Comparative analysis of staphylococcal plasmids carrying three streptogramin-resistance genes: vat-vgb-vga.

abstract：:We describe the construction of a series of shuttle vectors for Staphylococcus aureus. In order to determine transcriptional regulation by essential regulators, we constructed promoterless luxABCDE reporter system using a TetR-regulated antisense RNA expression vector, pJYJ909, which is composed of S. aureus plasmid p...

journal_title：Plasmid

authors： Yan M,Yu C,Yang J,Ji Y

更新日期：2009-05-01 00:00:00

abstract：:Little knowledge of DNA replication in cyanobacteria is available. In this study, we report the development and characterization of an in vitro system for studies of replication of the endogenous plasmids from the unicellular cyanobacterium Synechocystis 6803. This system (fraction III) was isolated at high salt conce...

journal_title：Plasmid

authors： Yang X,Daniell H,McFadden B

更新日期：1994-09-01 00:00:00

abstract：:To study the transcriptional activity of the HIV-1 LTR, we constructed a vector containing Renilla and Firefly luciferase genes under the control of the LTR (wild-type or mutated version) and oriented in a manner that allowed them to be transcribed in opposite directions. We found that the HIV-1 LTR acted as a bidirec...

journal_title：Plasmid

authors： Arpin-André C,Laverdure S,Barbeau B,Gross A,Mesnard JM

更新日期：2014-07-01 00:00:00

abstract：:RNA interference is a widely used tool for analysis of gene function in mammalian cells. Stable knockdown of specific target genes can be maintained in cell lines and live organisms using vector-based delivery of short hairpins (shRNAs) driven by RNA polymerase III promoters. Here we describe a vector incorporating th...

journal_title：Plasmid

authors： Sotirova VN,Calciano MA,Krueger W,Lalande M

更新日期：2006-05-01 00:00:00

abstract：:Nocardia brasiliensis, is a bacteria that lives as saprophyte in soil and causes a disease called actinomycetoma in both human and animals. Nocardia brasiliensis is an intracellular, facultative bacterium that replicates and survives within host macrophages. The mechanisms involved in the evasion of the microbicidal a...

journal_title：Plasmid

authors： Salinas-Carmona MC,Rocha-Pizaña MR

更新日期：2011-01-01 00:00:00

abstract：:Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have b...

journal_title：Plasmid

authors： Banerjee H,Verma M

更新日期：2000-03-01 00:00:00

abstract：:A simple mathematical model of drug-induced plasmid elimination (curing) considering density-dependent growth rates and plasmid transfers is presented. It describes nonlinear population dynamics of conjugative plasmids during in vitro curing experiments in batch culture. The model was tested on kinetics of acridine or...

journal_title：Plasmid

authors： Sýkora P,Foltýnova Z,Smitálova K

更新日期：1989-03-01 00:00:00

abstract：:The 4.2-kb cryptic plasmid pVA380-1 has been used as a vector for the cloning of antibiotic resistance genes directly in streptococci, and in the construction of Escherichia coli/Streptococcus shuttle vectors. The results of subcloning experiments located the basic replicon of pVA380-1 within a 2.5-kb region. The nucl...

journal_title：Plasmid

authors： LeBlanc DJ,Lee LN,Abu-Al-Jaibat A

更新日期：1992-09-01 00:00:00

abstract：:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...

journal_title：Plasmid

authors： Taylor DE,Brose EC

更新日期：1985-01-01 00:00:00

abstract：:Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...

journal_title：Plasmid

authors： D'Haeze W,Verplancke C,Mironov V,Holsters M

更新日期：2002-03-01 00:00:00

abstract：:Corynebacterium glutamicum is an industrial microorganism for production of amino acids. However, the metabolic engineering in C. glutamicum has been retarded due to lack of suitable vectors. In this study, we have constructed a shuttle vector pDXW-10 which harbors a large multiple cloning site suitable for cloning mu...

