Hepatitis C virus soluble E2 in combination with QuilA and CpG ODN induces neutralizing antibodies in mice.


:Several studies have emphasized the importance of an early, highly neutralizing antibody response in the clearance of Hepatitis C virus (HCV) infection. The envelope glycoprotein E2 is a major target for HCV neutralizing antibodies. Here, we compared antibody responses in mice immunized with native soluble E2 (sE2) from the H77 1a isolate coupled with different adjuvants or combinations of adjuvants. Adjuvanting sE2 with Freund's, monophosphoryl lipid A (MPL), cytosine phosphorothioate guanine oligodeoxynucleotide (CpG ODN), or alpha-galactosylceramide (αGalCer) derivatives elicited only moderate antibody responses. In contrast, immunizations with sE2 and QuilA elicited exceptionally high anti-E2 antibody titers. Sera from these mice effectively neutralized HCV pseudoparticles (HCVpp) 1a entry. Moreover, the combination of QuilA and CpG ODN further enhanced neutralizing antibody titers wherein cross-neutralization of HCVpp 4 was observed. We conclude that the combination of QuilA and CpG ODN is a promising adjuvant combination that should be further explored for the development of an HCV subunit vaccine. Our work also emphasizes that the ideal combination of adjuvant and immunogen has to be determined empirically.






Naarding MA,Falkowska E,Xiao H,Dragic T




Has Abstract


2011-04-05 00:00:00
















  • Reducing the loss of vaccines from accidental freezing in the cold chain: the experience of continuous temperature monitoring in Tunisia.

    abstract::Accidental freezing of vaccines is a growing threat and a real risk for national immunization programs when the potency of many vaccines can be compromised if these are exposed to sub-zero temperatures in the cold chain. In Tunisia, this issue is compounded by using sub-standard domestic cold chain equipment instead o...


    pub_type: 杂志文章


    authors: Lloyd J,Lydon P,Ouhichi R,Zaffran M

    更新日期:2015-02-11 00:00:00

  • Autovaccination of dairy cows to treat post partum metritis caused by Actinomyces pyogenes.

    abstract::Autovaccines are therapeutic vaccines manufactured from a disease causing micro-organism for individual treatment of patients, animals, or sometimes herds to treat chronic or recurrent infections. Despite the common use of autovaccines in veterinary medicine, their mechanism of action, i.e. the immunologic effector me...


    pub_type: 杂志文章


    authors: Nolte O,Morscher J,Weiss HE,Sonntag H

    更新日期:2001-04-30 00:00:00

  • Assessing the cost-effectiveness of different measles vaccination strategies for children in the Democratic Republic of Congo.

    abstract:INTRODUCTION:One of the goals of the Global Measles and Rubella Strategic Plan is the reduction in global measles mortality, with high measles vaccination coverage as one of its core components. While measles mortality has been reduced more than 79%, the disease remains a major cause of childhood vaccine preventable di...


    pub_type: 杂志文章


    authors: Doshi RH,Eckhoff P,Cheng A,Hoff NA,Mukadi P,Shidi C,Gerber S,Wemakoy EO,Muyembe-Tafum JJ,Kominski GF,Rimoin AW

    更新日期:2017-10-27 00:00:00

  • Efficacy, safety and immunogenicity of a pneumococcal protein-based vaccine co-administered with 13-valent pneumococcal conjugate vaccine against acute otitis media in young children: A phase IIb randomized study.

    abstract:BACKGROUND:Native American populations experience a substantial burden of pneumococcal disease despite use of highly effective pneumococcal conjugate vaccines (PCVs). Protein-based pneumococcal vaccines may extend protection beyond the serotype-specific protection elicited by PCVs. METHODS:In this phase IIb, double-bl...


    pub_type: 杂志文章,随机对照试验


    authors: Hammitt LL,Campbell JC,Borys D,Weatherholtz RC,Reid R,Goklish N,Moulton LH,Traskine M,Song Y,Swinnen K,Santosham M,O'Brien KL

