Enhanced immune response by amphotericin B following NS1 protein prime-oral recombinant Salmonella vaccine boost vaccination protects mice from dengue virus challenge.


:A recombinant vaccine strain SL3261/pLT105 of attenuated aroA Salmonella enterica serovar Typhimurium SL3261 strain expressing a secreted dengue virus type 2 non-structural NS1 and Yersinia pestis F1 (Caf1) fusion protein, rNS1:Caf1, was generated. Immunological evaluation was performed by prime-boost vaccine regimen. Oral immunization of mice with 1 x 10(9)cfu of SL3261/pLT105 only induced low levels of NS1-specific antibody response and protective immunity following dengue virus challenge. The parenteral NS1 protein priming-oral Salmonella boosting protocol enhanced both NS1-specific serum IgG response and protective efficacy as compared to mice immunized with each type vaccine alone. Addition of an antifungal antibiotic amphotericin B (AmB) to Salmonella vaccine further enhanced the synergic effects of prime-boost vaccine regimen on the elicited NS1-specific serum IgG response and the protective efficacy. Together, the results demonstrated that the rNS1:Caf1 producing Salmonella SL3261/pLT105 strain fails to provide effective protection as an oral vaccine alone despite co-administration of AmB as an adjuvant capable of enhancing the immune responses, and moreover, the protein priming-oral Salmonella vaccine boosting approach in combination with AmB as an immunization regimen may have the potential to be further explored as an alternative approach for dengue vaccine development.






Liu WT,Lin WT,Tsai CC,Chuang CC,Liao CL,Lin HC,Hung YW,Huang SS,Liang CC,Hsu HL,Wang HJ,Liu YT




Has Abstract


2006-07-26 00:00:00
















  • The effect of routine vaccination on invasive pneumococcal infections in Canadian children, Immunization Monitoring Program, Active 2000-2007.

    abstract::Active surveillance was conducted by the 12 centers of the Canadian Immunization Monitoring Program, Active from 2000-2007 in children 16 years of age and younger to determine the influence of 7-valent pneumococcal conjugate immunization programs on the prevalence, serotype and antibiotic resistance patterns of invasi...


    pub_type: 杂志文章,多中心研究


    authors: Bettinger JA,Scheifele DW,Kellner JD,Halperin SA,Vaudry W,Law B,Tyrrell G,Canadian Immunization Monitoring Program, Active (IMPACT).

    更新日期:2010-02-25 00:00:00

  • Enhanced protection against pneumococcal infection elicited by immunization with the combination of PspA, PspC, and ClpP.

    abstract::Immunization with a combination of several virulence-associated proteins is one of the strategies of developing effective protein-based vaccines to enhance the protection against Streptococcus pneumoniae. In this study, we evaluated the protection effects against pneumococcal infection caused by S. pneumoniae TIGR4 in...


    pub_type: 杂志文章


    authors: Cao J,Chen D,Xu W,Chen T,Xu S,Luo J,Zhao Q,Liu B,Wang D,Zhang X,Shan Y,Yin Y

    更新日期:2007-06-28 00:00:00

  • Antibody against Haemophilus influenzae protein D in patients with chronic conditions causing secondary immunodeficiency.

    abstract::Prevalence of non-typeable Haemophilus influenzae (NTHi) in the etiology of invasive infections in immunocompromised individuals is increasing. Serum IgG antibody levels to H. influenzae protein D (PD) were significantly lower in adults suffering from chronic conditions causing secondary immunodeficiency (COPD, cancer...


    pub_type: 杂志文章


    authors: Hawdon N,Biman B,McCready W,Brigden M,Malik S,Vergidis D,Kisselgoff O,Ulanova M

    更新日期:2012-02-08 00:00:00

  • The purification and protective capacity of Bordetella pertussis outer membrane proteins.

