当前位置： SCI文献检索 > PLASMID期刊下所有文献 > Cloning, sequencing, and sequence analysis of two novel plasmids from the thermophilic anaerobic bacterium Anaerocellum thermophilum.

Cloning, sequencing, and sequence analysis of two novel plasmids from the thermophilic anaerobic bacterium Anaerocellum thermophilum.

Abstract：

:The nucleotide sequence of two novel plasmids isolated from the extreme thermophilic anaerobic bacterium Anaerocellum thermophilum DSM6725 (A. thermophilum), growing optimally at 70 degrees C, has been determined. pBAS2 was found to be a 3653 bp plasmid with a GC content of 43%, and the sequence revealed 10 open reading frames (ORFs). The two largest of these, namely Orf21 and Orf41, showed similarity to a Bacillus plasmid recombinase and a Pseudoalteromonas plasmid replication protein, respectively. A sequence with homology to double stranded replication origins from rolling circle plasmids was found, but no single stranded intermediates, characteristic of rolling circle replication, were found on Southern blots. The larger plasmid, pBAL, was found to be a 8294 bp plasmid with a GC content of 39%. It revealed 17 ORFs, of which three showed similarity at the amino acid (aa) level to known proteins. Orf22 showed the strongest similarity (33% aa) to replication proteins from large multiresistance Staphylococcal and Lactococcal plasmids, all of which are believed to replicate via a theta-like replication mechanism. Orf32 showed similarity to both DNA repair proteins and DNA polymerases with highest similarity to DNA repair protein from Campylobacter jejuni (25% aa). Orf34 showed similarity to sigma factors with highest similarity (28% aa) to the sporulation specific Sigma factor, Sigma 28(K) from Bacillus thuringiensis.

journal_name

Plasmid

journal_title

Plasmid

authors

Clausen A,Mikkelsen MJ,Schröder I,Ahring BK

doi

10.1016/j.plasmid.2004.06.001

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

131-8

issue

2

eissn

0147-619X

issn

1095-9890

pii

S0147619X04000770

journal_volume

52

pub_type

杂志文章

相关文献

PLASMID文献大全
  • Mutational and physiological analyses of plasmid pT181 functions expressing incompatibility.

    abstract：:Plasmid pT181 is a small multicopy plasmid from Staphylococcus aureus that belongs to incompatibility group 3 and expresses two distinct types of incompatibility, Inc3A and Inc3B. Inc3A incompatibility is expressed by the primary replication control determinant, copA, which specifies two small transcripts, RNA I and R...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(90)90039-f

    authors： Highlander SK,Novick RP

    更新日期：1990-01-01 00:00:00

  • High-level expression vectors for Caulobacter crescentus incorporating the transcription/translation initiation regions of the paracrystalline surface-layer-protein gene.

    abstract：:A number of plasmid vectors were constructed for high-level gene expression in the dimorphic gram-negative bacterium Caulobacter crescentus. These vectors incorporate the transcription and translation initiation regions of the C. crescentus CB15A rsaA gene, which codes for the abundantly synthesized protein comprising...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(90)90016-6

    authors： Bingle WH,Smit J

    更新日期：1990-09-01 00:00:00

  • Dosage of Tn916 circular intermediates in Enterococcus faecalis.

    abstract：:Upon excision, conjugative transposon Tn916 forms a nonreplicating circular intermediate (CI), which is essential both for transposition and conjugal transfer. In this work we developed an assay to quantify the circular forms of Tn916. By this method we defined a new measurable trait of Tn916-carrying strains: the "CI...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1995.1032

    authors： Manganelli R,Romano L,Ricci S,Zazzi M,Pozzi G

    更新日期：1995-07-01 00:00:00

  • The complete nucleotide sequence of a small cryptic plasmid from a rumen bacterium of the genus Butyrivibrio.

    abstract：:The complete nucleotide sequence of a plasmid, designated pRJF1, isolated from a rumen bacterium of the genus Butyrivibrio, has been determined. pRJF1 is a small plasmid (2631 bp) which shows the high AT content (64%) typical of plasmids from gram-positive organisms. Computer analysis of sequence data revealed two maj...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1993.1008

    authors： Hefford MA,Teather RM,Forster RJ

    更新日期：1993-01-01 00:00:00

  • The promiscuous plasmids pIP501 and pAM beta 1 from gram-positive bacteria encode complementary resolution functions.

