Development of shuttle vectors for evaluation of essential regulator regulated gene expression in Staphylococcus aureus.

abstract：:The region responsible for the maintenance of the prophage of bacteriophage P7 as a stable, unit-copy plasmid was isolated in a lambda att vector which lysogenizes Escherichia coli as a stable unit-copy plasmid under the control of the P7 replication origin. The P7 plasmid-maintenance region was shown to consist of ad...

journal_title：Plasmid

authors： Ludtke DN,Austin SJ

更新日期：1987-07-01 00:00:00

abstract：:Homology was previously detected between the DNA restriction fragments containing Rhizobium meliloti nodulation genes and the 90-MDa plasmid, p90, of Azospirillum brasilense Sp7. Two DNA loci from Sp7 genome that complement mutations in the exopolysaccharide synthesis genes, exoB and exoC, of R. meliloti were also sho...

journal_title：Plasmid

authors： Onyeocha I,Vieille C,Zimmer W,Baca BE,Flores M,Palacios R,Elmerich C

更新日期：1990-05-01 00:00:00

abstract：:The large, 350-kb Sym (symbiotic) plasmid pRjaUSDA193 of Rhizobium fredii was examined to determine the frequency of repeated sequences present and to produce a physical and genetic map of a large region of the plasmid. A novel hybridization method, the Southern Cross, revealed that the plasmid pRjaUSDA193 contained m...

journal_title：Plasmid

authors： Masterson RV,Atherly AG

更新日期：1986-07-01 00:00:00

abstract：:pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper s...

journal_title：Plasmid

authors： Leclercq J,Szabolcs T,Martin F,Montoro P

更新日期：2015-09-01 00:00:00

abstract：:Expression vectors for industrial production should be stable and allow tight control of protein synthesis. This is necessary to ensure plasmid transmission to daughter cells in order to achieve a stable population capable of synthesizing high amounts of the target protein. A high-copy-number plasmid, pAE, was previou...

journal_title：Plasmid

authors： Eguia FAP,Ramos HR,Kraschowetz S,Omote D,Ramos CRR,Ho PL,Carvalho E,Gonçalves VM

更新日期：2018-06-01 00:00:00

abstract：:Two B/O plasmids were sequenced. The kanamycin resistance plasmid R805a was found in a Salmonella Typhi strain from a 1972 typhoid fever outbreak in Mexico City. pCERC6, which confers resistance to ampicillin, streptomycin and sulphamethoxazole, was found in a commensal E. coli isolated from a healthy adult in Sydney ...

journal_title：Plasmid

authors： Moran RA,Richardson IA,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:Nucleotide sequence analysis of two cryptic plasmids, pRS2 (2544 bp) and pRS3 (3948 bp), from Oenococcus oeni revealed the presence in both of three major open reading frames with significant similarity to other small cryptic plasmids from O. oeni. The results suggest that those plasmids could be separated into two su...

journal_title：Plasmid

authors： Mesas JM,Rodríguez MC,Alegre MT

更新日期：2001-09-01 00:00:00

abstract：:Despite the prevalence of Arthrobacter in the environment little is known about their plasmids, or the capacity of Arthrobacter plasmids to mediate horizontal gene transfer. In this study, we compared eight plasmids from five Arthrobacter strains in order to identify putative core maintenance genes for replication, se...

journal_title：Plasmid

authors： Jerke K,Nakatsu CH,Beasley F,Konopka A

更新日期：2008-03-01 00:00:00

abstract：:A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...

journal_title：Plasmid

authors： David M,Lubinsky-Mink S,Ben-Zvi A,Ulitzur S,Kuhn J,Suissa M

更新日期：1992-11-01 00:00:00

abstract：:Unequal crossing-over is involved in genetic duplication and deletion in such diverse genetic systems as Drosophila, bacteria, and animal viruses. It is proposed to be involved in the form of unequal sister chromatid exchange in gene amplification in cultured animal cells and during carcinogenesis. Studies of the proc...

journal_title：Plasmid

authors： Clevenger W,Reeves R,Pall ML

更新日期：1985-05-01 00:00:00

abstract：:A flexible M13 vector library incorporating the BstXI site has been developed. DNA cut by any currently commercially available restriction endonuclease that generates a 4-nucleotide (nt) 3' overhang can be ligated into a specific clone of the library. The BstXI enzyme recognizes a 6-bp bipartite palindromic sequence. ...