    abstract::The whole cell vaccine (WCV) of Bordetella pertussis is protective in the intracerebral (i.c.) mouse protection assay. We found a correlation between the i.c. mouse protection assay potency and the presence of the virulence-associated outer membrane proteins (OMPs) in outer membrane complexes (OMC). The virulence-asso...


    pub_type: 杂志文章


    authors: Hamstra HJ,Kuipers B,Schijf-Evers D,Loggen HG,Poolman JT

    更新日期:1995-01-01 00:00:00

  • Structural perturbation of diphtheria toxoid upon adsorption to aluminium hydroxide adjuvant.

    abstract::Aluminium-containing adjuvants are often used to enhance the potency of vaccines. In the present work we studied whether adsorption of diphtheria toxoid to colloidal aluminium hydroxide induces conformational changes of the antigen. Diphtheria toxoid has a high affinity for the aluminium hydroxide particles based on a...


    pub_type: 杂志文章


    authors: Régnier M,Metz B,Tilstra W,Hendriksen C,Jiskoot W,Norde W,Kersten G

    更新日期:2012-11-06 00:00:00

  • Pneumococcal pneumonia in the UK--how herd immunity affects the cost-effectiveness of 7-valent pneumococcal conjugate vaccine (PCV).

    abstract::This study examines the potential clinical and economic benefits for adults arising from paediatric pneumococcal vaccination in the UK. A UK birth cohort model with a 10-year horizon and using a primary 4-dose paediatric 7-valent pneumococcal conjugate vaccine (PCV) schedule was populated with 1999 morbidity and morta...


    pub_type: 杂志文章


    authors: McIntosh ED,Conway P,Willingham J,Hollingsworth R,Lloyd A

    更新日期:2005-02-25 00:00:00

  • The role of the vaccines industry in Mission Grand Convergence.

    abstract::The vast majority of vaccines used throughout the world are supplied by the private sector. It is essential therefore that the industry is closely engaged in future policy developments at a national and international level and is able to respond to the changing needs and priorities that may be required to ensure the s...


    pub_type: 杂志文章


    authors: Almond J,Medaglini D

    更新日期:2017-01-20 00:00:00

  • Public opinion on childhood immunisations in Iceland.

    abstract:INTRODUCTION:In recent years, vaccine preventable diseases such as measles and pertussis have been re-emerging in Western countries, maybe because of decreasing participation in childhood vaccination programs in some countries. There is clear evidence for vaccine efficacy and the risk of adverse effects is low. This ne...


    pub_type: 杂志文章


    authors: Óskarsson Ý,Guðnason Þ,Jónsdóttir GA,Kristinsson KG,Briem H,Haraldsson Á

    更新日期:2015-12-16 00:00:00

  • Strategy for the control of hepatitis B virus infection in the Middle East and North Africa. The Middle East Regional Study Group.

    abstract::Hepatitis B virus (HBV) infection and HBV-associated diseases are prevalent in many parts of the Middle East and North Africa. Postnatal horizontal childhood transmission appears to be the predominant method by which high hepatitis B carrier rates are maintained. To control hepatitis B in this region, mass immunizatio...


    pub_type: 杂志文章


    authors: Toukan A

    更新日期:1990-03-01 00:00:00

  • Safety and immunogenicity of an 8 year interval heterologous prime-boost influenza A/H7N7-H7N9 vaccination.

    abstract:BACKGROUND:Influenza A/H7N9 viruses are undergoing antigenic drift since their emergence in 2013, and vaccination strategies are needed for pandemic preparedness. Two doses of adjuvanted monovalent inactivated influenza A/H7N9 vaccine (IIV1 A/H7N9) are needed for optimal serological responses. However, administering 2 ...


    pub_type: 杂志文章


    authors: El Sahly HM,Atmar RL,Patel SM,Bellamy A,Liu L,Hong W,Zhu H,Guan Y,Keitel WA,DMID 13-0033 Vaccine Study Group.

