Abstract：
:pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper sets out to convert the pCambia2300 binary vector into a destination vector with the Gateway® cassette driven by the CaMV35S promoter. The destination vector, pCamway35S, was then evaluated using the uidA reporter gene. Transient and stable transformation experiments were successfully assayed, either by particle bombardment or by Agrobacterium tumefaciens in Allium cepa and Hevea embryogenic calli. After counting the transformation units, the statistical analysis performed on the data showed that the pCamway 35S::uidA vector was as efficient as pCambia2301, a pCAMBIA2300 containing the uidA reporter gene under the CaMV 35S promoter.
journal_name
Plasmidjournal_title
Plasmidauthors
Leclercq J,Szabolcs T,Martin F,Montoro Pdoi
10.1016/j.plasmid.2015.07.003subject
Has Abstractpub_date
2015-09-01 00:00:00pages
50-4eissn
0147-619Xissn
1095-9890pii
S0147-619X(15)30006-8journal_volume
81pub_type
杂志文章相关文献
PLASMID文献大全abstract：:Plasmid pT181 is a small multicopy plasmid from Staphylococcus aureus that belongs to incompatibility group 3 and expresses two distinct types of incompatibility, Inc3A and Inc3B. Inc3A incompatibility is expressed by the primary replication control determinant, copA, which specifies two small transcripts, RNA I and R...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(90)90039-f
更新日期：1990-01-01 00:00:00
abstract：:The cloning of the finO region from the antibiotic-resistance plasmid R6-5 is reported. On the basis of DNA deletion analysis and Tn5 transposon insertional mutagenesis of finO+ chimeric plasmids, finO has been located within the coordinates 94.0-94.85 on the R6-5 map. A 32,000-Da polypeptide (32K), which is encoded w...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(84)90050-7
更新日期：1984-11-01 00:00:00
abstract：:Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons. Amplification products were obtained from 20 of the hybridization-positive strains. A single dfrA7 cassette was identified in 18 of the isolates and an integron with two cassettes (aadB-aadA4) ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/s0147-619x(03)00011-8
更新日期：2003-03-01 00:00:00
abstract：:pSM19035 is a low-copy-number theta-replicating plasmid, which belongs to the Inc18 family. Plasmids of this family, which show a modular organization, are functional in evolutionarily diverse bacterial species of the Firmicutes Phylum. This review summarizes our understanding, accumulated during the last 20 years, on...
journal_title：Plasmid
pub_type： 杂志文章,评审
doi：10.1016/j.plasmid.2010.04.002
更新日期：2010-07-01 00:00:00
abstract：:We report the mobilization by cointegration of the gonococcal 5.2 kb beta-lactamase plasmid pSJ5.2 in an Escherichia coli background. Transfer of pSJ5.2 was measured by filter mating assays with five different conjugative plasmids from Enterobacteriaceae and the gonococcal 41 kb tet(M). Plasmid pSJ5.2 was mobilized to...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2006.07.006
更新日期：2007-03-01 00:00:00
abstract：:Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1997.1289
更新日期：1997-01-01 00:00:00
abstract：:A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(92)90059-j
更新日期：1992-11-01 00:00:00
abstract：:We report the characterization of Tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the R-plasmid pGD10. Tn602 is highly homologous to the previously characterized Tn903, present on the R-plasmid R6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-I (hom...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(87)90028-x
更新日期：1987-05-01 00:00:00
abstract：:We have cloned and characterized an insertion sequence from Leuconostoc mesenteroides subsp. cremoris strain DB1165. This element, designated IS1165, is 1553 bp, has imperfect inverted repeat ends, contains an open reading frame of 1236 bp, and is not related to any previously described insertion sequence. The copy nu...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(92)90022-3
更新日期：1992-05-01 00:00:00
abstract：:A preliminary screening study of six Moraxella catarrhalis isolates from primary school children in the Netherlands identified a small 3.5 kb plasmid (pEMCJH03), containing four open reading frames, which encoded three mobilizing and one replicase protein. Insertion of a kanamycin containing transposon (yielding pEMCJ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2004.11.003
更新日期：2005-05-01 00:00:00
abstract：:The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of co...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(87)90009-6
更新日期：1987-01-01 00:00:00
abstract：:Naturally occurring plasmids have medical importance given that they frequently code for virulence or antibiotic resistance. In many cases, plasmids impose a fitness cost to their hosts, meaning that the growth rate of plasmid-bearing cells is lower than that of plasmid-free cells. However, this does not fit with the ...
journal_title：Plasmid
pub_type： 杂志文章,评审
doi：10.1016/j.plasmid.2018.09.009
更新日期：2018-09-01 00:00:00
abstract：:Tn4527 was isolated from a Salmonella typhimurium strain obtained in Brazil. Its size is 19.6 kb and it carries resistance to trimethoprim, spectinomycin, and streptomycin, as in the case of Tn7 (14 kb). A restriction analysis of the transposon shows regions of similarity to Tn7 mixed with extra DNA. The 2.6-kb and 2....
