Mini-Mu transduction: cis-inhibition of the insertion of Mud transposons.

abstract：:Tn1935, a 23.5-kb transposon mediating resistance to ampicillin, kanamycin, mercury, spectinomycin, and sulfonamide was isolated from pZM3, an IncFIme virulence plasmid from Salmonella wien. Tn1935 possesses the entire sequence of Tn21 and contains two additional DNA segments of 0.95 and 2.7 kb carrying the ampicillin...

journal_title：Plasmid

authors： Colonna B,Bernardini M,Micheli G,Maimone F,Nicoletti M,Casalino M

更新日期：1988-11-01 00:00:00

abstract：:The joining of DNA ends was compared in the established CV1 cell line and in African green monkey kidney primary cells, from which the CV1 cell line was established. Using a linear SV40 genome that carries a terminal repeat of 526 nucleotide pairs, we measured the efficiency of circularization by end joining relative ...

journal_title：Plasmid

authors： Zheng H,Chang XB,Wilson JH

更新日期：1989-09-01 00:00:00

abstract：:Plasmid RK2 codes for two species of the replication initiator protein TrfA (33 and 44 kDa). Both polypeptides are strongly associated with membrane fractions of Escherichia coli host cells (W. Firshein and P. Kim, Mol. Microbiol. 23, 1-10, 1997). We investigated the role of a 12-amino-acid hydrophobic region (HR) in ...

journal_title：Plasmid

authors： Kim PD,Rosche TM,Firshein W

更新日期：2000-05-01 00:00:00

abstract：:Heterologous expression in Escherichia coli is often limited by yield and solubility of the foreign protein in the bacterial cytoplasm. In many cases, overexpression also results in growth inhibition. In order to produce retroviral proteins that are especially difficult to overexpress in E. coli, we designed a set of ...

journal_title：Plasmid

authors： Valverde V,Duplan H,Knibiehler M,Masson JM

更新日期：1994-07-01 00:00:00

abstract：:Brevibacterium linens RBL strain cryptic plasmid pRBL1 (8.0 kb) is described. A region involved in pRBL1 autonomous replication in Corynebacterium glutamicum was identified by insertion and deletion mapping and partially sequenced. This region encodes for a hypothetical 310-amino acid (aa) protein closely related to t...

journal_title：Plasmid

authors： Ankri S,Bouvier I,Reyes O,Predali F,Leblon G

更新日期：1996-07-01 00:00:00

abstract：:We have modified an E. coli-staphylococcal shuttle vector for use in the general cloning and expression of genes from pathogenic staphylococci in Staphylococcus carnosus. As S. carnosus is non-pathogenic, this expression system will facilitate the study of the roles of individual gene products in the disease process. ...

journal_title：Plasmid

authors： Williams RJ,Nair SP,Henderson B,Holland KT,Ward JM

更新日期：2002-05-01 00:00:00

abstract：:The two-hybrid system was used to show that the Rep proteins from three members of the Dictyostelium discoideum Ddp2 plasmid family, Ddp2, Ddp5, and Ddp6, form homomultimers but not heteromultimers when expressed in yeast cells. The results with deletion mutations suggest that multiple regions of the Rep proteins are ...

journal_title：Plasmid

authors： Shammat IM,Welker DL

更新日期：1999-05-01 00:00:00

abstract：:An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was developed. The method relies on two integration-proficient vectors, mini-CTX1 and mini-CTX2. These two vectors contain (1) a tetracycline (tet) selectable marker, (2) an oriT for conjugation-...

journal_title：Plasmid

authors： Hoang TT,Kutchma AJ,Becher A,Schweizer HP

更新日期：2000-01-01 00:00:00

abstract：:The construction of a set of new plasmids that are suitable as general cloning vectors in Escherichia coli and Agrobacterium tumefaciens is described. Plasmid pUCD2 is amplifiable in E. coli, replicates in a wide range of gram-negative hosts and contains a number of useful restriction endonuclear cleavage sites and an...

