Abstract:
:In this work, we explored the potential of cationic solid lipid nanoparticles (cSLN) as efficient adjuvants for inactivated foot and mouth disease virus (iFMDV) vaccine. The cSLN were prepared by O/W emulsion method with Compritol 888 ATO as lipid matrix, and were modified by cationic lipid Didodecyldimethylammonium bromide (DDAB). The content of cationic lipid was optimized to produce cSLN with appropriate particle size, surface morphology, zeta potential, and polydispersity. Loading iFMDV onto cSLN by electrostatic attraction did not destruct iFMDV particle structure as measured by high performance size exclusion chromatography (HPSEC). Differential scanning fluorimetry (DSF) showed the transition temperature, Tm, related to iFMDV dissociation increased for 1.2 °C after loading on cSLN, indicating an enhanced stability of this unstable antigen. The cSLN loaded iFMDV enhanced in vitro antigen uptake and activation of bone-marrow-derived dendritic cells (BMDCs) with augmented expression of CD86, CD40, and MHC I. In animal trials, BALB/c mice were immunized with free iFMDV, antigen adjuvanted with the cSLN, and antigen adjuvanted with Montanide ISA 206 emulsion. Specific antibody titers showed cSLN could stimulate similar FMDV-specific IgG and IgG subclasses antibody level compared with the widely used ISA 206. In addition, cSLN significantly enhanced memory immune response including effector-memory T cells and central-memory T cells compared to free iFMDV antigen and antigen adjuvanted with ISA 206. Taken together the enhanced humoral and T cell immune responses and the antigen structure friendly properties, cSLN can be a potential adjuvant for iFMDV vaccines.
journal_name
Vaccinejournal_title
Vaccineauthors
Li S,Yang Y,Lin X,Li Z,Ma G,Su Z,Zhang Sdoi
10.1016/j.vaccine.2020.02.004subject
Has Abstractpub_date
2020-03-04 00:00:00pages
2478-2486issue
11eissn
0264-410Xissn
1873-2518pii
S0264-410X(20)30157-2journal_volume
38pub_type
杂志文章相关文献
VACCINE文献大全abstract::Viral hepatitis remains a major contributor to the global disease burden. Mass immunisation strategies against hepatitis B have been adopted by more than 90 developing and industrialised countries. Countries with low hepatitis A endemicity are experiencing cyclical outbreaks and an epidemiological shift, with larger n...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(00)00193-6
更新日期:2000-10-15 00:00:00
abstract::Little is known of men's knowledge of cervical cancer and its links with human papillomavirus (HPV), or of their attitudes and beliefs about HPV vaccination. This is despite men's sexual behaviour contributing to HPV transmission and their potential role in deciding whether their children are vaccinated against HPV. T...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.02.101
更新日期:2009-05-14 00:00:00
abstract::In the last quarter of the year 2000, the meningococcal C conjugated vaccine was incorporated into the routine vaccination schedule in Catalonia (at 2, 4 and 6 months). In addition a vaccination campaign was carried out in children <6 years of age, with a coverage of 96.2%. The effectiveness of the vaccination in this...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00590-x
更新日期:2003-01-30 00:00:00
abstract::Influenza virus causes acute upper and lower respiratory infections and is the most likely, among known pathogens, to cause a large epidemic in humans. Influenza virus mutates rapidly, enabling it to evade natural and vaccine-induced immunity. Furthermore, influenza viruses can cross from animals to humans, generating...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.03.085
更新日期:2016-06-03 00:00:00
abstract::Carbopol is a polyanionic carbomer used in man for topical application and drug delivery purposes. However parenteral administration of Carbopol in animal models results in systemic adjuvant activity including strong pro-inflammatory type-1 T-cell (Th1) polarization. Here we investigated potential pathways of immune a...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.03.025
更新日期:2016-04-27 00:00:00
abstract::We examined potential risk factors on vaccine virus shedding and antibody seroresponse to human rotavirus vaccine (Rotarix) in Mexican infants. Two doses of Rotarix were administered to infants during the first two visits for their routine childhood immunization (∼8 and 15weeks of age) in Mexico City. Infant's charact...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.09.006
更新日期:2016-10-17 00:00:00
abstract::In the face of an almost unprecedented threat of a global pandemic of influenza it is imperative that stockpiling of appropriate drugs and devices begin now. One vital device is an appropriate syringe for delivering vaccine. With the potential for millions to be infected and the vaccine supply severely stretched it is...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.02.056
