Abstract:
:Ten strains of Rhodopseudomonas sphaeroides were analyzed by agarose gel electrophoresis for plasmid DNA content and, by filter-hybridizations, for their molecular relationships. All strains examined contained at least one plasmid. Several strains carried as many as six different plasmid species with sizes ranging from 42 to 140 kilobases (kb). Those larger than 89 kb showed extensive homology with each other; the 42-kb plasmid of R. sphaeroides strain 2.4.1 was homologous to the smaller plasmid DNA of three other strains. A partial map of the 42-kb plasmid derived from R. sphaeroides 2.4.1 was prepared by analysis of restriction endonuclease digests. Cross-hybridization among the large plasmids indicated that those present in any one strain of R. sphaeroides showed homology to one or more of the large plasmids detected in strains L and 2.4.1.
journal_name
Plasmidjournal_title
Plasmidauthors
Fornari CS,Watkins M,Kaplan Sdoi
10.1016/0147-619x(84)90005-2subject
Has Abstractpub_date
1984-01-01 00:00:00pages
39-47issue
1eissn
0147-619Xissn
1095-9890pii
0147-619X(84)90005-2journal_volume
11pub_type
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