journal_title：Plasmid

authors： Close TJ,Zaitlin D,Kado CI

更新日期：1984-09-01 00:00:00

abstract：:Borrelia species comprise a unique genus of bacterial pathogens. These organisms contain a segmented genome with up to two dozen plasmids ranging in size from 5 kb up to about 200 kb. The plasmids have also been referred to as mini-chromosomes or essential genetic elements, as some of them carry information important ...

journal_title：Plasmid

authors： Chaconas G,Norris SJ

更新日期：2013-09-01 00:00:00

abstract：:Transcription of the 7.5-kb cryptic plasmid of Chlamydia trachomatis serovar L2 was investigated. Faint, diffuse transcripts of about 1.6 and 2.2 kb and intense short transcripts of about 250 and 430 bases were identified by Northern blot analysis. The short transcripts were found to have a common 5' end corresponding...

journal_title：Plasmid

authors： Fahr MJ,Sriprakash KS,Hatch TP

更新日期：1992-11-01 00:00:00

abstract：:CspA has been identified as a major cold-shock protein in Escherichia coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatabi...

journal_title：Plasmid

authors： Kang N,Choi E,Kim SG,Hwang J

更新日期：2015-09-01 00:00:00

abstract：:As multidrug resistant bacteria pose one of the greatest risks to human health new alternative antibacterial agents are urgently needed. One possible mechanism that can be used as an alternative to traditional antibiotic therapy is transfer of killing agents via conjugation. Our work was aimed at providing a proof of ...

journal_title：Plasmid

authors： Starčič Erjavec M,Petkovšek Ž,Kuznetsova MV,Maslennikova IL,Žgur-Bertok D

更新日期：2015-11-01 00:00:00

abstract：:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...

journal_title：Plasmid

authors： Yamamoto T,Hanawa T,Murayama SY,Ogata S

更新日期：1994-09-01 00:00:00

abstract：:This review provides a historical perspective of the major findings that contributed to our current understanding of plasmid rolling-circle (RC) replication. Rolling-circle-replicating (RCR) plasmids were discovered approximately 20 years ago. The first of the RCR plasmids to be identified were native to Gram-positive...

journal_title：Plasmid

authors： Khan SA

更新日期：2005-03-01 00:00:00

abstract：:A preliminary screening study of six Moraxella catarrhalis isolates from primary school children in the Netherlands identified a small 3.5 kb plasmid (pEMCJH03), containing four open reading frames, which encoded three mobilizing and one replicase protein. Insertion of a kanamycin containing transposon (yielding pEMCJ...

journal_title：Plasmid

authors： Hays JP,Eadie K,Verduin CM,Verbrugh H,van Belkum A

更新日期：2005-05-01 00:00:00

abstract：:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...

journal_title：Plasmid

authors： Taylor DE,Brose EC

更新日期：1985-01-01 00:00:00

abstract：:Plasmid deletion formation was compared in wild-type, recE4, and addB72 strains of Bacillus subtilis. Deletion frequencies in plasmid pGP1, as monitored with a penP-lacZ fusion, were low in recE4 and high in addB72, in comparison to the wild-type strain. In the wild-type and recE4 strains, deletions between directly r...

journal_title：Plasmid

authors： Peijnenburg AC,Breed PV,Bron S,Venema G

更新日期：1989-05-01 00:00:00

abstract：:We have constructed shuttle vectors for integration of genes via double homologous recombination into three ectopic sites on the chromosome of Bacillus subtilis. The sites of integration are the pyrD, gltA, and sacA genes located at 139 degrees, 172 degrees, and 333 degrees, respectively, on the chromosome. Integratio...

journal_title：Plasmid

authors： Middleton R,Hofmeister A

更新日期：2004-05-01 00:00:00

abstract：:A new plasmid, pNQ116, was constructed in Bacillus sphaericus by cloning a promoter fragment from B. sphaericus Ts-1 into pNQ112. The plasmid (CmrKmr, 5.23 kb) contains a restriction endonuclease polylinker used for cloning foreign genes, and its cat-86 gene is expressed at high levels from the Ts-1 promoter. This pla...

