Sequence analysis of two cryptic plasmids from an agricultural isolate of Campylobacter coli.

abstract：:A collection of 111 commensal Escherichia coli isolated from 84 faecal samples from healthy Australian adults were screened using PCR-based replicon typing. Each isolate represented a distinct strain found in a particular faecal sample. Fifty-one isolates were resistant to one or more of 12 antibiotics tested. FII and...

journal_title：Plasmid

authors： Moran RA,Anantham S,Pinyon JL,Hall RM

更新日期：2015-07-01 00:00:00

abstract：:A transient expression system has been developed to compare the relative efficiency of expression of various vaccinia virus DNA sequences containing transcriptional regulatory elements. A plasmid vector was constructed containing both the Escherichia coli galactokinase gene (galK) and the guanine phosphoribosyltransfe...

journal_title：Plasmid

authors： Shepard B,Panicali D,Huang C

更新日期：1987-07-01 00:00:00

abstract：:A small cryptic plasmid designated pHW15 was isolated from Rahnella genomospecies 2 WMR15 and its complete nucleotide sequence was determined. The plasmid contained 3002 bp with a G+C content of 47.4%. The origin of replication was identified by deletion analysis as a region of about 600 bp. This region had an identit...

journal_title：Plasmid

authors： Rozhon WM,Petutschnig EK,Jonak C

更新日期：2006-11-01 00:00:00

abstract：:Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...

journal_title：Plasmid

authors： Feng X,Ou LT,Ogram A

更新日期：1997-01-01 00:00:00

abstract：:We report the construction of two filamentous fungi Trichoderma reesei expression vectors, pWEF31 and pWEF32. Both vectors possess the hygromycin phosphotransferase B gene expression cassette and the strong promoter and terminator of the cellobiohydrolase 1 gene (cbh1) from T. reesei. The two newly constructed vectors...

journal_title：Plasmid

authors： Lv D,Wang W,Wei D

更新日期：2012-01-01 00:00:00

abstract：:We present the complete nucleotide sequence of a mer operon located on a 60-kb conjugative plasmid pKLH2 from an environmental bacterium, Acinetobacter calcoaceticus, isolated from a mercury mine. The pKLH2 mer operon has essentially the same gene organization as that of Tn21 and Tn501 from clinical bacteria. The pKLH...

journal_title：Plasmid

authors： Kholodii GY,Gorlenko Z,Lomovskaya OL,Mindlin SZ,Yurieva OV,Nikiforov VG

更新日期：1993-11-01 00:00:00

abstract：:Nucleotide sequence analysis of two cryptic plasmids, pRS2 (2544 bp) and pRS3 (3948 bp), from Oenococcus oeni revealed the presence in both of three major open reading frames with significant similarity to other small cryptic plasmids from O. oeni. The results suggest that those plasmids could be separated into two su...

journal_title：Plasmid

authors： Mesas JM,Rodríguez MC,Alegre MT

更新日期：2001-09-01 00:00:00

abstract：:Mutations that increase the copy number of the pSC101 replicon have been used for construction of new cloning vectors. Replacement of glutamate at position 93 in RepA yields plasmids that replicate at medium (27 copies/cell) and high (approximately 240 copies/cell) copy numbers. Based on the crystal structure of RepE,...

journal_title：Plasmid

authors： Peterson J,Phillips GJ

更新日期：2008-05-01 00:00:00

abstract：:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...

journal_title：Plasmid

authors： Yamamoto T,Hanawa T,Murayama SY,Ogata S

更新日期：1994-09-01 00:00:00

abstract：:Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence ...

journal_title：Plasmid

authors： Tabata A,Deutsch D,Otsuka S,Verratti K,Tomoyasu T,Nagamune H,Fischetti VA

更新日期：2018-01-01 00:00:00

abstract：:A 102-kb plasmid, pIJB1, was isolated from Burkholderia cepacia strain 2a, which is able to use 2,4-dichlorophenoxyacetate (2,4-D) as a sole carbon source, and a physical map of the plasmid has been established. It was observed that spontaneous loss of a 37-kb fragment of the plasmid after growth in nonselective mediu...

