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Determination of plasmid copy number by fluorescence densitometry.

Abstract：

:A simple and reliable method for the determination of plasmid copy numbers by direct fluorescence densitometry of ethidium bromide-stained electrophoretic gels was developed. In developing the method, the following parameters were evaluated and controlled: plasmid DNA trapping in the linear chromosomal DNA, staining-destaining kinetics for ethidium bromide, linearity of the fluorescence response, and the effect of the molecular topology of DNA on ethidium bromide binding to DNA in agarose.

journal_name

Plasmid

journal_title

Plasmid

authors

Projan SJ,Carleton S,Novick RP

doi

10.1016/0147-619x(83)90019-7

subject

Has Abstract

pub_date

1983-03-01 00:00:00

pages

182-90

issue

2

eissn

0147-619X

issn

1095-9890

pii

0147-619X(83)90019-7

journal_volume

9

pub_type

杂志文章

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