Abstract:
:We have evaluated the ability of immortalized human fibroblasts to recombine transfected plasmid DNA. A number of cell lines from normal individuals and from patients with DNA damage-processing defects were examined. Two plasmid recombination substrates were derived from pSV2neo and contained nonoverlapping deletions in the aminoglycoside phosphotransferase II gene. Intermolecular recombination was assessed by two methods after cotransfection. In a short-term, extrachromosomal recombination assay, low molecular weight DNA was extracted from the human cells 48 h after transfection, and recombinant plasmids were detected by transformation into appropriate indicator bacteria. In a long-term stable recombination assay the fibroblasts were cotransfected and G418-resistant colonies allowed to form. By the former assay all but two cultures were recombination-proficient, whereas all were recombination-proficient by the latter assay. The efficiency of transfection of human cells with plasmids appears to be a major variable affecting recombination. Recombination can be stimulated by uv irradiation of plasmid DNA prior to transfection. Cells from patients with Fanconi anemia, ataxia telangiectasia, and xeroderma pigmentosum complementation groups A, C, D, E, and G are not defective at intermolecular plasmid recombination.
journal_name
Plasmidjournal_title
Plasmidauthors
Timme TL,Wood CM,Moses REdoi
10.1016/0147-619x(89)90030-9subject
Has Abstractpub_date
1989-07-01 00:00:00pages
1-9issue
1eissn
0147-619Xissn
1095-9890pii
0147-619X(89)90030-9journal_volume
22pub_type
杂志文章相关文献
PLASMID文献大全abstract::Pinene is a monoterpene with wide industrial applications, especially as a promising high energy-density jet fuel. Traditional production of pinene on an industrial scale is material consumptive and has a low yield. As an alternative, microbial organisms have been engineered though advanced synthetic biological techni...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2019.102431
更新日期:2019-09-01 00:00:00
abstract::The Gram-positive Corynebacterium glutamicum is widely known for its application in the industrial production of amino acids and as a non-pathogenic model organism for cell wall biosynthesis in the group of CMN bacteria. For biotechnological and physiological studies often co-expression of recombinant genes is require...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2018.01.001
更新日期:2018-01-01 00:00:00
abstract::Polymerase chain reaction (PCR) is one of the most powerful tools in cloning genes. For the direct cloning of PCR products, T-vectors, which contain complementary 3'-thymidine overhangs, are widely used. In the present study, we developed a plasmid, pNB-T, which was constructed by cloning an XcmI cassette with a suffi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1500
更新日期:2001-01-01 00:00:00
abstract::The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(02)00100-2
更新日期:2002-09-01 00:00:00
abstract::We isolated a small multicopy cryptic plasmid, pNHK101, from Thermus sp. TK10 for use as a replicon of a Thermus expression vector. The nucleotide sequence of pNHK101 revealed that this plasmid was 1564bp long, with a total G+C content of 66.8%, which was in agreement with that of Thermus genomic DNA. The sequence did...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.12.004
更新日期:2005-07-01 00:00:00
abstract::A small cryptic plasmid designated pHW15 was isolated from Rahnella genomospecies 2 WMR15 and its complete nucleotide sequence was determined. The plasmid contained 3002 bp with a G+C content of 47.4%. The origin of replication was identified by deletion analysis as a region of about 600 bp. This region had an identit...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2006.05.007
更新日期:2006-11-01 00:00:00
abstract::Plasmid deletion formation was compared in wild-type, recE4, and addB72 strains of Bacillus subtilis. Deletion frequencies in plasmid pGP1, as monitored with a penP-lacZ fusion, were low in recE4 and high in addB72, in comparison to the wild-type strain. In the wild-type and recE4 strains, deletions between directly r...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(89)90044-9
更新日期:1989-05-01 00:00:00
abstract::We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb E...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1063
更新日期:1994-11-01 00:00:00
abstract::Transposon-insertion mutants with vir- Ti plasmids were characterized and then used in complementation experiments. One of the mutants (LBA 1517) had a mutation in a newly discovered vir locus called virF. The virF mutation led to a strongly diminished virulence on tomato and tobacco, but not on certain other plant sp...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(84)90026-x
更新日期:1984-05-01 00:00:00
abstract::The linear plasmid SCP1 of Streptomyces coelicolor A3(2) is one of the genetically more studied linear streptomycete replicons. Although the genetics of SCP1 and its interaction with the host chromosome have been analyzed for nearly three decades no information exists on its replication. With the help of an ordered co...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1419
更新日期:1999-11-01 00:00:00
abstract::Tn1935, a 23.5-kb transposon mediating resistance to ampicillin, kanamycin, mercury, spectinomycin, and sulfonamide was isolated from pZM3, an IncFIme virulence plasmid from Salmonella wien. Tn1935 possesses the entire sequence of Tn21 and contains two additional DNA segments of 0.95 and 2.7 kb carrying the ampicillin...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(88)90028-5
更新日期:1988-11-01 00:00:00
abstract::Horizontal transfer of genes encoding phenol degradation (pheBA) in the environment has been previously described. Complete or partial phe-operon was redetected in plasmids of several indigenous Pseudomonas strains isolated from the river water. The sequences of up- and downstream regions of the acquired phe-DNA in ei...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.07.003
更新日期:2004-11-01 00:00:00
abstract::Borrelia species comprise a unique genus of bacterial pathogens. These organisms contain a segmented genome with up to two dozen plasmids ranging in size from 5 kb up to about 200 kb. The plasmids have also been referred to as mini-chromosomes or essential genetic elements, as some of them carry information important ...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2013.05.002
