The presence of repeated DNA sequences and a partial restriction map of the pSym of Rhizobium fredii USDA193.

abstract：:We have constructed a pUC19-based multipurpose ATG vector in Schizosaccharomyces pombe with higher copy number and mitotic stability possible with commonly used vectors. The vector, having an NdeI site in its polylinker to provide ATG site for expression, carries a greatly truncated version of URA3 gene, URA3m, of Sac...

journal_title：Plasmid

authors： Verma HK,Singh J

更新日期：2012-11-01 00:00:00

abstract：:As multidrug resistant bacteria pose one of the greatest risks to human health new alternative antibacterial agents are urgently needed. One possible mechanism that can be used as an alternative to traditional antibiotic therapy is transfer of killing agents via conjugation. Our work was aimed at providing a proof of ...

journal_title：Plasmid

authors： Starčič Erjavec M,Petkovšek Ž,Kuznetsova MV,Maslennikova IL,Žgur-Bertok D

更新日期：2015-11-01 00:00:00

abstract：:Recently the plasmid RK2 replication initiation protein, TrfA, has been shown to bind to the beta subunit of DNA Polymerase III (DnaN) via a short pentapeptide with the consensus QL[S/D]LF. A second consensus peptide, the hexapeptide QLxLxL, has also been demonstrated to mediate binding to DnaN. Here we describe the r...

journal_title：Plasmid

authors： Dalrymple BP,Kongsuwan K,Wijffels G

更新日期：2007-01-01 00:00:00

abstract：:The plasmid pMSP3535 is a popular vector for nisin-inducible expression of heterologous genes in lactic acid bacteria. However, the maximum protein expression level achievable with pMSP3535 is relatively low. In an effort to increase expression we modified pMSP3535 to create a high expression variant termed pMSP3535H2...

journal_title：Plasmid

authors： Kim JH,Mills DA

更新日期：2007-11-01 00:00:00

abstract：:Results of restriction endonuclease analysis and Southern blot hybridization suggest that the R-plasmids from Vibrio anguillarum strains isolated in Japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. Molecular cloning experiments allowed identif...

journal_title：Plasmid

authors： Mitoma Y,Aoki T,Crosa JH

更新日期：1984-11-01 00:00:00

abstract：:The Tra1 region of RP1 from a derivative with Tn7 inserted into the kanamycin resistance determinant was cloned, using EcoRI, into the multicopy vector plasmid pBR325. For one orientation of the cloned fragment the resultant chimeric plasmid was very frequently lost from the cell, but in the other orientation it was m...

journal_title：Plasmid

authors： Watson J,Schmidt L,Willetts N

更新日期：1980-09-01 00:00:00

abstract：:In order to study the in vivo assembly of chromatin on prokaryotic DNA templates, we have transformed yeast cells with plasmids pAJ50 and pRB58, which contain pBR322 sequences. In both cases nucleosomes are assembled in vivo on pBR322 DNA, although the nucleosomes are not homogeneous in size. To explore whether there ...

journal_title：Plasmid

authors： Estruch F,Pérez-Ortín JE,Matallana E,Franco L

更新日期：1989-03-01 00:00:00

abstract：:We have examined the extent to which the degradative plasmids SAL, NAH, and TOL of the Inc P-9 incompatibility group share common DNA sequences. The homology we observe using 32P-labeled SAL and NAH DNA probes can be assigned to six regions of the TOL (pWWO) restriction endonuclease cleavage map. At least three of the...

journal_title：Plasmid

authors： Lehrbach PR,McGregor I,Ward JM,Broda P

更新日期：1983-09-01 00:00:00

abstract：:The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of co...

journal_title：Plasmid

authors： Caillaud F,Carlier C,Courvalin P

更新日期：1987-01-01 00:00:00

abstract：:Mud (mini-Mu) transposons are defective phage Mu genomes that conserve the Mu ends. The transduction of Mud transposons is strictly dependent on Mu complementation, inefficient, and affected by modifications in the Mud internal sequences. The transduction of Mud transposons depends on transposition, which appears to b...

