Complete nucleotide sequence of a quinolone resistance gene (qnrS2) carrying plasmid of Aeromonas hydrophila isolated from fish.

abstract：:Since reduced metronidazole causes DNA damage, resistance to metronidazole was used as a selection method for the cloning of Bacteroides fragilis genes affecting DNA repair mechanisms in Escherichia coli. Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli AB1157 and uvrA, B, and...

journal_title：Plasmid

authors： Wehnert GU,Abratt VR,Goodman HJ,Woods DR

更新日期：1990-03-01 00:00:00

abstract：:The cloning of the finO region from the antibiotic-resistance plasmid R6-5 is reported. On the basis of DNA deletion analysis and Tn5 transposon insertional mutagenesis of finO+ chimeric plasmids, finO has been located within the coordinates 94.0-94.85 on the R6-5 map. A 32,000-Da polypeptide (32K), which is encoded w...

journal_title：Plasmid

authors： Cheah KC,Ray A,Skurray R

更新日期：1984-11-01 00:00:00

abstract：:Isolates of Rhodococcus equi from pneumonic foals possess an 85- or 90-kb virulence-associated plasmid. A prominent, thermoregulated surface antigen, VapA, encoded by these plasmids is thought to be important in virulence. A 135-kb fragment containing the origin of replication of R. equi strain 103 virulence-associate...

journal_title：Plasmid

authors： Zheng H,Tkachuk-Saad O,Prescott JF

更新日期：1997-01-01 00:00:00

abstract：:Polymerase chain reaction (PCR) is one of the most powerful tools in cloning genes. For the direct cloning of PCR products, T-vectors, which contain complementary 3'-thymidine overhangs, are widely used. In the present study, we developed a plasmid, pNB-T, which was constructed by cloning an XcmI cassette with a suffi...

journal_title：Plasmid

authors： Jo C,Jo SA

更新日期：2001-01-01 00:00:00

abstract：:A simple mathematical model of drug-induced plasmid elimination (curing) considering density-dependent growth rates and plasmid transfers is presented. It describes nonlinear population dynamics of conjugative plasmids during in vitro curing experiments in batch culture. The model was tested on kinetics of acridine or...

journal_title：Plasmid

authors： Sýkora P,Foltýnova Z,Smitálova K

更新日期：1989-03-01 00:00:00

abstract：:Taking advantage of the plaque morphology method, we detected two single-stranded initiation (ssi) signals in the plasmid pSY343; one was in the 170-nucleotide (nt) EcoRV-ThaI segment (170P), and the other was in the 93-nt DraI-FnuDII segment (93F), which were designated as ssiA and ssiB, respectively. We cloned the t...

journal_title：Plasmid

authors： Bahk JD,Kioka N,Sakai H,Komano T

更新日期：1988-11-01 00:00:00

abstract：:Rolling circle-replicating plasmids constitute a vast family that is particularly abundant in, but not exclusive of, Gram-positive bacteria. These plasmids are constructed as cassettes that harbor genes involved in replication and its control, mobilization, resistance determinants and one or two origins of lagging str...

journal_title：Plasmid

authors： Lorenzo-Díaz F,Fernández-López C,Garcillán-Barcia MP,Espinosa M

更新日期：2014-07-01 00:00:00

abstract：:The joining of DNA ends was compared in the established CV1 cell line and in African green monkey kidney primary cells, from which the CV1 cell line was established. Using a linear SV40 genome that carries a terminal repeat of 526 nucleotide pairs, we measured the efficiency of circularization by end joining relative ...

journal_title：Plasmid

authors： Zheng H,Chang XB,Wilson JH

更新日期：1989-09-01 00:00:00

abstract：:Several staphylococcal plasmids (26-45 kb) carry all three streptogramin-resistance (Sg(R)) genes, vat, vgb, and vga. Seven such plasmids harbored by independent strains belonging to three taxa (Staphylococcus aureus, S. simulans, and S. cohnii subsp. urealyticum) were compared and the deleted derivative of one of the...