journal_title：Plasmid

authors： Xia XS,Aathithan S,Oswiecimska K,Smith AR,Bruce IJ

更新日期：1998-01-01 00:00:00

abstract：:Strain LBG A3136 of Nocardia mediterranei (ETH Collection) was found to contain a low-copy-number covalently closed circular extrachromosomal element, pMEA 100, which could only be isolated from mycelium grown on agar plates. pMEA 100 could not be isolated from the closely related strain ATCC 13685. Hybridization expe...

journal_title：Plasmid

authors： Moretti P,Hintermann G,Hütter R

更新日期：1985-09-01 00:00:00

abstract：:We report the mobilization by cointegration of the gonococcal 5.2 kb beta-lactamase plasmid pSJ5.2 in an Escherichia coli background. Transfer of pSJ5.2 was measured by filter mating assays with five different conjugative plasmids from Enterobacteriaceae and the gonococcal 41 kb tet(M). Plasmid pSJ5.2 was mobilized to...

journal_title：Plasmid

authors： Scharbaai-Vázquez R,Candelas T,Torres-Bauzá LJ

更新日期：2007-03-01 00:00:00

abstract：:The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of co...

journal_title：Plasmid

authors： Caillaud F,Carlier C,Courvalin P

更新日期：1987-01-01 00:00:00

abstract：:The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155....

journal_title：Plasmid

authors： Moran RA,Anantham S,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:Nucleotide sequence analysis of the Tn4451-deletion derivatives, pJIR47 and pJIR86, which were derived from Escherichia coli and Clostridium perfringens, respectively, showed that the deletion events that led to the formation of these plasmids were identical and precise. The results also showed that the termini of thi...

journal_title：Plasmid

authors： Abraham LJ,Rood JI

更新日期：1988-03-01 00:00:00

abstract：:The genus Bacteroides are gram-negative, obligate anaerobes indigenous to the gastrointestinal tract of humans and animals. The Bacteroides and other members of the Bacteroidetes phylum have diverged from the Proteobacteria. These organisms evolved a unique promoter structure for the initiation of transcription, hence...

journal_title：Plasmid

authors： Parker AC,Jeffrey Smith C

更新日期：2012-09-01 00:00:00

abstract：:pSM19035 is a low-copy-number theta-replicating plasmid, which belongs to the Inc18 family. Plasmids of this family, which show a modular organization, are functional in evolutionarily diverse bacterial species of the Firmicutes Phylum. This review summarizes our understanding, accumulated during the last 20 years, on...

journal_title：Plasmid

authors： Lioy VS,Pratto F,de la Hoz AB,Ayora S,Alonso JC

更新日期：2010-07-01 00:00:00

abstract：:A simple and reliable method for the determination of plasmid copy numbers by direct fluorescence densitometry of ethidium bromide-stained electrophoretic gels was developed. In developing the method, the following parameters were evaluated and controlled: plasmid DNA trapping in the linear chromosomal DNA, staining-d...

journal_title：Plasmid

authors： Projan SJ,Carleton S,Novick RP

更新日期：1983-03-01 00:00:00

abstract：:Mobile plasmid-encoded elements are DNA segments that are transferred for horizontal gene transfer and that confer adaptive proprieties, as well as virulence and antibiotic and heavy metal resistance to bacteria. The conjugative plasmid pUM505, isolated from a clinical strain of Pseudomonas aeruginosa, possesses a put...

journal_title：Plasmid

authors： Hernández-Ramírez KC,Reyes-Gallegos RI,Chávez-Jacobo VM,Díaz-Magaña A,Meza-Carmen V,Ramírez-Díaz MI

更新日期：2018-06-01 00:00:00

abstract：:Transposon-insertion mutants with vir- Ti plasmids were characterized and then used in complementation experiments. One of the mutants (LBA 1517) had a mutation in a newly discovered vir locus called virF. The virF mutation led to a strongly diminished virulence on tomato and tobacco, but not on certain other plant sp...

