当前位置： SCI文献检索 > VACCINE期刊下所有文献 > In vitro analysis of the factors contributing to the antiviral state induced by a plasmid encoding the viral haemorrhagic septicaemia virus glycoprotein G in transfected trout cells.

In vitro analysis of the factors contributing to the antiviral state induced by a plasmid encoding the viral haemorrhagic septicaemia virus glycoprotein G in transfected trout cells.

Abstract：

:We have found out that transfection of the RTG-2 cell line with the viral haemorrhagic septicaemia virus (VHSV) glycoprotein G (G(VHSV))-coding plasmid induces an anti-VHSV state, similar to that induced by poly I:C. Taking the advantage of the constitutive expression of toll-like receptor 9 gene (tlr9) in RTG-2 cells, we have investigated whether this antiviral state was induced by the cytosine-phosphodiester-guanine (CpG) motifs present in the plasmid DNA, by the endogenous expression of G(VHSV) protein or by both elements. For that, we have analysed the expression profile of the rainbow trout tlr9 and several genes related to TLR9-mediated immune response in the absence or presence of a lysosomotropic drug that specifically blocks TLR9-CpG DNA interaction. The results suggested that the high levels of cell protection conferred by a plasmid encoding G(VHSV) gene are due to G(VHSV) rather than to the CpG motifs within plasmid DNA. Therefore, plasmid DNA might not play a key role in the immune response elicited by DNA vaccines or perhaps other receptors instead TLR9 could be implicated in CpG motifs recognition and signalling. In addition, since RTG-2 cells express tlr9 gene, this cell line could be a good tool for screening TLR9 agonists, such as the immunomodulatory oligonucleotides (IMOs), as fish DNA vaccine adjuvants.

journal_name

Vaccine

journal_title

Vaccine

authors

Ortega-Villaizan M,Chico V,Martinez-Lopez A,Falco A,Perez L,Coll JM,Estepa A

doi

10.1016/j.vaccine.2010.11.021

subject

Has Abstract

pub_date

2011-01-17 00:00:00

pages

737-43

issue

4

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(10)01629-4

journal_volume

29

pub_type

杂志文章

相关文献

VACCINE文献大全
  • Adverse events following immunization in patients with primary immunodeficiencies.

    abstract：BACKGROUND:Adverse events following immunization (AEFI) requires special consideration in patients with primary immunodeficiency diseases (PID) because they may represent a "red flag" for the initial diagnosis and may cause disease complications. Therefore, the definition of appropriate vaccination schemes is a major i...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2016.01.047

    authors： Sarmiento JD,Villada F,Orrego JC,Franco JL,Trujillo-Vargas CM

    更新日期：2016-03-18 00:00:00

  • Safety of yellow fever vaccine administration in confirmed egg-allergic patients.

    abstract：:Yellow fever vaccine (YFV) is recommended in endemic areas but represents a risk for egg-allergic patients, as it is cultivated in embryonated eggs. This study aims to describe the outcomes of yellow fever vaccination in patients with confirmed egg allergy (EA). Methods:A prospective study was conducted from January 2...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2020.08.020

    authors： Gerhardt CMB,Castro APBM,Pastorino AC,Dorna MB,Nunes-Santos CJ,Aquilante BP,Miyaji KT,Lopes MH

    更新日期：2020-09-29 00:00:00

  • Willingness to participate in Ebola viral disease vaccine trials and receive vaccination by health workers in a tertiary hospital in Ile-Ife, Southwest Nigeria.

    abstract：BACKGROUND:Ebola viral disease (EVD) epidemic need to be contained through means which include vaccination of susceptible population. Vaccination has eradicated major killer diseases. OBJECTIVE:The study determined the health workers willingness to participate in EVD vaccine clinical trials and receive EVD vaccine. M...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2016.10.004

    authors： Olowookere SA,Abioye-Kuteyi EA,Adekanle O

    更新日期：2016-11-11 00:00:00

  • Vaccination status and sequence of vaccinations as risk factors for hospitalisation among outpatients in a high mortality country.

