Abstract:
:In this study, we tested the hypothesis that adoptive transfer of dendritic cells (DCs) transfected ex vivo with mRNA encoding hepatitis C virus (HCV) NS3/4A would initiate potent HCV-specific protective immune responses in vivo. Murine DCs were transfected with NS3/4A mRNA or eGFP mRNA using either electroporation or Transmessenger Transfection Reagent and then used for adoptive transfer. Electroporation resulted in higher transfection efficiency but lower levels of eGFP and NS3/4A expression when compared to transfection with Transmessenger. The murine NS3/4A mRNA-transfected DCs were functional in T cell activation in vitro. Adoptive transfer of NS3/4A mRNA-transfected DCs resulted in migration to regional lymph nodes, strong cellular immune responses and protection from challenge with vaccinia virus expressing NS3/NS4/NS5 in mice. Furthermore, although Transmessenger mediated transfection was less efficient than electroporation in terms of number of transfected cells, the DCs transfected with NS3/4A mRNA and Transmessenger expressed higher levels of protein and induced stronger immune responses and protection than DCs transfected with NS3/4A mRNA by electroporation. Since no study has explored the in vivo efficacy of mRNA-transfected DC-mediated vaccination against viral diseases, including hepatitis C, our study provided a novel vaccination strategy against hepatitis C as well as other pathogens.
journal_name
Vaccinejournal_title
Vaccineauthors
Yu H,Babiuk LA,van Drunen Littel-van den Hurk Sdoi
10.1016/j.vaccine.2006.11.046subject
Has Abstractpub_date
2007-02-26 00:00:00pages
1701-11issue
10eissn
0264-410Xissn
1873-2518pii
S0264-410X(06)01227-8journal_volume
25pub_type
杂志文章相关文献
VACCINE文献大全abstract:INTRODUCTION:Cellular and humoral immune responses are both involved in protection against Plasmodium infections. The only malaria vaccine available, RTS,S, primarily induces short-lived antibodies and targets only a pre-erythrocytic stage antigen. Inclusion of erythrocytic stage targets and enhancing cellular immunoge...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.03.091
更新日期:2018-05-11 00:00:00
abstract::Domestic ducks remain a major source of zoonotic Salmonella enterica infections for man worldwide and approaches to protection should include vaccine-mediated immunity. With this in mind we developed several genetically defined mutants in a virulent duck Salmonella typhimurium isolate TT-1. From initial tests for viru...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.09.004
更新日期:2015-10-13 00:00:00
abstract:AIM:To investigate parents' views regarding potential new vaccines. We examined attitudes towards severity of specific infections, acceptability of potential vaccines and preferences for the number of injections they would want their child to receive on any one occasion. DESIGN:Cross sectional survey. METHODS:Parents...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.08.057
更新日期:2007-11-07 00:00:00
abstract::In accordance with the end game strategies for polio eradication a synchronized switch plan from tOPV to bOPV was implemented globally in 2016. The National Committee for Polio Eradication (NCCPE) validated the switch activities in India. An expert group of 104 academics conducted field visits in 25 states and 2 Union...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.02.038
更新日期:2019-04-17 00:00:00
abstract::Human papillomavirus (HPV) vaccination is now offered to adolescent girls in the UK. Adolescents over 16 years old are likely to make their own decision about the vaccination. The purpose of this cross-sectional study was to assess acceptability of HPV vaccination among female adolescents (16 -- 19 years) and investig...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.02.057
更新日期:2009-04-21 00:00:00
abstract::Adjuvants are substances that enhance adaptive immune response to antigen. Development of a safe and effective immunostimulant adjuvant is essential for the efficacy of a vaccine to protect against infectious pathogens. Purple non-sulfur photosynthetic bacteria exhibited nontoxic natural lipid A variants that are dist...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.04.097
更新日期:2019-06-06 00:00:00
abstract::A growing number of people use the Internet to obtain health information, including information about vaccines. Websites that allow and promote interaction among users are an increasingly popular source of health information. Users of such so-called Web 2.0 applications (e.g. social media), while still in the minority...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.02.025
更新日期:2012-05-28 00:00:00
