Abstract：
:Aluminium-containing adjuvants are often used to enhance the potency of vaccines. In the present work we studied whether adsorption of diphtheria toxoid to colloidal aluminium hydroxide induces conformational changes of the antigen. Diphtheria toxoid has a high affinity for the aluminium hydroxide particles based on a high adsorption degree, adsorption rate and adsorptive capacity. The conformation and stability of diphtheria toxoid in solution and adsorbed to aluminium hydroxide adjuvant were characterized using five physicochemical techniques: intrinsic and extrinsic fluorescence spectroscopy, circular dichroism, infrared spectroscopy and differential scanning calorimetry. Diphtheria toxoid adsorbed to aluminium hydroxide resulted in a minimal shift of the tryptophan fluorescence spectrum, whereas a large increase in the emission of the Bis-ANS probe was observed, indicating that hydrophobic sites of the protein became accessible due to adsorption. In addition, circular dichroism and infrared spectroscopy revealed that adsorption to aluminium hydroxide caused an increase of β-sheet content and a decrease of α-helix content in diphtheria toxoid. Differential scanning calorimetry demonstrated a major decrease in the enthalpy of denaturation upon adsorption. In conclusion, the adsorption of diphtheria toxoid to aluminium hydroxide adjuvant leads to substantial conformational changes in the antigen. Since physicochemical methods can be used to monitor these conformational changes, these analytical methods might offer a tool in regulatory required vaccine quality control by demonstrating consistency in production.
journal_name
Vaccinejournal_title
Vaccineauthors
Régnier M,Metz B,Tilstra W,Hendriksen C,Jiskoot W,Norde W,Kersten Gdoi
10.1016/j.vaccine.2012.09.020subject
Has Abstractpub_date
2012-11-06 00:00:00pages
6783-8issue
48eissn
0264-410Xissn
1873-2518pii
S0264-410X(12)01330-8journal_volume
30pub_type
杂志文章相关文献
VACCINE文献大全abstract：:Age related deterioration in the function of the immune system has been recognised in many species. The clinical presentations of such immune dysfunction are an age-related increased susceptibility to certain infections, and an increased incidence of autoimmune disease and certain cancers. Laboratory investigations re...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/s0264-410x(99)00498-3
更新日期：2000-02-25 00:00:00
abstract：:A study of the serological and epidemiological effects of primary immunization with current live attenuated measles vaccine (Hu191) among 503 children aged 6-15 months, was conducted in Jingzhou, Hubei province, China from 1991 to 1998. The results showed that the positive conversion rate of measles IgG was 91.65%, ge...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0264-410x(00)00426-6
更新日期：2001-02-28 00:00:00
abstract：:The aim of this study was to estimate the effectiveness of 2009 seasonal trivalent inactivated vaccine in reducing hospitalizations due to the novel influenza A H1N1 virus among positive cases. Data collected from Argentina's national epidemiological surveillance system were analyzed. All patients had a clinical diagn...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.05.051
更新日期：2010-07-19 00:00:00
abstract：:We report the results of the handsearch of all the issues of Vaccine from 1983 to 1994 to identify randomized and controlled clinical trials (RCTs and CCTs) with the aim of setting up a Cochrane data base of vaccine trials. We read 1119 original papers and located 231 trials of which 117 (50.6%) were RCTs and 114 (49....
