Abstract：
:The two-hybrid system was used to show that the Rep proteins from three members of the Dictyostelium discoideum Ddp2 plasmid family, Ddp2, Ddp5, and Ddp6, form homomultimers but not heteromultimers when expressed in yeast cells. The results with deletion mutations suggest that multiple regions of the Rep proteins are involved in the multimerization. Electrophoretic mobility shift assays with heterologously expressed and purified Ddp2 Rep protein showed that it is a DNA binding protein. The nucleosomal organization of Ddp2 and Ddp6 in their inverted repeat and promoter regions was investigated. Analysis of mutants derived from the Ddp6 plasmid revealed that its Rep protein is required for nucleosome positioning (i.e., phasing) to occur in the promoter region. On the other hand, nucleosome positioning in the inverted repeat regions of both plasmids is not dependent on Rep protein but on either a feature of the DNA sequence or the binding of cellular factors, perhaps the Dictyostelium origin recognition complex. Rep protein is likely involved in transcription regulation and control of DNA replication, specifically amplification of plasmid at low copy numbers. The formation of homomultimers may be required for their regulatory activity.
journal_name
Plasmidjournal_title
Plasmidauthors
Shammat IM,Welker DLdoi
10.1006/plas.1999.1399keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
248-59issue
3eissn
0147-619Xissn
1095-9890pii
S0147-619X(99)91399-9journal_volume
41pub_type
杂志文章相关文献
PLASMID文献大全abstract：:Transposon-insertion mutants with vir- Ti plasmids were characterized and then used in complementation experiments. One of the mutants (LBA 1517) had a mutation in a newly discovered vir locus called virF. The virF mutation led to a strongly diminished virulence on tomato and tobacco, but not on certain other plant sp...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(84)90026-x
更新日期：1984-05-01 00:00:00
abstract：:Nucleotide sequence analysis of two cryptic plasmids, pRS2 (2544 bp) and pRS3 (3948 bp), from Oenococcus oeni revealed the presence in both of three major open reading frames with significant similarity to other small cryptic plasmids from O. oeni. The results suggest that those plasmids could be separated into two su...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.2001.1537
更新日期：2001-09-01 00:00:00
abstract：:The genus Bacteroides are gram-negative, obligate anaerobes indigenous to the gastrointestinal tract of humans and animals. The Bacteroides and other members of the Bacteroidetes phylum have diverged from the Proteobacteria. These organisms evolved a unique promoter structure for the initiation of transcription, hence...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2012.03.002
更新日期：2012-09-01 00:00:00
abstract：:A restriction enzyme map of the IncFI plasmid ColV2-K94 was generated using EcoRI, BamHI, HindIII, and XhoI; the genetic features of this element were then mapped from previous heteroduplex studies. ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(85)90046-0
更新日期：1985-05-01 00:00:00
abstract：:Lactococcus lactis ME2 can transfer a 46-kb plasmid, pTR2030, which encodes abortive phage infection (Hsp) and restriction/modification (R/M) activities. pTR2030 can be detected as a monomeric plasmid in transconjugants at low copy number, but not in ME2. pTR2030-specific probes were cloned and used to determine the l...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(91)90021-n
更新日期：1991-03-01 00:00:00
abstract：:Bacterial chromosomes segregate via a partition apparatus that employs a score of specialized proteins. The SMC complexes play a crucial role in the chromosome partitioning process by organizing bacterial chromosomes through their ATP-dependent chromatin-compacting activity. Recent progress in the composition of these...
journal_title：Plasmid
pub_type： 杂志文章,评审
doi：10.1016/j.plasmid.2005.08.004
更新日期：2006-03-01 00:00:00
abstract：:A cell clone (cl.2) having an atypical transformed morphology was isolated from a murine C127 cell culture experimentally infected with a bovine papillomavirus type 1 (BPV-1) virion preparation. cl.2 cells exhibited minimal transformed characteristics and contained multiple copies of a BPV-1 plasmid with a molecular s...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(88)90008-x
更新日期：1988-07-01 00:00:00
abstract：:A suppressor of the mutations which inhibit the establishment of lysogeny in Pseudomonas aeruginosa has been found to be encoded by P. aeruginosa plasmids of four separate incompatibility groups. The wide distribution of this phenotype (designated Sly for suppressor of lysogeny establishment deficiency) indicates that...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(84)90024-6
更新日期：1984-03-01 00:00:00
abstract：:Pinene is a monoterpene with wide industrial applications, especially as a promising high energy-density jet fuel. Traditional production of pinene on an industrial scale is material consumptive and has a low yield. As an alternative, microbial organisms have been engineered though advanced synthetic biological techni...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2019.102431
更新日期：2019-09-01 00:00:00
abstract：:Even though Vibrio cholerae is a well-known human pathogen, it is also a normal member of aquatic habitats. Within this environment it often forms biofilms on the chitin-containing exoskeleton of crustaceans and their molts. Chitin not only serves as nutrient source but also induces a developmental program called natu...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2010.08.001
更新日期：2010-11-01 00:00:00
abstract：:pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(91)90042-u
更新日期：1991-11-01 00:00:00
abstract：:A transient expression system has been developed to compare the relative efficiency of expression of various vaccinia virus DNA sequences containing transcriptional regulatory elements. A plasmid vector was constructed containing both the Escherichia coli galactokinase gene (galK) and the guanine phosphoribosyltransfe...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(87)90074-6
更新日期：1987-07-01 00:00:00
abstract：:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1994.1062
更新日期：1994-09-01 00:00:00
abstract：:A preliminary screening study of six Moraxella catarrhalis isolates from primary school children in the Netherlands identified a small 3.5 kb plasmid (pEMCJH03), containing four open reading frames, which encoded three mobilizing and one replicase protein. Insertion of a kanamycin containing transposon (yielding pEMCJ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2004.11.003
更新日期：2005-05-01 00:00:00
abstract：:The ability of some bacteria to grow in the presence of high concentrations of tellurium compounds has been recognized for almost 100 years. Since then, interest in this phenomenon has generated a slow but steady trickle of literature. In the past few years, the use of modern techniques in molecular biology has led to...
journal_title：Plasmid
pub_type： 杂志文章,评审
doi：10.1016/0147-619x(92)90006-v
更新日期：1992-01-01 00:00:00
abstract：:The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155....
