Abstract:
:We cloned six mycobacterial antigens into a mammalian expression vector as fusion proteins with the enhanced green fluorescent protein (EGFP). Plasmid DNA was injected intramuscularly, and the injection sites were examined 1 week later. Expression of each antigen-EGFP fusion protein was visualized as green fluorescence in muscle tissue sections. A plasmid expressing EGFP alone and a plasmid with a frameshift mutation served as positive and negative controls. Visualization of fluorescent protein in vivo was 100% specific when compared to in vitro results. In vivo sensitivity was only 37% based on individual injection sites, but increased to 100% when results from multiple injection sites were combined for each plasmid. EGFP alone was expressed in a higher proportion of myocytes than the antigen-EGFP fusion proteins (P < 0.001). There was a trend toward an inverse correlation between protein size and the proportion of myocytes with visible fluorescence (r = -0.68; P = 0.09). We compared antibody subtypes generated to Mycobacterium bovis antigen 85A, when it was expressed alone or as a fusion protein. Inclusion of EGFP modified the immune response toward a Th1 response, as indicated by the ratio of antigen 85A-specific IgG2a to IgG1 generated by each plasmid (antigen 85A alone 0.73 +/- 0.18 versus antigen 85A-EGFP 1.82 +/- 0.57, mean +/- S.D.; P < 0.01), though the magnitude of the antibody isotype shift was modest. Direct visualization of antigen-EGFP fusion proteins provided a simple and rapid method to confirm in vivo antigen expression.
journal_name
Vaccinejournal_title
Vaccineauthors
Quinn A,Jiang W,Velaz-Faircloth M,Cobb AJ,Henry SC,Frothingham Rdoi
10.1016/s0264-410x(02)00253-0subject
Has Abstractpub_date
2002-08-19 00:00:00pages
3187-92issue
25-26eissn
0264-410Xissn
1873-2518pii
S0264410X02002530journal_volume
20pub_type
杂志文章相关文献
VACCINE文献大全abstract::Group A Streptococcus (GAS) infections represent a major public health burden in both developing and developed countries. In Australia and New Zealand GAS associated diseases are serious problems in Indigenous populations and a major cause of health inequality. Political recognition of these inequalities is providing ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.05.017
更新日期:2014-06-24 00:00:00
abstract::In this study, West Nile virus (WNV) envelope (rE) protein and its domain III (rDIII) were efficiently expressed in a cost-effective system based on insect larvae as non-fermentative living biofactories. Mice immunized with the partially purified rE or rDIII elicited high antibodies titers that neutralized viral infec...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.12.081
更新日期:2011-02-17 00:00:00
abstract::The knowledge that the immune system is composed of a regulatory/suppressor arm added a new point of view to better understand the nature of several pathologies including cancer, transplants, infections and autoimmune diseases. The striking discoveries concerning molecules and cells involved in this kind of regulation...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2019.05.015
更新日期:2019-06-19 00:00:00
abstract::Genetically modified bacterial flagellin (Fla), a Toll-like receptor-5 (TLR5) ligand, was evaluated as a fusion partner for human papillomavirus (HPV) L2-based immunogens in two animal challenge models; either cutaneous inoculation of rabbits with HPV 'quasivirions' containing cottontail rabbit papillomavirus (CRPV) g...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.04.032
更新日期:2014-06-12 00:00:00
abstract::Many influenza vaccines targeted to hemagglutinin (HA) show efficient immunogenicity for protecting subjects against influenza virus infection. Major antigenic changes to HA molecules can help influenza virus to develop resistance against HA-targeted vaccines. DNA vaccines encoding conserved antigens protect animals a...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.02.074
更新日期:2007-05-22 00:00:00
abstract::The protective capacity of many currently used vaccines is based on induction of neutralizing antibodies. Many pathogens, however, have adapted themselves in different ways to escape antibody-based immune protection. In particular, for those infections against which conventional neutralizing antibody-based vaccination...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2010.08.101
更新日期:2010-11-16 00:00:00
abstract:INTRODUCTION:Cellular and humoral immune responses are both involved in protection against Plasmodium infections. The only malaria vaccine available, RTS,S, primarily induces short-lived antibodies and targets only a pre-erythrocytic stage antigen. Inclusion of erythrocytic stage targets and enhancing cellular immunoge...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.03.091
更新日期:2018-05-11 00:00:00
abstract::Actinobacillus pleuropneumoniae is a gram negative pleiomorphic rod that is the causative agent of a severe, highly infectious and often fatal pleuropneumonia in swine. We have previously reported the construction of genetically-defined stable riboflavin auxotrophs by replacement of a portion of the APP riboflavin bio...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/s0264-410x(00)00076-1
