Comparative characterization of repABC-type replicons of Paracoccus pantotrophus composite plasmids.

abstract：:Rolling circle-replicating plasmids constitute a vast family that is particularly abundant in, but not exclusive of, Gram-positive bacteria. These plasmids are constructed as cassettes that harbor genes involved in replication and its control, mobilization, resistance determinants and one or two origins of lagging str...

journal_title：Plasmid

authors： Lorenzo-Díaz F,Fernández-López C,Garcillán-Barcia MP,Espinosa M

更新日期：2014-07-01 00:00:00

abstract：:Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons. Amplification products were obtained from 20 of the hybridization-positive strains. A single dfrA7 cassette was identified in 18 of the isolates and an integron with two cassettes (aadB-aadA4) ...

journal_title：Plasmid

authors： Segal H,Thomas R,Gay Elisha B

更新日期：2003-03-01 00:00:00

abstract：:pSAM2 is an 11-kb plasmid integrated in the Streptomyces ambofaciens ATCC23877 and ATCC15154 genomes and found additionally as a free replicon in an uv derivative. After transfer into S. ambofaciens DSM40697 (devoid of pSAM2) or into Streptomyces lividans, specific integration of pSAM2 occurred very efficiently. A 58-...

journal_title：Plasmid

authors： Boccard F,Smokvina T,Pernodet JL,Friedmann A,Guérineau M

更新日期：1989-01-01 00:00:00

abstract：:A small cryptic plasmid designated pHW15 was isolated from Rahnella genomospecies 2 WMR15 and its complete nucleotide sequence was determined. The plasmid contained 3002 bp with a G+C content of 47.4%. The origin of replication was identified by deletion analysis as a region of about 600 bp. This region had an identit...

journal_title：Plasmid

authors： Rozhon WM,Petutschnig EK,Jonak C

更新日期：2006-11-01 00:00:00

abstract：:A simple mathematical model of drug-induced plasmid elimination (curing) considering density-dependent growth rates and plasmid transfers is presented. It describes nonlinear population dynamics of conjugative plasmids during in vitro curing experiments in batch culture. The model was tested on kinetics of acridine or...

journal_title：Plasmid

authors： Sýkora P,Foltýnova Z,Smitálova K

更新日期：1989-03-01 00:00:00

abstract：:As part of a study identifying plasmids in Campylobacter, we isolated and sequenced two novel cryptic plasmids from an agricultural isolate of Campylobacter coli. The larger of the two plasmids, p3384, is 3316 bp in length and has a G+C content of 31.18%. A typical origin of replication consisting of five iterons was ...

journal_title：Plasmid

authors： Jesse TW,Pittenger-Alley LG,Englen MD

更新日期：2006-01-01 00:00:00

abstract：:A putative insertion sequence (IS1473) has been cloned and sequenced. The 1087-bp element was found between the moaA and the ndhA genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pAO1 plasmid of Arthrobacter nicotinovorans. It is flanked by an imperfect repeat of 33 bp and carries ...

journal_title：Plasmid

authors： Menéndez C,Igloi GL,Brandsch R

更新日期：1997-01-01 00:00:00

abstract：:A variant of the cryptic plasmid of Neisseria gonorrhoeae, 4.4 kb in size, was isolated and characterized at the molecular level. This variant harbored a 156-bp insertion which was located between coordinates 3134 and 3135 within the putative cppB gene using the 4.2-kb cryptic plasmid, pJD1, as a reference. The insert...

journal_title：Plasmid

authors： Roy RN,Bigelow N,Dillon JA

更新日期：1988-01-01 00:00:00

abstract：:Ten strains of Rhodopseudomonas sphaeroides were analyzed by agarose gel electrophoresis for plasmid DNA content and, by filter-hybridizations, for their molecular relationships. All strains examined contained at least one plasmid. Several strains carried as many as six different plasmid species with sizes ranging fro...

journal_title：Plasmid

authors： Fornari CS,Watkins M,Kaplan S

更新日期：1984-01-01 00:00:00

abstract：:Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurring compatible members of the Dictyostelium Ddp1 plasmid family found in the same wild isolate strain. The nucleotide sequences of Dmp1 (5983 bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading frames (ORFs) similar to the G1 and G5...

journal_title：Plasmid

authors： Kiyosawa H,Hughes JE,Welker DL

更新日期：1994-03-01 00:00:00

abstract：:The nucleotide sequence of two novel plasmids isolated from the extreme thermophilic anaerobic bacterium Anaerocellum thermophilum DSM6725 (A. thermophilum), growing optimally at 70 degrees C, has been determined. pBAS2 was found to be a 3653 bp plasmid with a GC content of 43%, and the sequence revealed 10 open readi...

journal_title：Plasmid

authors： Clausen A,Mikkelsen MJ,Schröder I,Ahring BK

更新日期：2004-09-01 00:00:00

abstract：:The Tra1 region of RP1 from a derivative with Tn7 inserted into the kanamycin resistance determinant was cloned, using EcoRI, into the multicopy vector plasmid pBR325. For one orientation of the cloned fragment the resultant chimeric plasmid was very frequently lost from the cell, but in the other orientation it was m...

journal_title：Plasmid

authors： Watson J,Schmidt L,Willetts N

更新日期：1980-09-01 00:00:00

abstract：:Escherichia coli strain K12 BM14, carrying plasmid R751 (51.4 kb; Tpr, Tra+, IncP), was used as donor in matings with reference strains of the six Brucella nomenspecies, not known so far to harbor naturally occurring plasmids. R751 was easily transferred to and between Brucella spp., at frequencies ranging between 10(...

