Abstract:
BACKGROUND:We have developed a new oral vaccine against enterotoxigenic Escherichia coli (ETEC), which is the most common cause of bacterial diarrhea in children in developing countries and in travelers. METHODS:The vaccine was tested for safety and immunogenicity alone and together with double-mutant heat-labile toxin (dmLT) adjuvant in a double-blind, placebo-controlled Phase I study in 129 Swedish adults. The vaccine consists of four inactivated recombinant E. coli strains overexpressing the major ETEC colonization factors (CFs) CFA/I, CS3, CS5, and CS6 mixed with an LT B-subunit related toxoid, LCTBA. Volunteers received two oral doses of vaccine alone, vaccine plus 10 μg or 25 μg dmLT or placebo. Secretory IgA antibody responses in fecal samples and IgA responses in secretions from circulating intestine-derived antibody secreting cells were assessed as primary measures of vaccine immunogenicity. RESULTS:The vaccine was safe and well tolerated; adverse events were few and generally mild with no significant differences between subjects receiving placebo or vaccine with or without adjuvant. As many as 74% of subjects receiving vaccine alone and 83% receiving vaccine plus 10 μg dmLT showed significant mucosal IgA responses to all five primary vaccine antigens and about 90% of all vaccinees responded to at least four of the antigens. Subjects receiving vaccine plus 10 μg dmLT responded with significantly increased intestine-derived anti-CS6 responses compared to subjects receiving vaccine alone. CONCLUSIONS:The vaccine was safe and broadly immunogenic. dmLT further enhanced mucosal immune responses to CF antigens present in low amounts in the vaccine. Based on these encouraging results, the vaccine will be tested for safety and immunogenicity in different age groups including infants in Bangladesh and for protective efficacy in travelers.
journal_name
Vaccinejournal_title
Vaccineauthors
Lundgren A,Bourgeois L,Carlin N,Clements J,Gustafsson B,Hartford M,Holmgren J,Petzold M,Walker R,Svennerholm AMdoi
10.1016/j.vaccine.2014.10.069subject
Has Abstractpub_date
2014-12-12 00:00:00pages
7077-84issue
52eissn
0264-410Xissn
1873-2518pii
S0264-410X(14)01459-5journal_volume
32pub_type
杂志文章,随机对照试验相关文献
VACCINE文献大全abstract::Human papillomavirus (HPV) vaccination is now offered to adolescent girls in the UK. Adolescents over 16 years old are likely to make their own decision about the vaccination. The purpose of this cross-sectional study was to assess acceptability of HPV vaccination among female adolescents (16 -- 19 years) and investig...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.02.057
更新日期:2009-04-21 00:00:00
abstract::The tetanus and diphtheria vaccination programme in Finland has been running for 50 years. After primary doses, tetanus boosters have been offered to men in military service and decennial boosters recommended for all through the adult life. For 30 years a diphtheria booster was only offered to men in the military serv...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2009.06.080
更新日期:2009-08-27 00:00:00
abstract::The Global Meningococcal Initiative (GMI) is composed of an international group of scientists, clinicians and public health officials with expertise in meningococcal immunology, epidemiology and prevention. The primary goal of the GMI is the promotion of the global prevention of invasive meningococcal disease through ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.02.058
更新日期:2011-04-18 00:00:00
abstract::During the 2000/2001 influenza season in Japan, children ranging in age from 6 months to 13 years with fever exceeding 37.5 degrees C were recruited. Vaccine efficacy was evaluated by comparing the rates of pre-seasonal vaccination between groups stratified by fever severity. Seven hundred and sixty one patients (33.1...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究
doi:10.1016/j.vaccine.2006.01.067
更新日期:2006-04-24 00:00:00
abstract:BACKGROUND:Respiratory syncytial virus (RSV) is the major viral cause of infant and childhood lower respiratory tract disease worldwide. Defining the optimal target product profile (TPP) is complicated due to a wide range of possible vaccine properties, modalities and an incomplete understanding of the mechanism of nat...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.10.073
更新日期:2017-01-05 00:00:00
abstract:BACKGROUND:Currently, one lifetime booster of a 23-valent pneumococcal polysaccharide vaccine (PPV23) is recommended for those at highest risk of invasive pneumococcal disease (IPD) 3-5 years after initial vaccination. Due to a lack of evidence on multiple revaccinations, recommendations on repeat revaccination do not ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2015.01.060
更新日期:2015-03-10 00:00:00