    更新日期:2019-12-03 00:00:00

  • Immunity and protection by adoptive transfer of dendritic cells transfected with hepatitis C NS3/4A mRNA.

    abstract::In this study, we tested the hypothesis that adoptive transfer of dendritic cells (DCs) transfected ex vivo with mRNA encoding hepatitis C virus (HCV) NS3/4A would initiate potent HCV-specific protective immune responses in vivo. Murine DCs were transfected with NS3/4A mRNA or eGFP mRNA using either electroporation or...


    pub_type: 杂志文章


    authors: Yu H,Babiuk LA,van Drunen Littel-van den Hurk S

    更新日期:2007-02-26 00:00:00

  • An efficacy trial of the malaria vaccine SPf66 in Gambian infants--second year of follow-up.

    abstract::In 1994, 630 Gambian infants were immunized with three doses of the synthetic polypeptide malaria vaccine SPf66 or with a control vaccine. No significant protection against first or total attacks of malaria was observed among the children who received SPf66. However, the period of follow-up was short. Thus, 532 childr...


    pub_type: 临床试验,杂志文章


    authors: Bojang KA,Obaro SK,D'Alessandro U,Bennett S,Langerock P,Targett GA,Greenwood BM

    更新日期:1998-01-01 00:00:00

  • Impact of quadrivalent HPV vaccine dose spacing on immunologic response in women living with HIV.

    abstract::HPV vaccination schedules have changed as evidence has supported reduced dosing and extended intervals. Women living with HIV (WLWH) represent an important population with no data on alternative dosing. Girls and WLWH received quadrivalent HPV (qHPV) vaccine in a pan-Canadian study of immunogenicity and efficacy. Sero...


    pub_type: 杂志文章


    authors: McClymont E,Ogilvie G,Albert A,Johnston A,Raboud J,Walmsley S,Lipsky N,Loutfy M,Trottier S,Smaill F,Yudin MH,Klein MB,Harris M,Wobeser W,Bitnun A,Kakkar F,Samson L,Brophy J,Karatzios C,Money D,CTN 236 HPV in HIV S

    更新日期:2020-03-30 00:00:00

  • Carrier-dependent specificity of antibodies to a conserved peptide determinant of gp120.

    abstract::Amino acid residues 421-436 constitute a comparatively conserved determinant of gp120 that participates in the binding of host cell CD4 receptors by HIV-1. We compared the immunogenicity of synthetic Cys-gp120 (421-436) conjugated to KLH via the N terminal Cys residue (KLH-I) and gp120 (421-436) extended at its N term...


    pub_type: 杂志文章


    authors: Karle S,Nishiyama Y,Taguchi H,Zhou YX,Luo J,Planque S,Hanson C,Paul S

    更新日期:2003-03-07 00:00:00

  • Human rotavirus genotypes in hospitalized children, South Korea, April 2005 to March 2007.

    abstract::Availability of new rotavirus vaccines highlights the need to maintain and enhance rotavirus strain surveillance. We collected stool samples from children with gastroenteritis admitted to eight hospitals in South Korea from April 2005 to March 2007. Of the 6057 samples collected, 1337 (22%) were positive for rotavirus...


    pub_type: 杂志文章


    authors: Lee SY,Hong SK,Lee SG,Suh CI,Park SW,Lee JH,Kim JH,Kim DS,Kim HM,Jang YT,Ma SH,Kim SY,Sohn YS,Kang JH,Paik SY

    更新日期:2009-11-20 00:00:00

  • Epidemiology of hepatitis B infection in North America.

    abstract::Although the USA is considered an area of 'low' endemicity for hepatitis B infection, the incidence of new cases, the prevalence of carriers, and the burden of acute and chronic disease place hepatitis B among the most important communicable diseases. It is estimated that 300,000 new cases of hepatitis B infection occ...


