Abstract:
:Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons. Amplification products were obtained from 20 of the hybridization-positive strains. A single dfrA7 cassette was identified in 18 of the isolates and an integron with two cassettes (aadB-aadA4) was found in only one strain. Amplicons were not obtained from one of the hybridization positive strains. DNA sequence analysis of a 6.080-kb fragment, cloned from this strain, identified the remnant of an integron, following insertion of IS26 into the 5(')-end of intI1. The 6.080-kb sequence carries an aminoglycoside resistance gene, linked to a portion of IS1133, which in turn is linked to a sequence that has properties of IS elements, including sequences that could stimulate transcription, and ORFs encoding amino acid sequences with similarity to a transposase from Deinococcus radiodurans.
journal_name
Plasmidjournal_title
Plasmidauthors
Segal H,Thomas R,Gay Elisha Bdoi
10.1016/s0147-619x(03)00011-8keywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
169-78issue
2eissn
0147-619Xissn
1095-9890pii
S0147619X03000118journal_volume
49pub_type
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