更新日期:2000-06-15 00:00:00
abstract::In this work, the propagation of the 17DD yellow fever virus in Vero cells grown on Cytodex-1 microcarriers was evaluated. After verifying that upon infection the virus adsorption step could be performed under continuous agitation, experiments were carried out in spinners and sparged lab-scale stirred-tank bioreactor ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.06.023
更新日期:2009-10-30 00:00:00
abstract::CIMAvax-EGF consists of a human recombinant epidermal growth factor (EGF), coupled to P64k, a recombinant carrier protein from N. meningitis, and Montanide ISA 51 as adjuvant. The vaccine immunization induces a specific antibody production, inhibiting the EGF/EGF-R interaction through EGF deprivation. The objective of...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.01.092
更新日期:2012-05-09 00:00:00
abstract::Although a highly effective vaccine against smallpox, vaccinia virus (VV) is not without adverse events, some of which can be life-threatening, particularly in immunocompromised individuals. We have recently demonstrated that the immunogenicity and protective efficacy of Dryvax(®) in immunocompetent mice is preserved ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.10.039
更新日期:2010-12-16 00:00:00
abstract:BACKGROUND:Rabies is a neglected zoonotic disease with a global burden of approximately 59,000 human deaths a year. Once clinical symptoms appear, rabies is almost invariably fatal; however, with timely and appropriate post-exposure prophylaxis (PEP) consisting of wound washing, vaccine, and in some cases rabies immuno...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2019.04.024
更新日期:2019-10-03 00:00:00
abstract::Several studies have emphasized the importance of an early, highly neutralizing antibody response in the clearance of Hepatitis C virus (HCV) infection. The envelope glycoprotein E2 is a major target for HCV neutralizing antibodies. Here, we compared antibody responses in mice immunized with native soluble E2 (sE2) fr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.02.009
更新日期:2011-04-05 00:00:00
abstract::The gene encoding for a major surface glycoprotein, gp63, of Leishmania major was cloned into the eukaryotic expression plasmid pCDNAI with CMV or RSV promoters. The highly susceptible Balb/c mice were injected intramuscularly with 100 micrograms/mouse of the purified plasmid. The plasmids were found to be stable in v...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)90079-5
更新日期:1994-12-01 00:00:00
abstract::The introduction of the 7-valent pneumococcal conjugate vaccine (PCV7) in the childhood immunisation programme in Norway in 2006 substantially decreased the incidence of vaccine-type (VT) invasive pneumococcal disease (IPD) in all age groups. Additionally, a slight increase in the non-vaccine (NVT) serotype IPD incide...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.10.032
更新日期:2013-12-16 00:00:00
abstract::In this article, we review three mechanisms that contribute to sustaining serum antibody levels: (i) long-lived plasma cells; (ii) persisting or cross-reactive antigen, and (iii) polyclonal activation of all memory B cells. We also present a quantitative analysis of the secondary immune response that reveals the relat...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/s0264-410x(03)00198-1
更新日期:2003-06-01 00:00:00
abstract::Rubella is a mild disease characterized by low-grade fever, and a morbilliform rash, but causes congenital defects in neonates born from mothers who suffered from rubella during the pregnancy. After many passages of wild-type rubella virus strains in various types of cultured cells, five live attenuated rubella vaccin...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.01.016
更新日期:2011-02-24 00:00:00
abstract::Recombinant plasmid DNA constructs expressing truncated or full-length dengue-1 envelope (E) with or without the pre-membrane (prM) were tested for immunogenicity in mice, as candidate dengue DNA vaccines. Two plasmids, one expressing the N-terminal 80% E and the other expressing prM and full length E were immunogenic...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(99)00570-8
更新日期:2000-05-08 00:00:00
abstract::CD4 memory T cells play a critical role in protection against repeated exposure to infectious agents such as viruses, bacteria, and helminth parasites, yet can also contribute to the aberrant immune responses associated with autoimmune and allergic reactions. Understanding the mechanisms that control effective memory ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2005.07.108
更新日期:2006-02-13 00:00:00
abstract::There is an urgent need for an effective vaccine against human disease caused by Burkholderia pseudomallei, and although a wide range of candidates have been tested in mice none provide high level protection. We considered this might reflect the inability of these vaccine candidates to protect against chronic disease....