更新日期:2006-05-29 00:00:00
abstract::In Russia, live vaccine strains Brucella abortus 82 and B. abortus 75/79-AV have been widely and extensively utilized for specific prophylaxis of cattle brucellosis. To differentiate these vaccine strains from each other and laboratory collection of other vaccine (n=4), reference (n=15) and field Brucella strains (n=6...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.03.051
更新日期:2010-10-01 00:00:00
abstract:BACKGROUND:Measles is a highly infectious respiratory disease which causes 122,000 deaths annually. Although measles vaccine is extremely safe and effective, vaccine coverage could be improved by a vaccine that is more easily administered and transported. We developed an inhalable dry powder measles vaccine (MVDP) and ...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2014.09.071
更新日期:2014-11-28 00:00:00
abstract::Effective immunoprophylaxis directed against the pre-erythrocytic stages of the malaria parasite requires a vaccine that can elicit humoral and cell mediated immunity in individuals of diverse genetic background. In order for a synthetic peptide malaria vaccine to meet these requirements, problems associated with gene...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(97)00238-7
更新日期:1998-04-01 00:00:00
abstract::As a first step towards a vaccine against diarrhoeal disease caused by enterotoxigenic Escherichia coli (ETEC), we have studied the expression of several ETEC antigens in the live attenuated Vibrio cholerae vaccine strain CVD 103-HgR. Colonization factors (CF) CFA/I, CS3, and CS6 were expressed at the surface of V. ch...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.02.052
更新日期:2006-05-15 00:00:00
abstract::The potential outcomes of a Phase II schistosomiasis vaccine trial are explored using mathematical models which assume some natural immunity and a vaccine providing partial protection for a limited time. Analyses suggest that vaccination may have only a limited impact on life-long cumulative worm burdens and may lead ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(95)00083-d
更新日期:1995-08-01 00:00:00
abstract::We previously described that an inactivated vaccine against heartwater prepared from Cowdria ruminantium (Crystal Springs strain) organisms and administered in complete Freund's adjuvant (cFA) protected sheep against homologous needle challenge. Further studies, described herein, demonstrated that this vaccine protect...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(98)80120-5
更新日期:1998-07-01 00:00:00
abstract::Different subsets of dendritic cells (DC) elicit qualitatively different immune responses. In mice, two lymphoid tissue-resident subsets, CD8α(+) and CD8α(-), have been implicated in the induction of T helper 1 (Th1) or Th2 responses, respectively. Moreover, CD8α(+) DC appear to play a major role in priming CD8(+) T l...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.06.071
更新日期:2011-08-11 00:00:00
abstract::A mass vaccination campaign against influenza A/H1N1 was launched in France in October 2009. Vaccination was offered free of charge to the entire population according to a pre-defined order of priority. Demographic data and data on vaccinations given were recorded in a dedicated database. We analysed vaccine uptake by...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.09.096
更新日期:2010-11-29 00:00:00
abstract::Although studies have described factors associated with pertussis hospitalization in children, data on adult hospitalization are sparse. We examined the association between patient characteristics and hospitalization among older adults with pertussis. We conducted a nested case-control study of participants in the 45 ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.08.068
更新日期:2015-10-13 00:00:00
abstract::As part of a larger study exploring how European citizens' balance issues of public and private interest and the extent to which they are prepared to accept State intervention on a range of public health issues, focus group participants were asked whether childhood immunisation should be a matter of parental choice or...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.11.001
更新日期:2008-01-17 00:00:00
abstract::Therapeutic immunization of HIV-1-infected individuals with or without anti-retroviral therapy is a new promising disease prevention. To induce a new cytotoxic T(CD8) lymphocyte (CTL) immunity during chronic HIV-1 infection 15 infrequently targeted but conserved HLA-supertype binding CTL epitopes from Gag, Pol, Nef, E...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.07.025
更新日期:2011-09-16 00:00:00
