Abstract:
:A new cryptic plasmid from a multi-resistant, multi-plasmid clinical strain of Escherichia coli has been isolated. The sequence of the 4072-base-pair pIGWZ12 (GenBank Accession No. DQ311641) was determined and analyzed. Two open-reading frames that code for proteins involved in plasmid mobilization and initiation of replication were identified. The putative origin of replication possesses all characteristic features of the theta mechanism for replicating plasmids. pIGWZ12 is stably maintained without selective pressure in bacterial cultures (for up to 80 generations), making it a good candidate for engineering a new cloning vector.
journal_name
Plasmidjournal_title
Plasmidauthors
Zaleski P,Wolinowska R,Strzezek K,Lakomy A,Plucienniczak Adoi
10.1016/j.plasmid.2006.05.004subject
Has Abstractpub_date
2006-11-01 00:00:00pages
228-32issue
3eissn
0147-619Xissn
1095-9890pii
S0147-619X(06)00054-0journal_volume
56pub_type
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