Abstract:
:Sequence analysis of the Pseudomonas aeruginosa insertion sequence element IS222 revealed it to be 1234 bp in size with 23 bp imperfect terminal inverted repeats. Insertion caused a 5-bp duplication of the insertion site. Two ORFs were identified, one of which, ORFA, could encode a basic (pI 10.5) polypeptide with a mass of 11,709. This sequence bears strong homology to the putative ORFA product from the Shigella dysenteriae insertion sequence element IS911, which is a member of the IS3 family of insertion elements. As with other members of this group the nucleotide sequence contains a "frameshift window" (AAAAAAG; M. Chandler and O. Fayet (1993). Mol. Microbiol. 7, 497-503) at which ribosome slippage can result in a fusion protein (ORFAB).
journal_name
Plasmidjournal_title
Plasmidauthors
Kropinski AM,Farinha MA,Jansons Idoi
10.1006/plas.1994.1024subject
Has Abstractpub_date
1994-03-01 00:00:00pages
222-8issue
2eissn
0147-619Xissn
1095-9890pii
S0147-619X(84)71024-9journal_volume
31pub_type
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