to be a globular ellipsoid with dimensions of 77 x 70 x 52 A. To check the congruence of this observation with physical properties of the molecule, hydrodynamic parameters for C9 were determined. From this work a frictional ratio ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90156-6
更新日期:1993-08-01 00:00:00
abstract::Art v 3, the lipid-transfer protein (LTP) of Artemisia vulgaris pollen is a relevant allergen showing frequent cross-reactivity with homologues in other plants. Here we report the identification of four full-length Art v 3 sequences obtained by cDNA cloning using mass spectrometry-based sequencing. Two isoforms, Art v...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.03.021
更新日期:2009-06-01 00:00:00
abstract::We have previously shown that immunization with SIV-, SHIV-, or HA (influenza hemagglutinin)-virus-like particles (VLPs) elicits a strong humoral immune response in mice. However, little is known about the action VLPs exert on immune effector cells, including B cells. In this study, we found that all three types of VL...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.03.008
更新日期:2009-06-01 00:00:00
abstract::Although granulocyte colony-stimulating factor(G-CSF) has pathogenic roles in several immune inflammatory diseases, its role in periodontitis has not been investigated. Here we detected local expression of G-CSF using public datasets in the Gene Expression Omnibus (GEO) database, and immune cell infiltration into ging...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.11.003
更新日期:2020-01-01 00:00:00
abstract::Candida albicans is an opportunistic human-pathogenic fungus, which can cause superficial but also life-threatening invasive infections. The pH-regulated antigen 1 (Pra1) of C. albicans is a surface-associated and secreted protein highly expressed in the hyphal form. Pra1 can bind to complement receptor 3 (CD11b/CD18)...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.07.007
更新日期:2011-09-01 00:00:00
abstract::Dense deposit disease (DDD) is strongly associated with the uncontrolled activation of the complement alternative pathway. Factor H (CFH)-deficient (Cfh(-/-)) mice spontaneously develop C3 deposition along the glomerular basement membrane (GBM) with subsequent development of glomerulonephritis with features of DDD, a ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.03.030
更新日期:2009-06-01 00:00:00
abstract::Mastocytosis is an incurable and sometimes fatal haematological disorder grossly described as the accumulation of abnormal mast cells in the bone marrow and other organs causing tissue and organ damage. The clinical manifestations of this disease are extremely variable; disease phenotypes range from indolent to aggres...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2014.03.013
更新日期:2015-01-01 00:00:00
abstract::Spondyloarthritis is the second most common form of chronic inflammatory arthritis and a unique hallmark of the disease is pathologic new bone formation. Several cytokine pathways have been genetically associated with ankylosing spondylitis (AS), the prototypic subtype of SpA, and additional evidence from human and an...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2013.07.016
更新日期:2014-01-01 00:00:00
abstract::The mast cell function-associated antigen (MAFA) is a type II membranal glycoprotein that was first identified on the surface of rat mucosal-type mast cells of the RBL-2H3 line. A C-type lectin domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) are located in the extracellular and intracellular domain...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(02)00080-9
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) or atopic eczema is a skin disease characterized with itching, increased serum level of immunoglobulin E, and peripheral eosinophilia. Thymoquinone (TQ) is an important ingredient of Nigella sativa seeds having antioxidant and anti-inflammatory effects. OBJECTIVE:Present study investi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.07.013
更新日期:2018-09-01 00:00:00
abstract::Hepcidins are antimicrobial peptides that play important roles in resisting pathogenic infection. Through hybridization of a phage library, the cDNA sequences of three hepcidin-like antimicrobial peptides (named TH1-5, TH2-2, and TH2-3) in tilapia, Oreochromis mossambicus, were determined. The complete hepcidin cDNA s...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.09.031
更新日期:2007-03-01 00:00:00
abstract::This paper presents the initial characterization of a mouse monoclonal antibody (RH1-38) which blocks, in the absence of complement, three different systems of cell-mediated cytotoxicity. This monoclonal antibody markedly inhibits cytotoxicity mediated by human natural killer cells, a monocyte-like cell [phorbol myris...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90140-3
更新日期:1985-07-01 00:00:00
abstract::The transcription factor NF-kappaB plays critical roles in immune and inflammatory responses. Here we show that filarial parasitic sheath proteins cause activation of NF-kappaB in the airway epithelial HEp-2 cell line. This activation was transient and saturable, and involved degradation of the cytoplasmic inhibitor p...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00039-0
更新日期:2000-02-01 00:00:00
abstract:BACKGROUND:Dendritic cells (DCs) and regulatory T (Treg) cells are crucial for inducing immune tolerance. However, the suppressive function of infused Treg cells and immature DCs (imDCs) following solid organ transplantation remains unclear. METHODS:ImDCs derived from DA-donor rats and Treg cells isolated from spleens...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.10.004
更新日期:2016-11-01 00:00:00
abstract::The immunosuppressive cyclic undecapeptide cyclosporine (Cs) represents a useful model for studying the molecular basis of antibody-antigen interactions. The three-dimensional structure of the Cs molecule is known and a large panel of monoclonal antibodies (mAbs) to Cs has been well characterized by cross-reactivity s...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90126-k
更新日期:1990-10-01 00:00:00
abstract::Peripheral blood B lymphocytes have been isolated from healthy individuals who were immunized with lymphocytes from HLA-incompatible donors and transformed with Epstein-Barr virus to produce human monoclonal cell lines specific for human HLA molecules. The cell lines have been previously characterized and are known to...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(95)00071-2
更新日期:1995-10-01 00:00:00
abstract::We have examined the properties of soluble guanylate cyclase activity in the human neutrophil. The enzyme showed complex regulation by metal ions. A 10-fold higher activity was observed in the presence of Mn2+ than Mg2+, while Ca2+ caused an increase in activity only in the presence of Mg2+ ion. Sodium nitroprusside (...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90138-5
更新日期:1985-07-01 00:00:00
abstract::Johne's disease is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratubercolosis (MAP) which affects ruminants worldwide and has a significant economic impact. MAP has also been associated with human Crohn's disease, although this connection is not well established. MAP is highly adapted for ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.08.017
更新日期:2017-11-01 00:00:00
abstract::In contrast to normal B lymphocytes, splenic B cells from autoimmune NZB mice express on their surface greater amounts of IgM than IgD. We have found that the predominant mu chain species present on these cells is derived from pentameric secretory IgM which can be radioiodinated during transient association with the c...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90026-0
更新日期:1988-04-01 00:00:00
abstract::Dendritic cells (DCs) are the professional antigen-presenting cells (APC) which efficiently prime the immune response or induce tolerance. We recently identified Dendritic Cell Specific TrAnsMembrane Protein (DC-STAMP), a novel 470 amino acid protein preferentially expressed by dendritic cells. Previously we demonstra...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.04.019
更新日期:2010-07-01 00:00:00
abstract::Tremendous advances in our understanding of the thrombotic microangiopathies (TMAs) have revealed distinct disease mechanisms within this heterogeneous group of diseases. As a direct result of this knowledge, both children and adults with complement-mediated TMA now enjoy higher expectations for long-term health. In t...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2015.03.246
更新日期:2015-09-01 00:00:00
abstract::Humanization of rodent mAbs by CDR-grafting (also called "reshaping") is now a standard procedure for reducing immunogenicity and recruiting human effector functions. However, the design of the humanized mAb can sometimes prove circuitous. Attempts were made to humanize L-25, a mouse antibody against the human alpha-4...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(99)00087-5
更新日期:1999-08-01 00:00:00