Abstract：
:Optimized DNA vectors were constructed comprising the proteome of SIV including the structural, enzymatic, regulatory, and accessory proteins. In addition to native antigens as produced by the virus, fusion proteins and modified antigens with altered secretion, cellular localization and stability characteristics were generated. The DNA vectors were tested for expression upon transfection in human cells. In addition, the vectors were tested either alone or in combinations in mice and macaques, which provided an opportunity to compare immune responses in two animal models. DNA only immunization using intramuscular injection in the absence or presence of in vivo electroporation did not alter the phenotype of the induced T cell responses in mice. Although several fusion proteins induced immune responses to all the components of a polyprotein, we noted fusion proteins that abrogated immune response to some of the components. Since the expression levels of such fusion proteins were not affected, these data suggest that the immune recognition of certain components was altered by the fusion. Testing different DNA vectors in mice and macaques revealed that a combination of DNAs producing different forms of the same antigen generated more balanced immune responses, a desirable feature for an optimal AIDS vaccine.
journal_name
Vaccinejournal_title
Vaccineauthors
Kulkarni V,Jalah R,Ganneru B,Bergamaschi C,Alicea C,von Gegerfelt A,Patel V,Zhang GM,Chowdhury B,Broderick KE,Sardesai NY,Valentin A,Rosati M,Felber BK,Pavlakis GNdoi
10.1016/j.vaccine.2010.12.056subject
Has Abstractpub_date
2011-09-09 00:00:00pages
6742-54issue
39eissn
0264-410Xissn
1873-2518pii
S0264-410X(10)01829-3journal_volume
29pub_type
杂志文章相关文献
VACCINE文献大全abstract：:Japanese rabies vaccine, a purified chick embryo cell vaccine manufactured by Kaketsuken (PCEC-K), is normally given subcutaneously; however, this requires a large amount of vaccine, and the pre-exposure vaccination regimen requires 6 months to complete. These factors often hamper appropriate vaccination. Therefore, w...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.08.068
更新日期：2008-11-25 00:00:00
abstract：:There is a growing public health interest in controlling sexually transmitted infections (STIs) through vaccination due to increasing recognition of the global disease burden of STIs and the role of STIs in women's reproductive health, adverse pregnancy outcomes, and the health and well-being of neonates. Neisseria go...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2018.02.081
更新日期：2019-11-28 00:00:00
abstract：:The need for developing a case definition and guidelines for a local reaction at or near the injection site, methods for the development of the case definition and guidelines as an adverse event following immunization as well as the rationale for selected decisions about the case definition for a local reaction at or ...
journal_title：Vaccine
pub_type： 指南,杂志文章
doi：10.1016/j.vaccine.2008.10.006
更新日期：2008-12-09 00:00:00
abstract：PURPOSE:We sought to understand the relative advantage of pharmacies compared to doctors' offices for delivering HPV vaccination to adolescents. METHODS:Participants were a national sample of 1500 U.S. parents of adolescents ages 11-17 recruited in 2014-15. In an online survey, items informed by Diffusion of Innovatio...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2018.04.088
更新日期：2018-06-07 00:00:00
abstract：OBJECTIVES:With the re-emergence of chikungunya virus (CHIKV) in an explosive form and in the absence of a commercially available vaccine, we aimed to develop candidate vaccines employing recombinant E2 protein or chemically inactivated whole virus. DESIGN AND METHODS:E2 gene of CHIKV isolate of ECSA genotype was clon...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.07.072
更新日期：2012-09-21 00:00:00
abstract：:Surveillance of adverse events in the field represents an essential part of vaccination strategies. Safety studies conducted before vaccine licensure have often not enough power to detect rare or long-term adverse events. Furthermore, adverse events may be caused by incorrect procedures of storage or administration. C...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2004.01.013
更新日期：2004-05-07 00:00:00
abstract：BACKGROUND:The Global Vaccine Action Plan highlights the need for immunisation programmes to have sustainable access to predictable funding. A good understanding of current and future funding needs, commitments, and gaps is required to enhance planning, improve resource allocation and mobilisation, and to avoid funding...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.12.053
更新日期：2015-05-07 00:00:00