journal_title：Plasmid

authors： Xu D,Tan Y,Shi F,Wang X

更新日期：2010-09-01 00:00:00

abstract：:We have constructed shuttle vectors for integration of genes via double homologous recombination into three ectopic sites on the chromosome of Bacillus subtilis. The sites of integration are the pyrD, gltA, and sacA genes located at 139 degrees, 172 degrees, and 333 degrees, respectively, on the chromosome. Integratio...

journal_title：Plasmid

authors： Middleton R,Hofmeister A

更新日期：2004-05-01 00:00:00

abstract：:The galactose operon of Escherichia coli K-12 has been used as a phenotypic marker for miniplasmids derived in vitro from R plasmids representing six incompatibility groups. This has enabled the development of a rapid incompatibility typing scheme in which the miniplasmids are used as incompatibility exemplars, their ...

journal_title：Plasmid

authors： Davey RB,Bird PI,Nikoletti SM,Praszkier J,Pittard J

更新日期：1984-05-01 00:00:00

abstract：:Most Vibrio fischeri strains isolated from the Euprymna scolopes light organ carry plasmids, often including both a large (>40kb) plasmid, and one or more small (<12kb) plasmids. The large plasmids share homology with pES100, which is the lone plasmid in V. fischeri type strain ES114. pES100 appears to encode a conjug...

journal_title：Plasmid

authors： Dunn AK,Martin MO,Stabb EV

更新日期：2005-09-01 00:00:00

abstract：:An RP4-oriT shuttle vector pJIR1457 originally developed for Clostridium perfringens was successfully transferred by conjugation from Escherichia coli to Clostridium botulinum type A strains and to a nontoxigenic C. botulinum type A-transposon Tn916 mutant strain lacking the entire toxin gene cluster. The light chain ...

journal_title：Plasmid

authors： Bradshaw M,Goodnough MC,Johnson EA

更新日期：1998-11-01 00:00:00

abstract：:A plasmid vector for cloning in Bacillus sphaericus 1593 was constructed in B. subtilis from two parent plasmids, pBC16 and pBD64. When characterized, the 3.9-MDa chimeric plasmid pNN101 was found to consist of the MspI fragment containing the chloramphenicol acetyltransferase (CAT) gene from pBD64 inserted into an Ms...

journal_title：Plasmid

authors： Norton NB,Orzech KA,Burke WF Jr

更新日期：1985-05-01 00:00:00

abstract：:Suggestions are made for the estimation of plasmid loss rates in bacterial populations that multiply by binary fission. The plasmid loss rate is defined as the probability of a division of a plasmid-carrying individual giving birth to one plasmid-free and one plasmid-carrying daughter cell. The unit of time is consequ...

journal_title：Plasmid

authors： Boe L,Rasmussen KV

更新日期：1996-11-01 00:00:00

abstract：:We report here the molecular characterization of pFNL10, a 3990-bp cryptic plasmid of Francisella novicida-like F6168. The plasmid was maintained in F. novicida Utah 112 and F. tularensis LVS strains. We sequenced the entire plasmid and found six open reading frames (ORFs)-ORF1, ORF2, ORF3, ORF4, ORF5, and ORFm. ORF3,...

journal_title：Plasmid

authors： Pomerantsev AP,Golovliov IR,Ohara Y,Mokrievich AN,Obuchi M,Norqvist A,Kuoppa K,Pavlov VM

更新日期：2001-11-01 00:00:00

abstract：:Mutations that increase the copy number of the pSC101 replicon have been used for construction of new cloning vectors. Replacement of glutamate at position 93 in RepA yields plasmids that replicate at medium (27 copies/cell) and high (approximately 240 copies/cell) copy numbers. Based on the crystal structure of RepE,...

journal_title：Plasmid

authors： Peterson J,Phillips GJ

更新日期：2008-05-01 00:00:00

abstract：:Sphingomonas sp. A1 (strain A1) is capable of directly incorporating macromolecules (e.g., alginate) through the specialized import system--"super-channel." Here, we report the complete DNA sequence and genetic organization of plasmid pA1 from strain A1. Nucleotide sequence analysis revealed that pA1 comprises 46,557 ...