journal_title：Plasmid

authors： Reyes O,Beyou A,Mignotte-Vieux C,Richaud F

更新日期：1987-11-01 00:00:00

abstract：:The complete sequence of the 10.9-kbp bacteriocinogenic plasmid pBL1 from Lactococcus lactis subsp. lactis IPLA 972 has been determined. Thirteen ORFs were encountered, of which 5 were incomplete. pBL1 proved to be a narrow-host-range plasmid which replicates neither in Bacilus subtilis nor in Lactobacillus spp. The s...

journal_title：Plasmid

authors： Sánchez C,Hernández de Rojas A,Martínez B,Argüelles ME,Suárez JE,Rodríguez A,Mayo B

更新日期：2000-11-01 00:00:00

abstract：:Homology was previously detected between the DNA restriction fragments containing Rhizobium meliloti nodulation genes and the 90-MDa plasmid, p90, of Azospirillum brasilense Sp7. Two DNA loci from Sp7 genome that complement mutations in the exopolysaccharide synthesis genes, exoB and exoC, of R. meliloti were also sho...

journal_title：Plasmid

authors： Onyeocha I,Vieille C,Zimmer W,Baca BE,Flores M,Palacios R,Elmerich C

更新日期：1990-05-01 00:00:00

abstract：:Two improved Deinococcus radiodurans-Escherichia coli shuttle vectors have been constructed. pI3 is a 16-kb plasmid that confers chloramphenicol resistance in D. radiodurans (CmR, cat) and ampicillin resistance in E. coli (ApR) and contains a multiple cloning site that does not interrupt sequences necessary for replic...

journal_title：Plasmid

authors： Masters CI,Minton KW

更新日期：1992-11-01 00:00:00

abstract：:We present the complete nucleotide sequence of a mer operon located on a 60-kb conjugative plasmid pKLH2 from an environmental bacterium, Acinetobacter calcoaceticus, isolated from a mercury mine. The pKLH2 mer operon has essentially the same gene organization as that of Tn21 and Tn501 from clinical bacteria. The pKLH...

journal_title：Plasmid

authors： Kholodii GY,Gorlenko Z,Lomovskaya OL,Mindlin SZ,Yurieva OV,Nikiforov VG

更新日期：1993-11-01 00:00:00

abstract：:We have modified an E. coli-staphylococcal shuttle vector for use in the general cloning and expression of genes from pathogenic staphylococci in Staphylococcus carnosus. As S. carnosus is non-pathogenic, this expression system will facilitate the study of the roles of individual gene products in the disease process. ...

journal_title：Plasmid

authors： Williams RJ,Nair SP,Henderson B,Holland KT,Ward JM

更新日期：2002-05-01 00:00:00

abstract：:Single plasmids designed to express the six human type I steroid hormone receptors and detect signaling activity are described in this report. These stably replicating plasmids reported ligand-induced transcriptional activation via lacZ assays in Baker's yeast (Saccharomyces cerevisiae). The ligand concentrations need...

journal_title：Plasmid

authors： Miller CA 3rd,Tan X,Wilson M,Bhattacharyya S,Ludwig S

更新日期：2010-03-01 00:00:00

abstract：:Two B/O plasmids were sequenced. The kanamycin resistance plasmid R805a was found in a Salmonella Typhi strain from a 1972 typhoid fever outbreak in Mexico City. pCERC6, which confers resistance to ampicillin, streptomycin and sulphamethoxazole, was found in a commensal E. coli isolated from a healthy adult in Sydney ...

journal_title：Plasmid

authors： Moran RA,Richardson IA,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:The genus Bacteroides are gram-negative, obligate anaerobes indigenous to the gastrointestinal tract of humans and animals. The Bacteroides and other members of the Bacteroidetes phylum have diverged from the Proteobacteria. These organisms evolved a unique promoter structure for the initiation of transcription, hence...

journal_title：Plasmid

authors： Parker AC,Jeffrey Smith C

更新日期：2012-09-01 00:00:00

abstract：:The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155....

journal_title：Plasmid

authors： Moran RA,Anantham S,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb E...

journal_title：Plasmid

authors： Kawaguchi R,Nagaoka T,Burgess JG,Takeyama H,Matsunaga T

更新日期：1994-11-01 00:00:00

abstract：:Nonviral gene delivery has gained a lot of interest as a promising approach for gene therapy. Despite intensive studies and much progress the outcome of nonviral vectors has remained significantly weaker than that of viral vectors. A weak transfection efficiency of nonviral gene transfection is still limiting their in...

