Abstract:
:An RP4-oriT shuttle vector pJIR1457 originally developed for Clostridium perfringens was successfully transferred by conjugation from Escherichia coli to Clostridium botulinum type A strains and to a nontoxigenic C. botulinum type A-transposon Tn916 mutant strain lacking the entire toxin gene cluster. The light chain (LC) of botulinum toxin was highly expressed in the toxin deletion mutant strain from a pJIR1457 construct containing the recombinant botulinal gene for LC. This shuttle vector system will be valuable for genetic analysis of C. botulinum and will enable genetic manipulation and recombinant expression studies of botulinum neurotoxins as pharmaceutical agents.
journal_name
Plasmidjournal_title
Plasmidauthors
Bradshaw M,Goodnough MC,Johnson EAdoi
10.1006/plas.1998.1366subject
Has Abstractpub_date
1998-11-01 00:00:00pages
233-7issue
3eissn
0147-619Xissn
1095-9890pii
S0147-619X(98)91366-Xjournal_volume
40pub_type
杂志文章相关文献
PLASMID文献大全abstract::A procedure for the isolation of closed circular mitochondrial DNA (mtDNA) from cells permeabilized with digitonin is described. Compared to the standard procedure in which cells are broken after osmotic swelling, the digitonin-based procedure is more consistent and results in higher mtDNA yields. ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(86)90083-1
更新日期:1986-07-01 00:00:00
abstract::Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1289
更新日期:1997-01-01 00:00:00
abstract::Rolling circle-replicating plasmids constitute a vast family that is particularly abundant in, but not exclusive of, Gram-positive bacteria. These plasmids are constructed as cassettes that harbor genes involved in replication and its control, mobilization, resistance determinants and one or two origins of lagging str...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2014.05.004
更新日期:2014-07-01 00:00:00
abstract::Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2018.01.002
更新日期:2018-01-01 00:00:00
abstract::Analysis of the 50-kb R-plasmid pTP10 from the clinical isolate Corynebacterium xerosis M82B revealed that the erythromycin resistance gene, ermCX, is located on a 4524-bp composite transposable element, Tn5432. The ends of Tn5432 are identical, direct repeats of an insertion sequence, designated IS1249, encoding a pu...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.9995
更新日期:1995-09-01 00:00:00
abstract::Upon excision, conjugative transposon Tn916 forms a nonreplicating circular intermediate (CI), which is essential both for transposition and conjugal transfer. In this work we developed an assay to quantify the circular forms of Tn916. By this method we defined a new measurable trait of Tn916-carrying strains: the "CI...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.1032
更新日期:1995-07-01 00:00:00
abstract::As multidrug resistant bacteria pose one of the greatest risks to human health new alternative antibacterial agents are urgently needed. One possible mechanism that can be used as an alternative to traditional antibiotic therapy is transfer of killing agents via conjugation. Our work was aimed at providing a proof of ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.10.001
更新日期:2015-11-01 00:00:00
abstract::The stable maintenance of certain plasmids in bacterial populations has contributed significantly to the current worldwide antibiotic resistance (AbR) emergency. IncX plasmids, long underestimated in this regard, have achieved recent notoriety for their roles in transmission of resistance to carbapenem and colistin, t...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2017.02.006
更新日期:2017-05-01 00:00:00
abstract::The complete sequence of the 10.9-kbp bacteriocinogenic plasmid pBL1 from Lactococcus lactis subsp. lactis IPLA 972 has been determined. Thirteen ORFs were encountered, of which 5 were incomplete. pBL1 proved to be a narrow-host-range plasmid which replicates neither in Bacilus subtilis nor in Lactobacillus spp. The s...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1482
更新日期:2000-11-01 00:00:00
abstract::Single plasmids designed to express the six human type I steroid hormone receptors and detect signaling activity are described in this report. These stably replicating plasmids reported ligand-induced transcriptional activation via lacZ assays in Baker's yeast (Saccharomyces cerevisiae). The ligand concentrations need...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.11.003
更新日期:2010-03-01 00:00:00
abstract::Horizontal transfer of genes encoding phenol degradation (pheBA) in the environment has been previously described. Complete or partial phe-operon was redetected in plasmids of several indigenous Pseudomonas strains isolated from the river water. The sequences of up- and downstream regions of the acquired phe-DNA in ei...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.07.003
更新日期:2004-11-01 00:00:00
abstract::A suppressor of the mutations which inhibit the establishment of lysogeny in Pseudomonas aeruginosa has been found to be encoded by P. aeruginosa plasmids of four separate incompatibility groups. The wide distribution of this phenotype (designated Sly for suppressor of lysogeny establishment deficiency) indicates that...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(84)90024-6
更新日期:1984-03-01 00:00:00
abstract::A variant of the cryptic plasmid of Neisseria gonorrhoeae, 4.4 kb in size, was isolated and characterized at the molecular level. This variant harbored a 156-bp insertion which was located between coordinates 3134 and 3135 within the putative cppB gene using the 4.2-kb cryptic plasmid, pJD1, as a reference. The insert...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(88)90061-3
更新日期:1988-01-01 00:00:00
abstract::The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(02)00100-2
更新日期:2002-09-01 00:00:00
abstract::We have cloned and characterized an insertion sequence from Leuconostoc mesenteroides subsp. cremoris strain DB1165. This element, designated IS1165, is 1553 bp, has imperfect inverted repeat ends, contains an open reading frame of 1236 bp, and is not related to any previously described insertion sequence. The copy nu...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90022-3