更新日期:2013-09-01 00:00:00
abstract::To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2008.09.006
更新日期:2009-01-01 00:00:00
abstract::Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1289
更新日期:1997-01-01 00:00:00
abstract::Even though Vibrio cholerae is a well-known human pathogen, it is also a normal member of aquatic habitats. Within this environment it often forms biofilms on the chitin-containing exoskeleton of crustaceans and their molts. Chitin not only serves as nutrient source but also induces a developmental program called natu...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.08.001
更新日期:2010-11-01 00:00:00
abstract::The replication (rep) regions of small plasmids from three Sinorhizobium meliloti strains were cloned by marker rescue. Two unique replication regions were identified, one of which was common to two different strains. Plasmid pBB83 carried a 7.2 kbp rep region from a 42 kbp plasmid, and pBB84 carried a 4.5 kbp rep reg...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2005.08.003
更新日期:2006-03-01 00:00:00
abstract::The rapidly advancing field of plant synthetic biology requires transforming plants with multiple genes. This has sparked a growing interest in flexible plant transformation vectors, which can be used for multi-gene transformations. We have developed a novel binary vector series, named the PC-GW series (GenBank: KP826...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.06.003
更新日期:2015-09-01 00:00:00
abstract::A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90059-j
更新日期:1992-11-01 00:00:00
abstract::A preliminary screening study of six Moraxella catarrhalis isolates from primary school children in the Netherlands identified a small 3.5 kb plasmid (pEMCJH03), containing four open reading frames, which encoded three mobilizing and one replicase protein. Insertion of a kanamycin containing transposon (yielding pEMCJ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.11.003
更新日期:2005-05-01 00:00:00
abstract::Corynebacterium glutamicum is an industrial microorganism for production of amino acids. However, the metabolic engineering in C. glutamicum has been retarded due to lack of suitable vectors. In this study, we have constructed a shuttle vector pDXW-10 which harbors a large multiple cloning site suitable for cloning mu...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.05.004
更新日期:2010-09-01 00:00:00
abstract::Recently an insertional mutagenesis procedure has been developed to permit cloning of genes affected in developmental mutants of Dictyostelium discoideum (Kuspa and Loomis, Proc. Natl. Acad. Sci. USA 89, 8803-8807, 1992). In this procedure a plasmid bearing the URA (pyr5-6) gene is linearized with a restriction enzyme...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.0003
更新日期:1995-11-01 00:00:00
abstract::The fission yeast Schizosaccharomyces pombe is an attractive host for heterologous gene expression. However, expression systems for industrially viable large-scale fermentations are scarce. Several inducible expression vectors for S. pombe have been reported, with the strong thiamine-repressible nmt1+ promoter or deri...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2017.11.002
更新日期:2018-01-01 00:00:00
abstract::The conjugative transfer system of Yersinia enterocolitica 29930 present on the cryptic plasmid p29930 comprises a mating pore formation system (Mpf) related to that of the IncX plasmid R6K and a DNA transfer and replication system (Dtr) with close relationship to the mob region of the mobilizable plasmid CloDF13. Two...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.05.001
更新日期:2010-09-01 00:00:00
abstract::The mercury(II)-resistance transposons Tn502 and Tn512 were sequenced and shown to be members of the Tn5053 family. They are currently the sole representatives from the clinical setting and were obtained from geographically disparate Pseudomonas aeruginosa strains. The family is comprised of six novel transposons that...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.08.003
更新日期:2011-01-01 00:00:00
abstract::The incompatibility region of the Streptomyces plasmid pSL1 was identified as a 240-bp segment, though some other function from the vector plasmid was also necessary. A 540-bp fragment including the 240-bp region was enough for full expression of incompatibility. Inserted mutation analysis led to a more detailed locat...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(87)90021-7
更新日期:1987-03-01 00:00:00
abstract::Rolling circle-replicating plasmids constitute a vast family that is particularly abundant in, but not exclusive of, Gram-positive bacteria. These plasmids are constructed as cassettes that harbor genes involved in replication and its control, mobilization, resistance determinants and one or two origins of lagging str...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2014.05.004
更新日期:2014-07-01 00:00:00
abstract::We have modified an E. coli-staphylococcal shuttle vector for use in the general cloning and expression of genes from pathogenic staphylococci in Staphylococcus carnosus. As S. carnosus is non-pathogenic, this expression system will facilitate the study of the roles of individual gene products in the disease process. ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(02)00004-5
更新日期:2002-05-01 00:00:00
abstract::Little knowledge of DNA replication in cyanobacteria is available. In this study, we report the development and characterization of an in vitro system for studies of replication of the endogenous plasmids from the unicellular cyanobacterium Synechocystis 6803. This system (fraction III) was isolated at high salt conce...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1055
更新日期:1994-09-01 00:00:00
abstract::A procedure for the isolation of closed circular mitochondrial DNA (mtDNA) from cells permeabilized with digitonin is described. Compared to the standard procedure in which cells are broken after osmotic swelling, the digitonin-based procedure is more consistent and results in higher mtDNA yields. ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(86)90083-1
更新日期:1986-07-01 00:00:00