Hepatitis C virus soluble E2 in combination with QuilA and CpG ODN induces neutralizing antibodies in mice.


:Several studies have emphasized the importance of an early, highly neutralizing antibody response in the clearance of Hepatitis C virus (HCV) infection. The envelope glycoprotein E2 is a major target for HCV neutralizing antibodies. Here, we compared antibody responses in mice immunized with native soluble E2 (sE2) from the H77 1a isolate coupled with different adjuvants or combinations of adjuvants. Adjuvanting sE2 with Freund's, monophosphoryl lipid A (MPL), cytosine phosphorothioate guanine oligodeoxynucleotide (CpG ODN), or alpha-galactosylceramide (αGalCer) derivatives elicited only moderate antibody responses. In contrast, immunizations with sE2 and QuilA elicited exceptionally high anti-E2 antibody titers. Sera from these mice effectively neutralized HCV pseudoparticles (HCVpp) 1a entry. Moreover, the combination of QuilA and CpG ODN further enhanced neutralizing antibody titers wherein cross-neutralization of HCVpp 4 was observed. We conclude that the combination of QuilA and CpG ODN is a promising adjuvant combination that should be further explored for the development of an HCV subunit vaccine. Our work also emphasizes that the ideal combination of adjuvant and immunogen has to be determined empirically.






Naarding MA,Falkowska E,Xiao H,Dragic T




Has Abstract


2011-04-05 00:00:00
















  • Safety and immunological efficacy of a prostate cancer plasmid DNA vaccine encoding prostatic acid phosphatase (PAP).

    abstract::Prostatic acid phosphatase (PAP) is a prostate tumor antigen currently being investigated as a target antigen in several human vaccine trials, some with evidence of clinical benefit. We have previously demonstrated that plasmid DNA vaccines encoding either human or rat PAP can elicit antigen-specific cellular and humo...


    pub_type: 杂志文章


    authors: Johnson LE,Frye TP,Arnot AR,Marquette C,Couture LA,Gendron-Fitzpatrick A,McNeel DG

    更新日期:2006-01-16 00:00:00

  • Avian paramyxovirus serotypes 2-9 (APMV-2-9) vary in the ability to induce protective immunity in chickens against challenge with virulent Newcastle disease virus (APMV-1).

    abstract::The avian paramyxoviruses (APMVs) belong to the genus Avulavirus of family Paramyxoviridae. The APMVs are classified into nine serotypes on the basis of hemagglutination inhibition (HI) and neuraminidase inhibition (NI) assays, although some serologic cross-reaction exists. Newcastle disease virus (NDV), which constit...


    pub_type: 杂志文章


    authors: Nayak B,Dias FM,Kumar S,Paldurai A,Collins PL,Samal SK

    更新日期:2012-03-09 00:00:00

  • Cross-protection against H7N9 influenza strains using a live-attenuated H7N3 virus vaccine.

    abstract::In 2013, avian H7N9 influenza viruses were detected infecting people in China resulting in high mortality. Influenza H7 vaccines that provide cross-protection against these new viruses are needed until specific H7N9 vaccines are ready to market. In this study, an available H7N3 cold-adapted, temperature sensitive, liv...


    pub_type: 杂志文章


    authors: Carter DM,Bloom CE,Kirchenbaum GA,Tsvetnitsky V,Isakova-Sivak I,Rudenko L,Ross TM

    更新日期:2015-01-01 00:00:00

  • The role of the vaccines industry in Mission Grand Convergence.

    abstract::The vast majority of vaccines used throughout the world are supplied by the private sector. It is essential therefore that the industry is closely engaged in future policy developments at a national and international level and is able to respond to the changing needs and priorities that may be required to ensure the s...


    pub_type: 杂志文章


    authors: Almond J,Medaglini D

    更新日期:2017-01-20 00:00:00

  • A therapeutic HIV-1 vaccine enhances anti-HIV-1 immune responses in patients under highly active antiretroviral therapy.

