Abstract:
:In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restriction and hybridization experiments revealed that identical ISp elements could be isolated from different R. meliloti strains and that one of these is similar to an insertion sequence found previously in R. meliloti 2011.
journal_name
Plasmidjournal_title
Plasmidauthors
Labes G,Simon Rdoi
10.1016/0147-619x(90)90007-ysubject
Has Abstractpub_date
1990-11-01 00:00:00pages
235-9issue
3eissn
0147-619Xissn
1095-9890journal_volume
24pub_type
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