New cloning vectors with temperature-sensitive replication.

abstract：:Sequence analysis of the Pseudomonas aeruginosa insertion sequence element IS222 revealed it to be 1234 bp in size with 23 bp imperfect terminal inverted repeats. Insertion caused a 5-bp duplication of the insertion site. Two ORFs were identified, one of which, ORFA, could encode a basic (pI 10.5) polypeptide with a m...

journal_title：Plasmid

authors： Kropinski AM,Farinha MA,Jansons I

更新日期：1994-03-01 00:00:00

abstract：:Despite the prevalence of Arthrobacter in the environment little is known about their plasmids, or the capacity of Arthrobacter plasmids to mediate horizontal gene transfer. In this study, we compared eight plasmids from five Arthrobacter strains in order to identify putative core maintenance genes for replication, se...

journal_title：Plasmid

authors： Jerke K,Nakatsu CH,Beasley F,Konopka A

更新日期：2008-03-01 00:00:00

abstract：:pSM19035 is a low-copy-number theta-replicating plasmid, which belongs to the Inc18 family. Plasmids of this family, which show a modular organization, are functional in evolutionarily diverse bacterial species of the Firmicutes Phylum. This review summarizes our understanding, accumulated during the last 20 years, on...

journal_title：Plasmid

authors： Lioy VS,Pratto F,de la Hoz AB,Ayora S,Alonso JC

更新日期：2010-07-01 00:00:00

abstract：:We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb E...

journal_title：Plasmid

authors： Kawaguchi R,Nagaoka T,Burgess JG,Takeyama H,Matsunaga T

更新日期：1994-11-01 00:00:00

abstract：:The structural and functional properties of mercury resistance determinants cloned from a series of independently isolated conjugative plasmids were compared with those of the prototype HgR determinants from Tn501 and plasmid R100 (containing Tn21). Restriction endonuclease mapping classified the HgR determinants into...

journal_title：Plasmid

authors： Jobling MG,Peters SE,Ritchie DA

更新日期：1988-09-01 00:00:00

abstract：:Transcription of the 7.5-kb cryptic plasmid of Chlamydia trachomatis serovar L2 was investigated. Faint, diffuse transcripts of about 1.6 and 2.2 kb and intense short transcripts of about 250 and 430 bases were identified by Northern blot analysis. The short transcripts were found to have a common 5' end corresponding...

journal_title：Plasmid

authors： Fahr MJ,Sriprakash KS,Hatch TP

更新日期：1992-11-01 00:00:00

abstract：:Bacterial chromosomes segregate via a partition apparatus that employs a score of specialized proteins. The SMC complexes play a crucial role in the chromosome partitioning process by organizing bacterial chromosomes through their ATP-dependent chromatin-compacting activity. Recent progress in the composition of these...

journal_title：Plasmid

authors： Strunnikov AV

更新日期：2006-03-01 00:00:00

abstract：:Coupling sequences are the 6 bp flanking the conjugative transposon Tn916 and are thought to play a role in determining the frequency of conjugative transposition. The affinity of binding of a chimeric protein, which consisted of maltose binding protein fused to the carboxy-terminal DNA binding domain of Tn916 integra...

journal_title：Plasmid

authors： Pethel B,Churchward G

更新日期：2000-03-01 00:00:00

abstract：:In order to study the in vivo assembly of chromatin on prokaryotic DNA templates, we have transformed yeast cells with plasmids pAJ50 and pRB58, which contain pBR322 sequences. In both cases nucleosomes are assembled in vivo on pBR322 DNA, although the nucleosomes are not homogeneous in size. To explore whether there ...

journal_title：Plasmid

authors： Estruch F,Pérez-Ortín JE,Matallana E,Franco L

更新日期：1989-03-01 00:00:00

abstract：:The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...

journal_title：Plasmid

authors： Bartosik D,Baj J,Piechucka E,Waker E,Wlodarczyk M

更新日期：2002-09-01 00:00:00

abstract：:A chimeric plasmid, pRS7Rep (6.1 kb), was constructed using the replication region of pRS7, a large plasmid from Oenococcus oeni, and pEM64, a plasmid derived from pIJ2925 and containing a gene for resistance to chloramphenicol. pRS7Rep is a shuttle vector that replicates in Escherichia coli using its pIJ2925 componen...