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.02.038
更新日期:2016-03-29 00:00:00
abstract:BACKGROUND:Unbiased deep sequencing offers the potential for improved adventitious virus screening in vaccines and biotherapeutics. Successful implementation of such assays will require appropriate control materials to confirm assay performance and sensitivity. METHODS:A common reference material containing 25 target ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.12.020
更新日期:2016-04-12 00:00:00
abstract:BACKGROUND:Adjuvants like AS01B increase the immunogenicity of vaccines and generally cause increased transient reactogenicity compared with Alum. A phase II randomized trial was conducted to characterize the response to AS01B and Alum adjuvanted vaccines. A post-hoc analysis was performed to examine the associations b...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2019.02.015
更新日期:2019-03-28 00:00:00
abstract::A systematic review was performed to assess the safety and immunogenicity of the prepandemic H5N1 influenza vaccines licensed so far. A bibliographic search according to the COSI protocol was carried out and 8 of 235 potentially relevant publications were selected. Quality assessment was defined with both CASP and Jad...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2010.03.068
更新日期:2010-06-11 00:00:00
abstract::While the immunological correlates of hepatitis C virus (HCV)-specific immunity are not well understood, it is now admitted that an effective vaccine against HCV will need to induce both cellular and humoral immune responses and address viral heterogeneity to prevent immune escape. We developed a vaccine platform spec...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.05.025
更新日期:2013-03-01 00:00:00
abstract::Varicella-zoster virus (VZV) is the causative agent of varicella and zoster. The varicella vaccine was developed to control VZV infection in children. The currently available Oka vaccine strain is the only live varicella vaccine approved by the World Health Organization. We previously cloned the complete genome of the...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2014.11.001
更新日期:2016-01-04 00:00:00
abstract::Since preexisting immunity and enhanced infection rates in a clinical trial of an HIV vaccine have raised some concerns on adenovirus (Ad) serotype 5-based vaccines, we evaluated the subgroup D adenovirus serotype Ad19a for its suitability as novel viral vector vaccine against mucosal infections. In BALB/c mice, we co...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.02.075
更新日期:2018-05-03 00:00:00
abstract::Rinderpest virus causes a highly contagious and often fatal disease in domestic and wild ruminants. The surface glycoproteins, hemagglutinin (H) and fusion (F) proteins of this enveloped virus are known to confer protective immunity in cattle. We have reported the generation of a recombinant baculovirus expressing H p...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(01)00127-x
更新日期:2001-07-16 00:00:00
abstract::Bovine viral diarrhea virus (BVDV) is still one of the most serious pathogens in cattle, meriting the development of improved vaccines. Recently, we developed a new adjuvant consisting of poly[di(sodium carboxylatoethylphenoxy)]-phosphazene (PCEP), either CpG ODN or poly(I:C), and an immune defense regulator (IDR) pep...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.10.010
更新日期:2014-11-28 00:00:00
abstract::The recombinant vaccinia viruses expressing the surface protein of Neospora caninum tachyzoite, NcSAG1 or NcSRS2, were constructed. The vaccination with these recombinant viruses could protect effectively the parasite invasion in a mouse model system. The vaccine efficacy of NcSRS2 was higher than that of NcSAG1. The ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(00)00389-3
更新日期:2001-01-08 00:00:00
abstract::We evaluated the effectiveness of the Jeryl Lynn strain vaccine in a large outbreak of mumps in Navarre, Spain, 2006-2008. Each of the 241 cases of mumps occurring in children over 15 months of age born between 1998 and 2005 was compared with 5 controls individually matched by sex, birth date, district of residence an...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.02.001
更新日期:2009-03-26 00:00:00