abstract：BACKGROUND:Vaccinations are an effective choice to stop disease outbreaks, including COVID-19. There is little research on individuals' COVID-19 vaccination decision-making. OBJECTIVE:We aimed to determine individual preferences for COVID-19 vaccinations in China, and to assess the factors influencing vaccination deci...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2020.12.009
更新日期：2021-01-08 00:00:00
abstract：:Flavobacterium psychrophilum is a well-known pathogen causing significant problems in aquaculture worldwide. In recent years an increasing number of disease outbreaks caused by F. psychrophilum has been reported on juvenile and post smolts of rainbow trout (Oncorhynchus mykiss) in Norway. The current study was perform...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2013.01.016
更新日期：2013-04-08 00:00:00
abstract：:In this work, we explored the potential of cationic solid lipid nanoparticles (cSLN) as efficient adjuvants for inactivated foot and mouth disease virus (iFMDV) vaccine. The cSLN were prepared by O/W emulsion method with Compritol 888 ATO as lipid matrix, and were modified by cationic lipid Didodecyldimethylammonium b...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2020.02.004
更新日期：2020-03-04 00:00:00
abstract：:Tuberculosis remains a major public health hazard worldwide, with neonates and young infants potentially more susceptible to infection than adults. BCG, the only vaccine currently available, provides some protection against tuberculous meningitis in children but variable efficacy in adults, and is not safe to use in i...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2016.10.065
更新日期：2016-12-07 00:00:00
abstract：:Uncertainty regarding the benefits of influenza vaccination may contribute to the underutilization of this vaccine. We have conducted serial cohort studies using the administrative data bases of a Twin Cities based managed care organization to assess the impact of disease and benefits of vaccination among the elderly....
journal_title：Vaccine
pub_type： 杂志文章,多中心研究
doi：10.1016/s0264-410x(99)00105-x
更新日期：1999-07-30 00:00:00
abstract：BACKGROUND:Human papillomavirus (HPV) vaccines have been recommended as primary prevention of HPV-related cancers for over 10 years in the United States, and evidence reveals decreased incidence of HPV infections following vaccination. However, concerns have been raised that HPV vaccines could decrease fertility. This ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2020.03.035
更新日期：2020-05-19 00:00:00
abstract：PURPOSE:We conducted a cancer vaccine clinical trial with MAGE-A4 protein. Safety, clinical response, and antigen-specific immune responses were analyzed and the prognostic factors by vaccination were investigated. EXPERIMENTAL DESIGN:Twenty patients with advanced esophageal, stomach or lung cancer were administered M...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.09.002
更新日期：2014-10-14 00:00:00
abstract：:Mucosal but not parenteral vaccination with whole Leishmania amazonensis promastigotes antigens (LaAg) is known to increase host resistance to infection by an as yet unknown immune mechanism. Since early immune responses are critical for infection establishment, in the present study the differential responses elicited...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2018.03.020
更新日期：2018-04-25 00:00:00
abstract：:A substantial rise in the number of AIDS cases has been observed during the past year in many African countries, and HIV-2 indigenous transmission and increased disease have since gained importance in Senegal. Both for HIV-1 and HIV-2 diagnosis, ELISA positivity is followed by a Western blot test for specific antibodi...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(88)90210-1
更新日期：1988-06-01 00:00:00
abstract：:We tested whether pilus proteins of rabbit diarrhoeagenic Escherichia coli (RDEC-1), incorporated into biodegradable microspheres, could function as safe and effective oral immunogens in the rabbit diarrhoea model. The RDEC-1 adhesin, AF/R1, incorporated into poly(D,L-lactide-co-glycolide) microspheres, was administer...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(93)90018-s
更新日期：1993-01-01 00:00:00
abstract：:Group W135 polysaccharide vaccines were licensed without efficacy trials using the serum bactericidal antibody (SBA) assay as a surrogate of protection. Standardization of group A and C SBA assays has been largely achieved. However, no such efforts have been focussed on W135. Although W135 strains have been recommende...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2007.04.043
更新日期：2007-09-03 00:00:00
abstract：:The oral, pentavalent rotavirus vaccine (PRV), RotaTeq was assessed for prevention of severe rotavirus gastroenteritis (RVGE) in young children in two multi-site, randomized, placebo-controlled field trials; one in Asia (Vietnam and Bangladesh) and the other in sub-Saharan Africa (Ghana, Kenya and Mali). The efficacy ...
