Abstract:
:Escherichia coli pcnB gene was discovered as a regulator of ColE1 plasmid copy number about 20 years ago. Further studies indicated that this gene codes for poly(A) polymerase I (PAP I). However, although importance of RNA polyadenylation has been demonstrated and biochemical properties of PAP I have been studied extensively, little is known about regulation of the pcnB gene transcription. Here, we demonstrate that there are three transcription start sites upstream of the pcnB coding sequence. Activities of at least two of these promoters were in inverse correlation to bacterial growth rates, which agrees with recently published results indicating increased abundance of PAP I and higher levels of RNA polyadenylation in slowly growing bacteria.
journal_name
Plasmidjournal_title
Plasmidauthors
Jasiecki J,Wegrzyn Gdoi
10.1016/j.plasmid.2005.10.002keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
169-72issue
2eissn
0147-619Xissn
1095-9890pii
S0147-619X(05)00099-5journal_volume
55pub_type
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