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2019.01.002
更新日期：2019-03-01 00:00:00
abstract：:Bacillus mycoides, a member of the Bacillus cereus group of bacteria, can be easily distinguished from close species because of colony shape, made by filaments of cells, resembling fungal hyphae, curving clock- or counterclockwise depending on the strain. Two plasmids, one from a strain curving to the right (pDx14.2),...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2005.05.002
更新日期：2005-11-01 00:00:00
abstract：:Bacterial plasmids and phages encode the synthesis of toxic molecules that inhibit protozoan predation. One such toxic molecule is violacein, a purple pigmented, anti-tumour antibiotic produced by the Gram-negative soil bacterium Chromobacterium violaceum. In the current experiments a range of Escherichia coli K12 str...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2011.07.006
更新日期：2011-09-01 00:00:00
abstract：:Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurring compatible members of the Dictyostelium Ddp1 plasmid family found in the same wild isolate strain. The nucleotide sequences of Dmp1 (5983 bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading frames (ORFs) similar to the G1 and G5...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1994.1013
更新日期：1994-03-01 00:00:00
abstract：:pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper s...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2015.07.003
更新日期：2015-09-01 00:00:00
abstract：:The complete nucleotide sequence of a plasmid, designated pRJF1, isolated from a rumen bacterium of the genus Butyrivibrio, has been determined. pRJF1 is a small plasmid (2631 bp) which shows the high AT content (64%) typical of plasmids from gram-positive organisms. Computer analysis of sequence data revealed two maj...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1993.1008
更新日期：1993-01-01 00:00:00
abstract：:Cloning vectors (pFD1001, pFD1192, pFD1194, and pFD1212) were constructed by extension of the host range of a 7.2-kb Rhizobium meliloti cryptic plasmid (pRM1132f) with the ColE1-based plasmids, pBR322, pACYC177, pACYC 184, pSUP301, or pHC179; mobilization was facilitated by introduction of the oriT region from pRK2, a...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1995.1024
更新日期：1995-05-01 00:00:00
abstract：:Almost 200 scientists from America, Europe, Asia, Australia, and Africa participated in the Plasmid Biology 2004 meeting, which was organized between 15th and 21st September 2004 in Kanoni (Corfu island), Greece. Various aspects of biology of plasmids and other mobile genetic elements were discussed during the meeting...
journal_title：Plasmid
pub_type：
doi：10.1016/j.plasmid.2004.10.002
更新日期：2005-01-01 00:00:00
abstract：:Ten strains of Rhodopseudomonas sphaeroides were analyzed by agarose gel electrophoresis for plasmid DNA content and, by filter-hybridizations, for their molecular relationships. All strains examined contained at least one plasmid. Several strains carried as many as six different plasmid species with sizes ranging fro...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(84)90005-2
更新日期：1984-01-01 00:00:00
abstract：:A new class of binary vectors has been constructed, containing the origin of replication of the W-type plasmid Sa. These vectors are designed to express foreign genes in plants under control of the TR gene 2' promoter or the promoter of a light-inducible ribulose-1,5-bisphosphate carboxylase small subunit gene of Arab...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(88)90043-1
更新日期：1988-05-01 00:00:00
abstract：:A new insertion variant of IS231 family, designated ISBth4, was identified from Bacillus thuringiensis MEX312. ISBth4 is 2046bp in length and is delimited by two 17bp inverted repeats (IR) with one mismatch, flanked by two perfect 11bp direct repeats (DR). ISBth4 contains two open reading frames (ORFs), ORF1 and ORF2,...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2009.10.002
更新日期：2010-01-01 00:00:00
abstract：:In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restric...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(90)90007-y
更新日期：1990-11-01 00:00:00
abstract：:In order to study the in vivo assembly of chromatin on prokaryotic DNA templates, we have transformed yeast cells with plasmids pAJ50 and pRB58, which contain pBR322 sequences. In both cases nucleosomes are assembled in vivo on pBR322 DNA, although the nucleosomes are not homogeneous in size. To explore whether there ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(89)90054-1
更新日期：1989-03-01 00:00:00
abstract：:The promiscuous IncPα plasmids RK2 and R995 encode a broad-host-range partition system, whose essential components include the incC and korB genes and a DNA site (O(B)) to which the korB product binds. IncC2, the smaller of the two incC products, is sufficient for stabilization of R995ΔincC. It is a member of the type...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2012.04.003
更新日期：2012-09-01 00:00:00
abstract：:Nocardia brasiliensis, is a bacteria that lives as saprophyte in soil and causes a disease called actinomycetoma in both human and animals. Nocardia brasiliensis is an intracellular, facultative bacterium that replicates and survives within host macrophages. The mechanisms involved in the evasion of the microbicidal a...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2010.09.005
更新日期：2011-01-01 00:00:00