    abstract：BACKGROUND:Most developing countries are implementing the WHO immunisation programme. Although vaccines reach most children, many modifications of the recommended schedule are observed in practice. We investigated the association between vaccination status and risk of hospitalisation in Guinea-Bissau. METHODS:From May...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.03.016

    authors： Biai S,Rodrigues A,Nielsen J,Sodemann M,Aaby P

    更新日期：2011-05-09 00:00:00

  • Mumps disease in Beijing in the era of two-dose vaccination policy, 2005-2016.

    abstract：BACKGROUND:Since the introduction of mumps-containing vaccines (MuCV) in 1995 in Beijing, two-dose MuCV vaccination policy has been used, with the 1st and 2nd doses given at 18 months and 6 years of age, respectively. METHODS:Mumps epidemiology during 2005-2016 was described using surveillance data. Vaccine effectiven...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.03.074

    authors： Ma R,Lu L,Zhou T,Pan J,Chen M,Pang X

    更新日期：2018-05-03 00:00:00

  • Vaccination of sheep against M. paratuberculosis: immune parameters and protective efficacy.

    abstract：:Johne's disease in ruminants is caused by the pathogenic bacterium Mycobacterium avium subspecies paratuberculosis (Map). Currently available Map commercial vaccines protect against clinical disease but not infection. In this study, the proprietary Johne's vaccine Neoparasec and an aqueous formulation of Map 316F (Aqu...

    journal_title：Vaccine

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.vaccine.2005.05.031

    authors： Begg DJ,Griffin JF

    更新日期：2005-10-10 00:00:00

  • Characterization of a novel live attenuated infectious bronchitis virus vaccine candidate derived from a Korean nephropathogenic strain.

    abstract：:A nephropathogenic K2/01 strain of infectious bronchitis virus (IBV) was attenuated by 170 serial passages in embryonated chicken eggs for possible use as a future IBV vaccine strain. High-growth properties and narrow tissue tropisms (limited replication in respiratory tracts) were achieved by the adaptation process. ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2010.01.062

    authors： Lee HJ,Youn HN,Kwon JS,Lee YJ,Kim JH,Lee JB,Park SY,Choi IS,Song CS

    更新日期：2010-04-01 00:00:00

  • Improvement in attitudes toward influenza vaccination in medical students following an integrated curricular intervention.

    abstract：INTRODUCTION:Vaccination of health care workers (HCW) reduces transmission of influenza among patients, yet uptake of vaccination remains low. If vaccination education is integrated into the early medical school curriculum, will student attitudes toward the vaccine change? The objectives of the study were to: (1) Deter...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2013.11.043

    authors： Afonso N,Kavanagh M,Swanberg S

    更新日期：2014-01-16 00:00:00

  • Broad vaccine protection against Neisseria meningitidis using factor H binding protein.

    abstract：:Neisseria meningitidis, the causative agent of invasive meningococcal disease (IMD), is classified into different serogroups defined by their polysaccharide capsules. Meningococcal serogroups A, B, C, W, and Y are responsible for most IMD cases, with serogroup B (MenB) causing a substantial percentage of IMD cases in ...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2020.08.031

    authors： Findlow J,Bayliss CD,Beernink PT,Borrow R,Liberator P,Balmer P

    更新日期：2020-11-17 00:00:00

  • A randomized double-blind trial comparing a two-component acellular to a whole-cell pertussis vaccine in Senegal.

    abstract：:A randomized, double-blind trial comparing a diphtheria-tetanus-acellular pertussis vaccine (DTaP) (pertussis toxoid and filamentous hemagglutinin) with a whole-cell vaccine (DTwP) was conducted. A case-contact study was nested in the trial to estimate absolute efficacy. From 1990 through 1994, 4181 children were rand...

    journal_title：Vaccine

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0264-410x(97)00100-x

    authors： Simondon F,Preziosi MP,Yam A,Kane CT,Chabirand L,Iteman I,Sanden G,Mboup S,Hoffenbach A,Knudsen K,Guiso N,Wassilak S,Cadoz M