journal_title：Plasmid

authors： Allignet J,El Solh N

更新日期：1999-09-01 00:00:00

abstract：:The structure of several T-DNAs of Agrobacterium tumefaciens was determined by molecular cloning and Southern hybridization. The T-DNAs cloned in Escherichia coli vectors from four different nopaline type strains (PyTE1, PO31, PO22, and AKE10) showed various sizes of restriction enzyme fragments. Comparative analysis ...

journal_title：Plasmid

authors： Wabiko H,Kagaya M,Sano H

更新日期：1991-01-01 00:00:00

abstract：:The imidazole and triazole fungicides inhibit cytochrome P450 14 alpha-lanosterol demethylase (P45014DM) implicated in the ergosterol biosynthesis pathway, which is specific to fungi and yeasts. Two plasmids were obtained which allow triazole and imidazole resistance in Saccharomyces cerevisiae. The low copy number pl...

journal_title：Plasmid

authors： Doignon F,Aigle M,Ribereau-Gayon P

更新日期：1993-11-01 00:00:00

abstract：:We report herein the isolation and molecular characterization of pBGR1, the first native plasmid isolated from the genus Bartonella. Cloning and sequencing revealed a 2725-base pair (bp) cryptic plasmid comprising two open reading frames of considerable length, which were designated rep and mob. The regions containing...

journal_title：Plasmid

authors： Seubert A,Falch C,Birtles RJ,Schulein R,Dehio C

更新日期：2003-01-01 00:00:00

abstract：:Four plasmids encoding restriction and modification (R/M) systems are described that are different in the specificity of their restrictive activity toward the small isometric phage p2 and prolate phage c2. The R/M plasmids were cotransformed into Lactococcus lactis MG1363 with pVS2, encoding resistance to chlorampheni...

journal_title：Plasmid

authors： Josephsen J,Klaenhammer T

更新日期：1990-01-01 00:00:00

abstract：:The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...

journal_title：Plasmid

authors： Bartosik D,Baj J,Piechucka E,Waker E,Wlodarczyk M

更新日期：2002-09-01 00:00:00

abstract：:Almost 200 scientists from America, Europe, Asia, Australia, and Africa participated in the Plasmid Biology 2004 meeting, which was organized between 15th and 21st September 2004 in Kanoni (Corfu island), Greece. Various aspects of biology of plasmids and other mobile genetic elements were discussed during the meeting...

journal_title：Plasmid

authors： Wegrzyn G

更新日期：2005-01-01 00:00:00

abstract：:We report here the molecular characterization of pFNL10, a 3990-bp cryptic plasmid of Francisella novicida-like F6168. The plasmid was maintained in F. novicida Utah 112 and F. tularensis LVS strains. We sequenced the entire plasmid and found six open reading frames (ORFs)-ORF1, ORF2, ORF3, ORF4, ORF5, and ORFm. ORF3,...

journal_title：Plasmid

authors： Pomerantsev AP,Golovliov IR,Ohara Y,Mokrievich AN,Obuchi M,Norqvist A,Kuoppa K,Pavlov VM

更新日期：2001-11-01 00:00:00

abstract：:This review provides a historical perspective of the major findings that contributed to our current understanding of plasmid rolling-circle (RC) replication. Rolling-circle-replicating (RCR) plasmids were discovered approximately 20 years ago. The first of the RCR plasmids to be identified were native to Gram-positive...

journal_title：Plasmid

authors： Khan SA

更新日期：2005-03-01 00:00:00

abstract：:pBTK445 is a newly described large (∼60Kb), low-copy number, conjugative plasmid indigenous to the sulfur-chemolithoautotroph Advenella kashmirensis. Based on its minimal replication region, a shuttle vector, pBTKS was constructed which can be used for diverse Alcaligenaceae members. The construct was found to be stab...

journal_title：Plasmid

authors： Dam B

更新日期：2011-03-01 00:00:00

abstract：:Heterologous expression in Escherichia coli is often limited by yield and solubility of the foreign protein in the bacterial cytoplasm. In many cases, overexpression also results in growth inhibition. In order to produce retroviral proteins that are especially difficult to overexpress in E. coli, we designed a set of ...

journal_title：Plasmid

authors： Valverde V,Duplan H,Knibiehler M,Masson JM

更新日期：1994-07-01 00:00:00

abstract：:The structural and functional properties of mercury resistance determinants cloned from a series of independently isolated conjugative plasmids were compared with those of the prototype HgR determinants from Tn501 and plasmid R100 (containing Tn21). Restriction endonuclease mapping classified the HgR determinants into...