    abstract:BACKGROUND:HIV-1 specific cellular immunity plays an important role in controlling viral replication. In this first-in-human therapeutic vaccination study, a replication-defective HIV-1 vaccine (HIVAX) was tested in HIV-1 infected participants undergoing highly active antiretroviral therapy (HAART) to enhance anti-HIV ...


    pub_type: 杂志文章,随机对照试验


    authors: Tung FY,Tung JK,Pallikkuth S,Pahwa S,Fischl MA

    更新日期:2016-04-27 00:00:00

  • Generation of recombinant pandemic H1N1 influenza virus with the HA cleavable by bromelain and identification of the residues influencing HA bromelain cleavage.

    abstract::The proteolytic enzyme bromelain has been traditionally used to cleave the hemagglutinin (HA) protein at the C-terminus of the HA2 region to release the HA proteins from influenza virions. The bromelain cleaved HA (BHA) has been routinely used as an antigen to generate antiserum that is essential for influenza vaccine...


    pub_type: 杂志文章


    authors: Wang W,Suguitan AL Jr,Zengel J,Chen Z,Jin H

    更新日期:2012-01-20 00:00:00

  • Case-based surveillance enhanced with measles virus detection/genotyping is essential to maintain measles elimination in Aichi Prefecture, Japan.

    abstract:BACKGROUND:Japan was verified as having achieved measles elimination by the Measles Regional Verification Commission in the Western Pacific Region in March 2015. Verification of measles elimination implies the absence of continuous endemic transmission. After the last epidemic in 2007 with an estimated 18,000 cases, Ja...


    pub_type: 杂志文章


    authors: Minagawa H,Yasui Y,Adachi H,Ito M,Hirose E,Nakamura N,Hata M,Kobayashi S,Yamashita T

    更新日期:2015-11-09 00:00:00

  • The virulence-related rhoptry protein 5 (ROP5) of Toxoplasma Gondii is a novel vaccine candidate against toxoplasmosis in mice.

    abstract::Infections with the intracellular protozoan parasite Toxoplasma gondii pose a serious public health problem and are of great economic importance worldwide. The parasite rhoptry protein 5 (ROP5) has been implicated as a major virulence factor that reduces the accumulation of immunity-related GTPases (IRG) in parasitoph...


    pub_type: 杂志文章


    authors: Zheng B,Lu S,Tong Q,Kong Q,Lou D

    更新日期:2013-09-23 00:00:00

  • TLR-based immune adjuvants.

    abstract::This work describes the nature and strength of the immune response induced by various Toll-like receptor ligands and their ability to act as vaccine adjuvants. It reviews the various ligands capable of triggering individual TLRs, and then focuses on the efficacy and safety of those agents for which clinical results ar...


    pub_type: 杂志文章,评审


    authors: Steinhagen F,Kinjo T,Bode C,Klinman DM

    更新日期:2011-04-12 00:00:00

  • Very small size proteoliposomes derived from Neisseria meningitidis: An effective adjuvant for generation of CTL responses to peptide and protein antigens.

    abstract::The development of potent adjuvants, conditioning innate and adaptative immunity, particularly CTL responses, has become currently a hot point in the rational design of vaccines for cancer immunotherapy. We have described a new approach, in which gangliosides are incorporated into vesicles from Neisseria meningitidis ...


    pub_type: 杂志文章


    authors: Mesa C,de León J,Fernández LE

    更新日期:2006-03-24 00:00:00

  • Tracking parental attitudes on vaccination across European countries: The Vaccine Safety, Attitudes, Training and Communication Project (VACSATC).

    abstract::The paper presents the first results from the European project VACSATC which aimed to track parental attitudes on vaccinations across several European countries. We compared five cross-sectional surveys of parents with children less than 3 years of age in England, Norway, Poland, Spain and Sweden carried out during 20...


    pub_type: 杂志文章


    authors: Stefanoff P,Mamelund SE,Robinson M,Netterlid E,Tuells J,Bergsaker MA,Heijbel H,Yarwood J,VACSATC working group on standardization of attitudinal studies in Europe.

