Abstract:
:We have constructed shuttle vectors for integration of genes via double homologous recombination into three ectopic sites on the chromosome of Bacillus subtilis. The sites of integration are the pyrD, gltA, and sacA genes located at 139 degrees, 172 degrees, and 333 degrees, respectively, on the chromosome. Integration of the vectors into the target genes leads to antibiotic resistance as well as different metabolic phenotypes. B. subtilis strains with integrations of the empty vectors were able to sporulate at rates comparable to wild type cells. Similar levels of expression were obtained from constitutive lacZ fusions integrated at the different sites.
journal_name
Plasmidjournal_title
Plasmidauthors
Middleton R,Hofmeister Adoi
10.1016/j.plasmid.2004.01.006keywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
238-45issue
3eissn
0147-619Xissn
1095-9890pii
S0147619X04000332journal_volume
51pub_type
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