abstract::The case-centered approach (CCA) can be useful in vaccine safety studies to adjust for time varying confounders, especially seasonality. The method compares the observed odds of vaccination in the time interval prior to the event with the expected odds of vaccination during this interval. The log of the expected odds ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.07.064
更新日期:2013-01-02 00:00:00
abstract::We have examined the efficacy of nucleic acid vaccination in inducing immunity to the multicellular parasite, Schistosoma japonicum, a trematode worm responsible for causing schistosomiasis in humans and other mammalian species. A panel of Schistosoma japonicum cDNAs were cloned into eukaryotic expression vectors, inj...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(96)00258-7
更新日期:1997-06-01 00:00:00
abstract::Surface proteins from several different life-cycle stages of the malaria parasite Plasmodium falciparum were expressed at high levels in the yeast Saccharomyces cerevisiae. Purified proteins, both individually and in cocktails, were used to immunize mice and goats in conjunction with either Freund's adjuvant or a mura...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(93)90287-8
更新日期:1993-01-01 00:00:00
abstract::Extending the benefits of vaccination to everyone who is eligible requires an understanding of which populations current vaccination efforts have struggled to reach. A clear definition of "hard-to-reach" populations - also known as high-risk or marginalized populations, or reaching the last mile - is essential for est...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2019.06.081
更新日期:2019-09-03 00:00:00
abstract:BACKGROUND:Vaccination coverage in Bosnia and Herzegovina has been declining over recent years. A World Health Organization Tailoring Immunization Programmes (TIP) project is underway to gain insights into the underlying reasons for this, to develop tailored interventions. As part of TIP, this study aimed to investigat...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.01.025
更新日期:2020-02-18 00:00:00
abstract::The prevalence of hepatitis B virus (HBV) infection and its sequelae vary widely in the different populations living in the Asian-Pacific region. Horizontal transmission plays an important role in the spread of the disease; in East Asia, however, maternal transmission is particularly significant since a high portion o...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(90)90227-d
更新日期:1990-03-01 00:00:00
abstract::Equine influenza is a contagious diseases caused by equine influenza viruses which belong to the orthomyxovirus family. Outbreaks of equine influenza cause severe economic loses to the horse industry and consequently competition horses are required to be regularly vaccinated against equine influenza. Currently availab...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.06.085
更新日期:2009-09-04 00:00:00
abstract::Recipients of renal transplants were vaccinated with 20 ng hepatitis B surface antigen aluminium-adsorbed vaccine in order to gauge their resistance to hepatitis B infection, to which these patients are at high risk. The patients were given three 40 micrograms doses of the vaccine over a period of six months and their...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0264-410x(84)90039-2
更新日期:1984-12-01 00:00:00
abstract::Iscoms, with rCTB incorporated via the GM1 receptor, enhanced in mice the mucosal immunogenicity of rCTB as antigen after intranasal (i.n.) administration both by inducing IgA response in the remote intestinal tract mucosa and by a 100-fold increase of the specific IgA locally in the lungs. Iscom-matrix as a separate ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(99)00052-3
更新日期:1999-06-04 00:00:00
abstract:BACKGROUND:Combination antiretroviral therapy (cART) is the main therapeutic management tool for HIV/AIDS. Despite its success in controlling viral load and disease progression, cART is expensive, associated with a range of significant side effects and depends for its efficacy on the patient's life-long commitment to h...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.vaccine.2013.09.057
更新日期:2013-11-19 00:00:00
abstract:BACKGROUND:Evidence that respiratory infections trigger stroke suggests that influenza or pneumococcal vaccination might prevent stroke. We aimed to investigate whether influenza or pneumococcal vaccination or both together were associated with reduced risk of stroke or transient ischaemic attack (TIA). METHODS:We use...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.01.029
更新日期:2014-03-10 00:00:00
