Testing for AIDS on samples of dried blood prepared on filter papers.


:A substantial rise in the number of AIDS cases has been observed during the past year in many African countries, and HIV-2 indigenous transmission and increased disease have since gained importance in Senegal. Both for HIV-1 and HIV-2 diagnosis, ELISA positivity is followed by a Western blot test for specific antibodies. The serum samples are stored at sub-zero temperatures both during shipment and storage while awaiting serological testing. Attempts have been made to use dried blood on filter papers for AIDS diagnosis, in 35 samples from Africa and 10 from USA, along with corresponding serum samples. The concordance has been good with an almost invariable ELISA confirmation on Western blot, no selective loss in any of the bands in Western blot and the Pearson coefficient value for ELISA testing on serum and dried blood lots of 0.85. Based on the data obtained during the intentional exposure of blood-impregnated filter papers, from nine HIV-positive and one HIV-negative patient from the USA, to varying temperatures and humidity for different intervals, blood collection on filter paper would appear to have emerged as a satisfactory procedure for HIV seroepidemiological studies.






Arya SC




Has Abstract


1988-06-01 00:00:00
















  • An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures.

    abstract::Yellow fever is an acute infectious disease caused by prototype virus of the genus Flavivirus. It is endemic in Africa and South America where it represents a serious public health problem causing epidemics of hemorrhagic fever with mortality rates ranging from 20% to 50%. There is no available antiviral therapy and v...


    pub_type: 杂志文章


    authors: Pereira RC,Silva AN,Souza MC,Silva MV,Neves PP,Silva AA,Matos DD,Herrera MA,Yamamura AM,Freire MS,Gaspar LP,Caride E

    更新日期:2015-08-20 00:00:00

  • Oral immunization of chickens with avirulent Salmonella vaccine strain carrying C. jejuni 72Dz/92 cjaA gene elicits specific humoral immune response associated with protection against challenge with wild-type Campylobacter.

    abstract::It is well documented that poultry and poultry products are the major source of human campylobacteriosis and salmonellosis. This study examined the general efficacy of avirulent Salmonella vaccine strains expressing Campylobacter antigen as a bivalent chicken vaccine prototype. Three C. jejuni genes: cjaA (cj0982c), c...


    pub_type: 杂志文章


    authors: Wyszyńska A,Raczko A,Lis M,Jagusztyn-Krynicka EK

    更新日期:2004-03-29 00:00:00

  • Structural perturbation of diphtheria toxoid upon adsorption to aluminium hydroxide adjuvant.

    abstract::Aluminium-containing adjuvants are often used to enhance the potency of vaccines. In the present work we studied whether adsorption of diphtheria toxoid to colloidal aluminium hydroxide induces conformational changes of the antigen. Diphtheria toxoid has a high affinity for the aluminium hydroxide particles based on a...


    pub_type: 杂志文章


    authors: Régnier M,Metz B,Tilstra W,Hendriksen C,Jiskoot W,Norde W,Kersten G

    更新日期:2012-11-06 00:00:00

  • Antibody-dependent phagocytosis (ADP) responses following trivalent inactivated influenza vaccination of younger and older adults.

    abstract::Globally the most commonly utilised immunisation against influenza is the trivalent inactivated influenza vaccine (TIV) derived from an A/H1N1, an A/H3N2 and aB type influenza virus. Vaccine effectiveness of TIV varies year to year, depending on how well antigenically matched the strains in the vaccine are compared to...


    pub_type: 杂志文章


    authors: Ana-Sosa-Batiz F,Johnston APR,Hogarth PM,Wines BD,Barr I,Wheatley AK,Kent SJ

    更新日期:2017-11-07 00:00:00

  • Phase II randomized, placebo-controlled trial of M. vaccae-derived protein (PVAC) for the treatment of psoriasis.

    abstract::The treatment effect against psoriasis of an antigen (delipidated, deglycolipidated form of M. vaccae-PVAC) was investigated. One hundred and sixty-five patients were enrolled in three arms (50 or 15 microg or placebo), each receiving a total of two intradermal injections (days 0 and 21). At week 12, a 75% decrease in...