journal_title：Plasmid

authors： Harada KM,Aso Y,Hashimoto W,Mikami B,Murata K

更新日期：2006-07-01 00:00:00

abstract：:Despite the prevalence of Arthrobacter in the environment little is known about their plasmids, or the capacity of Arthrobacter plasmids to mediate horizontal gene transfer. In this study, we compared eight plasmids from five Arthrobacter strains in order to identify putative core maintenance genes for replication, se...

journal_title：Plasmid

authors： Jerke K,Nakatsu CH,Beasley F,Konopka A

更新日期：2008-03-01 00:00:00

abstract：:Unequal crossing-over is involved in genetic duplication and deletion in such diverse genetic systems as Drosophila, bacteria, and animal viruses. It is proposed to be involved in the form of unequal sister chromatid exchange in gene amplification in cultured animal cells and during carcinogenesis. Studies of the proc...

journal_title：Plasmid

authors： Clevenger W,Reeves R,Pall ML

更新日期：1985-05-01 00:00:00

abstract：:pSAM2 is an 11-kb plasmid integrated in the Streptomyces ambofaciens ATCC23877 and ATCC15154 genomes and found additionally as a free replicon in an uv derivative. After transfer into S. ambofaciens DSM40697 (devoid of pSAM2) or into Streptomyces lividans, specific integration of pSAM2 occurred very efficiently. A 58-...

journal_title：Plasmid

authors： Boccard F,Smokvina T,Pernodet JL,Friedmann A,Guérineau M

更新日期：1989-01-01 00:00:00

abstract：:Acinetobacter baumannii is an important nosocomial pathogen that often complicates treatment because of its high level of resistance to antibiotics. Though plasmids can potentially introduce various genes into bacterial strains, compared to other Gram-negative bacteria, information about the unique A. baumannii plasmi...

journal_title：Plasmid

authors： Hamidian M,Ambrose SJ,Hall RM

更新日期：2016-01-01 00:00:00

abstract：:A simple and reliable method for the determination of plasmid copy numbers by direct fluorescence densitometry of ethidium bromide-stained electrophoretic gels was developed. In developing the method, the following parameters were evaluated and controlled: plasmid DNA trapping in the linear chromosomal DNA, staining-d...

journal_title：Plasmid

authors： Projan SJ,Carleton S,Novick RP

更新日期：1983-03-01 00:00:00

abstract：:Glycopeptide resistant Enterococcus faecium (GREF) persists on Norwegian poultry farms despite the ban on the growth promoter avoparcin. The biological basis for long-term persistence of avoparcin resistance is not fully understood. This study presents the complete DNA sequence of the E. faecium R-plasmid pVEF3 and fu...

journal_title：Plasmid

authors： Sletvold H,Johnsen PJ,Hamre I,Simonsen GS,Sundsfjord A,Nielsen KM

更新日期：2008-07-01 00:00:00

abstract：:pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...

journal_title：Plasmid

authors： Chiang CS,Bremer H

更新日期：1991-11-01 00:00:00

abstract：:Since reduced metronidazole causes DNA damage, resistance to metronidazole was used as a selection method for the cloning of Bacteroides fragilis genes affecting DNA repair mechanisms in Escherichia coli. Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli AB1157 and uvrA, B, and...

journal_title：Plasmid

authors： Wehnert GU,Abratt VR,Goodman HJ,Woods DR

更新日期：1990-03-01 00:00:00

abstract：:The plasmid pMSP3535 is a popular vector for nisin-inducible expression of heterologous genes in lactic acid bacteria. However, the maximum protein expression level achievable with pMSP3535 is relatively low. In an effort to increase expression we modified pMSP3535 to create a high expression variant termed pMSP3535H2...

journal_title：Plasmid

authors： Kim JH,Mills DA

更新日期：2007-11-01 00:00:00

abstract：:We report the characterization of Tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the R-plasmid pGD10. Tn602 is highly homologous to the previously characterized Tn903, present on the R-plasmid R6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-I (hom...

journal_title：Plasmid

authors： Stibitz S,Davies JE

更新日期：1987-05-01 00:00:00