Development of a candidate reference material for adventitious virus detection in vaccine and biologicals manufacturing by deep sequencing.

abstract：:Optimized DNA vectors were constructed comprising the proteome of SIV including the structural, enzymatic, regulatory, and accessory proteins. In addition to native antigens as produced by the virus, fusion proteins and modified antigens with altered secretion, cellular localization and stability characteristics were ...

journal_title：Vaccine

authors： Kulkarni V,Jalah R,Ganneru B,Bergamaschi C,Alicea C,von Gegerfelt A,Patel V,Zhang GM,Chowdhury B,Broderick KE,Sardesai NY,Valentin A,Rosati M,Felber BK,Pavlakis GN

更新日期：2011-09-09 00:00:00

abstract：:Cationic antimicrobial peptides (CAMPs) are active defence components of the innate immune system. Several artificial CAMPs have been designed as antibiotic peptide therapeutics, but none have been reported to exert adjuvant activity in animal models. Here we show for the first time that an artificial CAMP, KLKLLLLLKL...

journal_title：Vaccine

authors： Fritz JH,Brunner S,Birnstiel ML,Buschle M,Gabain Av,Mattner F,Zauner W

更新日期：2004-09-03 00:00:00

abstract：:A gene encoding the mature Escherichia coli heat-labile enterotoxin B subunit (LTB) was introduced in a vector pNU212 and expressed at high levels in Bacillus brevis HPD31. The maximum amount of recombinant LTB (rLTB) secreted into the modified 5PY medium containing erythromycin was about 350 mg l(-1) when cultivated ...

journal_title：Vaccine

authors： Kozuka S,Yasuda Y,Isaka M,Masaki N,Taniguchi T,Matano K,Moriyama A,Ohkuma K,Goto N,Udaka S,Tochikubo K

更新日期：2000-03-06 00:00:00

abstract：:Persistence of antibodies following a single dose of Tdap vaccine (tetanus, diphtheria, and five-component acellular pertussis vaccine for use in individuals past childhood) was evaluated in a follow-up of adolescents (N=324) and adults (N=644) who had received Tdap in earlier clinical trials. Outcome measures were se...

journal_title：Vaccine

authors： Barreto L,Guasparini R,Meekison W,Noya F,Young L,Mills E

更新日期：2007-11-23 00:00:00

abstract：:Previous work by our laboratory identified a fraction of Schistosoma mansoni soluble adult worm antigenic preparation, designated PIII, able to elicit significant in vitro cell proliferation, and lower in vitro and in vivo granuloma formation. In the present work, we investigated some biological activities of P24, an ...

journal_title：Vaccine

authors： Zouain CS,Gustavson S,Oliveira SC,Azevedo V,Alves JB,Goes AM

更新日期：2000-12-08 00:00:00

abstract：:Pregnant goats were employed to assess unmarked deletion mutant vaccine candidates BMDeltaasp24, BMDeltacydBA, and BMDeltavirB2, as the target host species naturally infected with Brucella melitensis. Goats were assessed for the degree of pathology associated with the vaccine strains as well as the protective immunity...

journal_title：Vaccine

authors： Kahl-McDonagh MM,Elzer PH,Hagius SD,Walker JV,Perry QL,Seabury CM,den Hartigh AB,Tsolis RM,Adams LG,Davis DS,Ficht TA

更新日期：2006-06-12 00:00:00

abstract：BACKGROUND:Zika virus vaccine development is underway. We examined interest in receiving a Zika virus vaccine (after one becomes commercially available) among students at a large public university in Northern Virginia. METHODS:An online survey of Zika virus-related knowledge, attitudes, and interest in receiving a Zik...

journal_title：Vaccine

authors： Painter JE,Plaster AN,Tjersland DH,Jacobsen KH

更新日期：2017-02-07 00:00:00

abstract：:Pertussis is an acute respiratory infectious disease caused by the bacterium Bordetella pertussis. Although pertussis vaccination was introduced in the 1960s, pertussis is still an endemic disease in China. To better understand the genetic diversity of the Chinese B. pertussis population, we characterized 115 clinical...

journal_title：Vaccine

authors： Xu Y,Zhang L,Tan Y,Wang L,Zhang S,Wang J

更新日期：2015-11-17 00:00:00

abstract：BACKGROUND:The safety and tolerability of Flublok(®), a purified recombinant hemagglutinin seasonal influenza vaccine, was compared to AFLURIA(®) in a randomized, blinded clinical trial in adults ≥ 50 years of age with attention to hypersensitivity reactions. METHODS:This blinded, randomized trial of healthy adults ≥ ...

journal_title：Vaccine

authors： Izikson R,Leffell DJ,Bock SA,Patriarca PA,Post P,Dunkle LM,Cox MM

更新日期：2015-11-27 00:00:00

abstract：:Gavi, the Vaccine Alliance, supports immunisation programmes in eligible countries to reach children with lifesaving vaccines. Dramatic improvement in the scale and performance of current cold chain systems is required to extend the reach of immunisation services - especially for children living in remote locations - ...

