Abstract:
:Coupling sequences are the 6 bp flanking the conjugative transposon Tn916 and are thought to play a role in determining the frequency of conjugative transposition. The affinity of binding of a chimeric protein, which consisted of maltose binding protein fused to the carboxy-terminal DNA binding domain of Tn916 integrase (Int), to different double-stranded oligonucleotide substrates containing coupling sequences associated with high- and low-frequency conjugative transposition was measured using a competition binding assay. The relative affinity of the chimeric protein was unaffected by the nature of the coupling sequences tested. The same results were obtained when the coupling sequences were placed in a different surrounding sequence context. It therefore appears that the effects of different coupling sequences on the frequency of conjugative transposition are not due simply to differences in Int binding.
journal_name
Plasmidjournal_title
Plasmidauthors
Pethel B,Churchward Gdoi
10.1006/plas.1999.1440keywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
123-9issue
2eissn
0147-619Xissn
1095-9890pii
S0147-619X(99)91440-3journal_volume
43pub_type
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