Characterization of a novel live attenuated infectious bronchitis virus vaccine candidate derived from a Korean nephropathogenic strain.


:A nephropathogenic K2/01 strain of infectious bronchitis virus (IBV) was attenuated by 170 serial passages in embryonated chicken eggs for possible use as a future IBV vaccine strain. High-growth properties and narrow tissue tropisms (limited replication in respiratory tracts) were achieved by the adaptation process. Unlike the parent strain, the attenuated strain (K2p170) was safe in day-old specific-pathogen-free chicks since replication of the virus did not induce mortality and nephritis, and rarely induced histological changes in the trachea and kidney after intraocular administration. In day-old broilers, even though coarse spray administration of K2p170 induced clinical signs, ciliostasis, and histopathological lesions in the trachea and the kidney, they were all comparable to birds vaccinated with commercial H120 vaccine. Despite restriction of viral replication in the respiratory tract, K2p170 elicited the production of antiserum with a neutralization index of 4.5. K2p170 provided almost complete protection against both two distinct subgroups of Korean nephropathogenic strain (KM91-like and QX-like subgroup). Furthermore, K2p170 provided significantly greater cross-protection against two heterologous strains (Massachusetts and Korean respiratory strain) than those conferred by the commercial H120 vaccine. K2p170 also had no virulence reversion after five back passages in chickens. In conclusion, K2p170 exhibits a fine balance between attenuation and immunogenicity, possesses cross-protective efficacy, and merits further investigation as a potential live vaccine as an alternative means of protection against the recently emergent nephropathogenic IBV infection in many Eurasian countries.






Lee HJ,Youn HN,Kwon JS,Lee YJ,Kim JH,Lee JB,Park SY,Choi IS,Song CS




Has Abstract


2010-04-01 00:00:00
















  • United States of America Department of Health and Human Services support for advancing influenza vaccine manufacturing in the developing world.

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    pub_type: 杂志文章,评审


    authors: Perdue ML,Bright RA

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    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


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  • Effectiveness of human papillomavirus vaccine against incident and persistent infections among young girls: Results from a longitudinal Dutch cohort study.

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    pub_type: 杂志文章


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    authors: Liu Q,Zhang C,Zheng X,Shao X,Zhang X,Zhang Q,Jiang X

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Bungener L,Geeraedts F,Ter Veer W,Medema J,Wilschut J,Huckriede A

    更新日期:2008-05-02 00:00:00

  • Vaccination with recombinant Modified Vaccinia Ankara (MVA) viruses expressing single African horse sickness virus VP2 antigens induced cross-reactive virus neutralising antibodies (VNAb) in horses when administered in combination.

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    authors: Manning NM,Bachanek-Bankowska K,Mertens PPC,Castillo-Olivares J

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  • Polio Eradication Initiative: Contribution to improved communicable diseases surveillance in WHO African region.

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    authors: Mwengee W,Okeibunor J,Poy A,Shaba K,Mbulu Kinuani L,Minkoulou E,Yahaya A,Gaturuku P,Landoh DE,Nsubuga P,Salla M,Mihigo R,Mkanda P

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  • Improving adolescent HPV vaccination in a randomized controlled cluster trial using the 4 Pillars™ practice Transformation Program.

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    pub_type: 杂志文章,随机对照试验


    authors: Zimmerman RK,Moehling KK,Lin CJ,Zhang S,Raviotta JM,Reis EC,Humiston SG,Nowalk MP

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    pub_type: 杂志文章


    authors: Sambala EZ,Wiyeh AB,Ngcobo N,Machingaidze S,Wiysonge CS

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  • Outer membrane vesicles derived from Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection.

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    authors: Bottero D,Gaillard ME,Errea A,Moreno G,Zurita E,Pianciola L,Rumbo M,Hozbor D

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    abstract::Streptococcus pneumoniae (pneumococcus) is responsible for significant morbidity and mortality in worldwide. After introduction of current pneumococcal vaccines, a marked decrease in the incidence of pneumococcal disease was observed. Unfortunately, serotype shifts in carriage and disease, including capsular switch an...


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  • A single dose glycoprotein D-based subunit vaccine against pseudorabies virus infection.

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    authors: Zhang T,Liu Y,Chen Y,Wang A,Feng H,Wei Q,Zhou E,Zhang G

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  • An E2 sub-unit marker vaccine does not prevent horizontal or vertical transmission of classical swine fever virus.

    abstract::An experimental infection with classical swine fever (CSF) virus in E2 sub-unit marker vaccine vaccinated gilts was conducted in order to evaluate the effect of vaccination on virus transmission and course of the disease. Therefore, clinical signs as well as horizontal and vertical virus transmission were monitored in...


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    authors: Dewulf J,Laevens H,Koenen F,Mintiens K,de Kruif A

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    authors: El Bissati K,Zhou Y,Dasgupta D,Cobb D,Dubey JP,Burkhard P,Lanar DE,McLeod R

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  • A qualitative study of vaccination behaviour amongst female Polish migrants in Edinburgh, Scotland.

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    authors: Gorman DR,Bielecki K,Willocks LJ,Pollock KG

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  • RiVax, a recombinant ricin subunit vaccine, protects mice against ricin delivered by gavage or aerosol.

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  • The epidemiology and burden of rotavirus in China: a review of the literature from 1983 to 2005.

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    authors: Orenstein EW,Fang ZY,Xu J,Liu C,Shen K,Qian Y,Jiang B,Kilgore PE,Glass RI

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  • Pediatric immunization-related safety incidents in primary care: A mixed methods analysis of a national database.

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  • A novel therapeutic fusion protein vaccine by two different families of heat shock proteins linked with HPV16 E7 generates potent antitumor immunity and antiangiogenesis.

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    authors: Liu B,Ye D,Song X,Zhao X,Yi L,Song J,Zhang Z,Zhao Q

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  • Manipulation of neuraminidase packaging signals and hemagglutinin residues improves the growth of A/Anhui/1/2013 (H7N9) influenza vaccine virus yield in eggs.

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    pub_type: 杂志文章,随机对照试验


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    pub_type: 杂志文章


    authors: Dib F,Mayaud P,Launay O,Chauvin P,FSQD-HPVH Study Group.

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  • Delivery of the Pertactin/P.69 polypeptide of Bordetella pertussis using an attenuated Salmonella typhimurium vaccine strain: expression levels and immune response.

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    pub_type: 杂志文章


    authors: Anderson R,Dougan G,Roberts M

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  • Expression of cytokine genes following pre- and post-hatch immunization of chickens with herpesvirus of turkeys.

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    pub_type: 杂志文章


    authors: Abdul-Careem MF,Hunter DB,Lambourne MD,Read LR,Parvizi P,Sharif S

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    pub_type: 杂志文章


    authors: Zheng B,Lu S,Tong Q,Kong Q,Lou D

    更新日期:2013-09-23 00:00:00