    更新日期:2019-05-01 00:00:00

  • Memory T cells and vaccines.

    abstract::T lymphocytes play a central role in the generation of a protective immune response in many microbial infections. After immunization, dendritic cells take up microbial antigens and traffic to draining lymph nodes where they present processed antigens to naïve T cells. These naïve T cells are stimulated to proliferate ...


    pub_type: 杂志文章,评审


    authors: Esser MT,Marchese RD,Kierstead LS,Tussey LG,Wang F,Chirmule N,Washabaugh MW

    更新日期:2003-01-17 00:00:00

  • There are viruses and viruses.....

    abstract::Life has its problems. To succeed it has to survive: to survive it has to sacrifice its identity. And viruses have their fingers in the essence of it all. Consider the situation. There are useful genes. Such genes promote the survival of the individuals which house them. It is not unreasonable, therefore, to expect th...


    pub_type: 社论


    authors: Spier RE

    更新日期:1991-12-01 00:00:00

  • Detection of measles vaccine in the throat of a vaccinated child.

    abstract::Measles vaccine is widely used, most often in association with mumps and rubella vaccines. We report here the case of a child presenting with fever 8 days after vaccination with a measles-mumps-rubella vaccine. Measles virus was isolated in a throat swab taken 4 days after fever onset. This virus was then further gene...


    pub_type: 杂志文章


    authors: Morfin F,Beguin A,Lina B,Thouvenot D

    更新日期:2002-02-22 00:00:00

  • Biodegradable microspheres as controlled-release tetanus toxoid delivery systems.

    abstract::Purified tetanus toxoid, a high-molecular-weight protein, was entrapped within poly(L-lactic acid) (PLA) and poly(D,L-lactic/glycolic acid) (PLGA) microspheres prepared by either a solvent extraction or a solvent evaporation method carried out in a multiple emulsion system (water-in-oil-in-water). The physical integri...


    pub_type: 杂志文章


    authors: Alonso MJ,Gupta RK,Min C,Siber GR,Langer R

    更新日期:1994-03-01 00:00:00

  • Establishment of gene-vaccinated skin grafting against Toxoplasma gondii infection in mice.

    abstract::Vaccine effects of in vivo gene-vaccinated skin graft were evaluated against Toxoplasma gondii (T. gondii) infection. By using a gene gun, cDNA coding T. gondii SAG1 molecule was intracutaneously vaccinated into C57BL/6 (B6; a susceptible strain), BALB/c (a resistant strain) and (C57BL/6 x BALB/c) F1 (CBF1) mice, and ...


    pub_type: 杂志文章


    authors: Saito S,Aosai F,Rikihisa N,Mun HS,Norose K,Chen M,Kuroki T,Asano T,Ochiai T,Hata H,Ichinose M,Yano A

    更新日期:2001-02-28 00:00:00

  • Potential of Mw as a prophylactic vaccine against pulmonary tuberculosis.

    abstract::Mycobacterium w (Mw), is a cultivable, non-pathogenic mycobacterium and has been tried extensively as an immunomodulator in leprosy. This has been found to be safe and has shown beneficial immunoprophylactic effect in population based, double blind placebo controlled trials in North India. These effects were also obse...


    pub_type: 杂志文章,随机对照试验


    authors: Katoch K,Singh P,Adhikari T,Benara SK,Singh HB,Chauhan DS,Sharma VD,Lavania M,Sachan AS,Katoch VM

    更新日期:2008-02-26 00:00:00

  • Antigenic properties of the merozoite surface protein 1 gene of Plasmodium vivax.

    abstract::Plasmodium vivax is responsible for an approximate 35 million yearly human cases of malaria. Unfortunately, due to the low mortality rate associated with it and the difficulties of continuously in vitro culturing of this parasite, vaccine development against this human malaria has been largely neglected. In here, the ...


    pub_type: 杂志文章


    authors: de Oliveira CI,Wunderlich G,Levitus G,Soares IS,Rodrigues MM,Tsuji M,del Portillo HA

