The use of mini-Gal plasmids for rapid incompatibility grouping of conjugative R plasmids.

abstract：:We have examined the extent to which the degradative plasmids SAL, NAH, and TOL of the Inc P-9 incompatibility group share common DNA sequences. The homology we observe using 32P-labeled SAL and NAH DNA probes can be assigned to six regions of the TOL (pWWO) restriction endonuclease cleavage map. At least three of the...

journal_title：Plasmid

authors： Lehrbach PR,McGregor I,Ward JM,Broda P

更新日期：1983-09-01 00:00:00

abstract：:A 50,709-bp cryptic plasmid isolated from a marine Micrococcus has been sequenced and found to contain a number of putative mobile genetic elements. The coding regions for 11 putative transposases comprise approximately 17% of the total plasmid sequence. The majority of these transposases are located within a 13-kb cl...

journal_title：Plasmid

authors： Zhong Z,Caspi R,Mincer T,Helinski D,Knauf V,Boardman K,Wilkinson JE,Shea T,DeLoughery C,Toukdarian A

更新日期：2002-01-01 00:00:00

abstract：:A procedure for the isolation of closed circular mitochondrial DNA (mtDNA) from cells permeabilized with digitonin is described. Compared to the standard procedure in which cells are broken after osmotic swelling, the digitonin-based procedure is more consistent and results in higher mtDNA yields. ...

journal_title：Plasmid

authors： Howell N,Nalty MS,Appel J

更新日期：1986-07-01 00:00:00

abstract：:The complete nucleotide sequence of the cryptic plasmid pCC1 from Corynebacterium callunae (4109 bp) was determined. DNA sequence analysis revealed five open reading frames longer than 200 bp. One of the deduced polypeptides showed homology with the Rep proteins encoded by plasmids of the pIJ101/pJV1 family of plasmid...

journal_title：Plasmid

authors： Venkova-Canova T,Pátek M,Nesvera J

更新日期：2004-01-01 00:00:00

abstract：:Analysis of the 50-kb R-plasmid pTP10 from the clinical isolate Corynebacterium xerosis M82B revealed that the erythromycin resistance gene, ermCX, is located on a 4524-bp composite transposable element, Tn5432. The ends of Tn5432 are identical, direct repeats of an insertion sequence, designated IS1249, encoding a pu...

journal_title：Plasmid

authors： Tauch A,Kassing F,Kalinowski J,Pühler A

更新日期：1995-09-01 00:00:00

abstract：:The linear plasmid SCP1 of Streptomyces coelicolor A3(2) is one of the genetically more studied linear streptomycete replicons. Although the genetics of SCP1 and its interaction with the host chromosome have been analyzed for nearly three decades no information exists on its replication. With the help of an ordered co...

journal_title：Plasmid

authors： Redenbach M,Bibb M,Gust B,Seitz B,Spychaj A

更新日期：1999-11-01 00:00:00

abstract：:We report the characterization of Tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the R-plasmid pGD10. Tn602 is highly homologous to the previously characterized Tn903, present on the R-plasmid R6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-I (hom...

journal_title：Plasmid

authors： Stibitz S,Davies JE

更新日期：1987-05-01 00:00:00

abstract：:Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurring compatible members of the Dictyostelium Ddp1 plasmid family found in the same wild isolate strain. The nucleotide sequences of Dmp1 (5983 bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading frames (ORFs) similar to the G1 and G5...

journal_title：Plasmid

authors： Kiyosawa H,Hughes JE,Welker DL

更新日期：1994-03-01 00:00:00

abstract：:Plasmid RK2 codes for two species of the replication initiator protein TrfA (33 and 44 kDa). Both polypeptides are strongly associated with membrane fractions of Escherichia coli host cells (W. Firshein and P. Kim, Mol. Microbiol. 23, 1-10, 1997). We investigated the role of a 12-amino-acid hydrophobic region (HR) in ...

journal_title：Plasmid

authors： Kim PD,Rosche TM,Firshein W

更新日期：2000-05-01 00:00:00

abstract：:In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restric...