    更新日期:2010-08-09 00:00:00

  • Mapping financial flows for immunisation in Uganda 2009/10 and 2010/11: New insights for methodologies and policy.

    abstract:BACKGROUND:The Global Vaccine Action Plan highlights the need for immunisation programmes to have sustainable access to predictable funding. A good understanding of current and future funding needs, commitments, and gaps is required to enhance planning, improve resource allocation and mobilisation, and to avoid funding...


    pub_type: 杂志文章


    authors: Guthrie T,Zikusooka C,Kwesiga B,Abewe C,Lagony S,Schutte C,Marinda E,Humphreys K,Motlogelwa K,Nombewu ZC,Brenzel L,Kinghorn A

    更新日期:2015-05-07 00:00:00

  • Vaccination against leprosy at Ben San Leprosy Centre, Ho Chi Minh City, Vietnam.

    abstract::Three vaccines, BCG alone, BCG + 10(7) killed Mycobacterium vaccae and 10(8) killed M. vaccae alone, were studied in children living in close contact with leprosy. In the year before vaccination, 14/446 (3.1%) children had developed leprosy. Among those who were not vaccinated, 9/74 (12.2%) developed the disease in th...


    pub_type: 杂志文章


    authors: Truoc LV,Ly HM,Thuy NK,Trach DD,Stanford CA,Stanford JL

    更新日期:2001-05-14 00:00:00

  • Effect of passive antibody on the immune response of cotton rats to purified F and G glycoproteins of respiratory syncytial virus (RSV).

    abstract::The effect of passively transferred RSV immune serum on the antibody response to a single dose of purified RSV fusion (F) and large (G) glycoproteins was studied in cotton rats. Passively transferred antibody that achieved serum antibody levels similar to those seen in newborn human infants resulted in a seven- to eig...


    pub_type: 杂志文章


    authors: Murphy BR,Prince GA,Collins PL,Hildreth SW,Paradiso PR

    更新日期:1991-03-01 00:00:00

  • Global Vaccine and Immunization Research Forum: Opportunities and challenges in vaccine discovery, development, and delivery.

    abstract::The World Health Organization, the National Institute of Allergy and Infectious Diseases, part of the National Institutes of Health, and the Bill & Melinda Gates Foundation convened the first Global Vaccine and Immunization Research Forum (GVIRF) in March 2014. This first GVIRF aimed to track recent progress of the Gl...


    pub_type: 杂志文章


    authors: Ford AQ,Touchette N,Hall BF,Hwang A,Hombach J

    更新日期:2016-03-18 00:00:00

  • Impact of pneumococcal conjugate vaccine in children morbidity and mortality in Peru: Time series analyses.

    abstract::Streptococcus pneumoniae is the leading cause of bacterial pneumonia, meningitis and sepsis in children worldwide. Despite available evidence on pneumococcal conjugate vaccine (PCV) impact on pneumonia hospitalizations in children, studies demonstrating PCV impact in morbidity and mortality in middle-income countries ...


    pub_type: 杂志文章


    authors: Suarez V,Michel F,Toscano CM,Bierrenbach AL,Gonzales M,Alencar AP,Ruiz Matus C,Andrus JK,de Oliveira LH

    更新日期:2016-09-07 00:00:00

  • Repeated dose intramuscular injection of the CIMAvax-EGF vaccine in Sprague Dawley rats induces local and systemic toxicity.

    abstract::CIMAvax-EGF consists of a human recombinant epidermal growth factor (EGF), coupled to P64k, a recombinant carrier protein from N. meningitis, and Montanide ISA 51 as adjuvant. The vaccine immunization induces a specific antibody production, inhibiting the EGF/EGF-R interaction through EGF deprivation. The objective of...


    pub_type: 杂志文章


    authors: Mancebo A,Casacó A,González B,Ledón N,Sorlozabal J,León A,Gómez D,González Y,Bada AM,González C,Arteaga ME,Ramírez H,Fuentes D

    更新日期:2012-05-09 00:00:00

  • Improving iSC performance through outsourcing - Considerations for using third-party service providers to increase innovation, capacity and efficiency.