journal_title：Plasmid

authors： Panchmatia FK,Cramb EM,Li V,Allen FL,Glickman BW,Waye MM

更新日期：1990-07-01 00:00:00

abstract：:Naturally occurring plasmids have medical importance given that they frequently code for virulence or antibiotic resistance. In many cases, plasmids impose a fitness cost to their hosts, meaning that the growth rate of plasmid-bearing cells is lower than that of plasmid-free cells. However, this does not fit with the ...

journal_title：Plasmid

authors： Gama JA,Zilhão R,Dionisio F

更新日期：2018-09-01 00:00:00

abstract：:A novel type I restriction-modification specificity subunit, S. LlaW12I, has been identified on the naturally occurring 8.0-kb plasmid pAW122 in the lactic acid bacterium Lactococcus lactis subsp. cremoris W12. Presence of the HsdS protein together with a complete type I restriction-modification system conferred incre...

journal_title：Plasmid

authors： Madsen A,Westphal C,Josephsen J

更新日期：2000-09-01 00:00:00

abstract：:Suggestions are made for the estimation of plasmid loss rates in bacterial populations that multiply by binary fission. The plasmid loss rate is defined as the probability of a division of a plasmid-carrying individual giving birth to one plasmid-free and one plasmid-carrying daughter cell. The unit of time is consequ...

journal_title：Plasmid

authors： Boe L,Rasmussen KV

更新日期：1996-11-01 00:00:00

abstract：:The complete sequence of the 10.9-kbp bacteriocinogenic plasmid pBL1 from Lactococcus lactis subsp. lactis IPLA 972 has been determined. Thirteen ORFs were encountered, of which 5 were incomplete. pBL1 proved to be a narrow-host-range plasmid which replicates neither in Bacilus subtilis nor in Lactobacillus spp. The s...

journal_title：Plasmid

authors： Sánchez C,Hernández de Rojas A,Martínez B,Argüelles ME,Suárez JE,Rodríguez A,Mayo B

更新日期：2000-11-01 00:00:00

abstract：:We have examined the extent to which the degradative plasmids SAL, NAH, and TOL of the Inc P-9 incompatibility group share common DNA sequences. The homology we observe using 32P-labeled SAL and NAH DNA probes can be assigned to six regions of the TOL (pWWO) restriction endonuclease cleavage map. At least three of the...

journal_title：Plasmid

authors： Lehrbach PR,McGregor I,Ward JM,Broda P

更新日期：1983-09-01 00:00:00

abstract：:The linear plasmid SCP1 of Streptomyces coelicolor A3(2) is one of the genetically more studied linear streptomycete replicons. Although the genetics of SCP1 and its interaction with the host chromosome have been analyzed for nearly three decades no information exists on its replication. With the help of an ordered co...

journal_title：Plasmid

authors： Redenbach M,Bibb M,Gust B,Seitz B,Spychaj A

更新日期：1999-11-01 00:00:00

abstract：:Bacillus mycoides, a member of the Bacillus cereus group of bacteria, can be easily distinguished from close species because of colony shape, made by filaments of cells, resembling fungal hyphae, curving clock- or counterclockwise depending on the strain. Two plasmids, one from a strain curving to the right (pDx14.2),...

journal_title：Plasmid

authors： Di Franco C,Santini T,Pisaneschi G,Beccari E

更新日期：2005-11-01 00:00:00

abstract：:A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimat...

journal_title：Plasmid

authors： Coronado C,Vázquez ME,Cebolla A,Palomares AJ

更新日期：1994-11-01 00:00:00

abstract：:The imidazole and triazole fungicides inhibit cytochrome P450 14 alpha-lanosterol demethylase (P45014DM) implicated in the ergosterol biosynthesis pathway, which is specific to fungi and yeasts. Two plasmids were obtained which allow triazole and imidazole resistance in Saccharomyces cerevisiae. The low copy number pl...

journal_title：Plasmid

authors： Doignon F,Aigle M,Ribereau-Gayon P

更新日期：1993-11-01 00:00:00

abstract：:The complete nucleotide sequence of the cryptic plasmid pCC1 from Corynebacterium callunae (4109 bp) was determined. DNA sequence analysis revealed five open reading frames longer than 200 bp. One of the deduced polypeptides showed homology with the Rep proteins encoded by plasmids of the pIJ101/pJV1 family of plasmid...