    pub_type: 杂志文章,随机对照试验


    authors: Netto EM,Takahashi D,de Fátima Paim de Oliveira M,Barbosa P,Ferraz N,Paixão A,Oyafuso LK,Bortoletto C,Matos D,Paixão M,da Silva AO,Badaro R

    更新日期:2006-06-05 00:00:00

  • Maternal immunization with inactivated influenza vaccine: rationale and experience.

    abstract::Inactivated influenza vaccine is recommended for routine use in high-risk individuals in the United States, including women who will be in the second or third trimesters of pregnancy. The basis for this recommendation is the high risk of exposure and disease due to influenza viruses in pregnant women, as well as the i...


    pub_type: 杂志文章,评审


    authors: Englund JA

    更新日期:2003-07-28 00:00:00

  • Using QPCR to assign infectious potencies to adenovirus based vaccines and vectors for gene therapy: toward a universal method for the facile quantitation of virus and vector potency.

    abstract::The assignment of infectious potency to test articles of adenovirus has been conducted mainly using classical end-point dilution methods, which rely on virus induced cytopathology to reveal the presence of infectious virus. These assays suffer the disadvantages of labor intensity, duration, throughput restriction and ...


    pub_type: 杂志文章


    authors: Wang F,Puddy AC,Mathis BC,Montalvo AG,Louis AA,McMackin JL,Xu J,Zhang Y,Tan CY,Schofield TL,Wolf JJ,Lewis JA

    更新日期:2005-08-22 00:00:00

  • Colostral transmission of BTV-8 antibodies from dairy cows six years after vaccination.

    abstract::Bluetongue virus (BTV) antibodies were analysed in 27 Swiss calves born in 2016 at the age of 16-19 days using competitive enzyme-linked-immunosorbent-assay (cELISA) and virus neutralization test (VNT) (animal trial permission number: 75684). Obligatory documentation proved that 15 of 27 dams were BTV-8 vaccinated onc...


    pub_type: 杂志文章


    authors: Ayrle H,Mevissen M,Kaske M,Vögtlin A,Fricker R,Hoffmann B,Büttner M,Marinovic Z,Walkenhorst M

    更新日期:2018-09-18 00:00:00

  • The effect of early measles vaccination on thymic size. A randomized study from Guinea-Bissau.

    abstract:UNLABELLED:In low-income countries early measles vaccine (MV) is associated with reduced child mortality which cannot be explained by prevention of measles. A large thymus gland in infancy is also associated with reduced mortality. We hypothesized that early MV is associated with increased thymic size. Within a randomi...


    pub_type: 杂志文章,随机对照试验


    authors: Christensen LD,Eriksen HB,Biering-Sørensen S,Balé C,Do VA,Andersen A,Martins CL,Sodemann M,Aaby P,Benn CS

    更新日期:2014-03-26 00:00:00

  • AIDS and HIV vaccines.

    abstract::In spite of extensive prevention programs, the HIV epidemics is still spreading worldwide, in particularly in developing countries where clade C viruses predominate. WHO estimates that there are 16, 000 new cases of HIV infection daily and that 100 M individuals will be infected by the end of the next decade. In spite...


    pub_type: 杂志文章,评审


    authors: Klein M

    更新日期:1999-10-01 00:00:00

  • Inflammatory parameters associated with systemic reactogenicity following vaccination with adjuvanted hepatitis B vaccines in humans.

    abstract:BACKGROUND:Adjuvants like AS01B increase the immunogenicity of vaccines and generally cause increased transient reactogenicity compared with Alum. A phase II randomized trial was conducted to characterize the response to AS01B and Alum adjuvanted vaccines. A post-hoc analysis was performed to examine the associations b...


    pub_type: 杂志文章,随机对照试验


    authors: Burny W,Marchant A,Hervé C,Callegaro A,Caubet M,Fissette L,Gheyle L,Legrand C,Ndour C,Tavares Da Silva F,van der Most R,Willems F,Didierlaurent AM,Yarzabal J,ECR-008 study group.