    pub_type: 杂志文章


    authors: Kane M

    更新日期:1995-01-01 00:00:00

  • Cross-protection against H7N9 influenza strains using a live-attenuated H7N3 virus vaccine.

    abstract::In 2013, avian H7N9 influenza viruses were detected infecting people in China resulting in high mortality. Influenza H7 vaccines that provide cross-protection against these new viruses are needed until specific H7N9 vaccines are ready to market. In this study, an available H7N3 cold-adapted, temperature sensitive, liv...


    pub_type: 杂志文章


    authors: Carter DM,Bloom CE,Kirchenbaum GA,Tsvetnitsky V,Isakova-Sivak I,Rudenko L,Ross TM

    更新日期:2015-01-01 00:00:00

  • Immunologic studies of specific mucosal and systemic immune responses in Mexican school children after booster aerosol or subcutaneous immunization with measles vaccine.

    abstract::The purpose of the present study was to compare serum and mucosal immune responses following either aerosol (Aer) or subcutaneous (SQ) measles immunization of Mexican school children. A cohort of 49 children from 6 to 7 years of age received either Aer ( n = 22) or SQ ( n = 27) Edmonston-Zagreb (EZ) measles vaccine. S...


    pub_type: 杂志文章


    authors: Bellanti JA,Zeligs BJ,Mendez-Inocencio J,García-Garcia ML,Islas-Romero R,Omidvar B,Omidvar J,Kim G,Fernandez De Castro J,Sepulveda Amor J,Walls L,Bellini WJ,Valdespino-Gomez JL

    更新日期:2004-03-12 00:00:00

  • Evaluation and validation of a serum bactericidal antibody assay for Haemophilus influenzae type b and the threshold of protection.

    abstract::Prior to routine immunisation, Haemophilus influenzae serotype b (Hib) was a major cause of serious bacterial infections, particularly in young children. In the United Kingdom, introduction of the Hib conjugate vaccine into the national childhood immunisation schedule has led to a sustained decline in invasive Hib dis...


    pub_type: 杂志文章


    authors: Townsend K,Ladhani SN,Findlow H,Borrow R

    更新日期:2014-09-29 00:00:00

  • Early detection and visualization of human adenovirus serotype 5-viral vectors carrying foot-and-mouth disease virus or luciferase transgenes in cell lines and bovine tissues.

    abstract::Recombinant replication-defective human adenovirus type 5 (Ad5) vaccines containing capsid-coding regions from foot-and-mouth disease virus (FMDV) have been demonstrated to induce effective immune responses and provide homologous protective immunity against FMDV in cattle. However, basic mechanisms of Ad5-FMDV vaccine...


    pub_type: 杂志文章


    authors: Montiel N,Smoliga G,Arzt J

    更新日期:2012-02-21 00:00:00

  • Expected epidemiological impact of the introduction of a partially effective HIV vaccine among men who have sex with men in Australia.

    abstract::A trial of the ALVAC-AIDSVAX HIV vaccine was recently found to be partially effective in preventing HIV transmission among study participants in Thailand. The success of this trial means that vaccination may become a viable intervention for the prevention of HIV infection in the medium-term future. Assuming that the v...


    pub_type: 杂志文章


    authors: Gray RT,Ghaus MH,Hoare A,Wilson DP

    更新日期:2011-08-18 00:00:00

  • Guidance on the evaluation of Plasmodium vivax vaccines in populations exposed to natural infection.

    abstract::In this paper we give guidance for the design and conduct of vaccine trials against Plasmodium vivax malaria. The paper supplements earlier guidelines on the planning of vaccine trials against Plasmodium falciparum malaria [WHO. Guidelines for the evaluation of Plasmodium falciparum vaccines in populations exposed to ...


    pub_type: 杂志文章,实务指引,评审


    authors: Mueller I,Moorthy VS,Brown GV,Smith PG,Alonso P,Genton B,WHO Malaria Vaccine Advisory Committee (MALVAC).

