Coupling sequences flanking Tn916 do not determine the affinity of binding of integrase to the transposon ends and adjacent bacterial DNA.

Abstract:

:Coupling sequences are the 6 bp flanking the conjugative transposon Tn916 and are thought to play a role in determining the frequency of conjugative transposition. The affinity of binding of a chimeric protein, which consisted of maltose binding protein fused to the carboxy-terminal DNA binding domain of Tn916 integrase (Int), to different double-stranded oligonucleotide substrates containing coupling sequences associated with high- and low-frequency conjugative transposition was measured using a competition binding assay. The relative affinity of the chimeric protein was unaffected by the nature of the coupling sequences tested. The same results were obtained when the coupling sequences were placed in a different surrounding sequence context. It therefore appears that the effects of different coupling sequences on the frequency of conjugative transposition are not due simply to differences in Int binding.

journal_name

Plasmid

journal_title

Plasmid

authors

Pethel B,Churchward G

doi

10.1006/plas.1999.1440

keywords:

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

123-9

issue

2

eissn

0147-619X

issn

1095-9890

pii

S0147-619X(99)91440-3

journal_volume

43

pub_type

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