    abstract：:pIP501 and pAM beta 1 are promiscuous plasmids from gram-positive bacteria which carry structurally and genetically related replication functions (L. Jannière, A. Gruss and S.D. Ehrlich, 1993, in "Bacillus subtilis and Other Gram-positive Bacteria: Biochemistry, Physiology, and Molecular Genetics (A.L. Sonenshein, J.A...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1994.1010

    authors： Pujol C,Ehrlich SD,Jannière L

    更新日期：1994-01-01 00:00:00

  • Search for a novel killer toxin in yeast Pichia pastoris.

    abstract：:Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have b...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1999.1452

    authors： Banerjee H,Verma M

    更新日期：2000-03-01 00:00:00

  • Peaceful coexistence amongst Borrelia plasmids: getting by with a little help from their friends?

    abstract：:Borrelia species comprise a unique genus of bacterial pathogens. These organisms contain a segmented genome with up to two dozen plasmids ranging in size from 5 kb up to about 200 kb. The plasmids have also been referred to as mini-chromosomes or essential genetic elements, as some of them carry information important ...

    journal_title：Plasmid

    pub_type： 杂志文章,评审

    doi：10.1016/j.plasmid.2013.05.002

    authors： Chaconas G,Norris SJ

    更新日期：2013-09-01 00:00:00

  • A novel plasmid (pEMCJH03) isolated from moraxella catarrhalis possibly useful as a cloning and expression vector within this species.

    abstract：:A preliminary screening study of six Moraxella catarrhalis isolates from primary school children in the Netherlands identified a small 3.5 kb plasmid (pEMCJH03), containing four open reading frames, which encoded three mobilizing and one replicase protein. Insertion of a kanamycin containing transposon (yielding pEMCJ...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2004.11.003

    authors： Hays JP,Eadie K,Verduin CM,Verbrugh H,van Belkum A

    更新日期：2005-05-01 00:00:00

  • Complete nucleotide sequence of the self-transmissible TOL plasmid pD2RT provides new insight into arrangement of toluene catabolic plasmids.

    abstract：:In the present study we report the complete nucleotide sequence of the toluene catabolic plasmid pD2RT of Pseudomonas migulae strain D2RT isolated from Baltic Sea water. The pD2RT is 129,894 base pairs in size with an average G+C content of 53.75%. A total of 135 open reading frames (ORFs) were predicted to encode pro...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2013.09.003

    authors： Jutkina J,Hansen LH,Li L,Heinaru E,Vedler E,Jõesaar M,Heinaru A

    更新日期：2013-11-01 00:00:00

  • Inclusion of a matrix-attached region in a 7SK pol III vector increases the efficiency of shRNA-mediated gene silencing in embryonic carcinoma cells.

    abstract：:RNA interference is a widely used tool for analysis of gene function in mammalian cells. Stable knockdown of specific target genes can be maintained in cell lines and live organisms using vector-based delivery of short hairpins (shRNAs) driven by RNA polymerase III promoters. Here we describe a vector incorporating th...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2005.11.008

    authors： Sotirova VN,Calciano MA,Krueger W,Lalande M

    更新日期：2006-05-01 00:00:00

  • Comparative analysis of staphylococcal plasmids carrying three streptogramin-resistance genes: vat-vgb-vga.

    abstract：:Several staphylococcal plasmids (26-45 kb) carry all three streptogramin-resistance (Sg(R)) genes, vat, vgb, and vga. Seven such plasmids harbored by independent strains belonging to three taxa (Staphylococcus aureus, S. simulans, and S. cohnii subsp. urealyticum) were compared and the deleted derivative of one of the...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1999.1412

    authors： Allignet J,El Solh N

    更新日期：1999-09-01 00:00:00

  • Polymorphism of Nopaline-type T-DNAs from Agrobacterium tumefaciens.

    abstract：:The structure of several T-DNAs of Agrobacterium tumefaciens was determined by molecular cloning and Southern hybridization. The T-DNAs cloned in Escherichia coli vectors from four different nopaline type strains (PyTE1, PO31, PO22, and AKE10) showed various sizes of restriction enzyme fragments. Comparative analysis ...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(91)90002-e

    authors： Wabiko H,Kagaya M,Sano H

    更新日期：1991-01-01 00:00:00

  • Interaction between mitochondrial genes in yeast: evidence for novel box effect(s).