journal_title：Plasmid

authors： Rodríguez MC,Alegre MT,Martín MC,Mesas JM

更新日期：2015-01-01 00:00:00

abstract：:To study the transcriptional activity of the HIV-1 LTR, we constructed a vector containing Renilla and Firefly luciferase genes under the control of the LTR (wild-type or mutated version) and oriented in a manner that allowed them to be transcribed in opposite directions. We found that the HIV-1 LTR acted as a bidirec...

journal_title：Plasmid

authors： Arpin-André C,Laverdure S,Barbeau B,Gross A,Mesnard JM

更新日期：2014-07-01 00:00:00

abstract：:With the mutagenesis of specific, virulence-associated genes of Legionella pneumophila as the eventual goal, methods for gene transfer to these bacteria were developed. Following the observations of others that conjugative, broad-host-range plasmids could be transferred from Escherichia coli to L. pneumophila at low f...

journal_title：Plasmid

authors： Engleberg NC,Cianciotto N,Smith J,Eisenstein BI

更新日期：1988-07-01 00:00:00

abstract：:The sequences of three transfer RNAs from mosquito cell mitochondria, tRNAArgUCG, tRNAAspGUC, and tRNAIleGAU, determined using a combination of rapid ladder and fingerprinting procedures are reported. These were compared with hamster mitochondrial tRNAArgUCG and tRNAAspGUC determined similarly, and a bovine mitochondr...

journal_title：Plasmid

authors： HsuChen CC,Cleaves GR,Dubin DT

更新日期：1983-07-01 00:00:00

abstract：:A simple and reliable method for the determination of plasmid copy numbers by direct fluorescence densitometry of ethidium bromide-stained electrophoretic gels was developed. In developing the method, the following parameters were evaluated and controlled: plasmid DNA trapping in the linear chromosomal DNA, staining-d...

journal_title：Plasmid

authors： Projan SJ,Carleton S,Novick RP

更新日期：1983-03-01 00:00:00

abstract：:The imidazole and triazole fungicides inhibit cytochrome P450 14 alpha-lanosterol demethylase (P45014DM) implicated in the ergosterol biosynthesis pathway, which is specific to fungi and yeasts. Two plasmids were obtained which allow triazole and imidazole resistance in Saccharomyces cerevisiae. The low copy number pl...

journal_title：Plasmid

authors： Doignon F,Aigle M,Ribereau-Gayon P

更新日期：1993-11-01 00:00:00

abstract：:The nucleotide sequence of a smallest cryptic plasmid pRK10 of Serratia marcescens ACE2 was determined. When compared to the all other plasmids reported so far from S. marcescens in sizes of over 70kb, pRK10 is only 4241bp long with 53% G+C content and has five coding sequences representing a coding percentage of 65.4...

journal_title：Plasmid

authors： Syed Ibrahim K,Bakkiyaraj D,James R,Ganesh Babu T,Karutha Pandian ST

更新日期：2009-11-01 00:00:00

abstract：:Aeromonas hydrophila strain AO1 isolated from an infected fish was found to be resistant to several quinolones. A plasmid isolated from the strain AO1, termed pBRST7.6, was cloned and sequenced and shown to be 7621bp in length with a GC content of 60%. Further analysis confirmed that it contained a gene with 100% iden...

journal_title：Plasmid

authors： Majumdar T,Das B,Bhadra RK,Dam B,Mazumder S

更新日期：2011-07-01 00:00:00

abstract：:Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons. Amplification products were obtained from 20 of the hybridization-positive strains. A single dfrA7 cassette was identified in 18 of the isolates and an integron with two cassettes (aadB-aadA4) ...

journal_title：Plasmid

authors： Segal H,Thomas R,Gay Elisha B

更新日期：2003-03-01 00:00:00

abstract：:As part of a study identifying plasmids in Campylobacter, we isolated and sequenced two novel cryptic plasmids from an agricultural isolate of Campylobacter coli. The larger of the two plasmids, p3384, is 3316 bp in length and has a G+C content of 31.18%. A typical origin of replication consisting of five iterons was ...