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(89)90009-7
更新日期：1989-11-01 00:00:00
abstract：:The incompatibility region of the Streptomyces plasmid pSL1 was identified as a 240-bp segment, though some other function from the vector plasmid was also necessary. A 540-bp fragment including the 240-bp region was enough for full expression of incompatibility. Inserted mutation analysis led to a more detailed locat...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(87)90021-7
更新日期：1987-03-01 00:00:00
abstract：:The Gram-positive Corynebacterium glutamicum is widely known for its application in the industrial production of amino acids and as a non-pathogenic model organism for cell wall biosynthesis in the group of CMN bacteria. For biotechnological and physiological studies often co-expression of recombinant genes is require...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2018.01.001
更新日期：2018-01-01 00:00:00
abstract：:The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/s0147-619x(03)00059-3
更新日期：2003-09-01 00:00:00
abstract：:pIP501 and pAM beta 1 are promiscuous plasmids from gram-positive bacteria which carry structurally and genetically related replication functions (L. Jannière, A. Gruss and S.D. Ehrlich, 1993, in "Bacillus subtilis and Other Gram-positive Bacteria: Biochemistry, Physiology, and Molecular Genetics (A.L. Sonenshein, J.A...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1994.1010
更新日期：1994-01-01 00:00:00
abstract：:Temperate bacteriophage MAV1 is found in certain highly virulent strains of Mycoplasma arthritidis. Integration sites, portions of the right and left prophage ends, and flanking DNA from eight prophages in seven M. arthritidis strains were characterized in this study. attb and attp sites conformed for the most part to...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2004.04.004
更新日期：2004-07-01 00:00:00
abstract：:pBTK445 is a newly described large (∼60Kb), low-copy number, conjugative plasmid indigenous to the sulfur-chemolithoautotroph Advenella kashmirensis. Based on its minimal replication region, a shuttle vector, pBTKS was constructed which can be used for diverse Alcaligenaceae members. The construct was found to be stab...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2010.12.007
更新日期：2011-03-01 00:00:00
abstract：:The 4.2-kb cryptic plasmid pVA380-1 has been used as a vector for the cloning of antibiotic resistance genes directly in streptococci, and in the construction of Escherichia coli/Streptococcus shuttle vectors. The results of subcloning experiments located the basic replicon of pVA380-1 within a 2.5-kb region. The nucl...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(92)90044-b
更新日期：1992-09-01 00:00:00
abstract：:Nonviral gene delivery has gained a lot of interest as a promising approach for gene therapy. Despite intensive studies and much progress the outcome of nonviral vectors has remained significantly weaker than that of viral vectors. A weak transfection efficiency of nonviral gene transfection is still limiting their in...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2009.09.003
更新日期：2010-01-01 00:00:00
abstract：:Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vec...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/s0147-619x(03)00030-1
更新日期：2003-07-01 00:00:00
abstract：:The complete nucleotide sequence of the cryptic plasmid pCC1 from Corynebacterium callunae (4109 bp) was determined. DNA sequence analysis revealed five open reading frames longer than 200 bp. One of the deduced polypeptides showed homology with the Rep proteins encoded by plasmids of the pIJ101/pJV1 family of plasmid...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2003.09.002
更新日期：2004-01-01 00:00:00
abstract：:A small cryptic plasmid designated pHW15 was isolated from Rahnella genomospecies 2 WMR15 and its complete nucleotide sequence was determined. The plasmid contained 3002 bp with a G+C content of 47.4%. The origin of replication was identified by deletion analysis as a region of about 600 bp. This region had an identit...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2006.05.007
更新日期：2006-11-01 00:00:00
abstract：:To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2008.09.006
更新日期：2009-01-01 00:00:00
abstract：:Nocardia brasiliensis, is a bacteria that lives as saprophyte in soil and causes a disease called actinomycetoma in both human and animals. Nocardia brasiliensis is an intracellular, facultative bacterium that replicates and survives within host macrophages. The mechanisms involved in the evasion of the microbicidal a...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2010.09.005
更新日期：2011-01-01 00:00:00
abstract：:We describe the construction of a series of shuttle vectors for Staphylococcus aureus. In order to determine transcriptional regulation by essential regulators, we constructed promoterless luxABCDE reporter system using a TetR-regulated antisense RNA expression vector, pJYJ909, which is composed of S. aureus plasmid p...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2009.02.001
更新日期：2009-05-01 00:00:00
abstract：:The conjugative transfer system of Yersinia enterocolitica 29930 present on the cryptic plasmid p29930 comprises a mating pore formation system (Mpf) related to that of the IncX plasmid R6K and a DNA transfer and replication system (Dtr) with close relationship to the mob region of the mobilizable plasmid CloDF13. Two...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2010.05.001
更新日期：2010-09-01 00:00:00
abstract：:The structural and functional properties of mercury resistance determinants cloned from a series of independently isolated conjugative plasmids were compared with those of the prototype HgR determinants from Tn501 and plasmid R100 (containing Tn21). Restriction endonuclease mapping classified the HgR determinants into...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(88)90013-3
更新日期：1988-09-01 00:00:00
abstract：:Avirulence gene D (avrD) in strain PT23 of Pseudomonas syringae pv. tomato (Pst) specifies the production of syringolides, which are elicitors of plant defense reactions. An 83-kb indigenous plasmid (pPT23B) that carries avrD has been mapped and characterized and a putative par region was identified. pPT23B contains a...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1994.1029
更新日期：1994-05-01 00:00:00