    更新日期:2019-03-28 00:00:00

  • E-health use, vaccination knowledge and perception of own risk: drivers of vaccination uptake in medical students.

    abstract:OBJECTIVE:was to improve understanding of mechanisms contributing to healthcare personnel's (HCP) reluctance to get vaccinated against seasonal influenza. We assessed the role of several drivers: vaccination knowledge, vaccination recommendations and the role of the Internet (so-called e-health) in creating vaccination...


    pub_type: 杂志文章


    authors: Betsch C,Wicker S

    更新日期:2012-02-01 00:00:00

  • Modelling the epidemiological impact of rotavirus vaccination in Germany--a Bayesian approach.

    abstract:BACKGROUND:Rotavirus (RV) infection is the primary cause of severe gastroenteritis in children aged <5 years in Germany and worldwide. In 2013 the German Standing Committee on Vaccination (STIKO) developed a national recommendation for routine RV-immunization of infants. To support informed decision-making we predicted...


    pub_type: 杂志文章


    authors: Weidemann F,Dehnert M,Koch J,Wichmann O,Höhle M

    更新日期:2014-09-08 00:00:00

  • Can the vaccine adverse event reporting system be used to increase vaccine acceptance and trust?

    abstract::Vaccine refusal has an impact on public health, and the human pappillomavirus (HPV) vaccine is particularly underutilized. Research suggests that it may be difficult to change vaccine-related attitudes, and there is currently no good evidence to recommend any particular intervention strategy. One reason for vaccine he...


    pub_type: 杂志文章


    authors: Scherer LD,Shaffer VA,Patel N,Zikmund-Fisher BJ

    更新日期:2016-05-05 00:00:00

  • A randomized, phase 1/2 trial of the safety, tolerability, and immunogenicity of bivalent rLP2086 meningococcal B vaccine in healthy infants.

    abstract:BACKGROUND:Neisseria meningitidis serogroup B (MnB) is a major cause of invasive meningococcal disease in infants. A conserved, surface-exposed lipoprotein, LP2086 (a factor H-binding protein [fHBP]), is a promising MnB vaccine target. A bivalent, recombinant vaccine targeting the fHBP (rLP2086) of MnB was developed. ...


    pub_type: 杂志文章,随机对照试验


    authors: Martinon-Torres F,Gimenez-Sanchez F,Bernaola-Iturbe E,Diez-Domingo J,Jiang Q,Perez JL

    更新日期:2014-09-08 00:00:00

  • Algammulin, a new vaccine adjuvant comprising gamma inulin particles containing alum: preparation and in vitro properties.

    abstract::Crystallization of inulin with alum forms a fine (1-2 micron) suspension of electron-dense ovoids; the alum is embedded in inulin particles, which are then converted to the immune stimulant polymorphic form, gamma inulin. This very stable hybrid preparation is termed Algammulin. Preferred conditions for its preparatio...


    pub_type: 杂志文章


    authors: Cooper PD,Steele EJ

    更新日期:1991-05-01 00:00:00

  • Immunogenicity and safety of the inactivated Japanese encephalitis vaccine IXIARO® in elderly subjects: Open-label, uncontrolled, multi-center, phase 4 study.

    abstract:BACKGROUND:IXIARO® is a Vero cell-derived, inactivated Japanese encephalitis (JE) vaccine licensed mainly in western countries for children and adults traveling to JE endemic areas. Limited immunogenicity and safety data in elderly travelers have been available. OBJECTIVES:To evaluate safety and immunogenicity of IXIA...


    pub_type: 杂志文章,多中心研究


    authors: Cramer JP,Dubischar K,Eder S,Burchard GD,Jelinek T,Jilma B,Kollaritsch H,Reisinger E,Westritschnig K

