Abstract:
:The conjugative transfer system of Yersinia enterocolitica 29930 present on the cryptic plasmid p29930 comprises a mating pore formation system (Mpf) related to that of the IncX plasmid R6K and a DNA transfer and replication system (Dtr) with close relationship to the mob region of the mobilizable plasmid CloDF13. Two regions of the transfer system were selected for more detailed analyses of basic functions of conjugative transfer. The putative open reading frame orf22 located in the Mpf region confers the entry exclusion phenotype to possible recipient cells and inhibited conjugative transfer, when it was inserted into the coding region of the cat gene of pACYC184 in sense direction. Mobilization experiments with recombinant plasmids revealed that a 611bp fragment of the Dtr region containing two repeat sequences were required for a functional oriT by the conjugation system of Y. enterocolitica. While the conjugative transfer of cryptic plasmids harbouring the complete conjugation system had not been demonstrated previously, plasmid pBK17 containing the functional oriT was successfully mobilized from Y. enterocolitica strains into Escherichia coli, thus proving that the transfer system could contribute to the spread of these plasmids in nature.
journal_name
Plasmidjournal_title
Plasmidauthors
Kraushaar B,Appel B,Lanka E,Strauch Edoi
10.1016/j.plasmid.2010.05.001subject
Has Abstractpub_date
2010-09-01 00:00:00pages
79-84issue
2eissn
0147-619Xissn
1095-9890pii
S0147-619X(10)00033-8journal_volume
64pub_type
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