journal_title：Plasmid

authors： Verger JM,Grayon M,Chaslus-Dancla E,Meurisse M,Lafont JP

更新日期：1993-03-01 00:00:00

abstract：:To study the transcriptional activity of the HIV-1 LTR, we constructed a vector containing Renilla and Firefly luciferase genes under the control of the LTR (wild-type or mutated version) and oriented in a manner that allowed them to be transcribed in opposite directions. We found that the HIV-1 LTR acted as a bidirec...

journal_title：Plasmid

authors： Arpin-André C,Laverdure S,Barbeau B,Gross A,Mesnard JM

更新日期：2014-07-01 00:00:00

abstract：:In order to study the in vivo assembly of chromatin on prokaryotic DNA templates, we have transformed yeast cells with plasmids pAJ50 and pRB58, which contain pBR322 sequences. In both cases nucleosomes are assembled in vivo on pBR322 DNA, although the nucleosomes are not homogeneous in size. To explore whether there ...

journal_title：Plasmid

authors： Estruch F,Pérez-Ortín JE,Matallana E,Franco L

更新日期：1989-03-01 00:00:00

abstract：:Previous explanations of why bacterial genes for certain "optional" traits tend to occur on plasmids rather than chromosomes are based on an outdated misunderstanding of natural selection. They also fail to explain why certain characters that are ubiquitous in some bacterial species tend to occur on plasmids. This pap...

journal_title：Plasmid

authors： Eberhard WG

更新日期：1989-05-01 00:00:00

abstract：:The region responsible for the maintenance of the prophage of bacteriophage P7 as a stable, unit-copy plasmid was isolated in a lambda att vector which lysogenizes Escherichia coli as a stable unit-copy plasmid under the control of the P7 replication origin. The P7 plasmid-maintenance region was shown to consist of ad...

journal_title：Plasmid

authors： Ludtke DN,Austin SJ

更新日期：1987-07-01 00:00:00

abstract：:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...

journal_title：Plasmid

authors： Taylor DE,Brose EC

更新日期：1985-01-01 00:00:00

abstract：:Pseudomonas putida is a highly attractive production system for industrial needs. However, for its improvement as a biocatalyst at the industrial level, modulation of its gene expression is urgently needed. We report the construction of a plasmid expressing a small RNA-based system with the potential to be used for di...

journal_title：Plasmid

authors： Apura P,Saramago M,Peregrina A,Viegas SC,Carvalho SM,Saraiva LM,Arraiano CM,Domingues S

更新日期：2020-05-01 00:00:00

abstract：:To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...

journal_title：Plasmid

authors： Domingues E,Brillet T,Vasseur C,Agier V,Marden MC,Baudin-Creuza V

更新日期：2009-01-01 00:00:00

abstract：:The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of co...

journal_title：Plasmid

authors： Caillaud F,Carlier C,Courvalin P

更新日期：1987-01-01 00:00:00

abstract：:The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155....

journal_title：Plasmid

authors： Moran RA,Anantham S,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:Even though Vibrio cholerae is a well-known human pathogen, it is also a normal member of aquatic habitats. Within this environment it often forms biofilms on the chitin-containing exoskeleton of crustaceans and their molts. Chitin not only serves as nutrient source but also induces a developmental program called natu...

journal_title：Plasmid

authors： De Souza Silva O,Blokesch M

更新日期：2010-11-01 00:00:00

abstract：:Acinetobacter baumannii is an important nosocomial pathogen that often complicates treatment because of its high level of resistance to antibiotics. Though plasmids can potentially introduce various genes into bacterial strains, compared to other Gram-negative bacteria, information about the unique A. baumannii plasmi...

journal_title：Plasmid

authors： Hamidian M,Ambrose SJ,Hall RM

更新日期：2016-01-01 00:00:00

abstract：:pBTK445 is a newly described large (∼60Kb), low-copy number, conjugative plasmid indigenous to the sulfur-chemolithoautotroph Advenella kashmirensis. Based on its minimal replication region, a shuttle vector, pBTKS was constructed which can be used for diverse Alcaligenaceae members. The construct was found to be stab...

journal_title：Plasmid

authors： Dam B

更新日期：2011-03-01 00:00:00

abstract：:pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper s...

journal_title：Plasmid

authors： Leclercq J,Szabolcs T,Martin F,Montoro P

更新日期：2015-09-01 00:00:00

abstract：:Temperate bacteriophage MAV1 is found in certain highly virulent strains of Mycoplasma arthritidis. Integration sites, portions of the right and left prophage ends, and flanking DNA from eight prophages in seven M. arthritidis strains were characterized in this study. attb and attp sites conformed for the most part to...

journal_title：Plasmid

authors： Washburn LR,Miller EJ,Mukherjee S,Dannenbring D

更新日期：2004-07-01 00:00:00

abstract：:Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have b...

journal_title：Plasmid

authors： Banerjee H,Verma M

更新日期：2000-03-01 00:00:00

abstract：:A 102-kb plasmid, pIJB1, was isolated from Burkholderia cepacia strain 2a, which is able to use 2,4-dichlorophenoxyacetate (2,4-D) as a sole carbon source, and a physical map of the plasmid has been established. It was observed that spontaneous loss of a 37-kb fragment of the plasmid after growth in nonselective mediu...

journal_title：Plasmid

authors： Xia XS,Aathithan S,Oswiecimska K,Smith AR,Bruce IJ

更新日期：1998-01-01 00:00:00

abstract：:Nonviral gene delivery has gained a lot of interest as a promising approach for gene therapy. Despite intensive studies and much progress the outcome of nonviral vectors has remained significantly weaker than that of viral vectors. A weak transfection efficiency of nonviral gene transfection is still limiting their in...

journal_title：Plasmid

authors： Soininen P,Hanzlíková M,Paukkunen M,Lecklin A,Männistö PT,Raasmaja A

更新日期：2010-01-01 00:00:00