journal_title：Plasmid

authors： Hooykaas PJ,Hofker M,den Dulk-Ras H,Schilperoort RA

更新日期：1984-05-01 00:00:00

abstract：:The structure of several T-DNAs of Agrobacterium tumefaciens was determined by molecular cloning and Southern hybridization. The T-DNAs cloned in Escherichia coli vectors from four different nopaline type strains (PyTE1, PO31, PO22, and AKE10) showed various sizes of restriction enzyme fragments. Comparative analysis ...

journal_title：Plasmid

authors： Wabiko H,Kagaya M,Sano H

更新日期：1991-01-01 00:00:00

abstract：:Most Acinetobacter plasmids are genus specific but their properties have not been investigated. Small plasmids with Rep_3 family replication initiation proteins and iterons are common in Acinetobacter baumannii and often carry antibiotic resistance genes and toxin-antitoxin systems. A RepAci1 plasmid, carrying the car...

journal_title：Plasmid

authors： Blackwell GA,Hall RM

更新日期：2019-05-01 00:00:00

abstract：:The CoIV plasmids are large virulence plasmids of the incompatibility group IncFI. We have obtained the genetic maps of two of the most studied CoIV plasmids, pCoIV-B188 and pCoIV-K30, according to restriction enzyme sites and landmark phenotypic characteristics such as colicin V, the aerobactin iron uptake system, th...

journal_title：Plasmid

authors： Waters VL,Perez-Casal JF,Crosa JH

更新日期：1989-11-01 00:00:00

abstract：:An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was developed. The method relies on two integration-proficient vectors, mini-CTX1 and mini-CTX2. These two vectors contain (1) a tetracycline (tet) selectable marker, (2) an oriT for conjugation-...

journal_title：Plasmid

authors： Hoang TT,Kutchma AJ,Becher A,Schweizer HP

更新日期：2000-01-01 00:00:00

abstract：:Four plasmids encoding restriction and modification (R/M) systems are described that are different in the specificity of their restrictive activity toward the small isometric phage p2 and prolate phage c2. The R/M plasmids were cotransformed into Lactococcus lactis MG1363 with pVS2, encoding resistance to chlorampheni...

journal_title：Plasmid

authors： Josephsen J,Klaenhammer T

更新日期：1990-01-01 00:00:00

abstract：:RNA interference is a widely used tool for analysis of gene function in mammalian cells. Stable knockdown of specific target genes can be maintained in cell lines and live organisms using vector-based delivery of short hairpins (shRNAs) driven by RNA polymerase III promoters. Here we describe a vector incorporating th...

journal_title：Plasmid

authors： Sotirova VN,Calciano MA,Krueger W,Lalande M

更新日期：2006-05-01 00:00:00

abstract：:Variovorax sp. strain DB1 and Pseudomonas pickettii strain 712 are 2,4-dicholorophenoxy-acetic acid (2,4-D)-degrading bacteria, which were isolated from agricultural soils in Republic of Korea and USA, respectively. Each strain harbors a 2,4-D degradative plasmid and is able to utilize 2,4-D as the sole source of carb...

journal_title：Plasmid

authors： Kim DU,Kim MS,Lim JS,Ka JO

更新日期：2013-05-01 00:00:00

abstract：:A variant of the cryptic plasmid of Neisseria gonorrhoeae, 4.4 kb in size, was isolated and characterized at the molecular level. This variant harbored a 156-bp insertion which was located between coordinates 3134 and 3135 within the putative cppB gene using the 4.2-kb cryptic plasmid, pJD1, as a reference. The insert...

journal_title：Plasmid

authors： Roy RN,Bigelow N,Dillon JA

更新日期：1988-01-01 00:00:00

abstract：:Pinene is a monoterpene with wide industrial applications, especially as a promising high energy-density jet fuel. Traditional production of pinene on an industrial scale is material consumptive and has a low yield. As an alternative, microbial organisms have been engineered though advanced synthetic biological techni...

journal_title：Plasmid

authors： Bao SH,Zhang DY,Meng E

更新日期：2019-09-01 00:00:00