    更新日期:2009-09-18 00:00:00

  • HBV vaccination in hyperendemic remote tribal areas in India.

    abstract::Randomly selected 200 subjects were tested for quantitative hepatitis B surface antibody (HBsAb) estimation in a remote tribal area of Arunachal Pradesh, India to measure the protective immunity after vaccination in the backdrop of hyperendemic HBV infection. About 64.6 and 40.0% had HBsAb above 10 mIU/ml in the vacci...


    pub_type: 信件


    authors: Borkakoty B,Mahanta J,Biswas D

    更新日期:2007-12-05 00:00:00

  • Antibodies against Haemophilus influenzae type b in The Gambia: investigating the extent of protection across age groups.

    abstract::Following a landmark clinical trial, the vaccine against Haemophilus influenzae type b (Hib) was introduced in The Gambia in 1997. Whilst the immunogenicity of this vaccine is well established subsequent to the doses administered under the EPI schedule, little data exists assessing longevity of protection, using serol...


    pub_type: 杂志文章


    authors: Idoko OT,Roberts E,Cox M,Jafali J,Njie-Jobe J,Mackenzie G,Ota MO,Kampmann B

    更新日期:2014-08-06 00:00:00

  • Immunization of pregnant women: reproductive, medical and societal risks.

    abstract::Establishing successful vaccine programs for pregnant women would be the quintessence of preventive medicine when you realize the preventive potential for reproductive problems of many of the new and old vaccines. The development of vaccines to prevent maternal, fetal and newborn disease is actually in its infancy. Th...


    pub_type: 杂志文章,评审


    authors: Brent RL

    更新日期:2003-07-28 00:00:00

  • Seizing market shaping opportunities for vaccine cold chain equipment.

    abstract::Gavi, the Vaccine Alliance, supports immunisation programmes in eligible countries to reach children with lifesaving vaccines. Dramatic improvement in the scale and performance of current cold chain systems is required to extend the reach of immunisation services - especially for children living in remote locations - ...


    pub_type: 杂志文章,评审


    authors: Azimi T,Franzel L,Probst N

    更新日期:2017-04-19 00:00:00

  • Vaccination of sheep against M. paratuberculosis: immune parameters and protective efficacy.

    abstract::Johne's disease in ruminants is caused by the pathogenic bacterium Mycobacterium avium subspecies paratuberculosis (Map). Currently available Map commercial vaccines protect against clinical disease but not infection. In this study, the proprietary Johne's vaccine Neoparasec and an aqueous formulation of Map 316F (Aqu...


    pub_type: 临床试验,杂志文章


    authors: Begg DJ,Griffin JF

    更新日期:2005-10-10 00:00:00

  • DNA vaccination of poultry: The current status in 2015.

    abstract::DNA vaccination is a promising alternative strategy for developing new human and animal vaccines. The massive efforts made these past 25 years to increase the immunizing potential of this kind of vaccine are still ongoing. A relatively small number of studies concerning poultry have been published. Even though there i...


    pub_type: 杂志文章,评审


    authors: Meunier M,Chemaly M,Dory D

    更新日期:2016-01-04 00:00:00

  • The use of sequence analysis of a feline calicivirus (FCV) hypervariable region in the epidemiological investigation of FCV related disease and vaccine failures.

    abstract::A reverse transcriptase polymerase chain reaction (PCR) was used to amplify a 235 bp hypervariable region of the feline calicivirus (FCV) genome which encodes part of the capsid protein. Sequence from this region was used to compare viruses used in three attenuated vaccines to viruses isolated from vaccinated cats wit...


    pub_type: 杂志文章


    authors: Radford AD,Bennett M,McArdle F,Dawson S,Turner PC,Glenn MA,Gaskell RM

    更新日期:1997-08-01 00:00:00

  • Immunotherapy with combined DNA vaccines is an effective treatment for M. bovis infection in cattle.