journal_title：Vaccine

authors： Azimi T,Franzel L,Probst N

更新日期：2017-04-19 00:00:00

abstract：BACKGROUND:Chronic hepatitis C virus (HCV) and HIV infections are associated with impaired responses to neo-antigens contained in hepatitis A virus (HAV)/hepatitis B virus (HBV) vaccines, yet responsible mechanisms are unclear. METHODS:ACTG 5232 and CFAR0910 were clinical trials where pre-vaccine levels of plasma IP10...

journal_title：Vaccine

authors： Shive CL,Judge CJ,Clagett B,Kalayjian RC,Osborn M,Sherman KE,Fichtenbaum C,Gandhi RT,Kang M,Popkin DL,Sieg SF,Lederman MM,Rodriguez B,Anthony DD

更新日期：2018-01-25 00:00:00

abstract：:Dengue disease is a global challenge for healthcare systems particularly during outbreaks, and millions of dollars are spent every year for vector control. An efficient and safe vaccine that is cost-effective could resolve the burden that dengue virus imposes on affected countries. We describe here the immunogenicity ...

journal_title：Vaccine

authors： Zuest R,Valdes I,Skibinski D,Lin Y,Toh YX,Chan K,Hermida L,Connolly J,Guillen G,Fink K

更新日期：2015-03-17 00:00:00

abstract：:We previously developed a dengue tetravalent DNA vaccine that can induce neutralizing antibodies against four dengue viruses in mice. Here, we demonstrated that immunogenicity of our tetravalent vaccine is synergistically increased in mice by co-immunization with dengue type 2 virus (DENV2) subviral extracellular part...

journal_title：Vaccine

authors： Imoto J,Konishi E

更新日期：2007-01-22 00:00:00

abstract：:Mumps virus is still circulating and annual mumps outbreaks occur with fluctuating magnitudes in Japan. Aseptic meningitis has been reported after vaccination and it would be of importance to determine whether this was related to the vaccination. The objective of this study was to develop a sensitive, specific and rap...

journal_title：Vaccine

authors： Yoshida N,Fujino M,Ota Y,Notomi T,Nakayama T

更新日期：2007-01-26 00:00:00

abstract：BACKGROUND:We aim to determine the vaccination coverage of social and healthcare workers in International sites of Samusocial, providing emergency care to homeless people, and to assess factors associated with having received necessary doses at adulthood. METHODS:Data on immunization coverage of social and healthcare ...

journal_title：Vaccine

authors： Marshall E,Salmon D,Bousfiha N,Togola Y,Ouedraogo F,Santantonio M,Dieng CK,Tartière S,Emmanuelli X

更新日期：2017-09-18 00:00:00

abstract：:To circumvent limitations of poor antigen presentation and immunogenicity of DNA vaccines that target induction of CD8(+) T cell immunity, we have generated single chain MHC I trimers (MHC I SCTs) composed of a single polypeptide chain with a linear composition of antigenic peptide, β2-microglobulin, and heavy chain o...

journal_title：Vaccine

authors： Kim S,Zuiani A,Carrero JA,Hansen TH

更新日期：2012-03-09 00:00:00

abstract：:The correlation between the antigen content of inactivated Newcastle disease (ND) oil emulsion-vaccines and the serological response after immunisation was studied. The haemagglutinin-neuraminidase (HN) and fusion (F) proteins of Newcastle disease virus (NDV) were quantified in 33 inactivated oil-adjuvanted ND vaccine...

journal_title：Vaccine

authors： Maas RA,Komen M,van Diepen M,Oei HL,Claassen IJ

更新日期：2003-07-04 00:00:00

abstract：:Aluminium-containing adjuvants are often used to enhance the potency of vaccines. In the present work we studied whether adsorption of diphtheria toxoid to colloidal aluminium hydroxide induces conformational changes of the antigen. Diphtheria toxoid has a high affinity for the aluminium hydroxide particles based on a...

journal_title：Vaccine

authors： Régnier M,Metz B,Tilstra W,Hendriksen C,Jiskoot W,Norde W,Kersten G

更新日期：2012-11-06 00:00:00

abstract：:Enterotoxigenic Escherichia coli (ETEC), which produces heat labile toxin (LT) and/or heat stable toxin (ST), is considered to be the most common known cause of travellers' diarrhoea (TD). Owing to the antigenic similarity between cholera toxin and LT, immunization with inactivated oral B-subunit/whole-cell cholera va...

journal_title：Vaccine

authors： Leyten EM,Soonawala D,Schultsz C,Herzog C,Ligthelm RJ,Wijnands S,Visser LG

更新日期：2005-10-17 00:00:00

abstract：:One hundred healthy Caucasian medical students (age 22 +/- 1 years) were vaccinated with a recombinant hepatitis B vaccine and their haptoglobin types were determined. A relationship between haptoglobin type and immune response to the vaccine was observed. Subjects with a 2-2 haptoglobin phenotype produced significant...

