Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays.

abstract：:A variant of the cryptic plasmid of Neisseria gonorrhoeae, 4.4 kb in size, was isolated and characterized at the molecular level. This variant harbored a 156-bp insertion which was located between coordinates 3134 and 3135 within the putative cppB gene using the 4.2-kb cryptic plasmid, pJD1, as a reference. The insert...

journal_title：Plasmid

authors： Roy RN,Bigelow N,Dillon JA

更新日期：1988-01-01 00:00:00

abstract：:The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155....

journal_title：Plasmid

authors： Moran RA,Anantham S,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:We have examined the extent to which the degradative plasmids SAL, NAH, and TOL of the Inc P-9 incompatibility group share common DNA sequences. The homology we observe using 32P-labeled SAL and NAH DNA probes can be assigned to six regions of the TOL (pWWO) restriction endonuclease cleavage map. At least three of the...

journal_title：Plasmid

authors： Lehrbach PR,McGregor I,Ward JM,Broda P

更新日期：1983-09-01 00:00:00

abstract：:Sphingomonas sp. A1 (strain A1) is capable of directly incorporating macromolecules (e.g., alginate) through the specialized import system--"super-channel." Here, we report the complete DNA sequence and genetic organization of plasmid pA1 from strain A1. Nucleotide sequence analysis revealed that pA1 comprises 46,557 ...

journal_title：Plasmid

authors： Harada KM,Aso Y,Hashimoto W,Mikami B,Murata K

更新日期：2006-07-01 00:00:00

abstract：:Two improved Deinococcus radiodurans-Escherichia coli shuttle vectors have been constructed. pI3 is a 16-kb plasmid that confers chloramphenicol resistance in D. radiodurans (CmR, cat) and ampicillin resistance in E. coli (ApR) and contains a multiple cloning site that does not interrupt sequences necessary for replic...

journal_title：Plasmid

authors： Masters CI,Minton KW

更新日期：1992-11-01 00:00:00

abstract：:We have constructed a pUC19-based multipurpose ATG vector in Schizosaccharomyces pombe with higher copy number and mitotic stability possible with commonly used vectors. The vector, having an NdeI site in its polylinker to provide ATG site for expression, carries a greatly truncated version of URA3 gene, URA3m, of Sac...

journal_title：Plasmid

authors： Verma HK,Singh J

更新日期：2012-11-01 00:00:00

abstract：:Several staphylococcal plasmids (26-45 kb) carry all three streptogramin-resistance (Sg(R)) genes, vat, vgb, and vga. Seven such plasmids harbored by independent strains belonging to three taxa (Staphylococcus aureus, S. simulans, and S. cohnii subsp. urealyticum) were compared and the deleted derivative of one of the...

journal_title：Plasmid

authors： Allignet J,El Solh N

更新日期：1999-09-01 00:00:00

abstract：:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...

journal_title：Plasmid

authors： Yamamoto T,Hanawa T,Murayama SY,Ogata S

更新日期：1994-09-01 00:00:00

abstract：:We describe here the construction of Gateway-compatible vectors, pBGP1-DEST and pPICZα-DEST, for rapid and convenient preparation of expression plasmids for production of secretory proteins in Pichia pastoris. Both vectors direct the synthesis of fusion proteins consisting of the N-terminal signal and pro-sequences of...

journal_title：Plasmid

authors： Sasagawa T,Matsui M,Kobayashi Y,Otagiri M,Moriya S,Sakamoto Y,Ito Y,Lee CC,Kitamoto K,Arioka M

更新日期：2011-01-01 00:00:00

abstract：:A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restrictio...

journal_title：Plasmid

authors： Phillips GJ

更新日期：1999-01-01 00:00:00

abstract：:The Tra1 region of RP1 from a derivative with Tn7 inserted into the kanamycin resistance determinant was cloned, using EcoRI, into the multicopy vector plasmid pBR325. For one orientation of the cloned fragment the resultant chimeric plasmid was very frequently lost from the cell, but in the other orientation it was m...

journal_title：Plasmid

authors： Watson J,Schmidt L,Willetts N

更新日期：1980-09-01 00:00:00

abstract：:Two B/O plasmids were sequenced. The kanamycin resistance plasmid R805a was found in a Salmonella Typhi strain from a 1972 typhoid fever outbreak in Mexico City. pCERC6, which confers resistance to ampicillin, streptomycin and sulphamethoxazole, was found in a commensal E. coli isolated from a healthy adult in Sydney ...

journal_title：Plasmid

authors： Moran RA,Richardson IA,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:To study the transcriptional activity of the HIV-1 LTR, we constructed a vector containing Renilla and Firefly luciferase genes under the control of the LTR (wild-type or mutated version) and oriented in a manner that allowed them to be transcribed in opposite directions. We found that the HIV-1 LTR acted as a bidirec...

journal_title：Plasmid

authors： Arpin-André C,Laverdure S,Barbeau B,Gross A,Mesnard JM

更新日期：2014-07-01 00:00:00

abstract：:From Enterococcus faecalis cells containing random chromosomal insertions of Tn916, strains resistant to a lytic phage were selected and tested for conjugal mating ability. The phage-resistant strains all showed decreased recipient ability (Con-) in broth matings with donors carrying pheromone-inducible plasmids. Thes...

journal_title：Plasmid

authors： Trotter KM,Dunny GM

更新日期：1990-07-01 00:00:00

abstract：:An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was developed. The method relies on two integration-proficient vectors, mini-CTX1 and mini-CTX2. These two vectors contain (1) a tetracycline (tet) selectable marker, (2) an oriT for conjugation-...