    abstract::Mycobacterium bovis causes tuberculosis in cattle. Because current therapies inadequately prevent disease spread, new therapies for M. bovis infections are urgently needed. We treated M. bovis-infected cattle with combinations of DNA vaccines, resulting in significantly reduced pulmonary bacterial loads compared with ...


    pub_type: 杂志文章


    authors: Hu XD,Chen ST,Yu DH,Li SX,Cai H

    更新日期:2009-02-25 00:00:00

  • Cost-effectiveness of vaccination of the elderly against herpes zoster in The Netherlands.

    abstract:BACKGROUND:Each year a substantial number of Dutch elderly suffers from herpes zoster (HZ), caused by the reactivation of the varicella zoster virus (VZV). A potential complication of HZ is postherpetic neuralgia (PHN) which results in a prolonged loss of quality of life. A large randomized clinical trial, labelled the...


    pub_type: 杂志文章


    authors: de Boer PT,Pouwels KB,Cox JM,Hak E,Wilschut JC,Postma MJ

    更新日期:2013-02-18 00:00:00

  • Going social: Success in online recruitment of men who have sex with men for prevention HIV vaccine research.

    abstract:OBJECTIVE:To compare the use of four different social media sites to recruit men who have sex with men (MSM) and transgender women to a phase 2b HIV prevention vaccine trial, HVTN 505. DESIGN:Retrospective, observational study. METHODS:The University of Pennsylvania HIV Vaccine Trials Unit (Penn HVTU) employed street...


    pub_type: 杂志文章


    authors: Buckingham L,Becher J,Voytek CD,Fiore D,Dunbar D,Davis-Vogel A,Metzger DS,Frank I

    更新日期:2017-06-14 00:00:00

  • Relating cognitive function to military aviator performance in early HIV infection.

    abstract::There has been controversy about whether cognitive changes occur in early human immunodeficiency virus (HIV) disease. In those studies reporting cognitive changes, these are typically subclinical, and their relationship to daily and/or occupational functioning has not been addressed. The potential effects of changes m...


    pub_type: 杂志文章


    authors: Mapou RL,Rundell JR,Kay GG,Tramont EC

    更新日期:1993-01-01 00:00:00

  • Strategic health communication on social media: Insights from a Danish social media campaign to address HPV vaccination hesitancy.

    abstract:OBJECTIVE:To evaluate whether the social media strategy developed for the campaign Stop HPV - stop cervical cancer was successful at engaging target groups in communication regarding HPV vaccination. INTRODUCTION:In 2009, the Human Papillomavirus (HPV) vaccine became part of the Danish childhood vaccination program to...


    pub_type: 杂志文章


    authors: Pedersen EA,Loft LH,Jacobsen SU,Søborg B,Bigaard J

    更新日期:2020-06-26 00:00:00

  • Protection against experimental bubonic and pneumonic plague by a recombinant capsular F1-V antigen fusion protein vaccine.

    abstract::The current human whole-cell vaccine is ineffective against pneumonic plague caused by typical F1 capsule positive (F1+) strains of Yersinia pestis. The authors found this vaccine to also be ineffective against F1-negative (F1-) Y. pestis strains, which have been isolated from a human case and from rodents. For these ...


    pub_type: 杂志文章


    authors: Heath DG,Anderson GW Jr,Mauro JM,Welkos SL,Andrews GP,Adamovicz J,Friedlander AM

    更新日期:1998-07-01 00:00:00

  • Prevalence of human papillomavirus types in cervical and oral cancers in central India.

    abstract::Cervical cancer that is associated with high-risk human papillomaviruses (HPV) is the most common malignancy in Indian women. Therefore, the establishment of a prevention program is urgently required considering both vaccination and screening. However, relatively little is known about the prevalence of the different H...


    pub_type: 杂志文章


    authors: Gheit T,Vaccarella S,Schmitt M,Pawlita M,Franceschi S,Sankaranarayanan R,Sylla BS,Tommasino M,Gangane N

    更新日期:2009-01-29 00:00:00