Abstract:
:Plasmid RK2 codes for two species of the replication initiator protein TrfA (33 and 44 kDa). Both polypeptides are strongly associated with membrane fractions of Escherichia coli host cells (W. Firshein and P. Kim, Mol. Microbiol. 23, 1-10, 1997). We investigated the role of a 12-amino-acid hydrophobic region (HR) in the membrane association of TrfA. Epitope-tagged polypeptide fragments of TrfA that contained HR were expressed and found to be associated with membrane fractions. Site-directed mutagenesis of trfA revealed that changes of specific amino acids in HR can affect both TrfA association with the membrane and its ability to support replication of an RK2 oriV plasmid in vivo. These results are consistent with the hypothesis that membrane association of TrfA is functionally relevant and that the HR region of TrfA is involved in membrane association and DNA replication in vivo.
journal_name
Plasmidjournal_title
Plasmidauthors
Kim PD,Rosche TM,Firshein Wdoi
10.1006/plas.2000.1467keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
214-22issue
3eissn
0147-619Xissn
1095-9890pii
S0147-619X(00)91467-7journal_volume
43pub_type
杂志文章相关文献
PLASMID文献大全abstract::A new plasmid, pNQ116, was constructed in Bacillus sphaericus by cloning a promoter fragment from B. sphaericus Ts-1 into pNQ112. The plasmid (CmrKmr, 5.23 kb) contains a restriction endonuclease polylinker used for cloning foreign genes, and its cat-86 gene is expressed at high levels from the Ts-1 promoter. This pla...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90026-7
更新日期:1992-05-01 00:00:00
abstract::The imidazole and triazole fungicides inhibit cytochrome P450 14 alpha-lanosterol demethylase (P45014DM) implicated in the ergosterol biosynthesis pathway, which is specific to fungi and yeasts. Two plasmids were obtained which allow triazole and imidazole resistance in Saccharomyces cerevisiae. The low copy number pl...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1993.1054
更新日期:1993-11-01 00:00:00
abstract::By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sens...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(83)90045-8
更新日期:1983-11-01 00:00:00
abstract::We describe the construction of a series of shuttle vectors for Staphylococcus aureus. In order to determine transcriptional regulation by essential regulators, we constructed promoterless luxABCDE reporter system using a TetR-regulated antisense RNA expression vector, pJYJ909, which is composed of S. aureus plasmid p...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.02.001
更新日期:2009-05-01 00:00:00
abstract::Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurring compatible members of the Dictyostelium Ddp1 plasmid family found in the same wild isolate strain. The nucleotide sequences of Dmp1 (5983 bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading frames (ORFs) similar to the G1 and G5...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1013
更新日期:1994-03-01 00:00:00
abstract::A suppressor of the mutations which inhibit the establishment of lysogeny in Pseudomonas aeruginosa has been found to be encoded by P. aeruginosa plasmids of four separate incompatibility groups. The wide distribution of this phenotype (designated Sly for suppressor of lysogeny establishment deficiency) indicates that...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(84)90024-6
更新日期:1984-03-01 00:00:00
abstract::A new class of binary vectors has been constructed, containing the origin of replication of the W-type plasmid Sa. These vectors are designed to express foreign genes in plants under control of the TR gene 2' promoter or the promoter of a light-inducible ribulose-1,5-bisphosphate carboxylase small subunit gene of Arab...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(88)90043-1
更新日期:1988-05-01 00:00:00
abstract::Upon excision, conjugative transposon Tn916 forms a nonreplicating circular intermediate (CI), which is essential both for transposition and conjugal transfer. In this work we developed an assay to quantify the circular forms of Tn916. By this method we defined a new measurable trait of Tn916-carrying strains: the "CI...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.1032
更新日期:1995-07-01 00:00:00
abstract::Recently the plasmid RK2 replication initiation protein, TrfA, has been shown to bind to the beta subunit of DNA Polymerase III (DnaN) via a short pentapeptide with the consensus QL[S/D]LF. A second consensus peptide, the hexapeptide QLxLxL, has also been demonstrated to mediate binding to DnaN. Here we describe the r...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2006.07.005
更新日期:2007-01-01 00:00:00
abstract::In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restric...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(90)90007-y
更新日期:1990-11-01 00:00:00
abstract::A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90059-j
更新日期:1992-11-01 00:00:00
abstract::The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of co...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(87)90009-6
更新日期:1987-01-01 00:00:00
abstract::The complete nucleotide sequence of the cryptic plasmid pCC1 from Corynebacterium callunae (4109 bp) was determined. DNA sequence analysis revealed five open reading frames longer than 200 bp. One of the deduced polypeptides showed homology with the Rep proteins encoded by plasmids of the pIJ101/pJV1 family of plasmid...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2003.09.002
更新日期:2004-01-01 00:00:00
abstract::During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1062
更新日期:1994-09-01 00:00:00
abstract::Pseudomonas putida is a highly attractive production system for industrial needs. However, for its improvement as a biocatalyst at the industrial level, modulation of its gene expression is urgently needed. We report the construction of a plasmid expressing a small RNA-based system with the potential to be used for di...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2020.102503