journal_title：Plasmid

authors： Labes G,Simon R

更新日期：1990-11-01 00:00:00

abstract：:Cloning vectors (pFD1001, pFD1192, pFD1194, and pFD1212) were constructed by extension of the host range of a 7.2-kb Rhizobium meliloti cryptic plasmid (pRM1132f) with the ColE1-based plasmids, pBR322, pACYC177, pACYC 184, pSUP301, or pHC179; mobilization was facilitated by introduction of the oriT region from pRK2, a...

journal_title：Plasmid

authors： Froissard D,Bromfield ES,Whitwill S,Barran LR

更新日期：1995-05-01 00:00:00

abstract：:Bacterial plasmids and phages encode the synthesis of toxic molecules that inhibit protozoan predation. One such toxic molecule is violacein, a purple pigmented, anti-tumour antibiotic produced by the Gram-negative soil bacterium Chromobacterium violaceum. In the current experiments a range of Escherichia coli K12 str...

journal_title：Plasmid

authors： Ahmetagic A,Philip DS,Sarovich DS,Kluver DW,Pemberton JM

更新日期：2011-09-01 00:00:00

abstract：:The plasmid pMSP3535 is a popular vector for nisin-inducible expression of heterologous genes in lactic acid bacteria. However, the maximum protein expression level achievable with pMSP3535 is relatively low. In an effort to increase expression we modified pMSP3535 to create a high expression variant termed pMSP3535H2...

journal_title：Plasmid

authors： Kim JH,Mills DA

更新日期：2007-11-01 00:00:00

abstract：:Avirulence gene D (avrD) in strain PT23 of Pseudomonas syringae pv. tomato (Pst) specifies the production of syringolides, which are elicitors of plant defense reactions. An 83-kb indigenous plasmid (pPT23B) that carries avrD has been mapped and characterized and a putative par region was identified. pPT23B contains a...

journal_title：Plasmid

authors： Murillo J,Shen H,Gerhold D,Sharma A,Cooksey DA,Keen NT

更新日期：1994-05-01 00:00:00

abstract：:The 4.2-kb cryptic plasmid pVA380-1 has been used as a vector for the cloning of antibiotic resistance genes directly in streptococci, and in the construction of Escherichia coli/Streptococcus shuttle vectors. The results of subcloning experiments located the basic replicon of pVA380-1 within a 2.5-kb region. The nucl...

journal_title：Plasmid

authors： LeBlanc DJ,Lee LN,Abu-Al-Jaibat A

更新日期：1992-09-01 00:00:00

abstract：:Even though Vibrio cholerae is a well-known human pathogen, it is also a normal member of aquatic habitats. Within this environment it often forms biofilms on the chitin-containing exoskeleton of crustaceans and their molts. Chitin not only serves as nutrient source but also induces a developmental program called natu...

journal_title：Plasmid

authors： De Souza Silva O,Blokesch M

更新日期：2010-11-01 00:00:00

abstract：:Glycopeptide resistant Enterococcus faecium (GREF) persists on Norwegian poultry farms despite the ban on the growth promoter avoparcin. The biological basis for long-term persistence of avoparcin resistance is not fully understood. This study presents the complete DNA sequence of the E. faecium R-plasmid pVEF3 and fu...

journal_title：Plasmid

authors： Sletvold H,Johnsen PJ,Hamre I,Simonsen GS,Sundsfjord A,Nielsen KM

更新日期：2008-07-01 00:00:00

abstract：:The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that...

journal_title：Plasmid

authors： Chakravartty V,Cronan JE

更新日期：2015-09-01 00:00:00

abstract：:The expression of hypoxia-inducible factor-1alpha (HIF-1alpha) in heart allografts is an important mechanism in response to ischemia/reperfusion (I/R) injury which represents the single major non-immunologic factor implicated in pathogenesis of chronic graft dysfunction (CGD). Adenoviral mediated overexpression of HIF...