journal_title：Plasmid

authors： Jobling MG,Peters SE,Ritchie DA

更新日期：1988-09-01 00:00:00

abstract：:Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons. Amplification products were obtained from 20 of the hybridization-positive strains. A single dfrA7 cassette was identified in 18 of the isolates and an integron with two cassettes (aadB-aadA4) ...

journal_title：Plasmid

authors： Segal H,Thomas R,Gay Elisha B

更新日期：2003-03-01 00:00:00

abstract：:We have modified an E. coli-staphylococcal shuttle vector for use in the general cloning and expression of genes from pathogenic staphylococci in Staphylococcus carnosus. As S. carnosus is non-pathogenic, this expression system will facilitate the study of the roles of individual gene products in the disease process. ...

journal_title：Plasmid

authors： Williams RJ,Nair SP,Henderson B,Holland KT,Ward JM

更新日期：2002-05-01 00:00:00

abstract：:Most Vibrio fischeri strains isolated from the Euprymna scolopes light organ carry plasmids, often including both a large (>40kb) plasmid, and one or more small (<12kb) plasmids. The large plasmids share homology with pES100, which is the lone plasmid in V. fischeri type strain ES114. pES100 appears to encode a conjug...

journal_title：Plasmid

authors： Dunn AK,Martin MO,Stabb EV

更新日期：2005-09-01 00:00:00

abstract：:To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...

journal_title：Plasmid

authors： Domingues E,Brillet T,Vasseur C,Agier V,Marden MC,Baudin-Creuza V

更新日期：2009-01-01 00:00:00

abstract：:A set of low copy number plasmid vectors for mammalian gene expression has been constructed. These vectors are derived from the previously described bacterial low copy number expression vectors, pWSK29 and pWKS30, which are present at six to eight copies per cell. The new plasmids also have the following useful proper...

journal_title：Plasmid

authors： Fontaine SL,Firth SD,Lockhart PJ,Paynter JA,Mercer JF

更新日期：1998-01-01 00:00:00

abstract：:Corynebacterium glutamicum is an industrial microorganism for production of amino acids. However, the metabolic engineering in C. glutamicum has been retarded due to lack of suitable vectors. In this study, we have constructed a shuttle vector pDXW-10 which harbors a large multiple cloning site suitable for cloning mu...

journal_title：Plasmid

authors： Xu D,Tan Y,Shi F,Wang X

更新日期：2010-09-01 00:00:00

abstract：:pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...

journal_title：Plasmid

authors： Chiang CS,Bremer H

更新日期：1991-11-01 00:00:00

abstract：:The CoIV plasmids are large virulence plasmids of the incompatibility group IncFI. We have obtained the genetic maps of two of the most studied CoIV plasmids, pCoIV-B188 and pCoIV-K30, according to restriction enzyme sites and landmark phenotypic characteristics such as colicin V, the aerobactin iron uptake system, th...

journal_title：Plasmid

authors： Waters VL,Perez-Casal JF,Crosa JH

更新日期：1989-11-01 00:00:00

abstract：:The rapidly advancing field of plant synthetic biology requires transforming plants with multiple genes. This has sparked a growing interest in flexible plant transformation vectors, which can be used for multi-gene transformations. We have developed a novel binary vector series, named the PC-GW series (GenBank: KP826...

journal_title：Plasmid

authors： Dalal J,Yalamanchili R,La Hovary C,Ji M,Rodriguez-Welsh M,Aslett D,Ganapathy S,Grunden A,Sederoff H,Qu R

更新日期：2015-09-01 00:00:00

abstract：:pIP501 and pAM beta 1 are promiscuous plasmids from gram-positive bacteria which carry structurally and genetically related replication functions (L. Jannière, A. Gruss and S.D. Ehrlich, 1993, in "Bacillus subtilis and Other Gram-positive Bacteria: Biochemistry, Physiology, and Molecular Genetics (A.L. Sonenshein, J.A...

journal_title：Plasmid

authors： Pujol C,Ehrlich SD,Jannière L

更新日期：1994-01-01 00:00:00