更新日期:2020-05-01 00:00:00
abstract::Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2002.1565
更新日期:2002-03-01 00:00:00
abstract::Unequal crossing-over is involved in genetic duplication and deletion in such diverse genetic systems as Drosophila, bacteria, and animal viruses. It is proposed to be involved in the form of unequal sister chromatid exchange in gene amplification in cultured animal cells and during carcinogenesis. Studies of the proc...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(85)90042-3
更新日期:1985-05-01 00:00:00
abstract::A flexible M13 vector library incorporating the BstXI site has been developed. DNA cut by any currently commercially available restriction endonuclease that generates a 4-nucleotide (nt) 3' overhang can be ligated into a specific clone of the library. The BstXI enzyme recognizes a 6-bp bipartite palindromic sequence. ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(90)90026-9
更新日期:1990-07-01 00:00:00
abstract::Bacterial plasmids and phages encode the synthesis of toxic molecules that inhibit protozoan predation. One such toxic molecule is violacein, a purple pigmented, anti-tumour antibiotic produced by the Gram-negative soil bacterium Chromobacterium violaceum. In the current experiments a range of Escherichia coli K12 str...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2011.07.006
更新日期:2011-09-01 00:00:00
abstract::Borrelia species comprise a unique genus of bacterial pathogens. These organisms contain a segmented genome with up to two dozen plasmids ranging in size from 5 kb up to about 200 kb. The plasmids have also been referred to as mini-chromosomes or essential genetic elements, as some of them carry information important ...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2013.05.002
更新日期:2013-09-01 00:00:00
abstract::pSK41 is a prototypical 46-kb conjugative multiresistance plasmid of Staphylococcus aureus. The pSK41 replication initiation protein (Rep) is rate-limiting for plasmid replication, and its expression is negatively regulated by a small, non-coding antisense transcript, RNAI, that is complementary to the rep mRNA leader...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.01.002
更新日期:2015-03-01 00:00:00
abstract::The ability of some bacteria to grow in the presence of high concentrations of tellurium compounds has been recognized for almost 100 years. Since then, interest in this phenomenon has generated a slow but steady trickle of literature. In the past few years, the use of modern techniques in molecular biology has led to...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/0147-619x(92)90006-v
更新日期:1992-01-01 00:00:00
abstract::Nocardia brasiliensis, is a bacteria that lives as saprophyte in soil and causes a disease called actinomycetoma in both human and animals. Nocardia brasiliensis is an intracellular, facultative bacterium that replicates and survives within host macrophages. The mechanisms involved in the evasion of the microbicidal a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.09.005
更新日期:2011-01-01 00:00:00
abstract::The chimeric plasmid pBN183 was first constructed in Escherichia coli by ligating the BamHI-digested E. coli plasmid pBR322 and a Bg/II-linearized streptococcal plasmid, pNZ18. The pBN183 transformed E. coli to ApR at a frequency of (8.2 +/- 1.2) x 10(5) colony forming units (CFU)/microgram DNA. Electrotransformation ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90033-7
更新日期:1992-07-01 00:00:00
abstract::Transformation of Escherichia coli with Dictyostelium discoideum genomic DNA containing integrated shuttle vectors in multicopy, tandemly duplicated format resulted in the establishment of the linear plasmid molecules as circular monomeric replicons. The transformation efficiencies were comparable to those obtained wi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0036
更新日期:1996-09-01 00:00:00
abstract::Even though Vibrio cholerae is a well-known human pathogen, it is also a normal member of aquatic habitats. Within this environment it often forms biofilms on the chitin-containing exoskeleton of crustaceans and their molts. Chitin not only serves as nutrient source but also induces a developmental program called natu...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.08.001
更新日期:2010-11-01 00:00:00
abstract::A combined physical and functional map of plasmid RP4 is presented including the sites for 18 restriction endonucleases. Several cleavage sites of SphI, BalI, and ApaI are suitable for the dissection of the transfer gene regions. Recombinant plasmids containing RP4 SphI fragments were constructed to assist in localizi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(83)90047-1
更新日期:1983-11-01 00:00:00
abstract::Small particles of metallic tungsten, known also as tungsten microprojectiles, are routinely used for biotechnological purposes. In such applications, tungsten was observed to affect the integrity of plasmid DNA. Here we present evidence that interaction between tungsten particles and intact circular plasmids pU19, pU...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1475
更新日期:2000-07-01 00:00:00
abstract::Little knowledge of DNA replication in cyanobacteria is available. In this study, we report the development and characterization of an in vitro system for studies of replication of the endogenous plasmids from the unicellular cyanobacterium Synechocystis 6803. This system (fraction III) was isolated at high salt conce...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1055
更新日期:1994-09-01 00:00:00
abstract::The large, 350-kb Sym (symbiotic) plasmid pRjaUSDA193 of Rhizobium fredii was examined to determine the frequency of repeated sequences present and to produce a physical and genetic map of a large region of the plasmid. A novel hybridization method, the Southern Cross, revealed that the plasmid pRjaUSDA193 contained m...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(86)90077-6
更新日期:1986-07-01 00:00:00