    更新日期：1997-10-01 00:00:00

  • Midwives' perceptions of vaccines and their role as vaccinators: The emergence of a new immunization corps.

    abstract：BACKGROUND:In France, midwives have recently been authorized to administer various vaccines to women (including pregnant women), newborns, and their family members. This is expected to enhance vaccine coverage. However, the French high level of vaccine hesitancy is also observed in some healthcare workers. We thus aime...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.06.050

    authors： Massot E,Epaulard O

    更新日期：2018-08-16 00:00:00

  • Safety of varying dosages of 7-valent pneumococcal protein conjugate vaccine in seniors previously vaccinated with 23-valent pneumococcal polysaccharide vaccine.

    abstract：:In a phase I/II dose escalation study, varying volumes (0.1 ml, 0.5 ml, 1.0 ml and 2.0 ml) of 7-valent pneumococcal conjugate vaccine (PCV) (Prevnar or 0.5 ml of 23-valent pneumococcal polysaccharide vaccine (PPV) were administered to 220 adults 70 through 79 years of age previously vaccinated with 0.5 ml PPV at age 6...

    journal_title：Vaccine

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.vaccine.2005.02.017

    authors： Jackson LA,Neuzil KM,Whitney CG,Starkovich P,Dunstan M,Yu O,Nelson JC,Feikin DR,Shay DK,Baggs J,Carste B,Nahm MH,Carlone G

    更新日期：2005-05-25 00:00:00

  • Immunogenicity of candidate hepatitis E virus DNA vaccine expressing complete and truncated ORF2 in mice.

    abstract：:Hepatitis E virus (HEV) is a major cause of enterically transmitted acute hepatitis of adults in developing nations. Our present studies show that, the complete ORF2 gene (1-660 amino acids, a.a.) coding for capsid protein of HEV as candidate DNA vaccine induced significant specific humoral and cellular immune respons...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.03.040

    authors： Deshmukh TM,Lole KS,Tripathy AS,Arankalle VA

    更新日期：2007-05-30 00:00:00

  • Implementation of a Q fever vaccination program for high-risk patients in the Netherlands.

    abstract：BACKGROUND:Between 2007 and 2011 the Netherlands was faced with an unprecedented Q fever outbreak with more than 4000 people affected. Dairy goats were considered the main source of infection. In addition to taking veterinary measures, the Dutch government offered an unlicensed vaccine against the causative bacterium C...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2013.03.062

    authors： Isken LD,Kraaij-Dirkzwager M,Vermeer-de Bondt PE,Rümke HC,Wijkmans C,Opstelten W,Timen A

    更新日期：2013-05-28 00:00:00

  • Effects of Taishan Pinus massoniana pollen polysaccharide on immune response of rabbit haemorrhagic disease tissue inactivated vaccine and on production performance of Rex rabbits.

    abstract：:Varied doses of Taishan Pinus massoniana pollen polysaccharide (TPPPS) and Astragalus polysaccharide (APS) extracted by hot water extraction and ethanol precipitation method were added to the vaccine in order to prepare polysaccharide-rabbit haemorrhagic disease (RHD) tissue inactivated vaccine. The purpose was to stu...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.01.068

    authors： Wei K,Sun Z,Yan Z,Tan Y,Wang H,Zhu X,Wang X,Sheng P,Zhu R

    更新日期：2011-03-21 00:00:00

  • Development and preclinical safety evaluation of a new therapeutic HIV-1 vaccine based on 18 T-cell minimal epitope peptides applying a novel cationic adjuvant CAF01.

    abstract：:Therapeutic immunization of HIV-1-infected individuals with or without anti-retroviral therapy is a new promising disease prevention. To induce a new cytotoxic T(CD8) lymphocyte (CTL) immunity during chronic HIV-1 infection 15 infrequently targeted but conserved HLA-supertype binding CTL epitopes from Gag, Pol, Nef, E...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.07.025

    authors： Fomsgaard A,Karlsson I,Gram G,Schou C,Tang S,Bang P,Kromann I,Andersen P,Andreasen LV