journal_title：Plasmid

authors： Venkova-Canova T,Pátek M,Nesvera J

更新日期：2004-01-01 00:00:00

abstract：:Ten strains of Rhodopseudomonas sphaeroides were analyzed by agarose gel electrophoresis for plasmid DNA content and, by filter-hybridizations, for their molecular relationships. All strains examined contained at least one plasmid. Several strains carried as many as six different plasmid species with sizes ranging fro...

journal_title：Plasmid

authors： Fornari CS,Watkins M,Kaplan S

更新日期：1984-01-01 00:00:00

abstract：:In the present study we report the complete nucleotide sequence of the toluene catabolic plasmid pD2RT of Pseudomonas migulae strain D2RT isolated from Baltic Sea water. The pD2RT is 129,894 base pairs in size with an average G+C content of 53.75%. A total of 135 open reading frames (ORFs) were predicted to encode pro...

journal_title：Plasmid

authors： Jutkina J,Hansen LH,Li L,Heinaru E,Vedler E,Jõesaar M,Heinaru A

更新日期：2013-11-01 00:00:00

abstract：:A 102-kb plasmid, pIJB1, was isolated from Burkholderia cepacia strain 2a, which is able to use 2,4-dichlorophenoxyacetate (2,4-D) as a sole carbon source, and a physical map of the plasmid has been established. It was observed that spontaneous loss of a 37-kb fragment of the plasmid after growth in nonselective mediu...

journal_title：Plasmid

authors： Xia XS,Aathithan S,Oswiecimska K,Smith AR,Bruce IJ

更新日期：1998-01-01 00:00:00

abstract：:Some strains of the Enterobacteriaceae Serratia entomophila and Serratia proteamaculans cause amber disease in the grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. The genes responsible for this disease reside on a large, 155-kb plasmid designated amber disease-as...

journal_title：Plasmid

authors： Hurst MR,O'Callaghan M,Glare TR

更新日期：2003-11-01 00:00:00

abstract：:Aeromonas hydrophila strain AO1 isolated from an infected fish was found to be resistant to several quinolones. A plasmid isolated from the strain AO1, termed pBRST7.6, was cloned and sequenced and shown to be 7621bp in length with a GC content of 60%. Further analysis confirmed that it contained a gene with 100% iden...

journal_title：Plasmid

authors： Majumdar T,Das B,Bhadra RK,Dam B,Mazumder S

更新日期：2011-07-01 00:00:00

abstract：:Escherichia coli pcnB gene was discovered as a regulator of ColE1 plasmid copy number about 20 years ago. Further studies indicated that this gene codes for poly(A) polymerase I (PAP I). However, although importance of RNA polyadenylation has been demonstrated and biochemical properties of PAP I have been studied exte...

journal_title：Plasmid

authors： Jasiecki J,Wegrzyn G

更新日期：2006-03-01 00:00:00

abstract：:As part of a study identifying plasmids in Campylobacter, we isolated and sequenced two novel cryptic plasmids from an agricultural isolate of Campylobacter coli. The larger of the two plasmids, p3384, is 3316 bp in length and has a G+C content of 31.18%. A typical origin of replication consisting of five iterons was ...

journal_title：Plasmid

authors： Jesse TW,Pittenger-Alley LG,Englen MD

更新日期：2006-01-01 00:00:00

abstract：:Two independently isolated temperature-sensitive bacteriophage that are specific for enterobacterial hosts harboring HI and HII plasmids were characterized to determine if any identifiable differences existed between them. The traits examined included adsorption pattern of phage to H pili, bacteriophage size, sensitiv...

journal_title：Plasmid

authors： Maher D,Sherburne R,Taylor DE

更新日期：1991-09-01 00:00:00

abstract：:Horizontal transfer of genes encoding phenol degradation (pheBA) in the environment has been previously described. Complete or partial phe-operon was redetected in plasmids of several indigenous Pseudomonas strains isolated from the river water. The sequences of up- and downstream regions of the acquired phe-DNA in ei...

journal_title：Plasmid

authors： Peters M,Tomikas A,Nurk A

更新日期：2004-11-01 00:00:00