abstract::We exploited the powerful adjuvant properties of cholera holotoxin (CT) to create a mucosally administered subunit vaccine against respiratory syncytial virus (RSV). A genetically detoxified mutant CT with an E to H substitution at amino acid 29 of the CT-A1 subunit (CT-E29H) was compared to wild type CT for toxicity ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(00)00058-x
更新日期:2000-06-01 00:00:00
abstract::We have found out that transfection of the RTG-2 cell line with the viral haemorrhagic septicaemia virus (VHSV) glycoprotein G (G(VHSV))-coding plasmid induces an anti-VHSV state, similar to that induced by poly I:C. Taking the advantage of the constitutive expression of toll-like receptor 9 gene (tlr9) in RTG-2 cells...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.11.021
更新日期:2011-01-17 00:00:00
abstract::Recipients of renal transplants were vaccinated with 20 ng hepatitis B surface antigen aluminium-adsorbed vaccine in order to gauge their resistance to hepatitis B infection, to which these patients are at high risk. The patients were given three 40 micrograms doses of the vaccine over a period of six months and their...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0264-410x(84)90039-2
更新日期:1984-12-01 00:00:00
abstract::The development of a safe and effective vaccine against enterotoxigenic Escherichia coli (ETEC) would be useful for travellers and for young children in endemic areas. A feasibility study of an enteral ETEC vaccine prototype consisting of colonization factor antigen II (CFA/II), containing two component antigens CS1 a...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/s0264-410x(94)80038-2
更新日期:1994-11-01 00:00:00
abstract::In the present study we assessed the capacity of recombinant E. coli- or plasmid-expressed chicken interferons (IFN) and chicken IL-1beta, to exert immunostimulatory activities for humoral immune responses, in day-old and adult chickens. We observed that both recombinant E. coli-expressed chicken IFN-alpha/beta and IF...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(99)00537-x
更新日期:2000-04-14 00:00:00
abstract::One of the greatest challenges to HCV vaccine development is the induction of effective immune responses using recombinant proteins or vectors. In order to better understand which vaccine-induced antibodies contribute to neutralization of HCV the quality of polyclonal anti-E1E2 antibody responses in immunized mice and...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.10.045
更新日期:2011-12-09 00:00:00
abstract::The use of gene guns in ballistically delivering DNA vaccine coated gold micro-particles to skin can potentially damage targeted cells, therefore influencing transfection efficiencies. In this paper, we assess cell death in the viable epidermis by non-invasive near infrared two-photon microscopy following micro-partic...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.08.067
更新日期:2006-05-22 00:00:00
abstract::In this study, we tested the hypothesis that adoptive transfer of dendritic cells (DCs) transfected ex vivo with mRNA encoding hepatitis C virus (HCV) NS3/4A would initiate potent HCV-specific protective immune responses in vivo. Murine DCs were transfected with NS3/4A mRNA or eGFP mRNA using either electroporation or...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.11.046
更新日期:2007-02-26 00:00:00
abstract::110 children who were given the complete course of the inactivated hepatitis A vaccine (Havrix) were followed up 10 years later. Age-matched healthy children who were not inoculated served as controls. One month after two primary injections, all children were positive for serum antibody. After 10 years, 99.09% of inoc...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.04.096
更新日期:2010-07-05 00:00:00
abstract:BACKGROUND:This phase I trial evaluated the safety and immunogenicity of a candidate tuberculosis vaccination regimen, ChAdOx1 85A prime-MVA85A boost, previously demonstrated to be protective in animal studies, in healthy UK adults. METHODS:We enrolled 42 healthy, BCG-vaccinated adults into 4 groups: low dose Starter ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.10.102
更新日期:2020-01-22 00:00:00
abstract::This study was aimed to evaluate the efficacy and immunogenicity of combined hepatitis B vaccination with plasma-derived vaccine (PDV) and recombinant vaccine (RV). A total of 329 infants was recruited, including 224 high-risk infants born to hepatitis B e antigen-positive mothers and 105 low-risk infants born to hepa...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(95)00108-d
更新日期:1995-12-01 00:00:00
abstract::We reported previously on the development of a Bacillus anthracis vaccine strain expressing high levels of recombinant protective antigen (rPA) [Cohen et al., Infec Immun 2000;68(8):4549-58]. To further explore the potential of the B. anthracis platform, we generated several attenuated strains expressing lethal toxin ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.11.077
更新日期:2005-12-01 00:00:00