abstract:INTRODUCTION:During the 2018-2019 influenza season, vaccination coverage among U.S. children was 62.6%. The purpose of this study was to estimate the prevalence of influenza vaccinations among pediatric patients seen in U.S. health centers, and to explore potential disparities in vaccination coverage among subpopulatio...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.07.021
更新日期:2020-09-03 00:00:00
abstract::A randomized, double-blind, controlled clinical trial was conducted to evaluate the efficacy and safety of CJ-50300, a newly developed cell culture-derived smallpox vaccine, and to determine its minimum effective dose. The overall rates of cutaneous "take" reaction and humoral and cellular immunogenicity in CJ-50300 v...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2010.06.063
更新日期:2010-08-16 00:00:00
abstract::In Portugal, the 7-valent pneumococcal conjugate vaccine (PCV7) was not introduced in the national immunization plan but was commercially available between 2001 and 2010. We studied serotype distribution and antibiotic susceptibility of Streptococcus pneumoniae carried by children in 2009 and 2010. Vaccination with PC...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.02.045
更新日期:2016-03-29 00:00:00
abstract::The conventional treatment for paracoccidioidomycosis, the most prevalent mycosis in Latin America, involves long periods of therapy resulting in sequels and high frequency of relapses. The search for new alternatives of treatment is necessary. Previously, we have demonstrated that the hsp65 gene from Mycobacterium le...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.11.062
更新日期:2010-02-10 00:00:00
abstract::The potential of genetically fusing recombinant proteins to the simian immunodeficiency virus (SIV) Tat protein has been investigated. The recombinant SIV Tat protein was initially expressed in very low amounts in E. coli, but optimization of the coding sequence for translation in the bacterial host significantly impr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.09.012
更新日期:2006-02-06 00:00:00
abstract::The highly pathogenic avian influenza (HPAI) H5N1 viruses and their spillover into the human population pose substantial economic and public health threats. Although antiviral drugs have some effect in treating influenza infection, vaccination is still the most effective intervention to prevent possible pandemic outbr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.01.051
更新日期:2020-02-28 00:00:00
abstract::Various peptide-based approaches to simultaneous induction of multiple cytotoxic T lymphocyte (CTL) responses were evaluated as part of ongoing efforts to develop immunotherapeutic vaccines for use in humans. To this end, HLA (human histocompatibility leukocyte antigen)-A2-restricted epitopes from several specific vir...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(97)00264-8
更新日期:1998-05-01 00:00:00
abstract:BACKGROUND:The study was designed to determine the feasibility of implementing routine dTpa vaccination in the maternity ward to new mothers and to assess midwives' attitudes toward pertussis booster vaccination, their perceived susceptibility and severity of pertussis in their patients' communities, the perceived barr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.05.049
更新日期:2011-08-05 00:00:00
abstract::Sporozoite vaccination of both humans and rodents elicits potent anti-malarial immunity, but the dose of sporozoites and the number of immunizations required varies with vaccination approach. Here we examine the immunological basis for superior protection afforded from single-dose vaccination with virulent sporozoites...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.11.058
更新日期:2014-01-16 00:00:00
abstract::We produced a Vero cell-derived inactivated Japanese encephalitis vaccine using a serum-free medium, as a substitute for the conventional mouse brain-derived Japanese encephalitis vaccine. The immunogenicity of this cell-derived vaccine was higher than that of the conventional mouse brain-derived vaccine. The results ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.12.038
更新日期:2011-02-24 00:00:00
abstract:BACKGROUND:Staphylococcus aureus is a pathogen which causes life-threatening infection, the incidence of which rises during adult life. This, together with the emergence of drug-resistant strains and the expansion of more susceptible elderly populations, represents the rationale for the ongoing development of S. aureus...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.02.034
更新日期:2016-04-04 00:00:00
abstract::A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in a...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.01.055
更新日期:2007-05-04 00:00:00