    abstract::Development partners and donors have encouraged and incentivized governments in developing countries to explore ways of working with third-party service suppliers to reduce costs and increase service delivery capacity. The distribution of vaccines and medicines has for a long time shown demand for outsourcing but publ...


    pub_type: 杂志文章


    authors: Wright M,Forster G,Beale J

    更新日期:2017-04-19 00:00:00

  • Correlation of haemagglutinin-neuraminidase and fusion protein content with protective antibody response after immunisation with inactivated Newcastle disease vaccines.

    abstract::The correlation between the antigen content of inactivated Newcastle disease (ND) oil emulsion-vaccines and the serological response after immunisation was studied. The haemagglutinin-neuraminidase (HN) and fusion (F) proteins of Newcastle disease virus (NDV) were quantified in 33 inactivated oil-adjuvanted ND vaccine...


    pub_type: 杂志文章


    authors: Maas RA,Komen M,van Diepen M,Oei HL,Claassen IJ

    更新日期:2003-07-04 00:00:00

  • Successful DNA immunisation of rats against fasciolosis.

    abstract::The liver fluke Fasciola hepatica contributes to great economic and health losses in the cattle industry in many countries, including Poland. Unfortunately, no vaccine against fasciolosis is commercially available. We have designed a DNA vaccine and tested it in rats. Groups of male or female rats received one intramu...


    pub_type: 杂志文章


    authors: Kofta W,Mieszczanek J,Płucienniczak G,Wedrychowicz H

    更新日期:2000-07-01 00:00:00

  • The science of vaccine safety: Summary of meeting at Wellcome Trust.

    abstract::Vaccines are everywhere hugely successful but are also under attack. The reason for the latter is the perception by some people that vaccines are unsafe. However that may be, vaccine safety, life any other scientific subject, must be constantly studied. It was from this point of view that a meeting was organized at th...


    pub_type: 杂志文章


    authors: Plotkin SA,Offit PA,DeStefano F,Larson HJ,Arora NK,Zuber PLF,Fombonne E,Sejvar J,Lambert PH,Hviid A,Halsey N,Garçon N,Peden K,Pollard AJ,Markowitz LE,Glanz J

    更新日期:2020-02-18 00:00:00

  • A national examination of pharmacy-based immunization statutes and their association with influenza vaccinations and preventive health.

    abstract:BACKGROUND:A series of state-level statute changes have allowed pharmacists to provide influenza vaccinations in community pharmacies. The study aim was to estimate the effects of pharmacy-based immunization statutes changes on per capita influenza vaccine prescriptions, adult vaccination rates, and the utilization of ...


    pub_type: 杂志文章


    authors: McConeghy KW,Wing C

    更新日期:2016-06-24 00:00:00

  • Safety, immunogenicity, and kinetics of the immune response to a single dose of virosome-formulated hepatitis A vaccine in Thais.

    abstract::The safety and immunogenicity of a single dose of virosome-formulated hepatitis A vaccine was evaluated in healthy seronegative Thai volunteers. Immunization elicited primarily mild transient reactions which did not interfere with normal activities. All subjects possessed > 20 mIU of anti-hepatitis A virus antibody pe...


    pub_type: 杂志文章


    authors: Poovorawan Y,Theamboonlers A,Chumdermpadetsuk S,Glück R,Cryz SJ Jr

    更新日期:1995-07-01 00:00:00

  • The influence of gamma inulin and Algammulin on the immune response in sheep to a recombinant antigen of Taenia ovis.

    abstract::Gamma inulin and Algammulin, two new adjuvants, were examined and compared with alum and Freund's Complete/Incomplete Adjuvant (FCA/FIA), for potentiation of cell-mediated immunity (CMI) and humoral immunity in sheep to a recombinant Taenia ovis antigen. The ability to protect sheep when challenged with live T. ovis e...


    pub_type: 杂志文章


    authors: Deol HS,Palmer DG,Dunsmore T,Carnegie PR

    更新日期:1995-04-01 00:00:00

  • Evaluation of the adequacy of measles laboratory diagnostic tests in the era of accelerating measles elimination in Beijing, China.