journal_title：Plasmid

authors： Soininen P,Hanzlíková M,Paukkunen M,Lecklin A,Männistö PT,Raasmaja A

更新日期：2010-01-01 00:00:00

abstract：:The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that th...

journal_title：Plasmid

authors： Guay GG,Khan SA,Rothstein DM

更新日期：1993-09-01 00:00:00

abstract：:A chimeric plasmid, pRS7Rep (6.1 kb), was constructed using the replication region of pRS7, a large plasmid from Oenococcus oeni, and pEM64, a plasmid derived from pIJ2925 and containing a gene for resistance to chloramphenicol. pRS7Rep is a shuttle vector that replicates in Escherichia coli using its pIJ2925 componen...

journal_title：Plasmid

authors： Rodríguez MC,Alegre MT,Martín MC,Mesas JM

更新日期：2015-01-01 00:00:00

abstract：:The sequences of three transfer RNAs from mosquito cell mitochondria, tRNAArgUCG, tRNAAspGUC, and tRNAIleGAU, determined using a combination of rapid ladder and fingerprinting procedures are reported. These were compared with hamster mitochondrial tRNAArgUCG and tRNAAspGUC determined similarly, and a bovine mitochondr...

journal_title：Plasmid

authors： HsuChen CC,Cleaves GR,Dubin DT

更新日期：1983-07-01 00:00:00

abstract：:Here we present evidence that the cryptic 5.6-kb plasmid found in certain strains of Streptococcus mutans is not involved in mutacin production. This evidence comes from demonstrating similarities between a plasmid-less strain T8 and a group II plasmid strain UA96. Both produce what appears to be an identical mutacin ...

journal_title：Plasmid

authors： Caufield PW,Shah G,Hollingshead SK,Parrot M,Lavoie MC

更新日期：1990-09-01 00:00:00

abstract：:Transposon-insertion mutants with vir- Ti plasmids were characterized and then used in complementation experiments. One of the mutants (LBA 1517) had a mutation in a newly discovered vir locus called virF. The virF mutation led to a strongly diminished virulence on tomato and tobacco, but not on certain other plant sp...

journal_title：Plasmid

authors： Hooykaas PJ,Hofker M,den Dulk-Ras H,Schilperoort RA

更新日期：1984-05-01 00:00:00

abstract：:The structural and functional properties of mercury resistance determinants cloned from a series of independently isolated conjugative plasmids were compared with those of the prototype HgR determinants from Tn501 and plasmid R100 (containing Tn21). Restriction endonuclease mapping classified the HgR determinants into...

journal_title：Plasmid

authors： Jobling MG,Peters SE,Ritchie DA

更新日期：1988-09-01 00:00:00

abstract：:A 102-kb plasmid, pIJB1, was isolated from Burkholderia cepacia strain 2a, which is able to use 2,4-dichlorophenoxyacetate (2,4-D) as a sole carbon source, and a physical map of the plasmid has been established. It was observed that spontaneous loss of a 37-kb fragment of the plasmid after growth in nonselective mediu...

journal_title：Plasmid

authors： Xia XS,Aathithan S,Oswiecimska K,Smith AR,Bruce IJ

更新日期：1998-01-01 00:00:00

abstract：:Taking advantage of the plaque morphology method, we detected two single-stranded initiation (ssi) signals in the plasmid pSY343; one was in the 170-nucleotide (nt) EcoRV-ThaI segment (170P), and the other was in the 93-nt DraI-FnuDII segment (93F), which were designated as ssiA and ssiB, respectively. We cloned the t...

journal_title：Plasmid

authors： Bahk JD,Kioka N,Sakai H,Komano T

更新日期：1988-11-01 00:00:00

abstract：:Recently an insertional mutagenesis procedure has been developed to permit cloning of genes affected in developmental mutants of Dictyostelium discoideum (Kuspa and Loomis, Proc. Natl. Acad. Sci. USA 89, 8803-8807, 1992). In this procedure a plasmid bearing the URA (pyr5-6) gene is linearized with a restriction enzyme...

journal_title：Plasmid

authors： Chang WT,Gross JD,Newell PC

更新日期：1995-11-01 00:00:00