journal_title：Plasmid

authors： Wei L,Lu J,Feng L,Li S,Shan J,Li Y

更新日期：2010-01-01 00:00:00

abstract：:The effects of the single-strand origins (SSOs) of plasmid pMV158 on (i) the conversion of its single-stranded (ss) replication intermediates to double-stranded (ds) plasmid DNA and (ii) its maintenance were analyzed. The rolling-circle plasmid pMV158, which replicates via ssDNA intermediates, contains two single-stra...

journal_title：Plasmid

authors： Meijer WJ,van der Lelie D,Venema G,Bron S

更新日期：1995-03-01 00:00:00

abstract：:The CoIV plasmids are large virulence plasmids of the incompatibility group IncFI. We have obtained the genetic maps of two of the most studied CoIV plasmids, pCoIV-B188 and pCoIV-K30, according to restriction enzyme sites and landmark phenotypic characteristics such as colicin V, the aerobactin iron uptake system, th...

journal_title：Plasmid

authors： Waters VL,Perez-Casal JF,Crosa JH

更新日期：1989-11-01 00:00:00

abstract：:Small particles of metallic tungsten, known also as tungsten microprojectiles, are routinely used for biotechnological purposes. In such applications, tungsten was observed to affect the integrity of plasmid DNA. Here we present evidence that interaction between tungsten particles and intact circular plasmids pU19, pU...

journal_title：Plasmid

authors： Mazuś B,Krysiak C,Buchowicz J

更新日期：2000-07-01 00:00:00

abstract：:The rapidly advancing field of plant synthetic biology requires transforming plants with multiple genes. This has sparked a growing interest in flexible plant transformation vectors, which can be used for multi-gene transformations. We have developed a novel binary vector series, named the PC-GW series (GenBank: KP826...

journal_title：Plasmid

authors： Dalal J,Yalamanchili R,La Hovary C,Ji M,Rodriguez-Welsh M,Aslett D,Ganapathy S,Grunden A,Sederoff H,Qu R

更新日期：2015-09-01 00:00:00

abstract：:Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons. Amplification products were obtained from 20 of the hybridization-positive strains. A single dfrA7 cassette was identified in 18 of the isolates and an integron with two cassettes (aadB-aadA4) ...

journal_title：Plasmid

authors： Segal H,Thomas R,Gay Elisha B

更新日期：2003-03-01 00:00:00

abstract：:The Gram-positive Corynebacterium glutamicum is widely known for its application in the industrial production of amino acids and as a non-pathogenic model organism for cell wall biosynthesis in the group of CMN bacteria. For biotechnological and physiological studies often co-expression of recombinant genes is require...

journal_title：Plasmid

authors： Goldbeck O,Seibold GM

更新日期：2018-01-01 00:00:00

abstract：:pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...

journal_title：Plasmid

authors： Chiang CS,Bremer H

更新日期：1991-11-01 00:00:00

abstract：:Two improved Deinococcus radiodurans-Escherichia coli shuttle vectors have been constructed. pI3 is a 16-kb plasmid that confers chloramphenicol resistance in D. radiodurans (CmR, cat) and ampicillin resistance in E. coli (ApR) and contains a multiple cloning site that does not interrupt sequences necessary for replic...

journal_title：Plasmid

authors： Masters CI,Minton KW

更新日期：1992-11-01 00:00:00

abstract：:Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...

journal_title：Plasmid

authors： D'Haeze W,Verplancke C,Mironov V,Holsters M

更新日期：2002-03-01 00:00:00

abstract：:The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that th...

journal_title：Plasmid

authors： Guay GG,Khan SA,Rothstein DM

更新日期：1993-09-01 00:00:00

abstract：:A chimeric plasmid, pRS7Rep (6.1 kb), was constructed using the replication region of pRS7, a large plasmid from Oenococcus oeni, and pEM64, a plasmid derived from pIJ2925 and containing a gene for resistance to chloramphenicol. pRS7Rep is a shuttle vector that replicates in Escherichia coli using its pIJ2925 componen...

journal_title：Plasmid

authors： Rodríguez MC,Alegre MT,Martín MC,Mesas JM

更新日期：2015-01-01 00:00:00