    更新日期:1999-08-06 00:00:00

  • Delivery of the Pertactin/P.69 polypeptide of Bordetella pertussis using an attenuated Salmonella typhimurium vaccine strain: expression levels and immune response.

    abstract::Pertactin/P.69, a surface-associated polypeptide antigen of Bordetella pertussis, was expressed in a Salmonella typhimurium aroA aroD vaccine strain, BRD509, using different expression systems, and the immune response in mice against these constructs was compared. Initially, Pertactin/P.69 was expressed on the surface...


    pub_type: 杂志文章


    authors: Anderson R,Dougan G,Roberts M

    更新日期:1996-10-01 00:00:00

  • Vaccination coverage against 2009 seasonal influenza in chronically ill children and adults: analysis of population registries in primary care in Madrid (Spain).

    abstract::Using electronic clinical records in primary care (ECRPC) of the entire population living in the Autonomous Community of Madrid, Spain (5,102,568 persons) as a data source, this study aimed to ascertain seasonal anti-influenza vaccination coverage in the chronically ill at-risk children (aged 6 months to 14 years) and...


    pub_type: 杂志文章


    authors: Rodríguez-Rieiro C,Domínguez-Berjón MF,Esteban-Vasallo MD,Sánchez-Perruca L,Astray-Mochales J,Fornies DI,Ordoñez DB,Jiménez-García R

    更新日期:2010-08-31 00:00:00

  • E-health use, vaccination knowledge and perception of own risk: drivers of vaccination uptake in medical students.

    abstract:OBJECTIVE:was to improve understanding of mechanisms contributing to healthcare personnel's (HCP) reluctance to get vaccinated against seasonal influenza. We assessed the role of several drivers: vaccination knowledge, vaccination recommendations and the role of the Internet (so-called e-health) in creating vaccination...


    pub_type: 杂志文章


    authors: Betsch C,Wicker S

    更新日期:2012-02-01 00:00:00

  • Persistence of protection through 33 months of age provided by immunization in infancy with two three-component acellular pertussis vaccines. Stage II Working Group.

    abstract::A large, randomized, placebo-controlled clinical trial in Italy on two three-component pertussis vaccines, given as DTaP in infancy, one manufactured by SmithKline and Beecham (SB) and one by Chiron Biocine (CB), found each vaccine to be 84% efficacious through the average age of 24 months. The cohort of children enro...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Salmaso S,Mastrantonio P,Wassilak SG,Giuliano M,Anemona A,Giammanco A,Tozzi AE,Ciofi degli Atti ML,Greco D

    更新日期:1998-08-01 00:00:00

  • Towards developing standardized protocols for evaluation of cellular mucosal immune responses - Recommendations from a DAIDS/NIH workshop, June 15-16, 2009.

    abstract::Although 80% of HIV infections occur through mucosal routes and vaccine strategies need to be designed for inducing protective immune responses at the site of the viral entry, it has proven to be very challenging to measure these responses. A 2-day workshop was convened by Division of AIDS, National Institutes of Heal...




    authors: Mehra V,Musib R,Schito ML

    更新日期:2010-07-05 00:00:00

  • Socioeconomic factors play a more important role in childhood vaccination coverage than parental perceptions: a cross-sectional study in Greece.

    abstract::To identify predictive factors of complete and age-appropriate vaccination status in Greece, we conducted a cross-sectional study, using stratified cluster sampling, among children attending the first year of the Greek Grammar school (about 6 years of age) and their parents/guardians. Almost 88% (N=3878) of pupils in ...


    pub_type: 杂志文章


    authors: Danis K,Georgakopoulou T,Stavrou T,Laggas D,Panagiotopoulos T

    更新日期:2010-02-17 00:00:00

  • Th2-polarisation of cellular immune memory to neonatal pertussis vaccination.

    abstract::Current infant vaccination against pertussis in North America and Australia requires three doses of vaccines including diphtheria, tetanus and acellular pertussis antigens (DTaP) at 2, 4 and 6 months of age. Interest is growing in the possibility that vaccination at birth might provide earlier protection of infants, b...