journal_title：Vaccine
pub_type： 杂志文章,meta分析
doi：10.1016/0264-410x(95)00247-x
更新日期：1996-04-01 00:00:00
abstract：:Buffalopox virus (BPXV), an Indian variant of vaccinia virus (VACV), is a zoonotic agent and affects buffaloes, cattle and humans. A27L is one of the conserved major immuno-dominant envelope proteins of orthopox viruses (OPVs) involved in viral entry/maturation and elicits neutralizing antibodies. In this study, the A...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.08.058
更新日期：2015-10-05 00:00:00
abstract：AIMS:The study was designed to evaluate a novel cationic lipid DNA adjuvant (N3) and its function for HIV-1gp160/rev DNA plasmid delivered intranasally. The primary N3/HIV-DNA plasmid immunizations were boosted intranasally with a gp41 peptide in a anionic L3 adjuvant. This novel prime-boost strategy of mucosal immuniz...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.08.015
更新日期：2006-05-22 00:00:00
abstract：:Severe diarrhea caused by rotavirus is a health problem worldwide, including Thailand. The World Health Organization has recommended incorporating rotavirus vaccination into national immunization programs. This policy has been implemented in several countries, but not in Thailand where the mortality rate is not high. ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.02.047
更新日期：2012-04-16 00:00:00
abstract：:Monoclonal antibody 3C9 was the starting material in the definition of the epitope that led to the synthesis of the first efficient peptide vaccine against a viral disease (canine parvovirus) in the natural host (dog). In this report, we have analysed the specificity of the antibody at the single amino acid level and ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(00)00526-0
更新日期：2001-03-21 00:00:00
abstract：BACKGROUND:Rotavirus (RV) infection is the primary cause of severe gastroenteritis in children aged <5 years in Germany and worldwide. In 2013 the German Standing Committee on Vaccination (STIKO) developed a national recommendation for routine RV-immunization of infants. To support informed decision-making we predicted...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.06.090
更新日期：2014-09-08 00:00:00
abstract：:Yellow fever (YF) is an endemic disease in some tropical areas of South America and Africa that presents lethality rate between 20 and 50%. There is no specific treatment and to control this disease a highly effective live-attenuated egg based vaccine is widely used for travelers and residents of areas where YF is end...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.02.036
更新日期：2014-05-19 00:00:00
abstract：:Hepatitis B infections are responsible for more than 300 thousand deaths per year in the Western Pacific Region. Because of this high burden, the countries and areas of the Region established a goal of reducing hepatitis B chronic infection prevalence among children to less than 1% by 2017. This study was conducted to...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2016.03.060
更新日期：2016-05-27 00:00:00
abstract：:The burden of influenza in children is increasingly appreciated; some middle-income countries are considering support for influenza vaccine programs. To support decision-making, methods to estimate the potential impact of proposed programs are needed. Using Thailand as a case-study, we present a model that uses survei...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.03.099
更新日期：2011-06-10 00:00:00
abstract：:Protective mechanisms underlying the responses to mucosal vaccination are not yet clearly defined. Using the natural mouse pneumovirus pathogen, pneumonia virus of mice (PVM), we explore responses of wild type and interferon-gamma (IFNgamma) receptor gene-deleted mice to virulent challenge after mucosal vaccination wi...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2006.09.081
更新日期：2007-01-22 00:00:00
abstract：:We demonstrated the feasibility of collecting and disseminating mid-season estimates of influenza vaccine coverage. We surveyed a nationally representative sample of U.S. adults (n = 3972) via the Internet about use of influenza vaccination as of mid-November 2008. Findings were presented on 10 December 2008 to the me...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.04.007
更新日期：2009-06-08 00:00:00
abstract：:For the production of a chemically inactivated Parapoxvirus ovis (PPVO), an adherent bovine kidney cell line was cultivated on Cytodex-3 microcarriers in suspension culture. The inactivated and purified virus particles have shown immune modulatory activity in several animal models. PPVO was produced by a biphasic batc...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.01.032
更新日期：2008-03-17 00:00:00
abstract：:Feline panleukopenia virus (FPV) is an important infectious pathogen of all members of the family Felidae. Here, we describe construction of a replication-competent recombinant canine adenovirus type 2 (CAV-2) expressing the VP2 protein of FPV (CAV-2-VP2) by transfection of MDCK cells with recombinant CAV-2 genome car...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.11.073
更新日期：2008-03-10 00:00:00
abstract：:Montanide ISA 720 is an experimental adjuvant, formulated as water-in-oil emulsions, that induces high antibody titers in several animal species. It has been used in human vaccine trials with malaria and HIV vaccines. The heightened response is likely due, in part, to the formation of a depot at the injection site. Ho...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.08.049