abstract:BACKGROUND:The effectiveness of inactivated influenza vaccine (IIV) immunization in preventing all cause respiratory illness (RI) in children with pre-existing medical conditions has not been fully established and varies from season to season. This study aims to quantify the overall impact of IIV immunization on primar...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.11.086
更新日期:2020-04-09 00:00:00
abstract::Induction of broad HIV-1 neutralizing antibodies should be a major goal of an effective HIV-1 vaccine. However, B cells are severely damaged during HIV-1 infection with loss of memory B cells and decline of serological memory. The molecular events leading to B cell damage must be further characterized with the aim of ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2007.11.063
更新日期:2008-06-06 00:00:00
abstract::Mumps virus is still circulating and annual mumps outbreaks occur with fluctuating magnitudes in Japan. Aseptic meningitis has been reported after vaccination and it would be of importance to determine whether this was related to the vaccination. The objective of this study was to develop a sensitive, specific and rap...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.09.093
更新日期:2007-01-26 00:00:00
abstract::Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes acute encephalitis with high mortality in humans. To understand the virus-host interactions that influence JEV virulence, we determined the lethality of a neurovirulent (RP-9) and an attenuated (RP-2ms) variant of JEV in several immunodeficie...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.03.007
更新日期:2009-05-11 00:00:00
abstract:BACKGROUND:Evidence that respiratory infections trigger stroke suggests that influenza or pneumococcal vaccination might prevent stroke. We aimed to investigate whether influenza or pneumococcal vaccination or both together were associated with reduced risk of stroke or transient ischaemic attack (TIA). METHODS:We use...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.01.029
更新日期:2014-03-10 00:00:00
abstract::This work demonstrates the presence of immune regulatory cells in the cervical lymph nodes draining Bacillus Calmette-Guérin (BCG) vaccinated site on the dorsum of the ear in guinea pigs. It is shown that whole cervical lymph node cells did not proliferate in vitro in the presence of soluble mycobacterial antigens (PP...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.10.009
更新日期:2020-11-10 00:00:00
abstract::Development of meningococcal serogroups A, C, W-135 and Y conjugate vaccines could expand coverage against devastating meningococcal diseases. The immunogenicity of one dose of each one of five MenACWY-TT formulations versus a licensed ACWY polysaccharide vaccine was evaluated in 175 healthy subjects of 15-25 years. S...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2008.08.075
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Most developing countries are implementing the WHO immunisation programme. Although vaccines reach most children, many modifications of the recommended schedule are observed in practice. We investigated the association between vaccination status and risk of hospitalisation in Guinea-Bissau. METHODS:From May...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.03.016
更新日期:2011-05-09 00:00:00
abstract:BACKGROUND:Measles-containing vaccine (MCV) was introduced in 1965 in Beijing and given to the children aged 8m-14y. In population-based surveillance system, real-time polymerase chain reaction (RT-PCR) and immunoglobulin M(IgM) serology tests have been conducted for each suspected case since 2013. We used the surveill...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.05.058
更新日期:2019-06-27 00:00:00
abstract::Strict age limits for receipt of rotavirus vaccines and simultaneous use of vaccines requiring two (Rotarix(®)) and three (RotaTeq(®)) doses in Australia may impact on coverage and timeliness of other vaccines in the infant schedule. Using data from the Australian Childhood Immunisation Register (ACIR), coverage and t...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.02.007
更新日期:2013-04-08 00:00:00
abstract::Various peptide-based approaches to simultaneous induction of multiple cytotoxic T lymphocyte (CTL) responses were evaluated as part of ongoing efforts to develop immunotherapeutic vaccines for use in humans. To this end, HLA (human histocompatibility leukocyte antigen)-A2-restricted epitopes from several specific vir...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(97)00264-8
更新日期:1998-05-01 00:00:00
abstract::In 2003, a nationwide cross-sectional study was conducted to assess the current influenza vaccination coverage of the adult population of Germany, especially in persons belonging to defined target groups. Of 666 surveyed persons, 190 (adjusted 24%) had received influenza vaccination and 419 participants belonged to at...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.12.020
更新日期:2006-03-24 00:00:00