    abstract：:The effects of mutations have been studied within the apocytochrome b gene on the processing of transcripts from the gene for subunit 1 of cytochrome c oxidase (coxI). Most mutations which affect the expression of the reading frame encoded by the fourth intron of the apocytochrome b gene (bI4) result in a failure to r...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(84)90065-9

    authors： Hensgens LA,Van der Horst G,Grivell LA

    更新日期：1984-07-01 00:00:00

  • Compatible Dictyostelium mucoroides nuclear plasmids Dmp1 and Dmp2 both belong to the Ddp1 plasmid family.

    abstract：:Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurring compatible members of the Dictyostelium Ddp1 plasmid family found in the same wild isolate strain. The nucleotide sequences of Dmp1 (5983 bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading frames (ORFs) similar to the G1 and G5...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1994.1013

    authors： Kiyosawa H,Hughes JE,Welker DL

    更新日期：1994-03-01 00:00:00

  • A large conjugative Acinetobacter baumannii plasmid carrying the sul2 sulphonamide and strAB streptomycin resistance genes.

    abstract：:Acinetobacter baumannii is an important nosocomial pathogen that often complicates treatment because of its high level of resistance to antibiotics. Though plasmids can potentially introduce various genes into bacterial strains, compared to other Gram-negative bacteria, information about the unique A. baumannii plasmi...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2016.09.001

    authors： Hamidian M,Ambrose SJ,Hall RM

    更新日期：2016-01-01 00:00:00

  • A Brevibacterium linens pRBL1 replicon functional in Corynebacterium glutamicum.

    abstract：:Brevibacterium linens RBL strain cryptic plasmid pRBL1 (8.0 kb) is described. A region involved in pRBL1 autonomous replication in Corynebacterium glutamicum was identified by insertion and deletion mapping and partially sequenced. This region encodes for a hypothetical 310-amino acid (aa) protein closely related to t...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1996.0029

    authors： Ankri S,Bouvier I,Reyes O,Predali F,Leblon G

    更新日期：1996-07-01 00:00:00

  • Carriage of type II toxin-antitoxin systems by the growing group of IncX plasmids.

    abstract：:The stable maintenance of certain plasmids in bacterial populations has contributed significantly to the current worldwide antibiotic resistance (AbR) emergency. IncX plasmids, long underestimated in this regard, have achieved recent notoriety for their roles in transmission of resistance to carbapenem and colistin, t...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2017.02.006

    authors： Bustamante P,Iredell JR

    更新日期：2017-05-01 00:00:00

  • New multi-purpose high copy number vector with greater mitotic stability for diverse applications in fission yeast Schizosaccharomyces pombe.

    abstract：:We have constructed a pUC19-based multipurpose ATG vector in Schizosaccharomyces pombe with higher copy number and mitotic stability possible with commonly used vectors. The vector, having an NdeI site in its polylinker to provide ATG site for expression, carries a greatly truncated version of URA3 gene, URA3m, of Sac...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2012.07.001

    authors： Verma HK,Singh J

    更新日期：2012-11-01 00:00:00

  • Restriction endonuclease mapping of R27 (TP117), an incompatibility group HI subgroup 1 plasmid from Salmonella typhimurium.

    abstract：:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(85)90058-7

    authors： Taylor DE,Brose EC

    更新日期：1985-01-01 00:00:00

  • Coupling sequences flanking Tn916 do not determine the affinity of binding of integrase to the transposon ends and adjacent bacterial DNA.

    abstract：:Coupling sequences are the 6 bp flanking the conjugative transposon Tn916 and are thought to play a role in determining the frequency of conjugative transposition. The affinity of binding of a chimeric protein, which consisted of maltose binding protein fused to the carboxy-terminal DNA binding domain of Tn916 integra...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1999.1440

    authors： Pethel B,Churchward G

    更新日期：2000-03-01 00:00:00

  • Construction of recombinant adenovirus vector containing a modified gene that codes for human hypoxia-inducible factor-1alpha without oxygen-dependent degradation domain.

    abstract：:The expression of hypoxia-inducible factor-1alpha (HIF-1alpha) in heart allografts is an important mechanism in response to ischemia/reperfusion (I/R) injury which represents the single major non-immunologic factor implicated in pathogenesis of chronic graft dysfunction (CGD). Adenoviral mediated overexpression of HIF...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2009.09.002

    authors： Wei L,Lu J,Feng L,Li S,Shan J,Li Y

    更新日期：2010-01-01 00:00:00

  • A novel spontaneous mutation of the bovine papillomavirus-1 genome.

