Abstract：
:In this work, the propagation of the 17DD yellow fever virus in Vero cells grown on Cytodex-1 microcarriers was evaluated. After verifying that upon infection the virus adsorption step could be performed under continuous agitation, experiments were carried out in spinners and sparged lab-scale stirred-tank bioreactor to evaluate the use of a commercial serum-free medium (VP-SFM) and to investigate the effects of multiplicity of infection (MOI) and time of infection (TOI) on virus production. Virus titers as high as 8.4 x 10(8)pfu/mL were obtained upon infection with MOI of 0.02 and TOI of 3 days, using the serum-free medium in the sparged bioreactor.
journal_name
Vaccinejournal_title
Vaccineauthors
Souza MC,Freire MS,Schulze EA,Gaspar LP,Castilho LRdoi
10.1016/j.vaccine.2009.06.023subject
Has Abstractpub_date
2009-10-30 00:00:00pages
6420-3issue
46eissn
0264-410Xissn
1873-2518pii
S0264-410X(09)00876-7journal_volume
27pub_type
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