    更新日期:2016-08-31 00:00:00

  • Safety and immunogenicity of prepandemic H5N1 influenza vaccines: a systematic review of the literature.

    abstract::A systematic review was performed to assess the safety and immunogenicity of the prepandemic H5N1 influenza vaccines licensed so far. A bibliographic search according to the COSI protocol was carried out and 8 of 235 potentially relevant publications were selected. Quality assessment was defined with both CASP and Jad...


    pub_type: 杂志文章,评审


    authors: Prieto-Lara E,Llanos-Méndez A

    更新日期:2010-06-11 00:00:00

  • Human IgG subclass responses in relation to serum bactericidal and opsonic activities after immunization with three doses of the Norwegian serogroup B meningococcal outer membrane vesicle vaccine.

    abstract::Ten adult volunteers, with low prevaccination levels of serum IgG antibodies against meningococcal antigens (< 1 microg ml(-1)), received three doses of the Norwegian group B meningococcal outer membrane vesicle (OMV) vaccine intramuscularly at weeks 0, 6 and 46. Anti-OMV IgG subclass responses were measured and compa...


    pub_type: 杂志文章


    authors: Naess LM,Aarvak T,Aase A,Oftung F,Høiby EA,Sandin R,Michaelsen TE

    更新日期:1999-02-26 00:00:00

  • DNA versus protein immunisation for production of monoclonal antibodies against Choristoneura fumiferana ecdysone receptor (CfEcR).

    abstract::The full-length ecdysone receptor cDNA of Choristoneura fumiferana (CfEcR-B) was cloned into bacterial expression systems and the recombinant protein was expressed either with a His-tag (His-EcR-B) or glutathione-S-transferase (GST) fusion (GST-EcR-B). The His-EcR-B was expressed mostly as insoluble aggregates, while ...


    pub_type: 杂志文章


    authors: Yang DH,Makhmoudova A,Arif BM,Feng Q,Retnakaran A,Palli SR,Krell PJ

    更新日期:2006-04-12 00:00:00

  • Varicella in French adolescents and adults: individual risk assessment and cost-effectiveness of routine vaccination.

    abstract::Age-specific force of varicella infection, hospitalisation and death rates in non-immune persons were calculated using an age-based mathematical model and national data for France. A cost-effectiveness model was then applied to hypothetical cohorts of persons aged 15-45 years with a negative or uncertain history of va...


    pub_type: 杂志文章


    authors: Hanslik T,Boëlle PY,Schwarzinger M,Carrat F,Freedberg KA,Valleron AJ,Flahault A

    更新日期:2003-09-08 00:00:00

  • RTS,S malaria vaccine efficacy and immunogenicity during Plasmodium falciparum challenge is associated with HLA genotype.

    abstract::Although RTS,S remains the most advanced malaria vaccine, the factors influencing differences in vaccine immunogenicity or efficacy between individuals or populations are still poorly characterised. The analyses of genetic determinants of immunogenicity have previously been restricted by relatively small sample sizes ...


    pub_type: 杂志文章


    authors: Nielsen CM,Vekemans J,Lievens M,Kester KE,Regules JA,Ockenhouse CF

    更新日期:2018-03-14 00:00:00

  • Persistence of antibody response to pneumococcal capsular polysaccharides in vaccinated long term-care residents in Brazil.

    abstract::To evaluate the immunogenicity of 23-valent pneumococcal polysaccharide vaccine in 52 nursing homes residents aged > or = 60 years, IgG antibodies to serotypes 1, 5, 6B, and 8 were measured by ELISA and compared before, and 1 and 12 months following vaccination. A significant immunological response for all serotypes w...


    pub_type: 杂志文章


    authors: Brandão AP,de Oliveira TC,de Cunto Brandileone MC,Gonçalves JE,Yara TI,Simonsen V

    更新日期:2004-12-21 00:00:00

  • A large observational study to concurrently assess persistence of measles specific B-cell and T-cell immunity in individuals following two doses of MMR vaccine.

