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Assessment of epidermal cell viability by near infrared multi-photon microscopy following ballistic delivery of gold micro-particles.

Abstract：

:The use of gene guns in ballistically delivering DNA vaccine coated gold micro-particles to skin can potentially damage targeted cells, therefore influencing transfection efficiencies. In this paper, we assess cell death in the viable epidermis by non-invasive near infrared two-photon microscopy following micro-particle bombardment of murine skin. We show that the ballistic delivery of micro-particles to the viable epidermis can result in localised cell death. Furthermore, experimental results show the degree of cell death is dependant on the number of micro-particles delivered per unit of tissue surface area. Micro-particles densities of 0.16+/-0.27 (mean+/-S.D.), 1.35+/-0.285 and 2.72+/-0.47 per 1000 microm(2) resulted in percent deaths of 3.96+/-5.22, 45.91+/-10.89, 90.52+/-12.28, respectively. These results suggest that optimization of transfection by genes administered with gene guns is - among other effects - a compromise of micro-particle payload and cell death.

journal_name

Vaccine

journal_title

Vaccine

authors

Raju PA,McSloy N,Truong NK,Kendall MA

doi

10.1016/j.vaccine.2005.08.067

subject

Has Abstract

pub_date

2006-05-22 00:00:00

pages

4644-7

issue

21

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(05)00839-X

journal_volume

24

pub_type

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