Abstract:
:The nucleotide sequence of a smallest cryptic plasmid pRK10 of Serratia marcescens ACE2 was determined. When compared to the all other plasmids reported so far from S. marcescens in sizes of over 70kb, pRK10 is only 4241bp long with 53% G+C content and has five coding sequences representing a coding percentage of 65.41. This small plasmid consists of one Tdh gene, four mobilization genes, mobCABD, and an origin of replication homologous to those of ColE1-type plasmids. Analysis of the five open reading frames identified on the plasmid suggests the presence of genes involved in replication and mobilization containing sequences homologous to the bom region and mobCABD genes of ColE1 and Tdh from Acinetobacter baumannii str. AYE. Results also indicate that pRK10 does not encode any gene for antibiotic/heavy metal resistance. Copy number and incompatibility of the plasmid with plasmids of ColE1 origin of replication was determined and it is quite stable in its natural host as well as in Escherichia coli DH5alpha. This relatively small plasmid will be useful for construction of shuttle vectors to facilitate the genetic analysis.
journal_name
Plasmidjournal_title
Plasmidauthors
Syed Ibrahim K,Bakkiyaraj D,James R,Ganesh Babu T,Karutha Pandian STdoi
10.1016/j.plasmid.2009.08.001subject
Has Abstractpub_date
2009-11-01 00:00:00pages
183-90issue
3eissn
0147-619Xissn
1095-9890pii
S0147-619X(09)00095-Xjournal_volume
62pub_type
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