Abstract：
:Most Acinetobacter plasmids are genus specific but their properties have not been investigated. Small plasmids with Rep_3 family replication initiation proteins and iterons are common in Acinetobacter baumannii and often carry antibiotic resistance genes and toxin-antitoxin systems. A RepAci1 plasmid, carrying the carbapenem resistance gene oxa23 in Tn2006 and a RepAci2 plasmid carrying the amikacin (kanamycin and neomycin) resistance gene aphA6 in TnaphA6 were identified. These two plasmids have related rep regions; the consensus 22 bp iteron repeats differ only at three positions and the RepA proteins are 84% identical. However, they were shown to be compatible, whereas the RepAci1 plasmid displaced another RepAci1 plasmid demonstrating that they were incompatible. Despite encoding no mobilisation proteins, the RepAci1 plasmid was transferred to a new host at low frequency when a conjugatively proficient RepAci6 plasmid was present, whereas the RepAci2 plasmid carrying mobA and mobC mobilisation genes was not. Comparison of the sequences of the mobilised and mobilising plasmids revealed a short region of high similarity that is upstream of the predicted mobilisation genes in the RepAci6 plasmid, and has an organisation similar to that of F-type oriT transfer origins. The segment carrying the oriT-like region is present in many RepAci1 plasmids, including ones carrying the cabarpenem resistance genes oxa24 or oxa58 in dif modules, and in some RepAci2 or other Rep_3 plasmids of further types, including one carrying the tet39 tetracycline resistance determinant. These plasmids are also likely to be mobilised, spreading resistance.
journal_name
Plasmidjournal_title
Plasmidauthors
Blackwell GA,Hall RMdoi
10.1016/j.plasmid.2019.04.002subject
Has Abstractpub_date
2019-05-01 00:00:00pages
36-44eissn
0147-619Xissn
1095-9890pii
S0147-619X(19)30031-9journal_volume
103pub_type
杂志文章相关文献
PLASMID文献大全abstract：:Two distinct families of repetitive DNA elements (1.4 and 1.2 kb) were identified from S1 nuclease-treated genomic DNA of four strains of Thiobacillus ferrooxidans. The 1.4-kb fragment hybridized with IST2, an insertion sequence of T. ferrooxidans. The 1.2-kb fragment was cloned and sequenced. The sequence (IST445), 1...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1997.1306
更新日期：1997-01-01 00:00:00
abstract：:An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was developed. The method relies on two integration-proficient vectors, mini-CTX1 and mini-CTX2. These two vectors contain (1) a tetracycline (tet) selectable marker, (2) an oriT for conjugation-...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1999.1441
更新日期：2000-01-01 00:00:00
abstract：:Plasmid pT181 is a small multicopy plasmid from Staphylococcus aureus that belongs to incompatibility group 3 and expresses two distinct types of incompatibility, Inc3A and Inc3B. Inc3A incompatibility is expressed by the primary replication control determinant, copA, which specifies two small transcripts, RNA I and R...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(90)90039-f
更新日期：1990-01-01 00:00:00
abstract：:The incompatibility region of the Streptomyces plasmid pSL1 was identified as a 240-bp segment, though some other function from the vector plasmid was also necessary. A 540-bp fragment including the 240-bp region was enough for full expression of incompatibility. Inserted mutation analysis led to a more detailed locat...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(87)90021-7
更新日期：1987-03-01 00:00:00
abstract：:Rolling circle-replicating plasmids constitute a vast family that is particularly abundant in, but not exclusive of, Gram-positive bacteria. These plasmids are constructed as cassettes that harbor genes involved in replication and its control, mobilization, resistance determinants and one or two origins of lagging str...