更新日期:1992-05-01 00:00:00
abstract::A preliminary screening study of six Moraxella catarrhalis isolates from primary school children in the Netherlands identified a small 3.5 kb plasmid (pEMCJH03), containing four open reading frames, which encoded three mobilizing and one replicase protein. Insertion of a kanamycin containing transposon (yielding pEMCJ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.11.003
更新日期:2005-05-01 00:00:00
abstract::The incompatibility region of the Streptomyces plasmid pSL1 was identified as a 240-bp segment, though some other function from the vector plasmid was also necessary. A 540-bp fragment including the 240-bp region was enough for full expression of incompatibility. Inserted mutation analysis led to a more detailed locat...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(87)90021-7
更新日期:1987-03-01 00:00:00
abstract::The region responsible for the maintenance of the prophage of bacteriophage P7 as a stable, unit-copy plasmid was isolated in a lambda att vector which lysogenizes Escherichia coli as a stable unit-copy plasmid under the control of the P7 replication origin. The P7 plasmid-maintenance region was shown to consist of ad...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(87)90083-7
更新日期:1987-07-01 00:00:00
abstract::This review provides a historical perspective of the major findings that contributed to our current understanding of plasmid rolling-circle (RC) replication. Rolling-circle-replicating (RCR) plasmids were discovered approximately 20 years ago. The first of the RCR plasmids to be identified were native to Gram-positive...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2004.12.008
更新日期:2005-03-01 00:00:00
abstract::A small cryptic plasmid designated pHW15 was isolated from Rahnella genomospecies 2 WMR15 and its complete nucleotide sequence was determined. The plasmid contained 3002 bp with a G+C content of 47.4%. The origin of replication was identified by deletion analysis as a region of about 600 bp. This region had an identit...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2006.05.007
更新日期:2006-11-01 00:00:00
abstract::In order to study the in vivo assembly of chromatin on prokaryotic DNA templates, we have transformed yeast cells with plasmids pAJ50 and pRB58, which contain pBR322 sequences. In both cases nucleosomes are assembled in vivo on pBR322 DNA, although the nucleosomes are not homogeneous in size. To explore whether there ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(89)90054-1
更新日期:1989-03-01 00:00:00
abstract::We have examined the extent to which the degradative plasmids SAL, NAH, and TOL of the Inc P-9 incompatibility group share common DNA sequences. The homology we observe using 32P-labeled SAL and NAH DNA probes can be assigned to six regions of the TOL (pWWO) restriction endonuclease cleavage map. At least three of the...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(83)90069-0
更新日期:1983-09-01 00:00:00
abstract::Previous explanations of why bacterial genes for certain "optional" traits tend to occur on plasmids rather than chromosomes are based on an outdated misunderstanding of natural selection. They also fail to explain why certain characters that are ubiquitous in some bacterial species tend to occur on plasmids. This pap...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/0147-619x(89)90040-1
更新日期:1989-05-01 00:00:00
abstract::In the present study we report the complete nucleotide sequence of the toluene catabolic plasmid pD2RT of Pseudomonas migulae strain D2RT isolated from Baltic Sea water. The pD2RT is 129,894 base pairs in size with an average G+C content of 53.75%. A total of 135 open reading frames (ORFs) were predicted to encode pro...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2013.09.003
更新日期:2013-11-01 00:00:00
abstract::A putative insertion sequence (IS1473) has been cloned and sequenced. The 1087-bp element was found between the moaA and the ndhA genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pAO1 plasmid of Arthrobacter nicotinovorans. It is flanked by an imperfect repeat of 33 bp and carries ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.1272
更新日期:1997-01-01 00:00:00
abstract::Results of restriction endonuclease analysis and Southern blot hybridization suggest that the R-plasmids from Vibrio anguillarum strains isolated in Japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. Molecular cloning experiments allowed identif...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(84)90038-6
更新日期:1984-11-01 00:00:00
abstract::pSK41 is a prototypical 46-kb conjugative multiresistance plasmid of Staphylococcus aureus. The pSK41 replication initiation protein (Rep) is rate-limiting for plasmid replication, and its expression is negatively regulated by a small, non-coding antisense transcript, RNAI, that is complementary to the rep mRNA leader...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.01.002
更新日期:2015-03-01 00:00:00
abstract::pBTK445 is a newly described large (∼60Kb), low-copy number, conjugative plasmid indigenous to the sulfur-chemolithoautotroph Advenella kashmirensis. Based on its minimal replication region, a shuttle vector, pBTKS was constructed which can be used for diverse Alcaligenaceae members. The construct was found to be stab...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.12.007
更新日期:2011-03-01 00:00:00
abstract::Bacillus coagulans is a sporogenic lactic acid bacterium that ferments glucose and xylose, major components of plant biomass, a potential feedstock for cellulosic ethanol. The temperature and pH for optimum rate of growth of B. coagulans (50 to 55 degrees C, pH 5.0) are very similar to that of commercially developed f...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2006.11.006
更新日期:2007-07-01 00:00:00
abstract::Restriction map of the 72-kb virulence plasmid isolated from Yersinia enterocolitica O:3 was generated using EcoRI, BamHI, HindIII, and XbaI restriction enzymes. The mapping was done after cloning all of the 13 BamHI fragments of the plasmid in Escherichia coli. In addition, the restriction enzyme analysis revealed tw...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(86)90062-4
更新日期:1986-11-01 00:00:00