journal_title：Plasmid

authors： Qiao M,Gao C,Cai B,Zhang X,Wang S

更新日期：1992-05-01 00:00:00

abstract：:We report here the molecular characterization of pFNL10, a 3990-bp cryptic plasmid of Francisella novicida-like F6168. The plasmid was maintained in F. novicida Utah 112 and F. tularensis LVS strains. We sequenced the entire plasmid and found six open reading frames (ORFs)-ORF1, ORF2, ORF3, ORF4, ORF5, and ORFm. ORF3,...

journal_title：Plasmid

authors： Pomerantsev AP,Golovliov IR,Ohara Y,Mokrievich AN,Obuchi M,Norqvist A,Kuoppa K,Pavlov VM

更新日期：2001-11-01 00:00:00

abstract：:Coupling sequences are the 6 bp flanking the conjugative transposon Tn916 and are thought to play a role in determining the frequency of conjugative transposition. The affinity of binding of a chimeric protein, which consisted of maltose binding protein fused to the carboxy-terminal DNA binding domain of Tn916 integra...

journal_title：Plasmid

authors： Pethel B,Churchward G

更新日期：2000-03-01 00:00:00

abstract：:The complete nucleotide sequence of the cryptic plasmid pCC1 from Corynebacterium callunae (4109 bp) was determined. DNA sequence analysis revealed five open reading frames longer than 200 bp. One of the deduced polypeptides showed homology with the Rep proteins encoded by plasmids of the pIJ101/pJV1 family of plasmid...

journal_title：Plasmid

authors： Venkova-Canova T,Pátek M,Nesvera J

更新日期：2004-01-01 00:00:00

abstract：:Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...

journal_title：Plasmid

authors： D'Haeze W,Verplancke C,Mironov V,Holsters M

更新日期：2002-03-01 00:00:00

abstract：:Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurring compatible members of the Dictyostelium Ddp1 plasmid family found in the same wild isolate strain. The nucleotide sequences of Dmp1 (5983 bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading frames (ORFs) similar to the G1 and G5...

journal_title：Plasmid

authors： Kiyosawa H,Hughes JE,Welker DL

更新日期：1994-03-01 00:00:00

abstract：:Aeromonas hydrophila strain AO1 isolated from an infected fish was found to be resistant to several quinolones. A plasmid isolated from the strain AO1, termed pBRST7.6, was cloned and sequenced and shown to be 7621bp in length with a GC content of 60%. Further analysis confirmed that it contained a gene with 100% iden...

journal_title：Plasmid

authors： Majumdar T,Das B,Bhadra RK,Dam B,Mazumder S

更新日期：2011-07-01 00:00:00

abstract：:It is now known that conjugative pili are determined by representative plasmids for all incompatibility groups in Escherichia coli K-12. They fall into three basic morphological groups, which are described: thin flexible, thick flexible, and rigid filaments or rods. The main thrust of this study, however, has been the...

journal_title：Plasmid

authors： Bradley DE

更新日期：1980-09-01 00:00:00

abstract：UNLABELLED:This work describes a novel plasmid pSP187 (5550 bp) carrying the small multidrug resistance determinant smr encoding resistance to quaternary ammonium compounds (QACs). pSP187 was identified in a Staphylococcus pasteuri isolate recovered from bulk milk in a dairy cattle herd in Norway. Sequence analysis rev...

journal_title：Plasmid

authors： Bjorland J,Bratlie MS,Steinum T

更新日期：2007-03-01 00:00:00

abstract：:A small cryptic plasmid designated pHW15 was isolated from Rahnella genomospecies 2 WMR15 and its complete nucleotide sequence was determined. The plasmid contained 3002 bp with a G+C content of 47.4%. The origin of replication was identified by deletion analysis as a region of about 600 bp. This region had an identit...

journal_title：Plasmid

authors： Rozhon WM,Petutschnig EK,Jonak C

更新日期：2006-11-01 00:00:00

abstract：:We isolated a small multicopy cryptic plasmid, pNHK101, from Thermus sp. TK10 for use as a replicon of a Thermus expression vector. The nucleotide sequence of pNHK101 revealed that this plasmid was 1564bp long, with a total G+C content of 66.8%, which was in agreement with that of Thermus genomic DNA. The sequence did...

journal_title：Plasmid

authors： Kobayashi H,Kuwae A,Maseda H,Nakamura A,Hoshino T

更新日期：2005-07-01 00:00:00