更新日期：2005-03-31 00:00:00
abstract：:Foot-and-mouth disease (FMD) is a highly contagious vesicular disease that affects domestic and wild cloven-hoofed animals worldwide. Recently, a series of outbreaks of type A FMDV occurred in Southeast Asian countries, China, the Russia Federation, Mongolia, Kazakhstan and South Korea. The FMD virus (A/GDMM/CHA/2013)...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.09.055
更新日期：2015-10-26 00:00:00
abstract：BACKGROUND:Implementation of 7-valent pneumococcal conjugate vaccine (PCV7) in the Dutch national immunization program for infants led to a shift from vaccine to non-vaccine serotypes in invasive pneumococcal disease (IPD) in all age groups. We studied the impact of the serotype shift on clinical syndromes and outcomes...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.12.066
更新日期：2016-02-17 00:00:00
abstract：:We successfully re-vaccinated hepatitis B virus (HBV) vaccine non-responders using a double dose of the combined hepatitis A virus (HAV) and HBV vaccine. The hope was to improve priming of hepatitis B surface antigen (HBsAg)-specific cell mediated immune response (CMI) by an increased antigen dose and a theoretical ad...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.08.054
更新日期：2008-11-05 00:00:00
abstract：:Measles incidence has decreased dramatically in China due to the implement of measles-containing vaccine (MCV). However, a measles epidemic caused resurgence recently, even among vaccinated individuals. To evaluate the effectiveness of current immunization programs and discuss initiatives for the next step in measles ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2020.03.049
更新日期：2020-05-08 00:00:00
abstract：OBJECTIVE:To determine if the addition of a physician peer educator would lead to improved immunization quality when compared to the traditional education provided as part of a vaccines for children (VFC) site visit. METHODS:A randomized controlled trial was conducted. Study participants were private pediatric and fam...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2008.05.047
更新日期：2008-08-05 00:00:00
abstract：:Two outbreaks of hepatitis A started almost simultaneously in a maternal school and in a day care centre located at opposite sides of Florence, Italy, at the end of 2002. Both of them originated from immigrant children, and in both cases, hepatitis A was initially not recognised due to aspecific symptoms. While vaccin...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.01.044
更新日期：2005-03-18 00:00:00
abstract：:In 2003, a nationwide cross-sectional study was conducted to assess the current influenza vaccination coverage of the adult population of Germany, especially in persons belonging to defined target groups. Of 666 surveyed persons, 190 (adjusted 24%) had received influenza vaccination and 419 participants belonged to at...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.12.020
更新日期：2006-03-24 00:00:00
abstract：:In the United States, co-administration of tetanus toxoid, reduced diphtheria toxoid and acellular pertussis (Tdap) vaccine and tetravalent meningococcal conjugate vaccine (MCV4) is recommended in adolescents. In this clinical study, 1341 adolescents received Tdap (Boostrix® GlaxoSmithKline) and MCV4 (Menactra®, Sanof...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2010.11.057
更新日期：2011-01-29 00:00:00
abstract：:In Portugal, the 7-valent pneumococcal conjugate vaccine (PCV7) was not introduced in the national immunization plan but was commercially available between 2001 and 2010. We studied serotype distribution and antibiotic susceptibility of Streptococcus pneumoniae carried by children in 2009 and 2010. Vaccination with PC...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2016.02.045
更新日期：2016-03-29 00:00:00
abstract：:Inactivated influenza vaccine is recommended for routine use in high-risk individuals in the United States, including women who will be in the second or third trimesters of pregnancy. The basis for this recommendation is the high risk of exposure and disease due to influenza viruses in pregnant women, as well as the i...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/s0264-410x(03)00351-7
更新日期：2003-07-28 00:00:00
abstract：:Mycoplasma hyopneumoniae, the primary pathogen of enzootic pneumonia, occurs worldwide and causes major economic losses to the pig industry. M. hyopneumoniae infects pigs at mucosal surfaces of respiratory tract. The aim of the present study was to investigate if the protection rate against M. hyopneumoniae infection ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.01.028
更新日期：2012-03-09 00:00:00
abstract：:The influenza immunization program in North America has been primarily designed to provide direct benefit to vaccinated individuals at highest risk of serious influenza outcomes. Some evidence suggests that immunization of certain age groups may also extend indirect protective benefit to vulnerable populations. Our go...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.11.090
更新日期：2008-03-04 00:00:00
abstract：:This study sought to explore how parents respond to competing media messages about vaccine safety. Six focus groups with mothers of infants were shown television vignettes of typical pro- and anti-vaccination claims. Thematic analysis of transcripts was undertaken. Mothers expressed surprise and concern about alleged ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2006.05.010
更新日期：2006-11-30 00:00:00
