Abstract：
:Heterologous expression in Escherichia coli is often limited by yield and solubility of the foreign protein in the bacterial cytoplasm. In many cases, overexpression also results in growth inhibition. In order to produce retroviral proteins that are especially difficult to overexpress in E. coli, we designed a set of beta-galactosidase fusion cassettes. Fusions with beta-galactosidase increase significantly both yield and solubility of the foreign proteins, thus making purification much easier. These cassettes allow for N- or C-terminal fusions, and the retroviral proteins can be released from the fusion by automaturation in vivo for the HIV-1 protease or cleavage by thrombine for Tat. More generally, any synthetic sequence coding for a given cleavage site can be introduced 5' or 3' to the lacZ gene through a convenient set of unique restriction sites, making these fusion cassettes highly versatile.
journal_name
Plasmidjournal_title
Plasmidauthors
Valverde V,Duplan H,Knibiehler M,Masson JMdoi
10.1006/plas.1994.1041subject
Has Abstractpub_date
1994-07-01 00:00:00pages
32-40issue
1eissn
0147-619Xissn
1095-9890pii
S0147-619X(84)71041-9journal_volume
32pub_type
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