Abstract:
:The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of conjugation, after cloningoff Escherichia coli. Analysis of the element by restriction endonucleases, molecular cloning, electron microscopy of heteroduplexes, DNA hybridization, and sequencing allowed us to establish a physical map of Tn1545, localize the resistance genes, determine their direction of transcription, and compare them with other characterized resistance determinants, and show that Tn1545 is not flanked by large terminal repeated sequences in opposite orientation.
journal_name
Plasmidjournal_title
Plasmidauthors
Caillaud F,Carlier C,Courvalin Pdoi
10.1016/0147-619x(87)90009-6subject
Has Abstractpub_date
1987-01-01 00:00:00pages
58-60issue
1eissn
0147-619Xissn
1095-9890pii
0147-619X(87)90009-6journal_volume
17pub_type
杂志文章相关文献
PLASMID文献大全abstract::The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(03)00059-3
更新日期:2003-09-01 00:00:00
abstract::The mercury(II)-resistance transposons Tn502 and Tn512 were sequenced and shown to be members of the Tn5053 family. They are currently the sole representatives from the clinical setting and were obtained from geographically disparate Pseudomonas aeruginosa strains. The family is comprised of six novel transposons that...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.08.003
更新日期:2011-01-01 00:00:00
abstract::Avirulence gene D (avrD) in strain PT23 of Pseudomonas syringae pv. tomato (Pst) specifies the production of syringolides, which are elicitors of plant defense reactions. An 83-kb indigenous plasmid (pPT23B) that carries avrD has been mapped and characterized and a putative par region was identified. pPT23B contains a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1029
更新日期:1994-05-01 00:00:00
abstract::pSK41 is a prototypical 46-kb conjugative multiresistance plasmid of Staphylococcus aureus. The pSK41 replication initiation protein (Rep) is rate-limiting for plasmid replication, and its expression is negatively regulated by a small, non-coding antisense transcript, RNAI, that is complementary to the rep mRNA leader...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.01.002
更新日期:2015-03-01 00:00:00
abstract::A new insertion variant of IS231 family, designated ISBth4, was identified from Bacillus thuringiensis MEX312. ISBth4 is 2046bp in length and is delimited by two 17bp inverted repeats (IR) with one mismatch, flanked by two perfect 11bp direct repeats (DR). ISBth4 contains two open reading frames (ORFs), ORF1 and ORF2,...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.10.002
更新日期:2010-01-01 00:00:00
abstract::An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was developed. The method relies on two integration-proficient vectors, mini-CTX1 and mini-CTX2. These two vectors contain (1) a tetracycline (tet) selectable marker, (2) an oriT for conjugation-...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1441
更新日期:2000-01-01 00:00:00
abstract::A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90059-j
更新日期:1992-11-01 00:00:00
abstract::Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons. Amplification products were obtained from 20 of the hybridization-positive strains. A single dfrA7 cassette was identified in 18 of the isolates and an integron with two cassettes (aadB-aadA4) ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(03)00011-8
更新日期:2003-03-01 00:00:00
abstract::Suggestions are made for the estimation of plasmid loss rates in bacterial populations that multiply by binary fission. The plasmid loss rate is defined as the probability of a division of a plasmid-carrying individual giving birth to one plasmid-free and one plasmid-carrying daughter cell. The unit of time is consequ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0042
更新日期:1996-11-01 00:00:00
abstract::Here we present evidence that the cryptic 5.6-kb plasmid found in certain strains of Streptococcus mutans is not involved in mutacin production. This evidence comes from demonstrating similarities between a plasmid-less strain T8 and a group II plasmid strain UA96. Both produce what appears to be an identical mutacin ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(90)90013-3
更新日期:1990-09-01 00:00:00
abstract::Isolates of Rhodococcus equi from pneumonic foals possess an 85- or 90-kb virulence-associated plasmid. A prominent, thermoregulated surface antigen, VapA, encoded by these plasmids is thought to be important in virulence. A 135-kb fragment containing the origin of replication of R. equi strain 103 virulence-associate...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1311
更新日期:1997-01-01 00:00:00
abstract::Fusobacterium nucleatum is a Gram-negative anaerobic rod found in dental plaque biofilms, and is an opportunistic pathogen implicated in periodontitis as well as a wide range of systemic abscesses and infections. Genomic analyses of F. nucleatum indicate considerable genetic diversity and a prominent role for horizont...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.09.001
更新日期:2010-01-01 00:00:00
abstract::A set of low copy number plasmid vectors for mammalian gene expression has been constructed. These vectors are derived from the previously described bacterial low copy number expression vectors, pWSK29 and pWKS30, which are present at six to eight copies per cell. The new plasmids also have the following useful proper...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1334
更新日期:1998-01-01 00:00:00
abstract::Results of restriction endonuclease analysis and Southern blot hybridization suggest that the R-plasmids from Vibrio anguillarum strains isolated in Japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. Molecular cloning experiments allowed identif...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(84)90038-6