journal_title：Plasmid
pub_type： 杂志文章,评审
doi：10.1016/j.plasmid.2014.05.004
更新日期：2014-07-01 00:00:00
abstract：:We have examined the extent to which the degradative plasmids SAL, NAH, and TOL of the Inc P-9 incompatibility group share common DNA sequences. The homology we observe using 32P-labeled SAL and NAH DNA probes can be assigned to six regions of the TOL (pWWO) restriction endonuclease cleavage map. At least three of the...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(83)90069-0
更新日期：1983-09-01 00:00:00
abstract：:The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of co...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(87)90009-6
更新日期：1987-01-01 00:00:00
abstract：:The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/s0147-619x(03)00059-3
更新日期：2003-09-01 00:00:00
abstract：:The CoIV plasmids are large virulence plasmids of the incompatibility group IncFI. We have obtained the genetic maps of two of the most studied CoIV plasmids, pCoIV-B188 and pCoIV-K30, according to restriction enzyme sites and landmark phenotypic characteristics such as colicin V, the aerobactin iron uptake system, th...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(89)90007-3
更新日期：1989-11-01 00:00:00
abstract：:Bacterial chromosomes segregate via a partition apparatus that employs a score of specialized proteins. The SMC complexes play a crucial role in the chromosome partitioning process by organizing bacterial chromosomes through their ATP-dependent chromatin-compacting activity. Recent progress in the composition of these...
journal_title：Plasmid
pub_type： 杂志文章,评审
doi：10.1016/j.plasmid.2005.08.004
更新日期：2006-03-01 00:00:00
abstract：:Naturally occurring plasmids have medical importance given that they frequently code for virulence or antibiotic resistance. In many cases, plasmids impose a fitness cost to their hosts, meaning that the growth rate of plasmid-bearing cells is lower than that of plasmid-free cells. However, this does not fit with the ...
journal_title：Plasmid
pub_type： 杂志文章,评审
doi：10.1016/j.plasmid.2018.09.009
更新日期：2018-09-01 00:00:00
abstract：:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1994.1062
更新日期：1994-09-01 00:00:00
abstract：:A number of plasmid vectors were constructed for high-level gene expression in the dimorphic gram-negative bacterium Caulobacter crescentus. These vectors incorporate the transcription and translation initiation regions of the C. crescentus CB15A rsaA gene, which codes for the abundantly synthesized protein comprising...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(90)90016-6
更新日期：1990-09-01 00:00:00
abstract：:Fusobacterium nucleatum is a Gram-negative anaerobic rod found in dental plaque biofilms, and is an opportunistic pathogen implicated in periodontitis as well as a wide range of systemic abscesses and infections. Genomic analyses of F. nucleatum indicate considerable genetic diversity and a prominent role for horizont...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2009.09.001
更新日期：2010-01-01 00:00:00
abstract：:Small particles of metallic tungsten, known also as tungsten microprojectiles, are routinely used for biotechnological purposes. In such applications, tungsten was observed to affect the integrity of plasmid DNA. Here we present evidence that interaction between tungsten particles and intact circular plasmids pU19, pU...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.2000.1475
更新日期：2000-07-01 00:00:00
abstract：:Bacterial plasmids and phages encode the synthesis of toxic molecules that inhibit protozoan predation. One such toxic molecule is violacein, a purple pigmented, anti-tumour antibiotic produced by the Gram-negative soil bacterium Chromobacterium violaceum. In the current experiments a range of Escherichia coli K12 str...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2011.07.006
更新日期：2011-09-01 00:00:00
abstract：:Sphingomonas sp. A1 (strain A1) is capable of directly incorporating macromolecules (e.g., alginate) through the specialized import system--"super-channel." Here, we report the complete DNA sequence and genetic organization of plasmid pA1 from strain A1. Nucleotide sequence analysis revealed that pA1 comprises 46,557 ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2005.12.002
更新日期：2006-07-01 00:00:00
abstract：:Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.2002.1565
更新日期：2002-03-01 00:00:00
abstract：:The large, 350-kb Sym (symbiotic) plasmid pRjaUSDA193 of Rhizobium fredii was examined to determine the frequency of repeated sequences present and to produce a physical and genetic map of a large region of the plasmid. A novel hybridization method, the Southern Cross, revealed that the plasmid pRjaUSDA193 contained m...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(86)90077-6