    abstract:BACKGROUND:Measles-containing vaccine (MCV) was introduced in 1965 in Beijing and given to the children aged 8m-14y. In population-based surveillance system, real-time polymerase chain reaction (RT-PCR) and immunoglobulin M(IgM) serology tests have been conducted for each suspected case since 2013. We used the surveill...


    pub_type: 杂志文章


    authors: Ma R,Lu L,Suo L,Zhangzhu J,Chen M,Pang X

    更新日期:2019-06-27 00:00:00

  • Efficacy of pneumococcal polysaccharide vaccine in immunocompetent adults: a meta-analysis of randomized trials.

    abstract::The use of pneumococcal polysaccharide vaccine (PPV) is low in some countries, maybe because of doubts regarding its efficacy. This meta-analysis aims at combining evidence from randomized trials of PPV assessing its efficacy in preventing Streptococcus pneumoniae related diseases in immunocompetent adults. In the fou...


    pub_type: 杂志文章,meta分析


    authors: Cornu C,Yzèbe D,Léophonte P,Gaillat J,Boissel JP,Cucherat M

    更新日期:2001-09-14 00:00:00

  • Timeliness and risk factors associated with delay for pneumococcal conjugate 10-valent routine immunization in Brazilian children.

    abstract:BACKGROUND:Vaccination coverage is the usual metrics to evaluate the immunization programs performance. For the 10-valent pneumococcal conjugate (PCV10) vaccine, measuring the delay of vaccination is also important, particularly as younger children are at increased risk of disease. Routinely collected administrative da...


    pub_type: 杂志文章


    authors: Sartori AL,Minamisava R,Afonso ET,Policena GM,Pessoni GC,Bierrenbach AL,Andrade AL

    更新日期:2017-02-15 00:00:00

  • Overcoming barriers to HPV vaccination: non-inferiority of antibody response to human papillomavirus 16/18 vaccine in adolescents vaccinated with a two-dose vs. a three-dose schedule at 21 months.

    abstract::For middle and low-income countries, the cost of HPV vaccines remains challenging. We conducted an open-label nonrandomized clinical trial evaluating immune response to the HPV-16/18 AS04-adjuvanted vaccine administered on a standard (months (M) 0-1-6) versus extended schedule (M 0-6-60) at 7, 21, 60, 72 and 120 month...


    pub_type: 临床试验,杂志文章


    authors: Lazcano-Ponce E,Stanley M,Muñoz N,Torres L,Cruz-Valdez A,Salmerón J,Rojas R,Herrero R,Hernández-Ávila M

    更新日期:2014-02-03 00:00:00

  • The induction of systemic and mucosal immune responses following the subcutaneous immunization of mature adult mice: characterization of the antibodies in mucosal secretions of animals immunized with antigen formulations containing a vitamin D3 adjuvant.

    abstract::Systemic and mucosal immune responses were effectively induced following the subcutaneous administration of Haemophilus influenzae type b oligosaccharide conjugated to diphtheria toxoid vaccine in a formulation containing the active form of vitamin D3. IgA and IgG antibodies with specificity for both the protein and o...


    pub_type: 杂志文章


    authors: Enioutina EY,Visic D,McGee ZA,Daynes RA

    更新日期:1999-08-06 00:00:00

  • Single and booster dose responses to an inactivated hepatitis A virus vaccine: comparison with immune serum globulin prophylaxis.

    abstract::Pre- and postexposure prophylaxis against hepatitis A virus (HAV) infection with immune serum globulin (Ig) is only effective for 4-6 months. We compared the safety, tolerability and immunogenicity of a single i.m. injection of Ig with a single and booster dose of an inactivated hepatitis A virus vaccine (iHAV) in adu...


    pub_type: 临床试验,杂志文章


    authors: Shouval D,Ashur Y,Adler R,Lewis JA,Armstrong ME,Davide JP,McGuire B,Kuter B,Brown L,Miller W

    更新日期:1993-01-01 00:00:00