journal_title：Vaccine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.vaccine.2011.11.094
更新日期：2012-04-27 00:00:00
abstract：:Plasmodium falciparum Pfs25 antigen, expressed on the surface of zygotes and ookinetes, is one of the leading targets for the development of a malaria transmission-blocking vaccine (TBV). Our laboratory has been evaluating DNA plasmid based Pfs25 vaccine in mice and non-human primates. Previously, we established that ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2013.05.006
更新日期：2013-06-28 00:00:00
abstract：:Since 1996, the scientific community has become aware of 14 reports of yellow fever vaccine (YEL)-associated viscerotropic disease (YEL-AVD) cases and four reports of YEL-associated neurotropic disease (YEL-AND) worldwide, changing our understanding of the risks of the vaccine. Based on 722 adverse event reports after...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.01.089
更新日期：2005-05-09 00:00:00
abstract：BACKGROUND:The demand on effective and safe anthrax vaccine is increasing as a part of the preparedness for possible bioterrorism in the future. We performed a randomized, single-blind, placebo controlled phase II clinical study to evaluate the immunogenicity and safety of a novel recombinant protective antigen (rPA) a...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2019.05.057
更新日期：2019-06-27 00:00:00
abstract：OBJECTIVES:To assess the effectiveness and cost-effectiveness of routine childhood vaccination by new vaccines against rotavirus in France. METHODS:We constructed a Markov decision tree to compare two alternatives: "no vaccination" and "vaccination". A hypothetical birth cohort of 750,000 children was followed until 3...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.11.064
更新日期：2008-01-30 00:00:00
abstract：:Since many years Afssaps applies the 3R's strategy (replacement, reduction and refinement) for the use of laboratory animal testing in the framework of vaccine batch release. In this context, for Hepatitis A vaccines, a study was carried out to assess the feasibility of replacing the in vivo "Gold Standard" potency as...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.12.006
更新日期：2010-02-17 00:00:00
abstract：BACKGROUND:We have developed a new oral vaccine against enterotoxigenic Escherichia coli (ETEC), which is the most common cause of bacterial diarrhea in children in developing countries and in travelers. METHODS:The vaccine was tested for safety and immunogenicity alone and together with double-mutant heat-labile toxi...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2014.10.069
更新日期：2014-12-12 00:00:00
abstract：AIM:To assess the impact of the introduction of rotavirus vaccination in New Zealand at a regional and national level, underlining the utility of a passively collected laboratory dataset. METHOD:Retrospective laboratory data for rotavirus testing from Wellington and Hutt Hospitals from 1 January 2010 to 31 December 20...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2017.07.018
更新日期：2017-08-16 00:00:00
abstract：BACKGROUND:Estimates of WHO and UNICEF vaccination coverage may provide little insight into the extent to which vaccinations are administered on time. Yet, lack of adherence to the recommended age to receive a specific vaccination may have detrimental health consequences. For example, delays in receiving vaccination wi...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.10.107
更新日期：2015-12-16 00:00:00
abstract：:A family of parasite antigens known as Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is believed to play an important role in the binding of infected erythrocytes to host receptors in the micro-vasculature. Available data advocates the existence of a subset of very adhesive (rosetting, auto-agglutinati...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.02.015
更新日期：2004-07-29 00:00:00
abstract：:The immunopotentiating activities of colloidal iron hydroxide, a novel, experimental mineral adjuvant, and of aluminium hydroxide. the licensed adjuvant for human vaccines, were compared. Our studies revealed that colloidal iron hydroxide and aluminium hydroxide behaved comparably with respect to supporting induction ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(98)00201-1
更新日期：1999-03-05 00:00:00
abstract：:The cattle tick, Rhipicephalus microplus, is regarded as the most economically important ectoparasite of livestock globally. Control is achieved primarily through the use of acaricides. This approach is hampered by the development of resistance to commercial acaricides among cattle tick populations. Vaccination agains...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.08.066
更新日期：2012-10-19 00:00:00