    abstract：:A cell clone (cl.2) having an atypical transformed morphology was isolated from a murine C127 cell culture experimentally infected with a bovine papillomavirus type 1 (BPV-1) virion preparation. cl.2 cells exhibited minimal transformed characteristics and contained multiple copies of a BPV-1 plasmid with a molecular s...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(88)90008-x

    authors： Burnett S,Moreno-Lopez J,Pettersson U

    更新日期：1988-07-01 00:00:00

  • Sequences of three transfer RNAs from mosquito mitochondria.

    abstract：:The sequences of three transfer RNAs from mosquito cell mitochondria, tRNAArgUCG, tRNAAspGUC, and tRNAIleGAU, determined using a combination of rapid ladder and fingerprinting procedures are reported. These were compared with hamster mitochondrial tRNAArgUCG and tRNAAspGUC determined similarly, and a bovine mitochondr...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(83)90057-4

    authors： HsuChen CC,Cleaves GR,Dubin DT

    更新日期：1983-07-01 00:00:00

  • Mechanism of action of the Rep protein from the Dictyostelium Ddp2 plasmid family.

    abstract：:The two-hybrid system was used to show that the Rep proteins from three members of the Dictyostelium discoideum Ddp2 plasmid family, Ddp2, Ddp5, and Ddp6, form homomultimers but not heteromultimers when expressed in yeast cells. The results with deletion mutations suggest that multiple regions of the Rep proteins are ...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1999.1399

    authors： Shammat IM,Welker DL

    更新日期：1999-05-01 00:00:00

  • Integration-proficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains.

    abstract：:An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was developed. The method relies on two integration-proficient vectors, mini-CTX1 and mini-CTX2. These two vectors contain (1) a tetracycline (tet) selectable marker, (2) an oriT for conjugation-...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1999.1441

    authors： Hoang TT,Kutchma AJ,Becher A,Schweizer HP

    更新日期：2000-01-01 00:00:00

  • A selection system for unequal crossing-over: plasmid construction and initial studies in Escherichia coli.

    abstract：:Unequal crossing-over is involved in genetic duplication and deletion in such diverse genetic systems as Drosophila, bacteria, and animal viruses. It is proposed to be involved in the form of unequal sister chromatid exchange in gene amplification in cultured animal cells and during carcinogenesis. Studies of the proc...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(85)90042-3

    authors： Clevenger W,Reeves R,Pall ML

    更新日期：1985-05-01 00:00:00

  • The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX.

    abstract：:Analysis of the 50-kb R-plasmid pTP10 from the clinical isolate Corynebacterium xerosis M82B revealed that the erythromycin resistance gene, ermCX, is located on a 4524-bp composite transposable element, Tn5432. The ends of Tn5432 are identical, direct repeats of an insertion sequence, designated IS1249, encoding a pu...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1995.9995

    authors： Tauch A,Kassing F,Kalinowski J,Pühler A

    更新日期：1995-09-01 00:00:00

  • Development of an IPTG inducible expression vector adapted for Bacteroides fragilis.

    abstract：:The genus Bacteroides are gram-negative, obligate anaerobes indigenous to the gastrointestinal tract of humans and animals. The Bacteroides and other members of the Bacteroidetes phylum have diverged from the Proteobacteria. These organisms evolved a unique promoter structure for the initiation of transcription, hence...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2012.03.002

    authors： Parker AC,Jeffrey Smith C

    更新日期：2012-09-01 00:00:00

  • Cloning and sequence analysis of a novel insertion element from plasmids harbored by the carbofuran-degrading bacterium, Sphingomonas sp. CFO6.

    abstract：:Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1997.1289

    authors： Feng X,Ou LT,Ogram A

    更新日期：1997-01-01 00:00:00

  • The Clostridium perfringens chloramphenicol resistance transposon Tn4451 excises precisely in Escherichia coli.

    abstract：:Nucleotide sequence analysis of the Tn4451-deletion derivatives, pJIR47 and pJIR86, which were derived from Escherichia coli and Clostridium perfringens, respectively, showed that the deletion events that led to the formation of these plasmids were identical and precise. The results also showed that the termini of thi...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(88)90055-8

    authors： Abraham LJ,Rood JI

    更新日期：1988-03-01 00:00:00