journal_title：Vaccine

authors： Louagie H,Delanghe J,Desombere I,De Buyzere M,Hauser P,Leroux-Roels G

更新日期：1993-09-01 00:00:00

abstract：:Viral hepatitis remains a major contributor to the global disease burden. Mass immunisation strategies against hepatitis B have been adopted by more than 90 developing and industrialised countries. Countries with low hepatitis A endemicity are experiencing cyclical outbreaks and an epidemiological shift, with larger n...

journal_title：Vaccine

authors： Lopalco PL,Salleras L,Barbuti S,Germinario C,Bruguera M,Buti M,Domínguez A

更新日期：2000-10-15 00:00:00

abstract：INTRODUCTION:In recent years, vaccine preventable diseases such as measles and pertussis have been re-emerging in Western countries, maybe because of decreasing participation in childhood vaccination programs in some countries. There is clear evidence for vaccine efficacy and the risk of adverse effects is low. This ne...

journal_title：Vaccine

authors： Óskarsson Ý,Guðnason Þ,Jónsdóttir GA,Kristinsson KG,Briem H,Haraldsson Á

更新日期：2015-12-16 00:00:00

abstract：BACKGROUND:Since 2006, two rotavirus vaccines have been licensed in Taiwan, either as a 2- (RV1) or 3-dose (RV5) schedule administered at ages 2, 4, and 6 months. This study assessed the risk of intussusception and Kawasaki disease (KD) associated with rotavirus vaccines among infants. METHODS:Cases of intussusception...

journal_title：Vaccine

authors： Huang WT,Juan YC,Liu CH,Yang YY,Chan KA

更新日期：2020-09-11 00:00:00

abstract：:An effective vaccination strategy against human immunodeficiency virus type 1 (HIV-1) should include the induction of potent cellular immune responses against conserved HIV-1 antigens. We have generated five replication competent recombinant influenza viruses (rFlu/SIV Gag nos. 1-5) expressing different portions of Ga...

journal_title：Vaccine

authors： Nakaya Y,Zheng H,García-Sastre A

更新日期：2003-05-16 00:00:00

abstract：:The worldwide HIV-1 vaccine research endeavor is focused increasingly on subtype C, which is now the predominant strain of the present HIV/AIDS epidemic. Expression cassettes of HIV-1 subtype C gag, pol and versions of gagpol fusion cassettes were constructed and evaluated for their relative abilities to induce cellul...

journal_title：Vaccine

authors： Megede JZ,Otten GR,Doe B,Liu H,Srivastava R,Greer C,Legg H,Tang T,Polo JM,Donnelly JJ,Ulmer JB,Barnett SW

更新日期：2006-04-05 00:00:00

abstract：:Persistent infection by high risk genotypes of human papillomavirus (HPV) is the cause of cervical cancer, which remains one of the most common cancers among women worldwide. In addition, there is a growing appreciation that high risk HPVs are associated with a number of other cancers including anogenital cancers as w...

journal_title：Vaccine

authors： Wick DA,Webb JR

更新日期：2011-10-13 00:00:00

abstract：BACKGROUND:Females who receive the human papillomavirus (HPV) vaccine may believe they are protected from developing cervical cancer and no longer require screening. Concern has also been expressed that vaccinated females are those that would be screened regularly. This study assesses the Pap testing behavior of vaccin...

journal_title：Vaccine

authors： Kliewer EV,Mahmud SM,Demers AA,Lambert P

更新日期：2013-12-17 00:00:00

abstract：:Recombinant plasmid DNA constructs expressing truncated or full-length dengue-1 envelope (E) with or without the pre-membrane (prM) were tested for immunogenicity in mice, as candidate dengue DNA vaccines. Two plasmids, one expressing the N-terminal 80% E and the other expressing prM and full length E were immunogenic...

journal_title：Vaccine

authors： Raviprakash K,Kochel TJ,Ewing D,Simmons M,Phillips I,Hayes CG,Porter KR

更新日期：2000-05-08 00:00:00

abstract：:Data from two Swedish pertussis vaccine trials with various combination vaccines were used to compare anti-diphtheria antitoxin concentrations over time between different vaccines, vaccine lots and vaccine schedules. The immune responses were measured with a validated ELISA method.Results are given for 1326 children, ...

journal_title：Vaccine

authors： Tiru M,Hallander HO,Gustafsson L,Storsaeter J,Olin P

更新日期：2000-04-28 00:00:00

abstract：OBJECTIVE:Pneumococcal disease is a significant problem in immunocompromised persons, particularly in HIV-infected individuals. The CDC recently updated pneumococcal vaccination recommendations for immunocompromised adults, adding the 13-valent pneumococcal conjugate vaccine (PCV13) to the previously recommended 23-val...

journal_title：Vaccine

authors： Smith KJ,Nowalk MP,Raymund M,Zimmerman RK

更新日期：2013-08-20 00:00:00