更新日期:1984-11-01 00:00:00
abstract::The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.1008
更新日期:1995-01-01 00:00:00
abstract::Nocardia brasiliensis, is a bacteria that lives as saprophyte in soil and causes a disease called actinomycetoma in both human and animals. Nocardia brasiliensis is an intracellular, facultative bacterium that replicates and survives within host macrophages. The mechanisms involved in the evasion of the microbicidal a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.09.005
更新日期:2011-01-01 00:00:00
abstract::We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb E...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1063
更新日期:1994-11-01 00:00:00
abstract::Nonviral gene delivery has gained a lot of interest as a promising approach for gene therapy. Despite intensive studies and much progress the outcome of nonviral vectors has remained significantly weaker than that of viral vectors. A weak transfection efficiency of nonviral gene transfection is still limiting their in...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.09.003
更新日期:2010-01-01 00:00:00
abstract::The nucleotide sequence of two novel plasmids isolated from the extreme thermophilic anaerobic bacterium Anaerocellum thermophilum DSM6725 (A. thermophilum), growing optimally at 70 degrees C, has been determined. pBAS2 was found to be a 3653 bp plasmid with a GC content of 43%, and the sequence revealed 10 open readi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.06.001
更新日期:2004-09-01 00:00:00
abstract::The complete nucleotide sequence of the cryptic plasmid pCC1 from Corynebacterium callunae (4109 bp) was determined. DNA sequence analysis revealed five open reading frames longer than 200 bp. One of the deduced polypeptides showed homology with the Rep proteins encoded by plasmids of the pIJ101/pJV1 family of plasmid...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2003.09.002
更新日期:2004-01-01 00:00:00
abstract::The complete nucleotide sequence of a plasmid, designated pRJF1, isolated from a rumen bacterium of the genus Butyrivibrio, has been determined. pRJF1 is a small plasmid (2631 bp) which shows the high AT content (64%) typical of plasmids from gram-positive organisms. Computer analysis of sequence data revealed two maj...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1993.1008
更新日期:1993-01-01 00:00:00
abstract::The promiscuous IncPα plasmids RK2 and R995 encode a broad-host-range partition system, whose essential components include the incC and korB genes and a DNA site (O(B)) to which the korB product binds. IncC2, the smaller of the two incC products, is sufficient for stabilization of R995ΔincC. It is a member of the type...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2012.04.003
更新日期:2012-09-01 00:00:00
abstract::Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2002.1565
更新日期:2002-03-01 00:00:00
abstract::The effects of mutations have been studied within the apocytochrome b gene on the processing of transcripts from the gene for subunit 1 of cytochrome c oxidase (coxI). Most mutations which affect the expression of the reading frame encoded by the fourth intron of the apocytochrome b gene (bI4) result in a failure to r...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(84)90065-9
更新日期:1984-07-01 00:00:00
abstract::A putative insertion sequence (IS1473) has been cloned and sequenced. The 1087-bp element was found between the moaA and the ndhA genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pAO1 plasmid of Arthrobacter nicotinovorans. It is flanked by an imperfect repeat of 33 bp and carries ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.1272
更新日期:1997-01-01 00:00:00
abstract::Strain LBG A3136 of Nocardia mediterranei (ETH Collection) was found to contain a low-copy-number covalently closed circular extrachromosomal element, pMEA 100, which could only be isolated from mycelium grown on agar plates. pMEA 100 could not be isolated from the closely related strain ATCC 13685. Hybridization expe...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(85)90072-1
更新日期:1985-09-01 00:00:00
abstract::Plasmid RK2 codes for two species of the replication initiator protein TrfA (33 and 44 kDa). Both polypeptides are strongly associated with membrane fractions of Escherichia coli host cells (W. Firshein and P. Kim, Mol. Microbiol. 23, 1-10, 1997). We investigated the role of a 12-amino-acid hydrophobic region (HR) in ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1467
更新日期:2000-05-01 00:00:00
abstract::Escherichia coli strain K12 BM14, carrying plasmid R751 (51.4 kb; Tpr, Tra+, IncP), was used as donor in matings with reference strains of the six Brucella nomenspecies, not known so far to harbor naturally occurring plasmids. R751 was easily transferred to and between Brucella spp., at frequencies ranging between 10(...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1993.1016
更新日期:1993-03-01 00:00:00
abstract::Mobile plasmid-encoded elements are DNA segments that are transferred for horizontal gene transfer and that confer adaptive proprieties, as well as virulence and antibiotic and heavy metal resistance to bacteria. The conjugative plasmid pUM505, isolated from a clinical strain of Pseudomonas aeruginosa, possesses a put...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2018.07.003
更新日期:2018-06-01 00:00:00
abstract::The large, 350-kb Sym (symbiotic) plasmid pRjaUSDA193 of Rhizobium fredii was examined to determine the frequency of repeated sequences present and to produce a physical and genetic map of a large region of the plasmid. A novel hybridization method, the Southern Cross, revealed that the plasmid pRjaUSDA193 contained m...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(86)90077-6
更新日期:1986-07-01 00:00:00