journal_title：Plasmid

authors： Jesse TW,Pittenger-Alley LG,Englen MD

更新日期：2006-01-01 00:00:00

abstract：:A preliminary screening study of six Moraxella catarrhalis isolates from primary school children in the Netherlands identified a small 3.5 kb plasmid (pEMCJH03), containing four open reading frames, which encoded three mobilizing and one replicase protein. Insertion of a kanamycin containing transposon (yielding pEMCJ...

journal_title：Plasmid

authors： Hays JP,Eadie K,Verduin CM,Verbrugh H,van Belkum A

更新日期：2005-05-01 00:00:00

abstract：:An RP4-oriT shuttle vector pJIR1457 originally developed for Clostridium perfringens was successfully transferred by conjugation from Escherichia coli to Clostridium botulinum type A strains and to a nontoxigenic C. botulinum type A-transposon Tn916 mutant strain lacking the entire toxin gene cluster. The light chain ...

journal_title：Plasmid

authors： Bradshaw M,Goodnough MC,Johnson EA

更新日期：1998-11-01 00:00:00

abstract：:A 102-kb plasmid, pIJB1, was isolated from Burkholderia cepacia strain 2a, which is able to use 2,4-dichlorophenoxyacetate (2,4-D) as a sole carbon source, and a physical map of the plasmid has been established. It was observed that spontaneous loss of a 37-kb fragment of the plasmid after growth in nonselective mediu...

journal_title：Plasmid

authors： Xia XS,Aathithan S,Oswiecimska K,Smith AR,Bruce IJ

更新日期：1998-01-01 00:00:00

abstract：:The region responsible for the maintenance of the prophage of bacteriophage P7 as a stable, unit-copy plasmid was isolated in a lambda att vector which lysogenizes Escherichia coli as a stable unit-copy plasmid under the control of the P7 replication origin. The P7 plasmid-maintenance region was shown to consist of ad...

journal_title：Plasmid

authors： Ludtke DN,Austin SJ

更新日期：1987-07-01 00:00:00

abstract：:The complete nucleotide sequence of a plasmid, designated pRJF1, isolated from a rumen bacterium of the genus Butyrivibrio, has been determined. pRJF1 is a small plasmid (2631 bp) which shows the high AT content (64%) typical of plasmids from gram-positive organisms. Computer analysis of sequence data revealed two maj...

journal_title：Plasmid

authors： Hefford MA,Teather RM,Forster RJ

更新日期：1993-01-01 00:00:00

abstract：:The galactose operon of Escherichia coli K-12 has been used as a phenotypic marker for miniplasmids derived in vitro from R plasmids representing six incompatibility groups. This has enabled the development of a rapid incompatibility typing scheme in which the miniplasmids are used as incompatibility exemplars, their ...

journal_title：Plasmid

authors： Davey RB,Bird PI,Nikoletti SM,Praszkier J,Pittard J

更新日期：1984-05-01 00:00:00

abstract：:Horizontal transfer of genes encoding phenol degradation (pheBA) in the environment has been previously described. Complete or partial phe-operon was redetected in plasmids of several indigenous Pseudomonas strains isolated from the river water. The sequences of up- and downstream regions of the acquired phe-DNA in ei...

journal_title：Plasmid

authors： Peters M,Tomikas A,Nurk A

更新日期：2004-11-01 00:00:00

abstract：:Two independently isolated temperature-sensitive bacteriophage that are specific for enterobacterial hosts harboring HI and HII plasmids were characterized to determine if any identifiable differences existed between them. The traits examined included adsorption pattern of phage to H pili, bacteriophage size, sensitiv...

journal_title：Plasmid

authors： Maher D,Sherburne R,Taylor DE

更新日期：1991-09-01 00:00:00

abstract：:pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper s...

journal_title：Plasmid

authors： Leclercq J,Szabolcs T,Martin F,Montoro P

更新日期：2015-09-01 00:00:00

abstract：:A variant of the cryptic plasmid of Neisseria gonorrhoeae, 4.4 kb in size, was isolated and characterized at the molecular level. This variant harbored a 156-bp insertion which was located between coordinates 3134 and 3135 within the putative cppB gene using the 4.2-kb cryptic plasmid, pJD1, as a reference. The insert...

journal_title：Plasmid

authors： Roy RN,Bigelow N,Dillon JA

更新日期：1988-01-01 00:00:00