Abstract:
:The structure of several T-DNAs of Agrobacterium tumefaciens was determined by molecular cloning and Southern hybridization. The T-DNAs cloned in Escherichia coli vectors from four different nopaline type strains (PyTE1, PO31, PO22, and AKE10) showed various sizes of restriction enzyme fragments. Comparative analysis of the restriction maps revealed that the T-DNAs were composed of three distinct structural domains: (1) the region proximal to the right border (Domain I) containing the portion essential for tumorigenicity, (2) the proximity to the left border (Domain II), and (3) the region between the two domains (Domain III) to both of which no functional assignments have yet been made. The restriction map indicated that the Domains I and II were conserved in the most clones, including the well-characterized T37 T-DNA. The only exception was AKK1 (obtained from AKE10) which differed in Domain I. In the Domain III, insertions of 1.5- or 1.6-kb DNA were found in four clones, whereas an additional 2.5-kb insertion was found in one clone (PO22P1). The individual T-DNAs including Domain III with insertions was demonstrated in petunia and poplar tumors induced by the referred A. tumefaciens strains. However, resulting tumors differed in morphology and growth. These results suggest that the length polymorphism of the nopaline type T-DNA can be accounted by DNA insertions, and that diverse T-DNAs reflect their different roles in tumorigenicity.
journal_name
Plasmidjournal_title
Plasmidauthors
Wabiko H,Kagaya M,Sano Hdoi
10.1016/0147-619x(91)90002-esubject
Has Abstractpub_date
1991-01-01 00:00:00pages
3-15issue
1eissn
0147-619Xissn
1095-9890pii
0147-619X(91)90002-Ejournal_volume
25pub_type
杂志文章相关文献
PLASMID文献大全abstract::Some strains of the Enterobacteriaceae Serratia entomophila and Serratia proteamaculans cause amber disease in the grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. The genes responsible for this disease reside on a large, 155-kb plasmid designated amber disease-as...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(03)00062-3
更新日期:2003-11-01 00:00:00
abstract::The mercury(II)-resistance transposons Tn502 and Tn512 were sequenced and shown to be members of the Tn5053 family. They are currently the sole representatives from the clinical setting and were obtained from geographically disparate Pseudomonas aeruginosa strains. The family is comprised of six novel transposons that...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.08.003
更新日期:2011-01-01 00:00:00
abstract::Rolling circle-replicating plasmids constitute a vast family that is particularly abundant in, but not exclusive of, Gram-positive bacteria. These plasmids are constructed as cassettes that harbor genes involved in replication and its control, mobilization, resistance determinants and one or two origins of lagging str...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2014.05.004
更新日期:2014-07-01 00:00:00
abstract::Tn4555 is a 12.1-kb Bacteroides antibiotic resistance transposon representative of a novel class of transmissible genetic elements that can be transferred by resident conjugative tetracycline resistance transposons (Tc(r)-elements) but are not capable of self-transfer. Previously it was shown that Tn4555 transposes by...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1401
更新日期:1999-07-01 00:00:00
abstract::This review provides a historical perspective of the major findings that contributed to our current understanding of plasmid rolling-circle (RC) replication. Rolling-circle-replicating (RCR) plasmids were discovered approximately 20 years ago. The first of the RCR plasmids to be identified were native to Gram-positive...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2004.12.008
更新日期:2005-03-01 00:00:00
abstract::Single plasmids designed to express the six human type I steroid hormone receptors and detect signaling activity are described in this report. These stably replicating plasmids reported ligand-induced transcriptional activation via lacZ assays in Baker's yeast (Saccharomyces cerevisiae). The ligand concentrations need...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.11.003
更新日期:2010-03-01 00:00:00
abstract::To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2008.09.006
更新日期:2009-01-01 00:00:00
abstract::The galactose operon of Escherichia coli K-12 has been used as a phenotypic marker for miniplasmids derived in vitro from R plasmids representing six incompatibility groups. This has enabled the development of a rapid incompatibility typing scheme in which the miniplasmids are used as incompatibility exemplars, their ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(84)90029-5
更新日期:1984-05-01 00:00:00
abstract::An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was developed. The method relies on two integration-proficient vectors, mini-CTX1 and mini-CTX2. These two vectors contain (1) a tetracycline (tet) selectable marker, (2) an oriT for conjugation-...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1441
更新日期:2000-01-01 00:00:00
abstract::A collection of 111 commensal Escherichia coli isolated from 84 faecal samples from healthy Australian adults were screened using PCR-based replicon typing. Each isolate represented a distinct strain found in a particular faecal sample. Fifty-one isolates were resistant to one or more of 12 antibiotics tested. FII and...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.03.005
更新日期:2015-07-01 00:00:00
abstract::Glycopeptide resistant Enterococcus faecium (GREF) persists on Norwegian poultry farms despite the ban on the growth promoter avoparcin. The biological basis for long-term persistence of avoparcin resistance is not fully understood. This study presents the complete DNA sequence of the E. faecium R-plasmid pVEF3 and fu...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2008.04.002
更新日期:2008-07-01 00:00:00
abstract::Tn4527 was isolated from a Salmonella typhimurium strain obtained in Brazil. Its size is 19.6 kb and it carries resistance to trimethoprim, spectinomycin, and streptomycin, as in the case of Tn7 (14 kb). A restriction analysis of the transposon shows regions of similarity to Tn7 mixed with extra DNA. The 2.6-kb and 2....