journal_title：Plasmid

authors： Hoang TT,Kutchma AJ,Becher A,Schweizer HP

更新日期：2000-01-01 00:00:00

abstract：:Almost 200 scientists from America, Europe, Asia, Australia, and Africa participated in the Plasmid Biology 2004 meeting, which was organized between 15th and 21st September 2004 in Kanoni (Corfu island), Greece. Various aspects of biology of plasmids and other mobile genetic elements were discussed during the meeting...

journal_title：Plasmid

authors： Wegrzyn G

更新日期：2005-01-01 00:00:00

abstract：:The ability of some bacteria to grow in the presence of high concentrations of tellurium compounds has been recognized for almost 100 years. Since then, interest in this phenomenon has generated a slow but steady trickle of literature. In the past few years, the use of modern techniques in molecular biology has led to...

journal_title：Plasmid

authors： Walter EG,Taylor DE

更新日期：1992-01-01 00:00:00

abstract：:pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...

journal_title：Plasmid

authors： Chiang CS,Bremer H

更新日期：1991-11-01 00:00:00

abstract：:With the mutagenesis of specific, virulence-associated genes of Legionella pneumophila as the eventual goal, methods for gene transfer to these bacteria were developed. Following the observations of others that conjugative, broad-host-range plasmids could be transferred from Escherichia coli to L. pneumophila at low f...

journal_title：Plasmid

authors： Engleberg NC,Cianciotto N,Smith J,Eisenstein BI

更新日期：1988-07-01 00:00:00

abstract：:In order to study the in vivo assembly of chromatin on prokaryotic DNA templates, we have transformed yeast cells with plasmids pAJ50 and pRB58, which contain pBR322 sequences. In both cases nucleosomes are assembled in vivo on pBR322 DNA, although the nucleosomes are not homogeneous in size. To explore whether there ...

journal_title：Plasmid

authors： Estruch F,Pérez-Ortín JE,Matallana E,Franco L

更新日期：1989-03-01 00:00:00

abstract：:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...

journal_title：Plasmid

authors： Taylor DE,Brose EC

更新日期：1985-01-01 00:00:00

abstract：:The rapidly advancing field of plant synthetic biology requires transforming plants with multiple genes. This has sparked a growing interest in flexible plant transformation vectors, which can be used for multi-gene transformations. We have developed a novel binary vector series, named the PC-GW series (GenBank: KP826...

journal_title：Plasmid

authors： Dalal J,Yalamanchili R,La Hovary C,Ji M,Rodriguez-Welsh M,Aslett D,Ganapathy S,Grunden A,Sederoff H,Qu R

更新日期：2015-09-01 00:00:00

abstract：:Unequal crossing-over is involved in genetic duplication and deletion in such diverse genetic systems as Drosophila, bacteria, and animal viruses. It is proposed to be involved in the form of unequal sister chromatid exchange in gene amplification in cultured animal cells and during carcinogenesis. Studies of the proc...

journal_title：Plasmid

authors： Clevenger W,Reeves R,Pall ML

更新日期：1985-05-01 00:00:00

abstract：:In the present study we report the complete nucleotide sequence of the toluene catabolic plasmid pD2RT of Pseudomonas migulae strain D2RT isolated from Baltic Sea water. The pD2RT is 129,894 base pairs in size with an average G+C content of 53.75%. A total of 135 open reading frames (ORFs) were predicted to encode pro...

journal_title：Plasmid

authors： Jutkina J,Hansen LH,Li L,Heinaru E,Vedler E,Jõesaar M,Heinaru A

更新日期：2013-11-01 00:00:00

abstract：:To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...

journal_title：Plasmid

authors： Domingues E,Brillet T,Vasseur C,Agier V,Marden MC,Baudin-Creuza V

更新日期：2009-01-01 00:00:00

abstract：:A collection of 111 commensal Escherichia coli isolated from 84 faecal samples from healthy Australian adults were screened using PCR-based replicon typing. Each isolate represented a distinct strain found in a particular faecal sample. Fifty-one isolates were resistant to one or more of 12 antibiotics tested. FII and...

journal_title：Plasmid

authors： Moran RA,Anantham S,Pinyon JL,Hall RM

更新日期：2015-07-01 00:00:00

abstract：:It is now known that conjugative pili are determined by representative plasmids for all incompatibility groups in Escherichia coli K-12. They fall into three basic morphological groups, which are described: thin flexible, thick flexible, and rigid filaments or rods. The main thrust of this study, however, has been the...

journal_title：Plasmid

authors： Bradley DE

更新日期：1980-09-01 00:00:00

abstract：:By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sens...

journal_title：Plasmid

authors： Maas R

更新日期：1983-11-01 00:00:00

abstract：:DNA sequence polymorphism was determined for the microcin V gene cluster encoded on the microcin V plasmids of 12 natural isolates of Escherichia coli. These microcin V gene clusters are similar in DNA sequence, with only 10 of the 683 bp polymorphic. Further, the levels and patterns of microcin V gene cluster polymor...

journal_title：Plasmid

authors： Pinou T,Riley MA

更新日期：2001-07-01 00:00:00

abstract：:Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons. Amplification products were obtained from 20 of the hybridization-positive strains. A single dfrA7 cassette was identified in 18 of the isolates and an integron with two cassettes (aadB-aadA4) ...

journal_title：Plasmid

authors： Segal H,Thomas R,Gay Elisha B

更新日期：2003-03-01 00:00:00