    pub_type: 杂志文章


    authors: White OJ,Rowe J,Richmond P,Marshall H,McIntyre P,Wood N,Holt PG

    更新日期:2010-03-19 00:00:00

  • Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis.

    abstract::A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in a...


    pub_type: 杂志文章


    authors: Kajikawa A,Satoh E,Leer RJ,Yamamoto S,Igimi S

    更新日期:2007-05-04 00:00:00

  • Short and long-term immunogenicity and safety following the 23-valent polysaccharide pneumococcal vaccine in juvenile idiopathic arthritis patients under conventional DMARDs with or without anti-TNF therapy.

    abstract:OBJECTIVES:To assess immunogenicity and safety of the 23-valent polysaccharide pneumococcal vaccine (PPV23) in juvenile idiopathic arthritis (JIA) patients under conventional DMARDs with or without anti-TNF therapy. The influences of demographic data, disease activity and treatment on immune response and the potential ...


    pub_type: 杂志文章


    authors: Aikawa NE,França IL,Ribeiro AC,Sallum AM,Bonfa E,Silva CA

    更新日期:2015-01-29 00:00:00

  • Assessing the adequacy of attenuation of genetically modified malaria parasite vaccine candidates.

    abstract::The critical first step in the clinical development of a malaria vaccine, based on live-attenuated Plasmodium falciparum sporozoites, is the guarantee of complete arrest in the liver. We report on an approach for assessing adequacy of attenuation of genetically attenuated sporozoites in vivo using the Plasmodium bergh...


    pub_type: 杂志文章


    authors: Annoura T,Ploemen IH,van Schaijk BC,Sajid M,Vos MW,van Gemert GJ,Chevalley-Maurel S,Franke-Fayard BM,Hermsen CC,Gego A,Franetich JF,Mazier D,Hoffman SL,Janse CJ,Sauerwein RW,Khan SM

    更新日期:2012-03-30 00:00:00

  • Process development for the mass production of Ehrlichia ruminantium.

    abstract::This work describes the optimization of a cost-effective process for the production of an inactivated bacterial vaccine against heartwater and the first attempt to produce the causative agent of this disease, the rickettsia Ehrlichia ruminantium (ER), using stirred tanks. In vitro, it is possible to produce ER using c...


    pub_type: 杂志文章


    authors: Marcelino I,Sousa MF,Veríssimo C,Cunha AE,Carrondo MJ,Alves PM

    更新日期:2006-03-06 00:00:00

  • Improved timely birth dose coverage of hepatitis B vaccine narrows the socio-economic inequality in western China in 2011-2016.

    abstract:OBJECTIVE:To compare timely birth dose (TBD) coverage of hepatitis B vaccine and socio-economic inequality distribution of TBD coverage between 2011 and 2016 in rural areas of western China. METHODS:In western China, using multi-stage probability proportion to size sampling, 2633 and 1929 children aged 0-35 months wit...


    pub_type: 杂志文章


    authors: Liu X,Yang C,Zhong Q,Song Q,Huang X,Yang Y,Huang Y,Wang Y,Zhou H

    更新日期:2018-06-22 00:00:00

  • Pandemic unadjuvanted influenza A (H1N1) vaccine in dermatomyositis and polymyositis: immunogenicity independent of therapy and no harmful effect in disease.

    abstract::The goal of the present study was to evaluate the influence of the influenza A H1N1/2009 vaccine on dermatomyositis/polymyositis (DM/PM) disease parameters and the potential deleterious effect of therapy on immune response. Thirty-seven DM and 21 PM patients (Bohan and Peter's criteria) were gender- and age-matched to...


    pub_type: 杂志文章


    authors: Shinjo SK,de Moraes JC,Levy-Neto M,Aikawa NE,de Medeiros Ribeiro AC,Schahin Saad CG,Precioso A,Silva CA,Bonfá E

    更新日期:2012-12-17 00:00:00