    abstract::The measurement of measles-specific neutralizing antibodies, directed against the surface measles virus hemagglutinin and fusion proteins, is considered the gold standard in measles serology. We assessed functional measles-specific neutralizing antibody levels in a racially diverse cohort of 763 young healthy adolesce...


    pub_type: 杂志文章


    authors: Haralambieva IH,Ovsyannikova IG,O'Byrne M,Pankratz VS,Jacobson RM,Poland GA

    更新日期:2011-06-15 00:00:00

  • Leprosy--in pursuit of a vaccine.

    abstract::Clinical leprosy is characterized by varying manifestations between tuberculoid and lepromatous leprosy. In the former state the patient is able to elicit a cell-mediated immune response whereas in the latter, there is usually a humoral response. An understanding of this immunological balance is crucial in the search ...


    pub_type: 历史文章,杂志文章,评审


    authors: Stewart-Tull DE

    更新日期:1984-12-01 00:00:00

  • Introducing cholera vaccination in Asia, Africa and Haiti: a meeting report.

    abstract::Orally-administered cholera vaccine (OCV) has been increasingly examined as an additional tool to intervene against endemic and epidemic cholera. In 2013, short- and long-term field experience with OCV under nine distinctive field settings was reported from India, Bangladesh, Vietnam, Guinea, Haiti, and Thailand. Lead...




    authors: Hall RH,Sack DA

    更新日期:2015-01-15 00:00:00

  • Interchangeability and tolerability of two inactivated hepatitis A vaccines in Chinese children.

    abstract::In China, no data are available to evaluate the interchangeability between Chinese domestic inactivated hepatitis A vaccines (Healive) and imported inactivated hepatitis A vaccines (Havrix). A double-blind, randomized controlled study was to compare interchangeability and safety of Healive and Havrix among Chinese chi...


    pub_type: 杂志文章,随机对照试验


    authors: Zhang ZL,Zhu XJ,Wang X,Liang M,Sun J,Liu Y,Gao ZG,Wu JY,Dong XJ,Liu RK,Chen JT,Zhang YQ,Wang W,Zhang LP,Yin W

    更新日期:2012-06-08 00:00:00

  • Prenatal Tdap immunization and risk of maternal and newborn adverse events.

    abstract::Many countries recommend combined tetanus toxoid, reduced diphtheria toxoid and acellular pertussis immunization (Tdap) during pregnancy to stimulate transplacental transmission of pertussis antibodies to newborns. The immune system can be altered during pregnancy, potentially resulting in differing immunization risks...


    pub_type: 杂志文章


    authors: Layton JB,Butler AM,Li D,Boggess KA,Weber DJ,McGrath LJ,Becker-Dreps S

    更新日期:2017-07-24 00:00:00

  • Haptoglobin polymorphism and the immune response after hepatitis B vaccination.

    abstract::One hundred healthy Caucasian medical students (age 22 +/- 1 years) were vaccinated with a recombinant hepatitis B vaccine and their haptoglobin types were determined. A relationship between haptoglobin type and immune response to the vaccine was observed. Subjects with a 2-2 haptoglobin phenotype produced significant...


    pub_type: 临床试验,杂志文章


    authors: Louagie H,Delanghe J,Desombere I,De Buyzere M,Hauser P,Leroux-Roels G

    更新日期:1993-09-01 00:00:00

  • A novel, broad spectrum therapeutic HPV vaccine targeting the E7 proteins of HPV16, 18, 31, 45 and 52 that elicits potent E7-specific CD8T cell immunity and regression of large, established, E7-expressing TC-1 tumors.

    abstract::Persistent infection by high risk genotypes of human papillomavirus (HPV) is the cause of cervical cancer, which remains one of the most common cancers among women worldwide. In addition, there is a growing appreciation that high risk HPVs are associated with a number of other cancers including anogenital cancers as w...


    pub_type: 杂志文章


    authors: Wick DA,Webb JR

    更新日期:2011-10-13 00:00:00