更新日期：1986-07-01 00:00:00
abstract：:A new cryptic plasmid from a multi-resistant, multi-plasmid clinical strain of Escherichia coli has been isolated. The sequence of the 4072-base-pair pIGWZ12 (GenBank Accession No. DQ311641) was determined and analyzed. Two open-reading frames that code for proteins involved in plasmid mobilization and initiation of r...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2006.05.004
更新日期：2006-11-01 00:00:00
abstract：:CspA has been identified as a major cold-shock protein in Escherichia coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatabi...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2015.06.002
更新日期：2015-09-01 00:00:00
abstract：:As multidrug resistant bacteria pose one of the greatest risks to human health new alternative antibacterial agents are urgently needed. One possible mechanism that can be used as an alternative to traditional antibiotic therapy is transfer of killing agents via conjugation. Our work was aimed at providing a proof of ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2015.10.001
更新日期：2015-11-01 00:00:00
abstract：:The joining of DNA ends was compared in the established CV1 cell line and in African green monkey kidney primary cells, from which the CV1 cell line was established. Using a linear SV40 genome that carries a terminal repeat of 526 nucleotide pairs, we measured the efficiency of circularization by end joining relative ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(89)90019-x
更新日期：1989-09-01 00:00:00
abstract：:Most Vibrio fischeri strains isolated from the Euprymna scolopes light organ carry plasmids, often including both a large (>40kb) plasmid, and one or more small (<12kb) plasmids. The large plasmids share homology with pES100, which is the lone plasmid in V. fischeri type strain ES114. pES100 appears to encode a conjug...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2005.01.003
更新日期：2005-09-01 00:00:00
abstract：UNLABELLED:This work describes a novel plasmid pSP187 (5550 bp) carrying the small multidrug resistance determinant smr encoding resistance to quaternary ammonium compounds (QACs). pSP187 was identified in a Staphylococcus pasteuri isolate recovered from bulk milk in a dairy cattle herd in Norway. Sequence analysis rev...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2006.08.004
更新日期：2007-03-01 00:00:00
abstract：:The imidazole and triazole fungicides inhibit cytochrome P450 14 alpha-lanosterol demethylase (P45014DM) implicated in the ergosterol biosynthesis pathway, which is specific to fungi and yeasts. Two plasmids were obtained which allow triazole and imidazole resistance in Saccharomyces cerevisiae. The low copy number pl...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1993.1054
更新日期：1993-11-01 00:00:00
abstract：:The rapidly advancing field of plant synthetic biology requires transforming plants with multiple genes. This has sparked a growing interest in flexible plant transformation vectors, which can be used for multi-gene transformations. We have developed a novel binary vector series, named the PC-GW series (GenBank: KP826...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2015.06.003
更新日期：2015-09-01 00:00:00
abstract：:Two B/O plasmids were sequenced. The kanamycin resistance plasmid R805a was found in a Salmonella Typhi strain from a 1972 typhoid fever outbreak in Mexico City. pCERC6, which confers resistance to ampicillin, streptomycin and sulphamethoxazole, was found in a commensal E. coli isolated from a healthy adult in Sydney ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2019.02.005
更新日期：2019-03-01 00:00:00
abstract：:The effects of mutations have been studied within the apocytochrome b gene on the processing of transcripts from the gene for subunit 1 of cytochrome c oxidase (coxI). Most mutations which affect the expression of the reading frame encoded by the fourth intron of the apocytochrome b gene (bI4) result in a failure to r...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(84)90065-9
更新日期：1984-07-01 00:00:00
abstract：:Nucleotide sequence analysis of the Tn4451-deletion derivatives, pJIR47 and pJIR86, which were derived from Escherichia coli and Clostridium perfringens, respectively, showed that the deletion events that led to the formation of these plasmids were identical and precise. The results also showed that the termini of thi...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(88)90055-8
更新日期：1988-03-01 00:00:00