abstract::In line with WHO objectives, the Lao Government is committed to eliminate measles by 2012. Yet from 1992 to 2007, the annual incidence of measles remained high while the vaccination coverage showed a wide diversity across provinces. A descriptive study was performed to determine factors affecting compliance with vacci...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.07.077
更新日期:2010-09-24 00:00:00
abstract::The toxicity of Clostridium difficile large clostridial toxin B (TcdB) can be reduced by many orders of magnitude by a combination of targeted point mutations. However, a TcdB mutant with five point mutations (referred to herein as mTcdB) still has residual toxicity that can be detected in cell-based assays and in-viv...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.06.032
更新日期:2015-01-01 00:00:00
abstract::A recombinant Plasmodium falciparum circumsporozoite (CS) antigen (rPfCSA) was produced in insect cells using a baculovirus expression vector containing the entire CS gene. This near full-length CS antigen was adsorbed onto aluminium phosphate for use as a malaria vaccine. In a study of safety and immunogenicity, 20 v...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/0264-410x(92)90047-n
更新日期:1992-01-01 00:00:00
abstract::A major challenge for poultry vaccination is the design of vaccines that protect against multiple pathogens via a single protective dose delivered through mass vaccination methods. In this investigation, we examined herpesvirus of turkeys (HVT) as a vaccine vector for delivery of haemagglutinin (HA) antigen of highly ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.08.115
更新日期:2011-10-26 00:00:00
abstract::Recently avian influenza A viruses of the H5N1 subtype were shown to infect humans in the Hong Kong area, resulting in the death of six people. Although these viruses did not efficiently spread amongst humans, these events illustrated that influenza viruses of subtypes not previously detected in humans could be at the...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(98)00390-9
更新日期:1999-03-17 00:00:00
abstract::Rift Valley fever virus (RVFV), a member of the family Bunyaviridae, causes severe to fatal disease in newborn ruminants, as well as abortions in pregnant animals; both preventable by vaccination. Availability of a challenge model is a pre-requisite for vaccine efficacy trials. Several modes of inoculation with RVFV Z...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.02.066
更新日期:2014-04-25 00:00:00
abstract::For middle and low-income countries, the cost of HPV vaccines remains challenging. We conducted an open-label nonrandomized clinical trial evaluating immune response to the HPV-16/18 AS04-adjuvanted vaccine administered on a standard (months (M) 0-1-6) versus extended schedule (M 0-6-60) at 7, 21, 60, 72 and 120 month...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/j.vaccine.2013.11.059
更新日期:2014-02-03 00:00:00
abstract:INTRODUCTION:Despite the enormous benefits of childhood and maternal immunisation to individual and population health, the uptake of maternal vaccines during pregnancy remains suboptimal. Midwives are a trusted information source for parents and play an important role in the provision of immunisation information. Under...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.10.006
更新日期:2020-01-10 00:00:00
abstract:BACKGROUND:MenB-4C (Bexsero®) is a multicomponent serogroup B meningococcal vaccine. For vaccine licensure, efficacy was inferred from serum bactericidal antibody (SBA) against three antigen-specific indicator strains. The bactericidal role of antibody to the fourth vaccine antigen, Neisserial Heparin binding antigen (...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.06.020
更新日期:2017-07-24 00:00:00
abstract::Rotavirus vaccine was introduced in El Salvador in 2006 and is recommended to be given concomitantly with DTP-HepB-Haemophilus influenzae type b (pentavalent) vaccine at ages 2 months (upper age limit 15 weeks) and 4 months (upper age limit 8 months) of age. However, rotavirus vaccination coverage continues to lag beh...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.07.092
更新日期:2015-11-27 00:00:00
abstract:BACKGROUND:Vaccination coverage with tetanus toxoid, reduced diphtheria toxoid, and acellular pertussis (Tdap) vaccine in pregnancy or immediately postpartum has been low. Limited data exist on rigorously evaluated interventions to increase maternal vaccination, including Tdap. Tailored messaging based on the Elaborati...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2017.01.037
更新日期:2017-03-13 00:00:00
abstract::Praziquantel (PZQ), which is used to treat all forms of schistosomiasis, has been shown to induce strong T cell activities and decrease T regulatory cell levels. In our study, we investigated whether PZQ may be used as an adjuvant for a hepatitis B surface antigen (HBsAg) DNA vaccine (pcD-S2) in eliciting strong humor...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.10.101
更新日期:2010-02-23 00:00:00