    更新日期：2011-09-16 00:00:00

  • Estimates of the effectiveness of a whole-cell pertussis vaccine from an outbreak in an immunized population.

    abstract：:Pertussis has re-emerged as a public health problem in Canada in recent years, emphasizing concerns about the effectiveness of the currently licensed whole-cell vaccine. Following a 1994 outbreak in Nova Scotia, we conducted a case-control study of 483 children aged < 10 years to assess vaccine effectiveness. Ninety-t...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(96)00176-4

    authors： Bentsi-Enchill AD,Halperin SA,Scott J,MacIsaac K,Duclos P

    更新日期：1997-02-01 00:00:00

  • Immunity to rubella before and after vaccination against measles, mumps and rubella (MMR) at 12 years of age of the first generation offered MMR vaccination in Sweden at 18 months.

    abstract：:In 1982, a two-dose programme of vaccination against measles, mumps and rubella (MMR) at the ages of 18 months and 12 years was introduced in Sweden. In 1992-3, the first group of children vaccinated at 18 months reached the age of 12, i.e. the time for a second dose. In connection with this 12-year vaccination, 376 c...

    journal_title：Vaccine

    pub_type： 杂志文章,多中心研究

    doi：10.1016/0264-410x(95)00143-o

    authors： Böttiger M

    更新日期：1995-12-01 00:00:00

  • Levofloxacin rescues mice from lethal intra-nasal infections with virulent Francisella tularensis and induces immunity and production of protective antibody.

    abstract：:The ability to protect mice against respiratory infections with virulent Francisella tularensis has been problematic and the role of antibody-versus-cell-mediated immunity controversial. In this study, we tested the hypothesis that protective immunity can develop in mice that were given antibiotic therapy following in...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.09.077

    authors： Klimpel GR,Eaves-Pyles T,Moen ST,Taormina J,Peterson JW,Chopra AK,Niesel DW,Carness P,Haithcoat JL,Kirtley M,Nasr AB

    更新日期：2008-12-09 00:00:00

  • Is there a causal link between hepatitis B vaccination and multiple sclerosis?

    abstract：:After the publication of case reports of hepatitis B vaccinees with onset or relapse of multiple sclerosis (MS), followed by a media-driven scare campaign in France, the perception that hepatitis B vaccine causes MS has developed. This has led to a fall in the acceptance of hepatitis B vaccination particularly in Fren...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(99)00533-2

    authors： Monteyne P,André FE

    更新日期：2000-04-03 00:00:00

  • Precise answers to the wrong question: prospective clinical trials and the meta-analyses of pneumococcal vaccine in elderly and high-risk adults.

    abstract：:Ten prospective clinical trials conducted in elderly and high-risk adults have failed to show that pneumococcal vaccine prevents pneumococcal bacteraemia and all pneumonia. Several of these trials focused on unrepresentative populations and most had serious methodological problems. Few adequately considered sample siz...

    journal_title：Vaccine

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.vaccine.2003.09.027

    authors： Fedson DS,Liss C

    更新日期：2004-02-25 00:00:00

  • Cost-effectiveness of childhood rotavirus vaccination in Taiwan.

    abstract：OBJECTIVE:Rotavirus is the most common cause of severe diarrhea in children. Two rotavirus vaccines (RotaTeq and Rotarix) have been licensed in Taiwan. We have investigated whether routine infant immunization with either vaccine could be cost-effective in Taiwan. METHODS:We modeled specific disease outcomes including ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.01.023

    authors： Wu CL,Yang YC,Huang LM,Chen KT

    更新日期：2009-03-04 00:00:00

  • Penton base induces better protective immune responses than fiber and hexon as a subunit vaccine candidate against adenoviruses.

    abstract：:Human adenoviruses (AdVs) have been extensively studied as vectors for gene therapy and vaccination. However, little attention has been paid to AdV vaccine development and treatment. Currently, there is a lack of information concerning the immunogenicity of AdV major capsid proteins. Here, using AdV7 as a model, we co...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.05.118

    authors： Hu K,Fu M,Guan X,Zhang D,Deng X,Xiao Y,Chen R,Liu H,Hu Q

    更新日期：2018-07-05 00:00:00

  • Evaluation of endotoxin content of diphtheria-tetanus-acellular pertussis combined (DTaP) vaccines that interfere with the bacterial endotoxin test.