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(89)90009-7
更新日期:1989-11-01 00:00:00
abstract::A combined physical and functional map of plasmid RP4 is presented including the sites for 18 restriction endonucleases. Several cleavage sites of SphI, BalI, and ApaI are suitable for the dissection of the transfer gene regions. Recombinant plasmids containing RP4 SphI fragments were constructed to assist in localizi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(83)90047-1
更新日期:1983-11-01 00:00:00
abstract::To study the transcriptional activity of the HIV-1 LTR, we constructed a vector containing Renilla and Firefly luciferase genes under the control of the LTR (wild-type or mutated version) and oriented in a manner that allowed them to be transcribed in opposite directions. We found that the HIV-1 LTR acted as a bidirec...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2014.06.001
更新日期:2014-07-01 00:00:00
abstract::Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2002.1565
更新日期:2002-03-01 00:00:00
abstract::A new insertion variant of IS231 family, designated ISBth4, was identified from Bacillus thuringiensis MEX312. ISBth4 is 2046bp in length and is delimited by two 17bp inverted repeats (IR) with one mismatch, flanked by two perfect 11bp direct repeats (DR). ISBth4 contains two open reading frames (ORFs), ORF1 and ORF2,...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.10.002
更新日期:2010-01-01 00:00:00
abstract::The nucleotide sequence of two novel plasmids isolated from the extreme thermophilic anaerobic bacterium Anaerocellum thermophilum DSM6725 (A. thermophilum), growing optimally at 70 degrees C, has been determined. pBAS2 was found to be a 3653 bp plasmid with a GC content of 43%, and the sequence revealed 10 open readi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.06.001
更新日期:2004-09-01 00:00:00
abstract::A phagemid vector library for cloning DNA with four nucleotide 5' or 3' overhangs has been constructed. This library is based on the pT7T3 vector (Pharmacia) which is a modification of the phagemid pTZ18U vector. We have chosen pT7T3 as the parent vector because it can be used for Sanger's dideoxy sequencing and for t...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(91)90038-x
更新日期:1991-07-01 00:00:00
abstract::The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155....
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2019.01.002
更新日期:2019-03-01 00:00:00
abstract::The structural and functional properties of mercury resistance determinants cloned from a series of independently isolated conjugative plasmids were compared with those of the prototype HgR determinants from Tn501 and plasmid R100 (containing Tn21). Restriction endonuclease mapping classified the HgR determinants into...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(88)90013-3
更新日期:1988-09-01 00:00:00
abstract::RNA interference is a widely used tool for analysis of gene function in mammalian cells. Stable knockdown of specific target genes can be maintained in cell lines and live organisms using vector-based delivery of short hairpins (shRNAs) driven by RNA polymerase III promoters. Here we describe a vector incorporating th...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2005.11.008
更新日期:2006-05-01 00:00:00
abstract::Several staphylococcal plasmids (26-45 kb) carry all three streptogramin-resistance (Sg(R)) genes, vat, vgb, and vga. Seven such plasmids harbored by independent strains belonging to three taxa (Staphylococcus aureus, S. simulans, and S. cohnii subsp. urealyticum) were compared and the deleted derivative of one of the...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1412
更新日期:1999-09-01 00:00:00
abstract::Here we present evidence that the cryptic 5.6-kb plasmid found in certain strains of Streptococcus mutans is not involved in mutacin production. This evidence comes from demonstrating similarities between a plasmid-less strain T8 and a group II plasmid strain UA96. Both produce what appears to be an identical mutacin ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(90)90013-3
更新日期:1990-09-01 00:00:00
abstract::Transcription of the 7.5-kb cryptic plasmid of Chlamydia trachomatis serovar L2 was investigated. Faint, diffuse transcripts of about 1.6 and 2.2 kb and intense short transcripts of about 250 and 430 bases were identified by Northern blot analysis. The short transcripts were found to have a common 5' end corresponding...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90056-g
更新日期:1992-11-01 00:00:00
abstract::In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restric...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(90)90007-y
更新日期:1990-11-01 00:00:00
abstract::Most Acinetobacter plasmids are genus specific but their properties have not been investigated. Small plasmids with Rep_3 family replication initiation proteins and iterons are common in Acinetobacter baumannii and often carry antibiotic resistance genes and toxin-antitoxin systems. A RepAci1 plasmid, carrying the car...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2019.04.002
更新日期:2019-05-01 00:00:00
abstract::Heterologous expression in Escherichia coli is often limited by yield and solubility of the foreign protein in the bacterial cytoplasm. In many cases, overexpression also results in growth inhibition. In order to produce retroviral proteins that are especially difficult to overexpress in E. coli, we designed a set of ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1041
更新日期:1994-07-01 00:00:00
abstract::Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurring compatible members of the Dictyostelium Ddp1 plasmid family found in the same wild isolate strain. The nucleotide sequences of Dmp1 (5983 bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading frames (ORFs) similar to the G1 and G5...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1013
更新日期:1994-03-01 00:00:00
abstract::Fusobacterium nucleatum is a Gram-negative anaerobic rod found in dental plaque biofilms, and is an opportunistic pathogen implicated in periodontitis as well as a wide range of systemic abscesses and infections. Genomic analyses of F. nucleatum indicate considerable genetic diversity and a prominent role for horizont...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.09.001
更新日期:2010-01-01 00:00:00
abstract::In order to study the in vivo assembly of chromatin on prokaryotic DNA templates, we have transformed yeast cells with plasmids pAJ50 and pRB58, which contain pBR322 sequences. In both cases nucleosomes are assembled in vivo on pBR322 DNA, although the nucleosomes are not homogeneous in size. To explore whether there ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(89)90054-1
更新日期:1989-03-01 00:00:00