    abstract：:Applicability of the endotoxin test to diphtheria-tetanus-acellular pertussis combined (DTaP) vaccines was examined. We found some DTaP vaccines that strongly interfered with Limulus amoebocyte lysate (LAL) activity of endotoxin without affecting lethal activity of endotoxin in D-galactosamine-treated mice. LAL activi...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(03)00006-9

    authors： Ochiai M,Kataoka M,Toyoizumi H,Kamachi K,Yamamoto A,Horiuchi Y

    更新日期：2003-05-16 00:00:00

  • Evaluation of 12 stabilizers in a developed attenuated Salmonella Enteritidis vaccine.

    abstract：:The development of a stable live attenuated Salmonella Enteritidis (SE) vaccine, resisting heat stress during transportation and storage in unequipped tropical and subtropical zones of the world, is highly recommended. Twelve stabilizers were individually supplemented into a 9 ml volume of sterile distilled water resu...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(02)00107-x

    authors： Barbour EK,Abdelnour A,Jirjis F,Faroon O,Farran MT

    更新日期：2002-05-22 00:00:00

  • Improving iSC performance through outsourcing - Considerations for using third-party service providers to increase innovation, capacity and efficiency.

    abstract：:Development partners and donors have encouraged and incentivized governments in developing countries to explore ways of working with third-party service suppliers to reduce costs and increase service delivery capacity. The distribution of vaccines and medicines has for a long time shown demand for outsourcing but publ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2016.11.108

    authors： Wright M,Forster G,Beale J

    更新日期：2017-04-19 00:00:00

  • Inclusion of the murine IgGκ signal peptide increases the cellular immunogenicity of a simian adenoviral vectored Plasmodium vivax multistage vaccine.

    abstract：INTRODUCTION:Cellular and humoral immune responses are both involved in protection against Plasmodium infections. The only malaria vaccine available, RTS,S, primarily induces short-lived antibodies and targets only a pre-erythrocytic stage antigen. Inclusion of erythrocytic stage targets and enhancing cellular immunoge...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.03.091

    authors： Fonseca JA,McCaffery JN,Caceres J,Kashentseva E,Singh B,Dmitriev IP,Curiel DT,Moreno A

    更新日期：2018-05-11 00:00:00

  • Immunization with the RTS,S/AS malaria vaccine induces IFN-γ(+)CD4 T cells that recognize only discrete regions of the circumsporozoite protein and these specificities are maintained following booster immunizations and challenge.

    abstract：:In a Phase 2a trial of the RTS,S/AS vaccine, we described significant association between protection against infection and vaccine-induced CD4 T cells. To determine whether processing of the circumsporozoite protein as a component of the RTS,S particulate antigen yields the same HLA-DR-restricted epitopes as those rec...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.09.098

    authors： Schwenk R,Lumsden JM,Rein LE,Juompan L,Kester KE,Heppner DG,Krzych U

    更新日期：2011-11-08 00:00:00

  • Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates.

    abstract：:The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than f...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(00)00439-4

    authors： Ohara N,Matsuoka M,Nomaguchi H,Naito M,Yamada T

    更新日期：2001-02-28 00:00:00

  • Chitosan alters inactivated respiratory syncytial virus vaccine elicited immune responses without affecting lung histopathology in mice.

    abstract：:Chitosan is a polysaccharide capable of augmenting immune responses with a proven safety record in animals and humans. These properties make it a potentially attractive agent for the prevention and treatment of infectious disease. Infection by respiratory syncytial virus (RSV) is the leading cause of serious lower res...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2019.06.003

    authors： Muralidharan A,Russell MS,Larocque L,Gravel C,Sauvé S,Chen Z,Li C,Chen W,Cyr T,Rosu-Myles M,Wang L,Li X

    更新日期：2019-07-09 00:00:00